NK-18, a promising antimicrobial peptide: anti-multidrug resistant leukemia cells and LPS neutralizing properties.

abstract：:The chemical carcinogen N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) induces frameshift mutations located within two types of specific sequences (mutation hot spots): i) contiguous guanine sequences and ii) alternating GC sequences. The genetic requirements of these frameshift events were investigated using specific ...

journal_title：Biochimie

authors： Janel-Bintz R,Maenhaut-Michel G,Takahashi M,Fuchs RP

更新日期：1991-04-01 00:00:00

abstract：:The nucleotide sequence of the gene encoding chicken ribosomal protein L7a was determined. The gene contains eight exons and seven introns, which spread over 3613 nucleotides. The transcription initiation sites were located on four consecutive nucleotides GCCC, which are at the 5' terminus of a short polypyrimidine tr...

journal_title：Biochimie

authors： Maeda N,Kenmochi N,Tanaka T

更新日期：1993-01-01 00:00:00

abstract：:The cartilage tissue has a limited self-regenerative capacity. Tissue-engineering represents a promising trend for cartilage repair. The present study was aimed to develop a biomaterial formulation by combining fragments of chitosan hydrogel with isolated rabbit or human chondrocytes. We first reported the properties ...

journal_title：Biochimie

authors： Montembault A,Tahiri K,Korwin-Zmijowska C,Chevalier X,Corvol MT,Domard A

更新日期：2006-05-01 00:00:00

abstract：:The hypothesis is proposed that pre-biotic bacterial cell(s) and the first cells capable of growth/division did not require a cytoplasmic membrane. A gel-like microscopic structure less than a cubic micrometer may have had a dual role as both an ancient pre-cytoplasm and a boundary layer to the higher-entropy external...

journal_title：Biochimie

authors： Trevors JT,Pollack GH

更新日期：2012-01-01 00:00:00

abstract：:Honeybee-plant relationships are based on a conditioning process in which olfactory (plant aroma) and gustatory cues (mainly nectars) are closely linked, leading to a selective foraging behaviour. Among crops dependent upon entomophilous cross-pollination, the sunflower has recently undergone extensive expansion due t...

journal_title：Biochimie

authors： Pham-Delegue MH,Etievant P,Masson C

更新日期：1987-06-01 00:00:00

abstract：:Five 4-arylcoumarins (1c-g) and twelve 3,4-dihydro-4-arylcoumarins (2a-l) were synthesized and tested for antioxidant activity, antitumor activity, toxicity and structure-activity relationships analysis. 4-Arylcoumarins and 3,4-dihydro-4-arylcoumarins that possess two hydroxyl groups in ortho position, such as 1d, 1f,...

journal_title：Biochimie

authors： Zhang K,Ding W,Sun J,Zhang B,Lu F,Lai R,Zou Y,Yedid G

更新日期：2014-12-01 00:00:00

abstract：:Disappearance of 7-ethylguanine and O6-ethylguanine lesions from nuclear DNA is observed on incubation of isolated rat liver nuclei previously treated with ethylnitrosourea. Free 7-ethylguanine but no free O6-ethylguanine is released in the medium. Incubation of methylated or ethylated DNA with chromatin proteins lead...

journal_title：Biochimie

authors： Renard A,Lemaitre M,Verly WG

更新日期：1982-08-01 00:00:00

abstract：:Tri(n-butyl)phosphate (TNBP) and sodium cholate (SC) mixtures have been used to inactivate lipid-enveloped viruses like HIV and hepatitis B. We exploited the use of this combination to purify fibroblast growth factor-2 (FGF-2) from human placenta. Human placentas were extracted in the presence of 0.3% TNBP/0.2% SC and...

journal_title：Biochimie

authors： Wolff P,Tanaka AM,Chenker E,Cabrera-Crespo J,Raw I,Ho PL

更新日期：1996-01-01 00:00:00

abstract：:A human replication initiation protein, Cdt1, is a central player in the cell cycle regulation of DNA replication, and geminin down-regulates Cdt1 function by direct binding. It has been demonstrated that Cdt1 hyperfunction resulting from Cdt1-geminin imbalance, for example, by geminin silencing with small interfering...

journal_title：Biochimie

authors： Mizushina Y,Takeuchi T,Hada T,Maeda N,Sugawara F,Yoshida H,Fujita M

更新日期：2008-06-01 00:00:00

abstract：:Various phosphonic and sulfonic glutamate analogues as well as phosphonopeptides related to glutathione were studied for their interaction with rat kidney gamma-glutamylcysteine synthetase activity. We found, in all cases, that the presence of a phosphonic group increases the affinity for the enzyme. Among the tripept...

journal_title：Biochimie

authors： Marche M,Basurko MJ,Cassaigne A

更新日期：1987-05-01 00:00:00

abstract：:The effect of bis-(p-nitrophenyl)phosphate on various glycosyltransferases involved in protein glycosylation (sialyl-, fucosyl-, galactosyl-, mannosyl- and glucosyltransferases) have been studied using crude enzyme preparations solubilized from rat spleen lymphocytes. Bis-(p-nitrophenyl)phosphate appears as a common i...

journal_title：Biochimie

authors： Guette F,Cacan R,Montreuil J,Verbert A

更新日期：1983-10-01 00:00:00

abstract：:We present here, a new method for coupling sulfhydryl groups (SH) to low-density lipoprotein (LDL) surface. This method uses homocysteine thiolactone (HCTL) which reacts with lysine residues in a very mild manner, and permits the selection of the number of SH bound per LDL. Under our experimental conditions (8 SH/LDL)...

journal_title：Biochimie

authors： Vidal M,Sainte-Marie J,Philippot J,Bienvenue A

更新日期：1986-05-01 00:00:00

abstract：:Total poly (A+) RNA has been extracted from rat pheochromocytoma and translated in vitro by means of a reticulocyte lysate system. We show that two antisera, prepared against pig kidney DOPA decarboxylase (DDC) or rat pheochromocytoma DDC, immunoprecipitate an in vitro synthetized 50 kDa polypeptide identified as DDC ...

journal_title：Biochimie

authors： Bruneau G,Krieger-Poullet M,Coge F,Borri-Voltattorni C,Gros F,Thibault J

更新日期：1990-01-01 00:00:00

abstract：:A variety of transcription attenuation mechanisms are used by bacteria to regulate gene and operon expression. This review summarizes previous and current studies designed to elucidate the features of the specific attenuation mechanisms that regulate expression of the tryptophanase (tna) operon of Escherichia coli and...

journal_title：Biochimie

authors： Yanofsky C,Konan KV,Sarsero JP

更新日期：1996-01-01 00:00:00

abstract：:Thyroid peroxidase is a member of a family of mammalian peroxidases that includes myeloperoxidase, lactoperoxidase, eosinophil peroxidase, and salivary peroxidase. Protein sequences showing a high degree of sequence similarity with mammalian peroxidases have recently been observed in several invertebrate species. A mu...

journal_title：Biochimie

authors： Taurog A

更新日期：1999-05-01 00:00:00

abstract：:The repressible Saccharomyces cerevisiae acid phosphatase (APase) coded by the PHO5 gene is a cell wall protein that follows the yeast secretory pathway. We had previously described the in vivo fate of a multicopy plasmid-encoded modified protein, lacking 15 out of 17 signal peptide amino acids. This modified protein ...

journal_title：Biochimie

authors： Silve S,Volland C,Hinnen A,Haguenauer-Tsapis R

更新日期：1990-02-01 00:00:00

abstract：:The interactions of DNA with histone H4 and with its fragments N-H4 (1-84) and C-H4 (85-102) have been studied by using electrooptical techniques, viscosity and electron microscopy. Electron microscopy reveals that histone H4 induces a large folding of DNA molecules : this is in agreement with electrooptical measureme...

journal_title：Biochimie

authors： Palau AO,Mozo A,Querol E

更新日期：1980-01-01 00:00:00

abstract：:Theoretical and experimental investigations on the conformations of synthetic polytripeptides, with Gly-R2-R3 general repetitive sequence, are of great interest for the comprehension of the factors which contribute to the triple helix stabilization. Using appropriate molecules as models, we have investigated by the PC...

journal_title：Biochimie

authors： Cabrol D,Broch H,Vasilescu D

更新日期：1981-11-01 00:00:00

abstract：:Obesity prevalence continues to rise throughout the developed world, as a result of positive energy balance and reduced physical activity. At present, there is still a perception within the general community, and amongst some nutritionists, that eating multiple small meals spaced throughout the day is beneficial for w...

journal_title：Biochimie

authors： Hutchison AT,Heilbronn LK

更新日期：2016-05-01 00:00:00

abstract：:After the assessment of the antitumor activity of the anthracycline pigments, the S peucetius group of metabolites was discovered and eventually doxorubicin, a major anticancer agent of established clinical usefulness was developed in the early seventies. A second generation of compounds followed, represented mainly b...

journal_title：Biochimie

authors： Arcamone FM

更新日期：1998-03-01 00:00:00

abstract：:A soluble hydrogenase from the halophilic sulfate reducing bacterium Desulfovibrio salexigens, strain British Guiana (NCIB 8403) has been purified to apparent homogeneity with a final specific activity of 760 mumoles H2 evolved/min/mg (an overall 180-fold purification with 20% recovery yield). The enzyme is composed o...

journal_title：Biochimie

authors： Teixeira M,Moura I,Fauque G,Czechowski M,Berlier Y,Lespinat PA,Le Gall J,Xavier AV,Moura JJ

更新日期：1986-01-01 00:00:00

abstract：:As part of a project aimed at obtaining selective inhibitors and drug-like compounds targeting tRNA synthetases from trypanosomatids, we have elucidated the crystal structure of human cytosolic histidyl-tRNA synthetase (Hs-cHisRS) in complex with histidine in order to be able to compare human and parasite enzymes. The...

journal_title：Biochimie

authors： Koh CY,Wetzel AB,de van der Schueren WJ,Hol WG

更新日期：2014-11-01 00:00:00

abstract：:The inhibition rates and spectral characteristics of 2 probes specific for the active-site serine residue of proteases were examined for evidence of conformational change of the proteases upon their binding to alpha 2-macroglobulin (alpha 2M). Elastase, chymotrypsin, trypsin, and plasmin were reacted with (7-nitrobenz...

journal_title：Biochimie

authors： Martini JL,Pochon F

更新日期：1989-03-01 00:00:00

abstract：:Three Plasmodium falciparum serine repeat antigen (SERA) protein peptides were studied by NMR and structure calculations being done in 70:30 water:trifluoroethanol solution. Peptide 22834 was shown to be immunogenic and protective against malaria in Aotus monkeys, whilst native peptide 6737 and its analogue 14096 did ...

journal_title：Biochimie

authors： Cubillos M,Alba MP,Bermúdez A,Trujillo M,Patarroyo ME

更新日期：2003-07-01 00:00:00

abstract：:The periplasmic histidine permease of Salmonella typhimurium is composed of a soluble histidine-binding protein and three membrane-bound components. These latter are produced in very small amounts and only two, the Q and the P protein, have been previously identified. This paper describes the construction of a plasmid...

journal_title：Biochimie

authors： Ames GF,Nikaido K,Hobson A,Malcolm B

更新日期：1985-01-01 00:00:00

abstract：:The Cystic Fibrosis Transmembrane Regulator (CFTR) is a membrane protein whose mutations cause cystic fibrosis, a lethal genetic disease. We performed a molecular dynamic (MD) study of the properties of the nucleotide binding domains (NBD) whose conformational changes, upon ATP binding, are the direct responsible of t...

journal_title：Biochimie

authors： Belmonte L,Moran O

更新日期：2015-04-01 00:00:00

abstract：:The chromatin of shrimp hepatopancreas has been extracted from isolated nuclei and characterized. Nuclei were prepared in the presence of Cu++ and phenyl methyl sulfonyl fluoride in order to inhibit the nuclease and protease activities throughout the different purification steps. The purified nuclei are heterogenous i...

journal_title：Biochimie

authors： Sellos D,Van Wormhoudt A

更新日期：1979-01-01 00:00:00

abstract：:We identified a T-to-C mutation 2 nucleotides (nt) upstream from the AG in a GT-AG intron between exons 2 and 3 in the human T-cell leukemia virus type I (HTLV-I) tax mRNA. This mutation resulted in the preferential usage of an alternative splice site, causing a 75-nt elongation of tax mRNA and reduced production of v...

journal_title：Biochimie

authors： Ohsugi T

更新日期：2006-02-01 00:00:00

abstract：:Analysis of the nucleotide sequence of 1,400 transfer RNAs has revealed the imprint of a prototypic genetic code in position 3-4-5 of the acceptor stem. It appears only in the transfer RNAs for the primordial amino acids ie those found by chemical condensation of a nitrogen-methane-water-ammonia mixture. The model for...

journal_title：Biochimie

authors： Möller W,Janssen GM

更新日期：1990-05-01 00:00:00

abstract：:In vitro cycling rates of E. coli ribosomes and of elongation factors EF-Tu and EF-G have been obtained and these are compatible with translation rates in vivo. We show that the rate of translocation is faster than 50 s-1 and therefore that the EF-G function is not a rate limiting step in protein synthesis. The in viv...

journal_title：Biochimie

authors： Bilgin N,Kirsebom LA,Ehrenberg M,Kurland CG

更新日期：1988-05-01 00:00:00