Lethal double-stranded RNA processing activity of ribonuclease III in the absence of suhB protein of Escherichia coli.


:The suhB gene of Escherichia coli has been defined by its mutant allele that suppresses other mutants in secY, rpoH, dnaB, and era. The suhB mutant by itself is cold sensitive, and is shown to have defects in protein synthesis. Starting with the suhB cold-sensitive mutant, cold-resistant suppressors were isolated. These suppressors mapped to the gene rnc encoding RNase III (a double-strand RNA-processing enzyme), and restored normal protein synthesis to the suhB mutants. Two known rnc mutations, rnc70 or rnc105, both defective in RNA cleavage activity, similarly restored growth of suhB. These rnc mutations did not alter the level of suhB expression. These results suggest that wild-type RNase III exerts a lethal effect on E coli upon depletion of SuhB at low temperatures. One explanation is to assume that the double-strand RNA-processing activity of RNase III itself is potentially lethal to E coli and the normal function of SuhB modulates the lethal action of RNase III.






Inada T,Nakamura Y




Has Abstract


1995-01-01 00:00:00














  • Human liver fatty acid binding protein (hFABP1) gene is regulated by liver-enriched transcription factors HNF3β and C/EBPα.

    abstract::The human liver fatty acid binding protein (hFABP1) participates in cellular long-chain fatty acid trafficking and regulation of lipid metabolism and changes in hFABP1 are associated with an increased risk for type 2 diabetes, cardiovascular disease (CVD), and metabolic syndromes. Gene regulation of hFABP1 is not full...


    pub_type: 杂志文章


    authors: Wu YL,Peng XE,Wang D,Chen WN,Lin X

    更新日期:2012-02-01 00:00:00

  • Mitochondrial dysfunction in CD47-mediated caspase-independent cell death: ROS production in the absence of cytochrome c and AIF release.

    abstract::Ligation of CD47 by its natural ligand thrombospondin (TSP), or cross-linking by CD47 antibodies, triggers caspase-independent cell death in normal and leukemic cells. This kind of cell death is characterised by the cytoplasmic events of apoptosis including externalisation of phosphatidylserines and mitochondria swell...


    pub_type: 杂志文章


    authors: Roué G,Bitton N,Yuste VJ,Montange T,Rubio M,Dessauge F,Delettre C,Merle-Béral H,Sarfati M,Susin SA

    更新日期:2003-08-01 00:00:00

  • GTP-dependent modification of a 21-kDa substrate with NAD+ in bovine brain soluble fraction is not ADP-ribosylation of small G-protein but tailing of tRNA.

    abstract::Labeling of 21-kDa material was observed when bovine brain soluble fraction was incubated with [adenylate-32P]NAD+ in the presence of GTP. The 21-kDa substrate, slightly smaller than C3 substrate in size, was labeled even without C3 exoenzyme. GTP could be replaced by nucleoside triphosphates other than ATP while ATP ...


    pub_type: 杂志文章


    authors: Badruzzaman M,Miyaoka T,Terashima M,Yamada K,Ohno T,Yamamori C,Tsuchiya M,Shimoyama M

    更新日期:1997-07-01 00:00:00

  • Escherichia coli DnaA protein--phospholipid interactions: in vitro and in vivo.

    abstract::DNA replication in Escherichia coli is controlled at the initiation stage, possibly by regulation of the essential activity of DnaA protein. The cellular membrane has long been hypothesized to be involved in chromosomal replication. Accumulating evidence, both in vitro and in vivo, that supports the importance of memb...


    pub_type: 杂志文章,评审


    authors: Crooke E

    更新日期:2001-01-01 00:00:00

  • Automatic identification of large collections of protein-coding or rRNA sequences.

    abstract::The number of available genomic sequences is growing very fast, due to the development of massive sequencing techniques. Sequence identification is needed and contributes to the assessment of gene and species evolutionary relationships. Automated bioinformatics tools are thus necessary to carry out these identificatio...


    pub_type: 杂志文章


    authors: Arigon AM,Perrière G,Gouy M

    更新日期:2008-04-01 00:00:00

  • Osmotic stress, a proinflammatory signal in Caco-2 cells.

    abstract::Hyper- (450 mOsm/l) and hypoosmotic exposure (150 mOsm/l) of Caco-2 cells, a human intestinal epithelial cell line, induced a twofold- and a fivefold increase in the production of IL-8, a constitutively expressed cytokine, respectively. This was observed both in the presence or in the absence of added proinflammatory ...


    pub_type: 杂志文章


    authors: Hubert A,Cauliez B,Chedeville A,Husson A,Lavoinne A

    更新日期:2004-08-01 00:00:00

  • Phosphatidylinositol (4,5)-bisphosphate-mediated pathophysiological effect of HIV-1 Tat protein.

    abstract::Human immunodeficiency virus (HIV)-infected cells actively release the transcriptional activator (Tat) viral protein that is required for efficient HIV gene transcription. Extracellular Tat is able to enter uninfected cells. We recently reported that internalized Tat escapes endosomes to reach the cytosol and is then ...


    pub_type: 杂志文章,评审


    authors: Beaumelle B,Tóth P,Malak OA,Chopard C,Loussouarn G,Vitale N

    更新日期:2017-10-01 00:00:00

  • Hormonal regulation of liver phosphoenolpyruvate carboxykinase and glucokinase gene expression at weaning in the rat.

    abstract::During the suckling period, the rats are fed continuously with milk, which is a high-fat low-carbohydrate diet (HF). At weaning, milk is progressively replaced by the rat's laboratory chow which is a high-carbohydrate low-fat diet (HCHO), and this is accompanied by large hormonal modifications: an increase in plasma i...


    pub_type: 杂志文章,评审


    authors: Girard J,Perdereau D,Narkewicz M,Coupé C,Ferré P,Decaux JF,Bossard P

    更新日期:1991-01-01 00:00:00

  • Regulation of CIRL-1 proteolysis and trafficking.

    abstract::Calcium-independent receptor of alpha-latrotoxin (CIRL-1) is an adhesion G protein-coupled receptor implicated in the regulation of exocytosis. CIRL-1 biosynthesis involves constitutive proteolytic processing that takes place in the endoplasmic reticulum, requires the receptor's GPS domain, and yields heterologous two...


    pub_type: 杂志文章


    authors: Deyev IE,Petrenko AG

    更新日期:2010-04-01 00:00:00

  • Molecular evolution of thyroid peroxidase.

    abstract::Thyroid peroxidase is a member of a family of mammalian peroxidases that includes myeloperoxidase, lactoperoxidase, eosinophil peroxidase, and salivary peroxidase. Protein sequences showing a high degree of sequence similarity with mammalian peroxidases have recently been observed in several invertebrate species. A mu...


    pub_type: 杂志文章,评审


    authors: Taurog A

    更新日期:1999-05-01 00:00:00

  • c-Jun transcriptionally regulates alpha 1, 2-fucosyltransferase 1 (FUT1) in ovarian cancer.

    abstract::Alpha 1, 2-fucosyltransferase (FUT 1/2) is a rate-limiting enzyme that catalyzes the synthesis of Lewis y, a cell membrane-associated carbohydrate antigen. In human ovarian cancer, the upregulated expression of FUT1 and Lewis y is associated with advanced pathological stages and involved in cell proliferation, migrati...


    pub_type: 杂志文章


    authors: Gao N,Liu J,Liu D,Hao Y,Yan L,Ma Y,Zhuang H,Hu Z,Gao J,Yang Z,Shi H,Lin B

    更新日期:2014-12-01 00:00:00

  • DNA replication and indirect induction of the SOS response in Escherichia coli.

    abstract::The SOS response can be induced indirectly in Escherichia coli by infection with UV irradiated bacteriophage P1, lambda or M13. Induction, monitored quantitatively by means of a sfiA::lac operon fusion, was stronger with the plasmid phage P1 than with lambda, but the kinetics were similar, showing that plasmid and non...


    pub_type: 杂志文章


    authors: D'Ari R,Huisman O

    更新日期:1982-08-01 00:00:00

  • Characterization of the epitope for 4C4.1 mAb on alpha-latrotoxin using phage display-peptide libraries: prevention of toxin-dependent 45Ca(2+) uptake in non-neuronal human embryonic cells transiently expressing latrophilin.

    abstract::alpha-Latrotoxin, a protein toxin present in the venom of black widow spider, interacts with membrane receptors of neurons and other secretory cells to stimulate exocytosis. Two types of receptors have been identified and cloned. Our attention has been focused on the calcium independent receptor, a G-protein coupled r...


    pub_type: 杂志文章


    authors: Pescatori M,Grasso A

    更新日期:2000-09-01 00:00:00

  • Insights on calcium-independent phospholipid membrane damage by Lys49-PLA2 using tryptophan scanning mutagenesis of bothropstoxin-I from Bothrops jararacussu.

    abstract::Bothropstoxin-I (BthTx-I) is a homodimeric Lys49-PLA2 from the venom of the snake Bothrops jararacussu, which lacks hydrolytic activity against phospholipid substrates, yet permeabilizes membranes by a Ca2+-independent mechanism. The interaction of the BthTx-I with model membranes has been studied by intrinsic tryptop...


    pub_type: 杂志文章


    authors: Ferreira TL,Ruller R,Chioato L,Ward RJ

    更新日期:2008-09-01 00:00:00

  • Natural cysteine protease inhibitors in protozoa: Fifteen years of the chagasin family.

    abstract::Chagasin-type inhibitors comprise natural inhibitors of papain-like cysteine proteases that are distributed among Protist, Bacteria and Archaea. Chagasin was identified in the pathogenic protozoa Trypanosoma cruzi as an approximately 11 kDa protein that is a tight-binding and highly thermostable inhibitor of papain, c...


    pub_type: 杂志文章,评审


    authors: Costa TF,Lima AP

    更新日期:2016-03-01 00:00:00

  • Identification of the 30 kDa polypeptide in post mortem skeletal muscle as a degradation product of troponin-T.

    abstract::Although a 30 kDa polypeptide frequently is seen by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of post mortem (pm) skeletal muscle and in turn is used as an indicator of proteolysis, its origin has not been conclusively identified. We used antibodies to selected myofibrillar proteins...


    pub_type: 杂志文章


    authors: Ho CY,Stromer MH,Robson RM

    更新日期:1994-01-01 00:00:00

  • Deviant energetic metabolism of glycolytic cancer cells.

    abstract::The central glycolytic and oxidative pathways and the ATP-producing mechanisms differ in sane and malignant cells by their regulation and dynamics. Fast-growing, poorly-differentiated cancer cells characteristically show high aerobic glycolysis. In the same way, cholesterol biosynthesis, which occurs by normal pathway...


    pub_type: 杂志文章,评审


    authors: Baggetto LG

    更新日期:1992-11-01 00:00:00

  • Oxidative DNA damage processing in nuclear and mitochondrial DNA.

    abstract::Living organisms are constantly exposed to oxidative stress from environmental agents and from endogenous metabolic processes. The resulting oxidative modifications occur in proteins, lipids and DNA. Since proteins and lipids are readily degraded and resynthesized, the most significant consequence of the oxidative str...


    pub_type: 杂志文章,评审


    authors: Bohr VA,Dianov GL

    更新日期:1999-01-01 00:00:00

  • [In vivo metabolism of rat submaxillary gland phospholipids].

    abstract::The metabolism of the phospholipids of the submaxillary gland are studied after intraperitoneal injections of [32P]-orthophosphate in several groups of rats. The gland contains mainly phosphatidylcholines and phosphatidylethanolamines and in smaller quantities phosphatidylinositols, phosphatidylserines, sphingomyelins...


    pub_type: 杂志文章


    authors: Leclercq C,Di Costanzo G

    更新日期:1976-01-01 00:00:00

  • Induction of apoptotic cell death by DNA topoisomerase II inhibitors.

    abstract::We have analyzed the interference of antitumoral drugs acting through the inhibition of DNA topoisomerase II on the human HeLa cell metabolism. Different compounds characterized by a diverse mechanism of action have been used, namely m-amsacrine, an intercalative drug, etoposide, which does not intercalate DNA, and su...


    pub_type: 杂志文章


    authors: Negri C,Bernardi R,Donzelli M,Scovassi AI

    更新日期:1995-01-01 00:00:00

  • Activation of regulator ArcA in the presence of hypochlorite in Salmonella enterica serovar Typhimurium.

    abstract::Oxidative stress is the main mechanism behind efficient disinfectants, causing damage in bacterial macromolecules. Importantly, bacteria activate resistance mechanisms in response to damage generated by oxidative stress. Strategies allowing pathogens to survive oxidative stress are highly conserved among microorganism...


    pub_type: 杂志文章


    authors: Cabezas CE,Laulié AM,Briones AC,Pardo-Esté C,Lorca DE,Cofré AA,Morales EH,Mora AY,Krüger GI,Bueno SM,Hidalgo AA,Saavedra CP

    更新日期:2021-01-01 00:00:00

  • The glucomannan system from Aloe vahombe (liliaceae). III. Comparative studies on the glucomannan components isolated from the leaves.

    abstract::The polysaccharide mixture obtained by hot water extraction of Aloe vahombe leaves is composed of at least four different paritally acetylated glucomannans which differ in molecular weight, glucose to mannose ratios and acetyl contents. Furthermore, one fraction contains a small but significant amount of protein which...


    pub_type: 杂志文章


    authors: Vilkas E,Radjabi-Nassab F

    更新日期:1986-09-01 00:00:00

  • Pseudoknot and translational control in the expression of the S15 ribosomal protein.

    abstract::Translational autocontrol of the expression of the ribosomal protein S15 proceeds through the transitory formation of a pseudoknot. A synopsis of the known data is used to propose a molecular model of the mechanism involved and for the role of the pseudoknot. This latter structure is able to recruit 30S ribosomal subu...


    pub_type: 杂志文章


    authors: Bénard L,Philippe C,Ehresmann B,Ehresmann C,Portier C

    更新日期:1996-01-01 00:00:00

  • Attenuated mitochondrial NADP+-dependent isocitrate dehydrogenase activity induces apoptosis and hypertrophy of H9c2 cardiomyocytes.

    abstract::Oxidative stress, characterized by the accumulation of reactive oxygen species (ROS), is known to have numerous detrimental effects on the myocardium such as the induction of apoptotic cell death, hypertrophy, fibrosis, dysfunction, and dilatation. Over the past several years, we have shown that mitochondrial NADP(+)-...


    pub_type: 杂志文章


    authors: Lee JH,Park JW

    更新日期:2014-04-01 00:00:00

  • [Chicken erythrocyte nuclei: composition and analysis of phospholipids].

    abstract::While many works analyse lipid composition of cellular nuclei, essentially of liver, few describe bird erythrocyte nuclei. For them very discordant results are reported due to very different methods of preparation. In the method we adopted a good reproducibility is controlled by electron microscopy, evaluation of spec...


    pub_type: 杂志文章


    authors: Souillard C,Soula G

    更新日期:1976-01-01 00:00:00

  • An investigation into the ability of C-terminal homologues of Escherichia coli low molecular mass penicillin-binding proteins 4, 5 and 6 to undergo membrane interaction.

    abstract::The Escherichia coli low molecular mass penicillin-binding proteins (PBP4, PBP5 and PBP6) are a group of penicillin-sensitive enzymes involved in the final stages of cell wall assembly. It has been suggested that these proteins may interact with the periplasmic face of the inner membrane via C-terminal amphiphilic alp...


    pub_type: 杂志文章


    authors: Harris F,Phoenix DA

    更新日期:1997-04-01 00:00:00

  • A single element in the phosphoenolpyruvate carboxykinase gene mediates thiazolidinedione action specifically in adipocytes.

    abstract::Phosphoenolpyruvate carboxykinase (PEPCK) is the key enzyme in glyceroneogenesis, an important metabolic pathway that functions to restrain the release of non-esterified fatty acids (NEFAs) from adipocytes. The antidiabetic drugs known as thiazolidinediones (TZDs) are thought to achieve some of their benefits by lower...


    pub_type: 杂志文章


    authors: Glorian M,Duplus E,Beale EG,Scott DK,Granner DK,Forest C

    更新日期:2001-10-01 00:00:00

  • Identification of new bone morphogenetic protein-related members in invertebrates.

    abstract::Although believed to be widely distributed among the animal kingdom, transforming growth factor-beta (TGF-beta) superfamily members have mainly been characterized in vertebrate and in invertebrate model organisms such as Drosophila and C. elegans. To characterize such new factors in invertebrates, a PCR screen was per...


    pub_type: 杂志文章


    authors: Lelong C,Mathieu M,Favrel P

    更新日期:2001-05-01 00:00:00

  • Relationship between the inhibition of phosphatidic acid phosphohydrolase-1 by oleate and oleoyl-CoA ester and its apparent translocation.

    abstract::Phosphatidic acid phosphohydrolase-1 (PAP-1) activity is reversibly inhibited by fatty acids and their acyl-CoA esters and it appears paradoxical that these effectors have been reported to increase the liver's esterification capacity by translocating the rate-limiting enzyme PAP-1 from cytosol to the endoplasmic retic...


    pub_type: 杂志文章


    authors: Elabbadi N,Day CP,Gamouh A,Zyad A,Yeaman SJ

    更新日期:2005-05-01 00:00:00

  • Interferon-gamma is produced by another player of innate immune responses: the interferon-producing killer dendritic cell (IKDC).

    abstract::Interferon-gamma is a key cytokine in tumor immunosurveillance. The recently described interferon-producing killer dendritic cell (IKDC), can be distinguished from other innate effectors by its ability to kill a large variety of tumor cells and to produce high amounts of interferon-gamma after encountering tumors in t...


    pub_type: 杂志文章,评审


    authors: Bonmort M,Ullrich E,Mignot G,Jacobs B,Chaput N,Zitvogel L

    更新日期:2007-06-01 00:00:00