Abstract：
:Some physico-chemical properties of R140G and K141Q mutants of human small heat shock protein HspB1 associated with hereditary peripheral neuropathy were analyzed. Mutation K141Q did not affect intrinsic Trp fluorescence and interaction with hydrophobic probe bis-ANS, whereas mutation R140G decreased both intrinsic fluorescence and fluorescence of bis-ANS bound to HspB1. Both mutations decreased thermal stability of HspB1. Mutation R140G increased, whereas mutation K141Q decreased the rate of trypsinolysis of the central part (residues 5-188) of HspB1. Both the wild type HspB1 and its K141Q mutant formed large oligomers with apparent molecular weight ∼560 kDa. The R140G mutant formed two types of oligomers, i.e. large oligomers tending to aggregate and small oligomers with apparent molecular weight ∼70 kDa. The wild type HspB1 formed mixed homooligomers with R140G mutant with apparent molecular weight ∼610 kDa. The R140G mutant was unable to form high molecular weight heterooligomers with HspB6, whereas the K141Q mutant formed two types of heterooligomers with HspB6. In vitro measured chaperone-like activity of the wild type HspB1 was comparable with that of K141Q mutant and was much higher than that of R140G mutant. Mutations of homologous hot-spot Arg (R140G of HspB1 and R120G of αB-crystallin) induced similar changes in the properties of two small heat shock proteins, whereas mutations of two neighboring residues (R140 and K141) induced different changes in the properties of HspB1.
journal_name
Biochimiejournal_title
Biochimieauthors
Nefedova VV,Datskevich PN,Sudnitsyna MV,Strelkov SV,Gusev NBdoi
10.1016/j.biochi.2013.04.014subject
Has Abstractpub_date
2013-08-01 00:00:00pages
1582-92issue
8eissn
0300-9084issn
1638-6183pii
S0300-9084(13)00132-6journal_volume
95pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Matrix metalloproteinase 9 (MMP-9) and interleukin-8 (IL-8) play major roles in tumor progression and invasion of breast cancer cells. The present study was undertaken to investigate the inhibitory mechanism of cell invasion by luteolin 8-C-β-fucopyranoside (named as LU8C-FP), a C-glycosylflavone, in human breast canc...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.07.021
更新日期：2013-11-01 00:00:00
abstract：:A methylene group of camphor is hydroxylated by a P450 monoxygenase system containing in addition to the cytochrome and flavoprotein dehydrogenase a two-iron, two-sulfur redox protein termed putidaredoxin. The regulatory control of electron transport equilibrium occurs via ligation of substrate and redoxin to the cyto...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80364-1
更新日期：1976-01-01 00:00:00
abstract：:Many natural phyto-products as perezone (Per) exhibit anti-cancer activities. Using experimental and computational studies, it was described that Poly ADP-ribose polymerase 1(PARP-1) inhibition and the induction of oxidative stress state explain the pro-apoptotic activity of Per. The aim of this study was to evaluate ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.03.003
更新日期：2020-01-01 00:00:00
abstract：:Catalase (E.C 1.11.1.6) was purified from leaves of Zandedeschia aethiopica to apparent homogeneity by a one-step hydrophobic interaction chromatography on a phenyl Sepharose CL-4B column. The purified enzyme preparation was obtained with a final recovery of enzyme activity of about 61% and a specific activity of 146 ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(88)90035-1
更新日期：1988-12-01 00:00:00
abstract：:Polynucleotide phosphorylase (PNPase, polyribonucleotide nucleotidyltransferase, EC 2.7.7.8) is a multifunctional protein, with a 3'-5' processive exoribonuclease, a Pi exchange, an RNA polymerase and an autoregulatory activity. The interaction between this enzyme and the mRNA target is crucial for its activities. In ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(02)01385-8
更新日期：2002-04-01 00:00:00
abstract：:This paper reviews recent data on the adenylate cyclase system of the yeast Saccharomyces cerevisiae. Since the discovery of yeast adenylate cyclase mutants and the possibility of molecular biological analysis, adenylate cyclase and the subsequent steps in the cAMP cascade have become subject of intense investigation....
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(85)80228-5
更新日期：1985-01-01 00:00:00
abstract：:Directed mutagenesis has been used to study the nicotinamide subsite of the glycolytic NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Residue Asn313 is involved together with the carboxyamide moiety of the nicotinamide ring in a complex network of hydrogen bonding interactions which fix the position o...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(90)90119-2
更新日期：1990-08-01 00:00:00
abstract：:Hemorrhage is the most potent manifestation of envenomation by Vipera ammodytes ammodytes (V. a. ammodytes) venom in man. A detailed description of the venom components contributing to this effect is thus medically very important. We have characterized a novel component, termed here VaH3, as a potently hemorrhagic sna...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.01.003
更新日期：2013-06-01 00:00:00
abstract：:Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and e...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.07.025
更新日期：2013-11-01 00:00:00
abstract：:When colonizing the digestive tract of mono-associated rats, Ruminococcus gnavus E1 - a bacterium isolated from human faeces - produced a trypsin-dependent anti-Clostridium perfringens substance collectively named Ruminococcin C (RumC). RumC was isolated from the caecal contents of E1-monocontaminated rats and found t...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.05.001
更新日期：2011-09-01 00:00:00
abstract：:The inhibition rates and spectral characteristics of 2 probes specific for the active-site serine residue of proteases were examined for evidence of conformational change of the proteases upon their binding to alpha 2-macroglobulin (alpha 2M). Elastase, chymotrypsin, trypsin, and plasmin were reacted with (7-nitrobenz...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(89)90004-7
更新日期：1989-03-01 00:00:00
abstract：:Epithelial Ca(2+)-regulation, which governs cornified envelope formation in the skin epidermis and hair follicles, closely coincides with the expression of S100A3, filaggrin and trichohyalin, and the post-translational modification of these proteins by Ca(2+)-dependent peptidylarginine deiminases. This review summariz...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2011.05.028
更新日期：2011-12-01 00:00:00
abstract：:The translation factor EF-P is required for efficient prokaryotic peptide bond synthesis on 70S ribosomes from fMet-tRNAfMet. This protein has been purified from Escherichia coli cells and the gene, efp, encoding it has been cloned and sequenced. We have isolated recombinant clones which overexpress a protein that co-...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(97)87619-5
更新日期：1997-01-01 00:00:00
abstract：:Intracellular cytoplasmic peptidoglycan (PG) intermediate levels were determined in Staphylococcus aureus during log-phase growth in enriched media. Levels of UDP-linked intermediates were quantitatively determined using ion pairing LC-MS/MS in negative mode, and amine intermediates were quantitatively determined ster...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2015.11.017
更新日期：2016-02-01 00:00:00
abstract：:Human Rad51 (HsRad51), a key element of the homologous recombination repair pathway, is related to the resistance of cancer cells to chemo- and radio-therapies. This protein is thus a good target for the development of anti-cancer treatments. We have searched for new inhibitors directed against HsRad51 using the Syste...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.08.006
更新日期：2010-12-01 00:00:00
abstract：:The emergence of drug-resistant Mycobacterium tuberculosis (Mtb) stains has escalated the need for developing more efficient drugs and therapeutic strategies against tuberculosis. Here we functionally annotate a secretory mycobacterial asparaginase Rv1538c (MtA) and describe its biochemical properties. MtA primarily e...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.12.023
更新日期：2021-01-05 00:00:00
abstract：:We present an approach for designing a knowledge-based system, called Sequence Acquisition In Context (SAIC), that will be able to cooperate with a biologist in the analysis of DNA sequences. The main task of the system is the acquisition of the expert knowledge that the biologist uses for solving ambiguities from gel...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(93)90167-q
更新日期：1993-01-01 00:00:00
abstract：:The family of interferon regulatory transcription factors (IRF) participates in the virus-induced and dsRNA-stimulated transcriptional regulation of either type I IFN genes or a definite set of genes which can also be activated by IFN. In this review, we place emphasis on the role of IRF-3 that associates with the coa...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(99)80021-2
更新日期：1998-08-01 00:00:00
abstract：:The dissociation of bound polyribosomes from ewe lactating mammary gland, by EDTA or Puromycin-KC1 treatment releases Messenger Ribonucleoprotein particles (mRNP'S) which sediment in sucrose gradients as a single component having a sedimentation coefficient of about 22S. RNAs derived from mRNPs sediment between 9S and...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80439-7
更新日期：1976-01-01 00:00:00
abstract：:In vertebrates, mannose 6-phosphate receptors [MPR300 (Mr 300 kDa) and MPR46 (Mr 46 kDa)] are highly conserved transmembrane glycoproteins that mediate transport of lysosomal enzymes to lysosomes. Our studies have revealed the appearance of these putative receptors in invertebrates such as the molluscs and deuterostom...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.02.007
更新日期：2012-05-01 00:00:00
abstract：:Isolation and properties of B. subtilis ts 19 mutant, isolated as thermosensitive for sporulation, are described. At the non permissive temperature (42degreesC), the mutant cells are blocked at stage zero of sporulation and do not excrete extracellular enzymes such as serylprotease and esterase. At the permissive temp...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80361-6
更新日期：1976-01-01 00:00:00
abstract：:Since the initial identification of leptin as the product of the ob gene in 1994, the signaling pathways by which this hormone alters cell physiology have been the subject of extensive investigations. The fact that leptin can induce nitric oxide (NO) production was first demonstrated in studies of the pituitary gland ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2012.06.017
更新日期：2012-10-01 00:00:00
abstract：:Snake venom phospholipases A2 (PLA2) share high sequence identities and a conserved structural scaffold, but show important functional differences. Only a few PLA2s have been purified and characterized from coral snake (Micrurus spp.) venoms, and their role in envenomation remains largely unknown. In this report, we d...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.03.008
更新日期：2017-06-01 00:00:00
abstract：:Phospholipases A(2) (PLA(2)s) and its generation of second messengers play an important role in signal transduction, cell proliferation, cell survival and gene expression. At low concentrations mediators of PLA(2) activity have a variety of physiological effects whereas high levels of PLA(2) and its metabolites are ge...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2010.04.007
更新日期：2010-06-01 00:00:00
abstract：:When ribosomes encounter mRNAs lacking stop codons, two quality-control machineries, NSD for nonstop mRNA decay and ribosome quality control (RQC) for co-translational degradation of the nonstop protein by the proteasome, are triggered to eliminate aberrant molecules. In yeast, it is known that Dom34 (a homolog of eRF...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.08.001
更新日期：2014-11-01 00:00:00
abstract：UNLABELLED:Pancreatic cancer (PC) is one of the most lethal human malignancies and a major health problem. Patients diagnosed with PC and treated with conventional approaches have an overall 5-year survival rate of less than 5%. Novel strategies are needed to treat this disease. Herein, we propose a combinatorial strat...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2015.07.012
更新日期：2015-09-01 00:00:00
abstract：:Direct demonstration of the reversible dissociation of native dimeric methionyl-tRNA synthetase from E. coli has been obtained using small angle neutron scattering and deuterated enzyme. Structural parameters of the fully deuterated dimer are very similar to the hydrogenated one. Analysis of the variations of the inte...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80205-4
更新日期：1985-06-01 00:00:00
abstract：:Bacterial superantigens (SAg) are the most potent activators of human T lymphocytes and recombinant immunotoxin using bacterial SAg shows promising clinical values. To engineer superantigen for immunotherapy of hepatocellular carcinoma, we genetically fused the superantigen staphylococcus enterotoxin A (SEA(D(227)A)) ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.04.005
更新日期：2005-08-01 00:00:00
abstract：:In septic shock patients, alterations of plasma phospholipid fatty acid profile have never been described. The purpose of this monocentric, non-interventional, observational prospective study was to describe this fatty acid profile in the early phase of septic shock in intensive care unit. Thirty-seven adult patients ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.08.006
更新日期：2013-11-01 00:00:00
abstract：:The present review deals with the place of single chain oligonucleotide ligands (aptamers) in affinity chromatography applied to proteins. Aptamers are not the only affinity ligands available but they represent an emerging and highly promising route that advantageously competes with antibodies in immunopurification pr...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2017.10.008
更新日期：2018-02-01 00:00:00
