Abstract:
:The binding of Ca2+ to membrane models composed of diplamitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylethanolamine (DPPE) 15N-labeled in the polar head group was investigated at pH 8.5 and pH 9.4 by 15N-NMR spectroscopy. Both phospholipids exhibit a decrease in the chemical shift anisotropy, indicating changes of the order parameter of the C-N bond and decrease in half-height width. Binding of Ca2+ induces a chemical shift change for the DPPE signal which indicates a decrease in the pKa of the amino group. The binding of spermine was also investigated for mixed phase (DPPC/DPPE) at pH 8 and pH 9.4; a decrease in the DPPE pKa was also noted. The signals of both phospholipids are broadened and the line shapes are more complex because of the lower mobility and the higher steric bulk of this molecule. The results show the value of 15N-NMR in the study of mixed liposomes and indicate that the deprotonation of membrane surface could constitute a necessary step for fusion processes.
journal_name
Biochimiejournal_title
Biochimieauthors
Akoka S,Tellier Cdoi
10.1016/0300-9084(89)90143-0subject
Has Abstractpub_date
1989-01-01 00:00:00pages
137-44issue
1eissn
0300-9084issn
1638-6183pii
0300-9084(89)90143-0journal_volume
71pub_type
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