Abstract：
:Tri(n-butyl)phosphate (TNBP) and sodium cholate (SC) mixtures have been used to inactivate lipid-enveloped viruses like HIV and hepatitis B. We exploited the use of this combination to purify fibroblast growth factor-2 (FGF-2) from human placenta. Human placentas were extracted in the presence of 0.3% TNBP/0.2% SC and the clarified homogenate was adsorbed to S-Sepharose. The active fractions were further loaded onto a heparin-Sepharose column and purified FGF-2 was eluted with 2.0 M NaCl. FGF-2 purified this way was indistinguishable from FGF-2 purified without TNBP/SC in the extraction step in terms of yield, specific activity and biological response. The lipid-enveloped vaccinia virus was used in a parallel experiment to evaluate the inactivation capacity of our protocol. Under the conditions described here, the combined use of TNBP/SC did not eliminate but reduced significantly the number of vaccinia virus PFUs by log 2-3.
journal_name
Biochimiejournal_title
Biochimieauthors
Wolff P,Tanaka AM,Chenker E,Cabrera-Crespo J,Raw I,Ho PLdoi
10.1016/0300-9084(96)89504-6subject
Has Abstractpub_date
1996-01-01 00:00:00pages
190-4issue
3eissn
0300-9084issn
1638-6183pii
0300908496895046journal_volume
78pub_type
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