Structural studies of glycans isolated from rat plasma hemopexin.

abstract：PURPOSE:We evaluated the clinical relevance of microRNA-149 (miR-149) in neuroblastoma (NB) and its functional roles in regulating NB proliferation in vitro. METHODS:QRT-PCR was used to evaluate miR-149 expression in NB cell lines and primary NB tumors. Association between endogenous miR-149 expression in primary NB t...

journal_title：Biochimie

authors： Xu Y,Chen X,Lin L,Chen H,Yu S,Li D

更新日期：2017-08-01 00:00:00

abstract：:To elucidate the metabolic function of mRNA polyadenylation in Escherichia coli. we searched for a polyadenylate-binding protein as a potential mediator of the function of the poly(A) moiety. Using a nitrocellulose filter-binding assay and a Northwestern blot technique, a protein in the ribosomal supernatant fraction ...

journal_title：Biochimie

authors： Kalapos MP,Paulus H,Sarkar N

更新日期：1997-09-01 00:00:00

abstract：:The nuclear metabolism of poly(ADP-ribose) is mainly regulated by poly(ADP-ribose) polymerase-1 (PARP-1) and by poly(ADP-ribose) glycohydrolase (PARG). A PARP-like enzyme, V-PARP, and a PARG isoform are present in the extra-nuclear compartment of mammalian cells, even if poly(ADP-ribose) has never been detected therei...

journal_title：Biochimie

authors： Rossi L,Denegri M,Torti M,Poirier GG,Ivana Scovassi A

更新日期：2002-12-01 00:00:00

abstract：:Bacterial type II fatty acid synthesis (FASII) is a target for novel antibiotic development. All bacteria encode for mechanisms to incorporate exogenous fatty acids, and some bacteria can use exogenous fatty acids to bypass FASII inhibition. Bacteria encode three different mechanisms for activating exogenous fatty aci...

journal_title：Biochimie

authors： Yao J,Rock CO

更新日期：2017-10-01 00:00:00

abstract：:The kinetics of the native glycogen phosphorylase b from rabbit skeletal muscle and of the enzyme specifically deiminated by peptidylarginine deiminase have been studied. One arginine residue per phosphorylase b monomer is transformed into citrulline after 3 h of incubation with peptidylarginine deiminase. The maximal...

journal_title：Biochimie

authors： Eronina TB,Livanova NB,Chebotareva NA,Kurganov BI,Luo S,Graves DJ

更新日期：1996-01-01 00:00:00

abstract：:Sphingolipids are degraded by sphingomyelinase and ceramidase in the gut to ceramide and sphingosine, which may inhibit cell proliferation and induce apoptosis, and thus have anti-tumour effects in the gut. Although previous rodent studies including experiments on knockout mice indicate a role of neutral ceramidase in...

journal_title：Biochimie

authors： Ohlsson L,Palmberg C,Duan RD,Olsson M,Bergman T,Nilsson A

更新日期：2007-08-01 00:00:00

abstract：:The effect of bis-(p-nitrophenyl)phosphate on various glycosyltransferases involved in protein glycosylation (sialyl-, fucosyl-, galactosyl-, mannosyl- and glucosyltransferases) have been studied using crude enzyme preparations solubilized from rat spleen lymphocytes. Bis-(p-nitrophenyl)phosphate appears as a common i...

journal_title：Biochimie

authors： Guette F,Cacan R,Montreuil J,Verbert A

更新日期：1983-10-01 00:00:00

abstract：:Thyroid peroxidase (TPO) is the key enzyme involved in thyroid hormone synthesis. Autoantibodies to TPO (TPOAbs) are a hallmark of autoimmune thyroid disease (AITD). Here, we highlight recent progress over several years in understanding TPO biochemistry and function in various pathologies. TPO undergoes complex post-t...

journal_title：Biochimie

authors： Godlewska M,Banga PJ

更新日期：2019-05-01 00:00:00

abstract：:Genome-editing technologies, in particular, CRISPR systems, are widely used for targeted regulation of gene expression and obtaining modified human and animal cell lines, plants, fungi, and animals with preassigned features. Despite being well described and easy to perform, the most common methods for construction and...

journal_title：Biochimie

authors： Filippova J,Matveeva A,Zhuravlev E,Stepanov G

更新日期：2019-12-01 00:00:00

abstract：:SR-PSOX can function as a scavenger receptor, a chemokine and an adhesion molecule, and it could be an interesting player in the formation of atherosclerotic lesions. Our previous studies demonstrated that basic amino acid residues in the chemokine domain of SR-PSOX are critical for its functions. In this study the co...

journal_title：Biochimie

authors： Liu W,Yin L,Dai Y

更新日期：2013-02-01 00:00:00

abstract：:Dyskeratosis congenita is an inherited syndrome characterised by mucocutaneous features, bone marrow failure, an increased risk of malignancy and other somatic abnormalities. There is a considerable range of clinical severity and in its occult form the disease may present as idiopathic aplastic anaemia. Genes responsi...

journal_title：Biochimie

authors： Vulliamy TJ,Dokal I

更新日期：2008-01-01 00:00:00

abstract：:Synthetic antiestrogens such as Tamoxifen (Nolvadex) are currently used to treat postmenopausal breast cancer. Their mechanism of action is reviewed at the cellular and molecular level, through data of the literature and the current view of the author. Synthetic antiestrogens are mostly acting directly on breast cance...

journal_title：Biochimie

authors： Rochefort H

更新日期：1982-02-01 00:00:00

abstract：:Stereospecific hydrogenations according to the general scheme [formula: see text] are of interest from a preparative and mechanistic point of view. Proteus mirabilis is suitable for the hydrogenation of a-keto-acids to R-hydroxy-acids, and a Clostridium strain for the reduction of enoates. Both have been immobilized i...

journal_title：Biochimie

authors： Tischer W,Tiemeyer W,Simon H

更新日期：1980-01-01 00:00:00

abstract：:In 1998, the concept of "green chemistry" was established through twelve principles with the aim of improving the eco-efficiency of chemical processes and to judge, whether or not, a chemical process is sustainable. Currently, numerous processes do not obey to most of these principles (large energy usage, formation of...

journal_title：Biochimie

authors： Durand E,Lecomte J,Villeneuve P

更新日期：2016-01-01 00:00:00

abstract：:The specific binding of the amyloid protein precursor (APP) to glycosaminoglycans (GAG) suggests that APP is a cell adhesion molecule (CAM) and/or substrate adhesion molecule (SAM). In order to characterize this activity of APP in the brain at the molecular level, we have purified and characterized the major APP speci...

journal_title：Biochimie

authors： Multhaup G

更新日期：1994-01-01 00:00:00

abstract：:Mitochondria are essential organelles and consequently proper expression and maintenance of the mitochondrial genome are indispensable for proper cell function. The mitochondrial Suv3 (SUPV3L1) helicase is known to have a central role in mitochondrial RNA metabolism and to be essential for maintenance of mitochondrial...

journal_title：Biochimie

authors： Venø ST,Witt MB,Kulikowicz T,Bohr VA,Stevnsner T

更新日期：2015-01-01 00:00:00

abstract：:Cytochromes P450 constitute a superfamily of hemoproteins which have evolved from a common ancestor. Recent advances in molecular biology have offered a powerful approach to the research on the multiplicity of P450. Now, every mammalian P450 species can be characterized by heterologous expression of its cDNA. In addit...

journal_title：Biochimie

authors： Yabusaki Y

更新日期：1995-01-01 00:00:00

abstract：:The complete nucleotide sequence of a nerve growth factor precursor from Bothrops jararacussu snake (Bj-NGF) was determined by DNA sequencing of a clone from cDNA library prepared from the poly(A) + RNA of the venom gland of B. jararacussu. cDNA encoding Bj-NGF precursor contained 723 bp in length, which encoded a pre...

journal_title：Biochimie

authors： Kashima S,Soares AM,Roberto PG,Pereira JO,Astolfi-Filho S,Cintra AO,Fontes MR,Giglio JR,de Castro França S

更新日期：2002-07-01 00:00:00

abstract：:The antiviral activity of L-nucleoside analogs depends in part on the enantioselectivity of nucleoside kinases which catalyse their monophosphorylation. The substrate properties of human recombinant deoxycytidine kinase (dCK) and human recombinant deoxyguanosine kinase (dGK) with respect to L-adenosine and L-guanosine...

journal_title：Biochimie

authors： Gaubert G,Gosselin G,Boudou V,Imbach JL,Eriksson S,Maury G

更新日期：1999-11-01 00:00:00

abstract：:Our understanding of plant meiosis is rapidly increasing thanks to the model Arabidopsis thaliana. Here we present the results of a screening for meiotic mutants carried out with a library containing 30,719 T-DNA insertion lines. An average of one mutant per 1000 lines was recovered. Several phenotypic classes could b...

journal_title：Biochimie

authors： Mercier R,Grelon M,Vezon D,Horlow C,Pelletier G

更新日期：2001-11-01 00:00:00

abstract：:IbpA/B, 16 kDa heat-shock proteins were recently described as recognizing heterologous protein inclusion bodies in Escherichia coli cells; the corresponding genes formed an operon regulated by the rpoH gene product, sigma 32 protein (Burland et al (1993) Genomics 16, 551; Allen et al (1992) J Bacteriol 174, 6938; Chua...

journal_title：Biochimie

authors： Laskowska E,Wawrzynów A,Taylor A

更新日期：1996-01-01 00:00:00

abstract：BACKGROUND:The modest efficacy of available therapies for Hepatocellular carcinoma (HCC) indicates the need to develop novel therapeutic approaches. For the proteasome inhibitor Bortezomib (BZB), potentially attractive for HCC treatment, the mechanism of action is largely unknown. The BZB effect on E2Fs and the E2Fs co...

journal_title：Biochimie

authors： Farra R,Dapas B,Baiz D,Tonon F,Chiaretti S,Del Sal G,Rustighi A,Elvassore N,Pozzato G,Grassi M,Grassi G

更新日期：2015-05-01 00:00:00

abstract：:Rhizopus oryzae lipase (ROL) was found to be a true lipase. This enzyme presents the interfacial activation phenomenon. The N-terminal amino acid sequence of ROL was compared to those of rhizopus lipases. Purified ROL possesses the same N-terminal sequence as the mature Rhizopus niveus lipase (RNL). This sequence was ...

journal_title：Biochimie

authors： Ben Salah A,Sayari A,Verger R,Gargouri Y

更新日期：2001-06-01 00:00:00

abstract：:The dissociation of bound polyribosomes from ewe lactating mammary gland, by EDTA or Puromycin-KC1 treatment releases Messenger Ribonucleoprotein particles (mRNP'S) which sediment in sucrose gradients as a single component having a sedimentation coefficient of about 22S. RNAs derived from mRNPs sediment between 9S and...

journal_title：Biochimie

authors： Assairi L,Gaye P,Houdebine LM

更新日期：1976-01-01 00:00:00

abstract：:A nuclear poly(ADP-ribose) polymerase (PARP) is activated by gamma-irradiation and consequently synthesizes poly(ADP-ribose) by binding to DNA strand-breaks. This property suggests that PARP is a DNA strand-break-signal generator. Meanwhile, the cell-cycle arrest occurs in G1 and G2 phases following gamma-irradiation....

journal_title：Biochimie

authors： Masutani M,Nozaki T,Wakabayashi K,Sugimura T

更新日期：1995-01-01 00:00:00

abstract：:A human replication initiation protein, Cdt1, is a central player in the cell cycle regulation of DNA replication, and geminin down-regulates Cdt1 function by direct binding. It has been demonstrated that Cdt1 hyperfunction resulting from Cdt1-geminin imbalance, for example, by geminin silencing with small interfering...

journal_title：Biochimie

authors： Mizushina Y,Takeuchi T,Hada T,Maeda N,Sugawara F,Yoshida H,Fujita M

更新日期：2008-06-01 00:00:00

abstract：:The nucleotide sequence of the gene encoding chicken ribosomal protein L7a was determined. The gene contains eight exons and seven introns, which spread over 3613 nucleotides. The transcription initiation sites were located on four consecutive nucleotides GCCC, which are at the 5' terminus of a short polypyrimidine tr...

journal_title：Biochimie

authors： Maeda N,Kenmochi N,Tanaka T

更新日期：1993-01-01 00:00:00

abstract：:A thrombin-like enzyme, named BjussuSP-I, isolated from Bothrops jararacussu snake venom, is an acidic single-chain glycoprotein with M(r)=61,000, pI approximately 3.8 and 6% sugar. BjussuSP-I shows high proteolytic activity upon synthetic substrates, such as S-2238 and S-2288. It also shows procoagulant and kallikrei...

journal_title：Biochimie

authors： Sant'Ana CD,Bernardes CP,Izidoro LF,Mazzi MV,Soares SG,Fuly AL,Zingali RB,Magro AJ,Braz AS,Fontes MR,Stábeli RG,Sampaio SV,Soares AM

更新日期：2008-03-01 00:00:00

abstract：:In this study, peptidomics and genomics analyses were used to study antimicrobial peptides from the skin of Hylarana spinulosa. Twenty-nine different antimicrobial peptide precursors were characterized from the skin of H. spinulosa, which produce 23 mature antimicrobial peptides belonging to 12 different families. To ...

journal_title：Biochimie

authors： Yang X,Hu Y,Xu S,Hu Y,Meng H,Guo C,Liu Y,Liu J,Yu Z,Wang H

更新日期：2013-12-01 00:00:00

abstract：:Insects have developed an efficient host defense against microorganisms, which involves humoral and cellular mechanisms. Numerous data highlight similarities between defense responses of insects and innate immunity of mammals. The fruit fly, Drosophila melanogaster, is a favorable model system for the analysis of the ...

journal_title：Biochimie

authors： Levy F,Rabel D,Charlet M,Bulet P,Hoffmann JA,Ehret-Sabatier L

更新日期：2004-09-01 00:00:00