Abstract:
:The effect of bis-(p-nitrophenyl)phosphate on various glycosyltransferases involved in protein glycosylation (sialyl-, fucosyl-, galactosyl-, mannosyl- and glucosyltransferases) have been studied using crude enzyme preparations solubilized from rat spleen lymphocytes. Bis-(p-nitrophenyl)phosphate appears as a common inhibitor for every glycosyltransferase reaction utilizing sugar nucleotides as direct donors. In most cases 10 mM inhibitor is sufficient to obtain a 90 per cent inhibition. Kinetic studies achieved with a purified galactosyltransferase preparation reveal that bis-(p-nitrophenyl)phosphate exerts a competitive inhibition towards UDP-galactose binding. Concerning membrane-bound enzymes, the interaction of bis-(p-nitrophenyl)phosphate depends on its accessibility to the enzyme active site. This is shown by the different effect obtained with two UDP-Glc utilizing membrane-bound enzymes : UDP-Glc : phospho-dolichyl glucosyltransferase and UDP-Glc : ceramide glucosyltransferase : the first one not being affected but the second one being markedly inhibited under the same condition, although both are inhibited when the membrane environment is disturbed by detergent. Bis-(p-nitrophenyl)phosphate appears to be a tool to study membrane topology of glycosyltransferases.
journal_name
Biochimiejournal_title
Biochimieauthors
Guette F,Cacan R,Montreuil J,Verbert Adoi
10.1016/s0300-9084(83)80106-0subject
Has Abstractpub_date
1983-10-01 00:00:00pages
563-7issue
10eissn
0300-9084issn
1638-6183pii
S0300-9084(83)80106-0journal_volume
65pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::The 11 kb las locus, present on the 50 kb plasmid pCIM1, specifies the production of the lantibiotic lactocin S in Lactobacillus sakei L45. The gene cluster is organized into two oppositely orientated operons, lasAMNTUVPJW (lasA-W) and lasXY, the former of which contains the biosynthetic, immunity and transport genes....
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(02)01420-7
更新日期:2002-05-01 00:00:00
abstract::The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central ner...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.12.008
更新日期:2020-03-01 00:00:00
abstract::The binding of Ca2+ to membrane models composed of diplamitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylethanolamine (DPPE) 15N-labeled in the polar head group was investigated at pH 8.5 and pH 9.4 by 15N-NMR spectroscopy. Both phospholipids exhibit a decrease in the chemical shift anisotropy, indicating c...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(89)90143-0
更新日期:1989-01-01 00:00:00
abstract::Rhodospirillum salexigens is a moderately halophilic purple phototrophic bacterium which grows optimally in 8% NaCl. The amino acid sequences of the two principal soluble cytochromes c have been determined. One of these is a cytochrome c2, similar in size to mitochondrial cytochrome c. While clearly of the same sequen...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(94)90135-x
更新日期:1994-01-01 00:00:00
abstract::Mitochondria are essential organelles and consequently proper expression and maintenance of the mitochondrial genome are indispensable for proper cell function. The mitochondrial Suv3 (SUPV3L1) helicase is known to have a central role in mitochondrial RNA metabolism and to be essential for maintenance of mitochondrial...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.11.003
更新日期:2015-01-01 00:00:00
abstract::New strains of basidiomycetes producing extracellular laccases (Trametes ochracea 92-78, and Trametes hirsuta 56) have been found by screening of isolates of Trametes fungi. The laccases from T. hirsuta 56 and T. ochracea 92-78 as well as two laccases from previously found and described strains of basidiomycetes, name...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2004.08.005
更新日期:2004-09-01 00:00:00
abstract::The polysaccharide mixture obtained by hot water extraction of Aloe vahombe leaves is composed of at least four different paritally acetylated glucomannans which differ in molecular weight, glucose to mannose ratios and acetyl contents. Furthermore, one fraction contains a small but significant amount of protein which...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(86)80187-0
更新日期:1986-09-01 00:00:00
abstract::The bovine oxytocin precursor was expressed in Escherichia coli as a fusion protein by cloning the hormone encoding cDNA in frame behind the replicase gene of the RNA phage MS2. By step-wise extraction with different urea concentrations, the fusion protein was enriched in the 7 M urea fraction and further purified by ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90165-4
更新日期:1988-01-01 00:00:00
abstract::Excessive tissue iron levels are associated with the increase of oxidative/nitrative stress which contributes to tissue damage that may elevate the risk of diabetes. Therefore, we investigated the effects of iron on diabetes-associated liver injury and whether iron-related tyrosine nitration participated in this proce...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.07.019
更新日期:2012-12-01 00:00:00
abstract::The action of sialyltransferases (STs) on cell surface glycoconjugates is a key process in shaping cell phenotype in a variety of cells mostly involved in migratory and adhesive pathways. The factors determining cell-specific pattern of glycosylation are so far poorly understood. Most STs are resident proteins of the ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(03)00080-4
更新日期:2003-03-01 00:00:00
abstract::Berardinelli-Seip congenital lipodystrophy (BSCL) is a rare recessive disease characterized by near absence of adipose tissue and severe insulin resistance. In most cases, BSCL is due to loss-of-function mutations in the genes encoding either seipin of unknown function or 1-acyl-glycerol-3-phosphate O-acyltransferase ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.01.011
更新日期:2009-06-01 00:00:00
abstract::Pseudomonas cepacia 122 can grow on several lignin model compounds including the arylglycerol-beta-aryl ethers guaiacylglycerol-beta-coniferyl ether and guaiacylglycerol-beta-guaiacyl ether. Non-phenolic lignin model compounds are not degraded by this bacterium. The enzyme system catalyzing guaiacylglycerol-beta-guaia...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80047-x
更新日期:1985-02-01 00:00:00
abstract::Explants from 7, 8, 9, 11, 13-day chick embryonic skin incorporating (3H) Uridine for different periods 1 hr, 3 or 4 hr and a chase with actinomycin) are studied with respect to free (F) or membrane bound (B) cytoplasmic polysomes and to RNA extracted from them. Polysome specific activity decreases at older stages but...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80226-x
更新日期:1976-01-01 00:00:00
abstract::The published literature contains a number of examples of normally non-cytoplasmic proteins whose transport out of the cytoplasm is not completely abolished by drastic alterations to their routing signals (signal sequences, etc). Furthermore, there are numerous examples of cytoplasmic proteins that can be routed to an...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(90)90133-2
更新日期:1990-02-01 00:00:00
abstract::Sialidase catalyzes the removal of sialic acids from glycoconjugates. Recently, medaka sialidase Neu1 has been cloned and its enzymatic properties were investigated. Although enzymatic properties of this sialidase, such as optimal pH and substrate specificity, exhibits high similarity with human NEU1, Neu1 physiologic...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2017.01.008
更新日期:2017-04-01 00:00:00
abstract::tmRNA (10Sa RNA, ssrA) acts to rescue stalled bacterial ribosomes while encoding a peptide tag added trans-translationally to the nascent peptide, targeting it for proteolysis. Ribosomal protein S1 is required for tmRNA binding to isolated and poly U-programmed ribosomes. Mobility assays on native gels indicate that t...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(02)01442-6
更新日期:2002-08-01 00:00:00
abstract::Tryptocidine C (TpcC, cyclo[D-Phe1-Pro2-Trp3-D-Trp4-Asn5-Gln6-Trp7-Val8-Orn9-Leu10]) is a broad-spectrum antimicrobial peptide in the tyrothricin complex produced by a soil bacterium, Brevibacillus parabrevis. Electrospray mass spectrometric studies reveal the oligomerisation of TpcC into dimers and higher oligomers, ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.12.006
更新日期:2020-12-18 00:00:00
abstract::MicroRNAs (miRNAs) are ∼21 nt non-coding small RNAs which regulate gene expression at the post-transcriptional level in plants and animals. Until recently, only limited numbers of miRNAs were identified in Cucurbitaceae, a large flowering plant family. In this study, 220 potential miRNA candidates were identified from...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.03.002
更新日期:2014-07-01 00:00:00
abstract::The lysosomal aspartic protease cathepsin D (cath-D) is overexpressed and hyper-secreted by epithelial breast cancer cells. This protease is an independent marker of poor prognosis in breast cancer as it is correlated with the incidence of clinical metastasis. In normal cells, cath-D is localized in intracellular vesi...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2010.05.009
更新日期:2010-11-01 00:00:00
abstract::The targets of in vivo studies of the ribosomal assembly process are mainly the events of rRNA processing, whereas in vitro studies (total reconstitution) focus on principles of the assembly process such as assembly-initiation proteins, rate-limiting steps and a detailed sequence of assembly reactions (assembly map). ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(91)90054-5
更新日期:1991-06-01 00:00:00
abstract::Thraustochytrium is a marine protist producing a specific profile of nutritionally important fatty acids, including very long chain polyunsaturated fatty acids (VLCPUFAs) docosahexaenoic acid (DHA, 22:6n-3), even chain saturated fatty acids (SFAs) palmitic acid (16:0), and odd chain SFAs pentadecanoic acid (15:0). To ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2017.10.024
更新日期:2018-01-01 00:00:00
abstract::Five 4-arylcoumarins (1c-g) and twelve 3,4-dihydro-4-arylcoumarins (2a-l) were synthesized and tested for antioxidant activity, antitumor activity, toxicity and structure-activity relationships analysis. 4-Arylcoumarins and 3,4-dihydro-4-arylcoumarins that possess two hydroxyl groups in ortho position, such as 1d, 1f,...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.03.014
更新日期:2014-12-01 00:00:00
abstract::Concern about the malicious applications of botulinum neurotoxin has highlighted the need for a new generation of safe and highly potent antitoxins. In this study, we developed and evaluated the preclinical pharmacology and safety of a new F(ab')₂ antitoxin against botulinum neurotoxin serotype A (BoNT/A). As an alter...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.06.010
更新日期:2010-10-01 00:00:00
abstract::mRNAs extracted from human pheochromocytoma were translated in vitro in a lysate of a rabbit reticulocytes. Two enzymes of the biosynthetic pathway of the catecholamines, tyrosine-hydroxylase (TH) and dopamine-beta-hydroxylase (DBH), were characterized as translation products after immunoprecipitation by specific anti...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80198-x
更新日期:1985-06-01 00:00:00
abstract::Mouse brain myelin consists of a continuum of particles of different densities, as shown by sucrose density gradient centrifugation. In normal animals most of the material (65 per cent) is concentrated between 0.6 and 0.7 M sucrose (the maximum being found at 0.66 M sucrose, corresponding to 23 per cent). The density ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(77)80213-7
更新日期:1977-01-01 00:00:00
abstract::The phosphotriesterase PTE, identified in the soil bacterium Pseudomonas diminuta, is thought to have evolved in the last several decades to degrade the pesticide paraoxon with proficiency approaching the limit of substrate diffusion (k(cat)/K(M) of 4 x 10(7)M(-1)s(-1)). It belongs to the amidohydrolase superfamily, b...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2007.01.007
更新日期:2007-05-01 00:00:00
abstract::Fluorescence techniques were used to detect changes in the conformation of tRNA(Phe) that may occur during the peptidyl transferase reaction in which the tRNA appears to move between binding sites on ribosomes. Such a conformational change may be a fundamental part of the translocation mechanism by which tRNA and mRNA...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(87)90226-4
更新日期:1987-09-01 00:00:00
abstract::Parkinson's disease (PD), a neurodegenerative disorder, is characterized by a loss of dopaminergic neurons in the substantia nigra (SN) of the brain and it is well known that the pathogenesis of PD is related to a number of risk factors including oxidative stress. Antioxidant 1 (ATOX1) protein plays a crucial role in ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.10.010
更新日期:2019-01-01 00:00:00
abstract::Ten new defensins have been isolated from seeds of Triticum kiharae and related species of the Triticum and Aegilops genera by a combination of chromatographic procedures including affinity-, size-exclusion, and reversed-phase high-performance liquid chromatography. Nine were completely sequenced and shown to represen...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2006.09.009
更新日期:2007-05-01 00:00:00
abstract::Leishmania parasites cause two million new cases of leishmaniasis each year with several hundreds of millions of people at risk. Due to the paucity and shortcomings of available drugs, we have undertaken the crystal structure determination of a key enzyme from Leishmania major in hopes of creating a platform for the r...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.11.015
更新日期:2011-03-01 00:00:00