Distinct functional properties of secretory l-asparaginase Rv1538c involved in phagosomal survival of Mycobacterium tuberculosis.

abstract：:An immunological demonstration of alpha-fetoprotein (AFP) in uterine cytosol from immature rats is reported. The identity between serum AFP and the estrogen binding 4.5 S macromolecular complex of uterine cytosol was demonstrated by the use of a specific immunoadsorbent to AFP. Analysis of the sedimentation profile ...

journal_title：Biochimie

authors： Aussel C,Uriel J,Michel G,Baulieu EE

更新日期：1974-01-01 00:00:00

abstract：:The termination of protein synthesis in bacteria requires codon-specific polypeptide release factors RF-1 (UAG/UAA specific) and RF-2 (UGA/UAA specific). We have proposed that release factors mimic tRNA and recognize the stop codon for polypeptide release (Nakamura et al (1996) Cell 87, 147-150). In contrast to the te...

journal_title：Biochimie

authors： Uno M,Ito K,Nakamura Y

更新日期：1996-01-01 00:00:00

abstract：:A reliable phylogenetic reconstruction of the evolutionary history of contemporary species depends on a robust identification of the universal common ancestor (UCA) at the root of the Tree of Life (ToL). That root polarizes the tree so that the evolutionary succession of ancestors to descendants is discernable. In eff...

journal_title：Biochimie

authors： Harish A,Kurland CG

更新日期：2017-07-01 00:00:00

abstract：:Plasmalogens (Pls) represent a specific subclass of glycerophospholipids characterized by the presence of a vinyl-ether bond at the sn-1 position of glycerol. Pls are quantitatively important in membranes of neuronal tissues, including the brain and the retina, where they can represent until almost two-third of ethano...

journal_title：Biochimie

authors： Saab S,Mazzocco J,Creuzot-Garcher CP,Bron AM,Bretillon L,Acar N

更新日期：2014-12-01 00:00:00

abstract：:Glucose-6 phosphatase (G6Pase), a key enzyme of glucose homeostasis, catalyses the hydrolysis of glucose-6 phosphate (G6P) to glucose and inorganic phosphate. A deficiency in G6Pase activity causes type 1 glycogen storage disease (GSD-1), mainly characterised by hypoglycaemia. Genetic analyses of the two forms of this...

journal_title：Biochimie

authors： Soty M,Chilloux J,Casteras S,Grichine A,Mithieux G,Gautier-Stein A

更新日期：2012-03-01 00:00:00

abstract：:The suhB gene of Escherichia coli has been defined by its mutant allele that suppresses other mutants in secY, rpoH, dnaB, and era. The suhB mutant by itself is cold sensitive, and is shown to have defects in protein synthesis. Starting with the suhB cold-sensitive mutant, cold-resistant suppressors were isolated. The...

journal_title：Biochimie

authors： Inada T,Nakamura Y

更新日期：1995-01-01 00:00:00

abstract：:Posttranscriptional gene regulation by small RNAs and its crucial impact on development, apoptosis, stem cell self-renewal and differentiation gained tremendous scientific attention since the discovery of RNA interference (RNAi) and microRNAs (miRNAs). However, in the last few years, many more examples for regulatory ...

journal_title：Biochimie

authors： Röther S,Meister G

更新日期：2011-11-01 00:00:00

abstract：:The periplasmic histidine permease of Salmonella typhimurium is composed of a soluble histidine-binding protein and three membrane-bound components. These latter are produced in very small amounts and only two, the Q and the P protein, have been previously identified. This paper describes the construction of a plasmid...

journal_title：Biochimie

authors： Ames GF,Nikaido K,Hobson A,Malcolm B

更新日期：1985-01-01 00:00:00

abstract：:A variety of species of GPDH undergo acylation at two of the four active cystein sites per mole of tetrameric enzyme. This reaction requires tightly bound NAD+, a situation restricted to two of the four NAD sites per tetramer. S leads to N acyl transfer from cysteins to lysine in the diacyl enzyme yields an inactive e...

journal_title：Biochimie

authors： Malhotra OP,Bernhard SA,Seydoux F

更新日期：1981-02-01 00:00:00

abstract：:We are reporting here an analysis of the chymotryptic peptides obtained from the tryptic heme-binding fragment of flavocytochrome b2 (cytochrome b2 core). These results completely establish the sequence of the 96 residue-long fragment, for which preliminary evidence has been published before [24]. We also report full ...

journal_title：Biochimie

authors： Guiard B,Lederer F

更新日期：1976-01-01 00:00:00

abstract：:SR-PSOX can function as a scavenger receptor, a chemokine and an adhesion molecule, and it could be an interesting player in the formation of atherosclerotic lesions. Our previous studies demonstrated that basic amino acid residues in the chemokine domain of SR-PSOX are critical for its functions. In this study the co...

journal_title：Biochimie

authors： Liu W,Yin L,Dai Y

更新日期：2013-02-01 00:00:00

abstract：:P53 is a transcription factor also called the "cellular gatekeeper of genome" because it can induce cell cycle arrest in G1, apoptosis or affect DNA replication in response to DNA damage. Wild type p53 is localised in both the cytoplasm and nucleus of first trimester trophoblastic cells (CTB). Immunoblotting of CTB wi...

journal_title：Biochimie

authors： Cohen M,Wuillemin C,Bischof P

更新日期：2008-06-01 00:00:00

abstract：:Caveolin is the protein marker of caveola-mediated endocytosis. Previously, we demonstrated by immunoblotting and immunofluorescence that an anti-chick embryo caveolin-1 monoclonal antibody (mAb) recognizes a protein in amoeba extracts. Nevertheless, the caveolin-1 gene is absent in the Entamoeba histolytica genome da...

journal_title：Biochimie

authors： Piña-Vázquez C,Reyes-López M,Mendoza-Hernández G,Bermúdez-Cruz RM,de la Garza M

更新日期：2014-12-01 00:00:00

abstract：:Insects have developed an efficient host defense against microorganisms, which involves humoral and cellular mechanisms. Numerous data highlight similarities between defense responses of insects and innate immunity of mammals. The fruit fly, Drosophila melanogaster, is a favorable model system for the analysis of the ...

journal_title：Biochimie

authors： Levy F,Rabel D,Charlet M,Bulet P,Hoffmann JA,Ehret-Sabatier L

更新日期：2004-09-01 00:00:00

abstract：:L-aromatic aminoacid decarboxylase has been purified more than thousand times from homogenates of rat brain, in several steps : centrifugation, DEAE-cellulose, CM cellulose, hydroxylapatite, DEAE sephadex. Its properties have been studied, most of them on an intermediate fraction of the purification, because of the in...

journal_title：Biochimie

authors： Corgier M,Pacheco H

更新日期：1975-01-01 00:00:00

abstract：:We previously reported that the rRNA methyltransferases RsmI and RsmH, which are responsible for cytidine dimethylation at position 1402 of 16S rRNA in the decoding center of the ribosome, contribute to Staphylococcus aureus virulence. Here we evaluated other 16S rRNA methyltransferases, including KsgA (RsmA), RsmB/F,...

journal_title：Biochimie

authors： Kyuma T,Kizaki H,Ryuno H,Sekimizu K,Kaito C

更新日期：2015-12-01 00:00:00

abstract：:DNA mismatch repair (MMR) is an important replication error avoidance mechanism that prevents mutation. The association of defective MMR with familial and sporadic gastrointestinal and endometrial cancer has been acknowledged for some years. More recently, it has become apparent that MMR defects are common in acute my...

journal_title：Biochimie

authors： Karran P,Offman J,Bignami M

更新日期：2003-11-01 00:00:00

abstract：:Since 2012, the interest for TMEM165 increased due to its implication in a rare genetic human disease named TMEM165-CDG (Congenital Disorder(s) of Glycosylation). TMEM165 is a Golgi localized protein, highly conserved through evolution and belonging to the uncharacterized protein family 0016 (UPF0016). Although the pr...

journal_title：Biochimie

authors： Lebredonchel E,Houdou M,Potelle S,de Bettignies G,Schulz C,Krzewinski Recchi MA,Lupashin V,Legrand D,Klein A,Foulquier F

更新日期：2019-10-01 00:00:00

abstract：:A high level of subcellular compartmentalization is a hallmark of eukaryotic cells. This intricate internal organization was present already in the common ancestor of all extant eukaryotes, and the determination of the origins and early evolution of the different organelles remains largely elusive. Organellar proteome...

journal_title：Biochimie

authors： Gabaldón T,Pittis AA

更新日期：2015-12-01 00:00:00

abstract：:Phospholipases A2 (PLA2) are a family of enzymes that catalyze the hydrolysis of the sn-2 ester bond of glycerophospholipids liberating lysophospholipids and free fatty acids; important second messengers involved in atherogenesis. Plasma PAF-acetylhydrolase (PAF-AH) or Lp-PLA2 is a Ca(2+)-independent PLA2 which is pro...

journal_title：Biochimie

authors： Karabina SA,Gora S,Atout R,Ninio E

更新日期：2010-06-01 00:00:00

abstract：:Mitochondrial membranes are essential for the good functioning of the organelle. For instance, the inner mitochondrial membrane contains the oxidative phosphorylation system that permits ATP synthesis. Phospholipids environment and especially cardiolipin are crucial for the mitochondrial energy metabolism. Indeed, car...

journal_title：Biochimie

authors： Dumas JF,Peyta L,Couet C,Servais S

更新日期：2013-01-01 00:00:00

abstract：:Leptin is a cytokine secreted by the adipose tissue that is involved in the control of body weight. We previously showed that a point mutation (R105W) in leptin results in leptin deficiency, marked obesity and hypogonadism in humans adults. Expression in COS1 cells showed impaired secretion and intracellular accumulat...

journal_title：Biochimie

authors： Boute N,Zilberfarb V,Camoin L,Bonnafous S,Le Marchand-Brustel Y,Issad T

更新日期：2004-06-01 00:00:00

abstract：:The lysosomal aspartic protease cathepsin D (cath-D) is overexpressed and hyper-secreted by epithelial breast cancer cells. This protease is an independent marker of poor prognosis in breast cancer as it is correlated with the incidence of clinical metastasis. In normal cells, cath-D is localized in intracellular vesi...

journal_title：Biochimie

authors： Masson O,Bach AS,Derocq D,Prébois C,Laurent-Matha V,Pattingre S,Liaudet-Coopman E

更新日期：2010-11-01 00:00:00

abstract：:NDPK-A, NDPK-B and NDPK-D are three enzymes which belong to the NDPK group I isoforms and are not only involved in metabolism process but also in transcriptional regulation, DNA cleavage, histidine protein kinase activity and metastasis development. Those enzymes were reported to bind to membranes either in mitochondr...

journal_title：Biochimie

authors： Francois-Moutal L,Marcillat O,Granjon T

更新日期：2014-10-01 00:00:00

abstract：:The measurement and potential technological significance of in vivo missense errors are briefly reviewed. A recently developed approach is described in which reporter enzyme activity is generated by mistranslation of a gene coding for an inactive mutant form of the enzyme. Initial results obtained using the alpha subu...

journal_title：Biochimie

authors： Cornut B,Willson RC

更新日期：1991-12-01 00:00:00

abstract：:Human serum was submitted to a one step displacement-ligand exchange chromatography. Displacement removed serum albumin and part of gamma-globulins. Ligand exchange furnished an enriched heme-hemopexin fraction. An original, non denaturing human heme-hemopexin preparation is proposed. ...

journal_title：Biochimie

authors： Plancke Y,Dautrevaux M,Biserte G

更新日期：1978-01-01 00:00:00

abstract：:tmRNA (10Sa RNA, ssrA) acts to rescue stalled bacterial ribosomes while encoding a peptide tag added trans-translationally to the nascent peptide, targeting it for proteolysis. Ribosomal protein S1 is required for tmRNA binding to isolated and poly U-programmed ribosomes. Mobility assays on native gels indicate that t...

journal_title：Biochimie

authors： Bordeau V,Felden B

更新日期：2002-08-01 00:00:00

abstract：:TcrX/Y is one of the twelve two component system (TCS) present in Mycobacterium tuberculosis. We have investigated the TcrX/Y interaction by in silico studies, pull down assay, radioactive phosphotransfer, surface plasmon resonance as well as crosstalk analysis of TcrY with TcrA - a non-cognate response regulator. Seq...

journal_title：Biochimie

authors： Bhattacharya M,Biswas A,Das AK

更新日期：2010-03-01 00:00:00

abstract：:Nicotinate phosphoribosyltransferase (NaPRT, EC 2.4.2.11) catalyzes the conversion of nicotinate (Na) to nicotinate mononucleotide, the first reaction of the Preiss-Handler pathway for the biosynthesis of NAD(+). Even though NaPRT activity has been described to be responsible for the ability of Na to increase NAD(+) l...

journal_title：Biochimie

authors： Galassi L,Di Stefano M,Brunetti L,Orsomando G,Amici A,Ruggieri S,Magni G

更新日期：2012-02-01 00:00:00

abstract：:A HU-like protein (HBl) of Bifidobacterium longum was purified and characterized. HBl is heat-stable and acid-resistant, and has a molecular weight of about 9.1 kDa as estimated by its mobility on electrophoresis. HBl is intermediate in basicity (pI 9.8) between the HU-1 and HU-2 proteins of Escherichia coli, and is d...

journal_title：Biochimie

authors： Goshima N,Kano Y,Imamoto F

更新日期：1990-04-01 00:00:00