Abstract：
:The Rap family of small GTP-binding proteins is composed by four different members: Rap1A, Rap1B, Rap2A and Rap2B. In this work we report the identification and characterization of a fifth member of this family of small GTPases. This new protein is highly homologous to Rap2A and Rap2B, binds labeled GTP on nitrocellulose, and is recognized by a specific anti-Rap2 antibody, but not by an anti-Rap1 antibody. The protein has thus been named Rap2C. Binding of GTP to recombinant purified Rap2C was Mg(2+)-dependent. However, accurate comparison of the kinetics of nucleotide binding and release revealed that Rap2C bound GTP less efficiently and possessed slower rate of GDP release compared to the highly homologous Rap2B. Moreover, in the presence of Mg(2+), the relative affinity of Rap2C for GTP was only about twofold higher than that for GDP, while, under the same conditions, Rap2B was able to bind GTP with about sevenfold higher affinity than GDP. When expressed in eukaryotic cells, Rap2C localized at the plasma membrane, as dictated by the presence of a CAAX motif at the C-terminus. We found that Rap2C represented the predominant Rap2 protein expressed in circulating mononuclear leukocytes, but was not present in platelets. Importantly, Rap2C was found to be expressed in human megakaryocytes, suggesting that the protein may be down-regulated during platelets generation. This work demonstrates that Rap2C is a new member of the Rap2 subfamily of proteins, able to bind guanine nucleotides with peculiar properties, and differently expressed by various hematopoietic subsets. This new protein may therefore contribute to the still poorly clarified cellular events regulated by this subfamily of GTP-binding proteins.
journal_name
Biochimiejournal_title
Biochimieauthors
Paganini S,Guidetti GF,Catricalà S,Trionfini P,Panelli S,Balduini C,Torti Mdoi
10.1016/j.biochi.2005.08.007keywords:
subject
Has Abstractpub_date
2006-03-01 00:00:00pages
285-95issue
3-4eissn
0300-9084issn
1638-6183pii
S0300-9084(05)00200-2journal_volume
88pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:The SOS response can be induced indirectly in Escherichia coli by infection with UV irradiated bacteriophage P1, lambda or M13. Induction, monitored quantitatively by means of a sfiA::lac operon fusion, was stronger with the plasmid phage P1 than with lambda, but the kinetics were similar, showing that plasmid and non...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(82)80100-4
更新日期：1982-08-01 00:00:00
abstract：:The combination of cDNA and RNA sequencing techniques has enabled determination of the complete sequence of one of the mRNAs coding for the precursor of the small subunit of ribulose bisphosphate carboxylase of Nicotiana sylvestris. In this 898-nucleotide-long mRNA, 540 nucleotides code for the entire 180-amino-acid-l...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(84)90148-2
更新日期：1984-07-01 00:00:00
abstract：:NikR proteins are bacterial metallo-regulatory transcription factors that control the expression of the nickel uptake system and/or nickel containing enzymes such as urease, and are involved in the acid stress response. Here, a comparative study is reported on NikR from Helicobacter pylori (HpNikR) and Escherichia col...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2006.07.016
更新日期：2006-11-01 00:00:00
abstract：:Human mitochondrial diseases, defined as the diseases due to a mitochondrial oxidative phosphorylation defect, represent a large group of very diverse diseases with respect to phenotype and genetic causes. They present with many unsolved issues, the comprehensive analysis of which is beyond the scope of this review. W...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2013.08.012
更新日期：2014-05-01 00:00:00
abstract：:We have studied through the late foetal period of the rat, the evolution of two enzymes involved in glycogen metabolism of the liver : UDPG glycogen synthetase (a and b forms) and branching enzyme. The activities were measured during the development of normal foetuses and of foetuses experimentally deprived of cortico...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(75)80140-4
更新日期：1975-01-01 00:00:00
abstract：:Previous work from this laboratory has shown that T7 RNA polymerase outpaces ribosomes in vivo, generating naked mRNA stretches which may be nuclease-sensitive. In particular, lacZ transcripts synthesised this way are highly unstable and yield little beta-galactosidase. We have argued that most of these transcripts ar...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(96)84747-x
更新日期：1996-01-01 00:00:00
abstract：:In man the brain represents about 2% of the body weight, but contains 25% of the body's cholesterol. Cholesterol itself does not cross the blood-brain barrier and is synthesised in situ. Excess cholesterol from brain is exported in the form of oxysterols, or metabolised to steroids, which in contrast to cholesterol ca...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2006.10.008
更新日期：2007-02-01 00:00:00
abstract：:Epigenetic mechanisms are likely to play a role in many complex diseases, the extent of which we only beginning to understand. COPD and asthma are two respiratory diseases subject to strong environmental influences depending on underlying genetic susceptibility. Epigenetic mechanisms such as DNA methylation, histone m...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2012.07.017
更新日期：2012-11-01 00:00:00
abstract：:Kinetic parameters characterizing the catalytic activities of enzymes are typically investigated in dilute solutions. However, in reality, these reactions occur in cells that, in addition to water and ions, are full of other macromolecules including proteins, nucleic acids, lipids, and metabolites. Such a crowded envi...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.07.009
更新日期：2020-09-01 00:00:00
abstract：:Glutaredoxins (Grxs), redox-active proteins with a typical -CPYC motif at their active sites, are involved in redox-regulatory processes and antioxidant defenses. The human malarial parasite Plasmodium falciparum possess a classical glutaredoxin (PfGrx) as well as a number of Grx-like proteins. In the present study, w...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2009.12.003
更新日期：2010-03-01 00:00:00
abstract：:Conformational flexibility and structural fluctuations play an important role in enzyme activity. A great variety of internal motions ranging over different time scales and of different amplitudes are involved in the catalytic cycle. These different types of motions and their functional consequences are considered in ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(98)80054-0
更新日期：1998-01-01 00:00:00
abstract：:The lactose plasmid pUCL22 of the single plasmid strain Streptococcus lactis ssp. lactis Z270 was demonstrated to fuse with the heterologous conjugative plasmid pVA797. The fusion of pUCL22 with pVA797 occurred by recombination between a specific sequence of pUCL22 and different sites of pVA797. The cointegrates of pU...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(88)90091-0
更新日期：1988-04-01 00:00:00
abstract：:The bovine oxytocin precursor was expressed in Escherichia coli as a fusion protein by cloning the hormone encoding cDNA in frame behind the replicase gene of the RNA phage MS2. By step-wise extraction with different urea concentrations, the fusion protein was enriched in the 7 M urea fraction and further purified by ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(88)90165-4
更新日期：1988-01-01 00:00:00
abstract：:Apoptosis is the physiological process by which unwanted cells in an organism are killed. Bcl-2, a membrane-bound cytoplasmic protein, and its close relative Bcl-xL, are both effective inhibitors of apoptosis induced by a wide variety of stimuli in many different cell types. In a previous study, we reported that suppr...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(97)82011-1
更新日期：1997-10-01 00:00:00
abstract：:In 1956, when we started our collaboration, both Alick Isaacs and myself had done previous work on interference between inactive and active influenza viruses. We were aware of the state interference research had reached and of the two alternative explanations that had been envisaged. ...
journal_title：Biochimie
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.biochi.2007.04.007
更新日期：2007-06-01 00:00:00
abstract：:Recombinant plasmids containing head-to-tail copies of different coat-protein replacement genomes of wheat dwarf virus (WDV) were used to study the mechanism leading to the release of replicating unit-length molecules in suspension culture cells of Triticum monococcum. For plasmids bearing two complete genomes, the vi...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(93)90067-3
更新日期：1993-01-01 00:00:00
abstract：:The study of modified nucleoside contributions to RNA chemistry, structure and function has been thwarted by the lack of a site-selected method of incorporation which is both versatile and adaptable to present synthetic technologies. A reproducible and versatile site-selected incorporation of nine differently modified...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(96)88115-6
更新日期：1995-01-01 00:00:00
abstract：:Insects have developed an efficient host defense against microorganisms, which involves humoral and cellular mechanisms. Numerous data highlight similarities between defense responses of insects and innate immunity of mammals. The fruit fly, Drosophila melanogaster, is a favorable model system for the analysis of the ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2004.07.007
更新日期：2004-09-01 00:00:00
abstract：:Two-dimensional J-resolved and correlated 1H NMR spectra with complete water suppression have been obtained to further characterize a metabolic pattern for normal and pathological human plasma samples. 1H COSY spectra have been recorded on plasma from 12 patients with cancer in order to check for the possible presence...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(92)90075-p
更新日期：1992-09-01 00:00:00
abstract：:The variable manifestation of phenotypes that occur in patients with neurofibromatosis type 1 (NF1) includes benign and malignant neurocutaneous tumors for which no adequate treatment exists. Cell-based screening of known bioactive compounds library identified the protein phosphatase 2A (PP2A) inhibitor Cantharidin an...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.01.001
更新日期：2017-04-01 00:00:00
abstract：:Thraustochytrium is a marine protist producing a specific profile of nutritionally important fatty acids, including very long chain polyunsaturated fatty acids (VLCPUFAs) docosahexaenoic acid (DHA, 22:6n-3), even chain saturated fatty acids (SFAs) palmitic acid (16:0), and odd chain SFAs pentadecanoic acid (15:0). To ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.10.024
更新日期：2018-01-01 00:00:00
abstract：:A d-carbamoylase from Sinorhizobium morelens S-5 was purified and characterized. The enzyme was purified 189-fold to homogeneity with a yield of 19.1% by aqueous two-phase extraction and two steps of column chromatography. The enzyme is a homotetramer with a native molecular mass of 150 kDa and a subunit relative mole...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.05.013
更新日期：2006-03-01 00:00:00
abstract：:Catalase (E.C 1.11.1.6) was purified from leaves of Zandedeschia aethiopica to apparent homogeneity by a one-step hydrophobic interaction chromatography on a phenyl Sepharose CL-4B column. The purified enzyme preparation was obtained with a final recovery of enzyme activity of about 61% and a specific activity of 146 ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(88)90035-1
更新日期：1988-12-01 00:00:00
abstract：:All hormones and neurotransmitters which provoke their intracellular effects by increasing the cytosolic concentration of Ca2+ in their target cells also stimulate the breakdown of inositol phospholipids. Much evidence suggests that this breakdown is intimately involved in the mechanism which couples cell-surface rece...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(85)80115-2
更新日期：1985-10-01 00:00:00
abstract：:The SOS response is induced in E coli following treatments that interfere with DNA replication. The response is under the control of the recA and the lexA genes. Strains defective in LexA repressor constitutively express SOS proteins. However, SOS repair does not reach its maximum level in these strains. Instead, an a...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(91)90107-c
更新日期：1991-04-01 00:00:00
abstract：:Epithelial Ca(2+)-regulation, which governs cornified envelope formation in the skin epidermis and hair follicles, closely coincides with the expression of S100A3, filaggrin and trichohyalin, and the post-translational modification of these proteins by Ca(2+)-dependent peptidylarginine deiminases. This review summariz...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2011.05.028
更新日期：2011-12-01 00:00:00
abstract：:In the bacterium Escherichia coli, H-NS-(H1, H1a) is a heat-stable protein with a molecular mass of 15.5 kDa involved in nucleoid organisation and gene regulation linked to certain signal transduction pathways. We have shown that, following addition of preparations of everted inner membrane vesicles, heat-stable cleav...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(97)80025-9
更新日期：1997-06-01 00:00:00
abstract：:Mitochondrial function requires genes encoded in both mitochondrial and nuclear genomes. Tfam, the activator of mammalian mitochondrial transcription, is encoded in the nucleus and its expression has been shown in in vitro studies to be controlled by nuclear respiratory factors NRF-1 and NRF-2. In order to understand ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(99)00223-0
更新日期：1999-10-01 00:00:00
abstract：:Poly(ADP-ribosyl)ation is a posttranslational protein modification significant for genomic stability and cell survival in response to DNA damage. Poly(ADP-ribosyl)ation is catalyzed by poly(ADP-ribose)polymerases (PARPs). Among the 17 members of the PARP family, PARP-1 and PARP-2 are described as enzymes whose catalyt...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.01.007
更新日期：2013-06-01 00:00:00
abstract：:Several mechanistic hypotheses have been proposed for how carotenoid pigmentation of integumentary structures can serve as an honest signal of individual quality. These hypotheses are founded on proposed links between carotenoids, immuno responsiveness, and oxidative stress, but an absence of biochemical information o...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.10.021
更新日期：2013-02-01 00:00:00
