Overproduction of the membrane-bound components of the histidine permease from Salmonella typhimurium: identification of the M protein.

abstract：:All hormones and neurotransmitters which provoke their intracellular effects by increasing the cytosolic concentration of Ca2+ in their target cells also stimulate the breakdown of inositol phospholipids. Much evidence suggests that this breakdown is intimately involved in the mechanism which couples cell-surface rece...

journal_title：Biochimie

authors： Kirk CJ,Guillon G,Balestre MN,Creba JA,Michell RH,Jard S

更新日期：1985-10-01 00:00:00

abstract：:We have previously shown that withdrawal of folic acid led to metabolic reprogramming and a less aggressive phenotype in a mouse cell model of triple-negative breast cancer (TNBC). Herein, we evaluate the effects of folic acid withdrawal on transcriptomic profiles in these cells. Murine cell lines were originally deri...

journal_title：Biochimie

authors： Kok DE,O'Flanagan CH,Coleman MF,Ashkavand Z,Hursting SD,Krupenko SA

更新日期：2020-06-01 00:00:00

abstract：:The effects on intra- and extracellular pH of two polyenic derivatives of amphotericin B, N-fructosyl amphotericin B and N-fructosyl amphotericin B methyl-ester, were tested on HL-60 promyelocytic leukemia cells. Both derivatives raised the internal pH and reduced the external pH in weakly buffered medium. These resul...

journal_title：Biochimie

authors： Charretier E,Wietzerbin J

更新日期：1989-01-01 00:00:00

abstract：BACKGROUND:Fatty acids (FAs), and especially monounsaturated FAs (MUFAs) stimulate GLP-1 release. However, lipotoxicity is indicated in GLP-1 secreting cells following long-term exposure to elevated levels of saturated FAs (SFAs) in vivo and in vitro, where in vitro studies indicate that cosupplementation with MUFAs co...

journal_title：Biochimie

authors： Ntika S,Thombare K,Aryapoor M,Kristinsson H,Bergsten P,Krizhanovskii C

更新日期：2019-04-01 00:00:00

abstract：:We screened a cDNA library of the primitive eukaryote Dictyostelium discoideum constructed in the expression vector lambda gt11 with a specific antiserum directed against S-adenosyl-L-homocysteine hydrolase (AdoHcy hydrolase) and isolated cDNA clones coding for fusion proteins with beta-galactosidase. The identity of ...

journal_title：Biochimie

authors： Guitton MC,Part D,Veron M

更新日期：1988-06-01 00:00:00

abstract：:The study of modified nucleoside contributions to RNA chemistry, structure and function has been thwarted by the lack of a site-selected method of incorporation which is both versatile and adaptable to present synthetic technologies. A reproducible and versatile site-selected incorporation of nine differently modified...

journal_title：Biochimie

authors： Agris PF,Malkiewicz A,Kraszewski A,Everett K,Nawrot B,Sochacka E,Jankowska J,Guenther R

更新日期：1995-01-01 00:00:00

abstract：:The polysaccharide mixture obtained by hot water extraction of Aloe vahombe leaves is composed of at least four different paritally acetylated glucomannans which differ in molecular weight, glucose to mannose ratios and acetyl contents. Furthermore, one fraction contains a small but significant amount of protein which...

journal_title：Biochimie

authors： Vilkas E,Radjabi-Nassab F

更新日期：1986-09-01 00:00:00

abstract：:Immobilization of enzymes by crystallization and subsequent cross-linking provide structures characterized by a regular three-dimensional molecular arrangement and a high packing density. Compared to randomly immobilized enzymes, such structures permit more detailed analysis of the variations in kinetic properties ari...

journal_title：Biochimie

authors： Tüchsen E,Hvidt A,Ottesen M

更新日期：1980-01-01 00:00:00

abstract：:A synthetic phosphonate inhibitor designed for lipase inhibition but displaying a broader range of activity was covalently immobilized on a solid support to generate a function-directed tool targeting serine hydrolases. To achieve this goal, straightforward and reliable analytical techniques were developed, allowing t...

journal_title：Biochimie

authors： Delorme V,Raux B,Puppo R,Leclaire J,Cavalier JF,Marc S,Kamarajugadda PK,Buono G,Fotiadu F,Canaan S,Carrière F

更新日期：2014-12-01 00:00:00

abstract：:Phospholipases A(2) (PLA(2)s) and its generation of second messengers play an important role in signal transduction, cell proliferation, cell survival and gene expression. At low concentrations mediators of PLA(2) activity have a variety of physiological effects whereas high levels of PLA(2) and its metabolites are ge...

journal_title：Biochimie

authors： Wang J,Kolko M

更新日期：2010-06-01 00:00:00

abstract：:The thrombin-binding DNA aptamer (TBA) 5'-d(GGTTGGTGTGGTTGG)-3' forms a G-quadruplex that is necessary for binding to the coagulation factor thrombin. The stability of the G-quadruplex of TBA when bound to thrombin and potassium ion (K(+)) were investigated for the wild-type oligonucleotide and for mutants in which th...

journal_title：Biochimie

authors： Nagatoishi S,Isono N,Tsumoto K,Sugimoto N

更新日期：2011-08-01 00:00:00

abstract：:Calcium-independent receptor of alpha-latrotoxin (CIRL-1) is an adhesion G protein-coupled receptor implicated in the regulation of exocytosis. CIRL-1 biosynthesis involves constitutive proteolytic processing that takes place in the endoplasmic reticulum, requires the receptor's GPS domain, and yields heterologous two...

journal_title：Biochimie

authors： Deyev IE,Petrenko AG

更新日期：2010-04-01 00:00:00

abstract：:Administration of beta-adrenergic agonists to domestic species can lead to skeletal muscle hypertrophy, probably by reducing the rate of myofibrillar protein breakdown. Myofibrillar breakdown is associated with the calcium-dependent proteinase system (calpains I,II and calpastatin) whose activity also changes during b...

journal_title：Biochimie

authors： Bardsley RG,Allcock SM,Dawson JM,Dumelow NW,Higgins JA,Lasslett YV,Lockley AK,Parr T,Buttery PJ

更新日期：1992-03-01 00:00:00

abstract：:We have modified elongation factor Tu (EF-Tu) from Escherichia coli via mutagenesis of its encoding tufA gene to study its function-structure relationships. The isolation of the N-terminal half molecule of EF-Tu (G domain) has facilitated the analysis of the basic EF-Tu activities, since the G domain binds the substra...

journal_title：Biochimie

authors： Anborgh PH,Cool RH,Gümüsel F,Harmark K,Jacquet E,Weijland A,Mistou MY,Parmeggiani A

更新日期：1991-07-01 00:00:00

abstract：:RNA editing alters genomically encoded cytidines to uridines posttranscriptionally in higher plant mitochondria. Most of these editing events occur in translated regions and consequently alter the amino acid sequence. In Oenothera berteriana more than 500 editing sites have been detected and the total number of editin...

journal_title：Biochimie

authors： Yu W,Fester T,Bock H,Schuster W

更新日期：1995-01-01 00:00:00

abstract：:New strains of basidiomycetes producing extracellular laccases (Trametes ochracea 92-78, and Trametes hirsuta 56) have been found by screening of isolates of Trametes fungi. The laccases from T. hirsuta 56 and T. ochracea 92-78 as well as two laccases from previously found and described strains of basidiomycetes, name...

journal_title：Biochimie

authors： Shleev SV,Morozova OV,Nikitina OV,Gorshina ES,Rusinova TV,Serezhenkov VA,Burbaev DS,Gazaryan IG,Yaropolov AI

更新日期：2004-09-01 00:00:00

abstract：:1)Individual monosaccharides (uronic acids and aminosugars) have been purified following specific hydrolysis of the mucopolysaccharides from new born rat skin (hyaluronic acid, heparin + heparan sulfate, chondroitin sulfate A, B and C), after incubation with [U14C]-glucose under various conditions and for varying incu...

journal_title：Biochimie

authors： Hervé B,Clauser H

更新日期：1975-11-01 00:00:00

abstract：:Knowing the structure of a protein is essential to characterize its function and mechanism at the molecular level. Despite major advances in solving structures experimentally, most membrane protein native conformations remain unknown. This lack of available structures, along with the physical constraints imposed by th...

journal_title：Biochimie

authors： Postic G,Ghouzam Y,Gelly JC

更新日期：2015-08-01 00:00:00

abstract：:Complexes of purified 40S ribosomal subunits and initiation factor 3 from rabbit reticulocytes were crosslinked using the reversible protein crosslinking reagent, 2-iminothiolane, under conditions shown previously to lead to the formation of dimers between 40S proteins but not higher multimers. The activity of both th...

journal_title：Biochimie

authors： Tolan DR,Hershey JW,Traut RT

更新日期：1983-07-01 00:00:00

abstract：:The term 'RNA editing' was used for the first time in 1986 to describe the process of uridylate insertion into trypanosomal mitochondrial transcripts. Since then, the term has been used more generally to describe a large variety of processes involving base insertions, deletions and conversions that generate RNAs with ...

journal_title：Biochimie

authors： Blanc V,Jordana X,Litvak S,Araya A

更新日期：1996-01-01 00:00:00

abstract：:The effect of adenosine was tested on the energetic metabolism of fed rat liver cells after isolation. The cells were incubated in a buffered saline medium with glucose (5 mM) and adenosine (1 mM) for 30 minutes at 37 degrees C. This increased the concentration of the adenylic nucleotides ATP (+57 per cent, ADP (+39 p...

journal_title：Biochimie

authors： Marchand JC,Lavoinne A,Giroz M,Matray F

更新日期：1979-01-01 00:00:00

abstract：:Bacteria of the Lactococcus (formerly lactic Streptococcus) genus are described to emphasize certain less well-known facts of importance in their successful use as dairy starters. Other streptococci (non-rod forms) are briefly characterized to emphasize their usefulness in industrial milk fermentations. ...

journal_title：Biochimie

authors： Sandine WE

更新日期：1988-04-01 00:00:00

abstract：:Surfactants carrying either a hydrocarbon or a fluorocarbon alkyl chain have been synthesized. The polar head was either tris(hydroxymethyl)acrylamidomethane (THAM), telomerized THAM, or a glycosylated THAM moiety. The aqueous solubility of some of these molecules was increased by oxidizing to a sulfoxide the thioethe...

journal_title：Biochimie

authors： Chabaud E,Barthélémy P,Mora N,Popot JL,Pucci B

更新日期：1998-05-01 00:00:00

abstract：:Tryptocidine C (TpcC, cyclo[D-Phe1-Pro2-Trp3-D-Trp4-Asn5-Gln6-Trp7-Val8-Orn9-Leu10]) is a broad-spectrum antimicrobial peptide in the tyrothricin complex produced by a soil bacterium, Brevibacillus parabrevis. Electrospray mass spectrometric studies reveal the oligomerisation of TpcC into dimers and higher oligomers, ...

journal_title：Biochimie

authors： Rautenbach M,Kumar V,Vosloo JA,Masoudi Y,van Wyk RJ,Stander MA

更新日期：2020-12-18 00:00:00

abstract：:Stereospecific hydrogenations according to the general scheme [formula: see text] are of interest from a preparative and mechanistic point of view. Proteus mirabilis is suitable for the hydrogenation of a-keto-acids to R-hydroxy-acids, and a Clostridium strain for the reduction of enoates. Both have been immobilized i...

journal_title：Biochimie

authors： Tischer W,Tiemeyer W,Simon H

更新日期：1980-01-01 00:00:00

abstract：:In the bacterium Escherichia coli, H-NS-(H1, H1a) is a heat-stable protein with a molecular mass of 15.5 kDa involved in nucleoid organisation and gene regulation linked to certain signal transduction pathways. We have shown that, following addition of preparations of everted inner membrane vesicles, heat-stable cleav...

journal_title：Biochimie

authors： Goldberg MD,Canvin JR,Freestone P,Andersen C,Laoudj D,Williams PH,Holland IB,Norris V

更新日期：1997-06-01 00:00:00

abstract：:Explants from 7, 8, 9, 11, 13-day chick embryonic skin incorporating (3H) Uridine for different periods 1 hr, 3 or 4 hr and a chase with actinomycin) are studied with respect to free (F) or membrane bound (B) cytoplasmic polysomes and to RNA extracted from them. Polysome specific activity decreases at older stages but...

journal_title：Biochimie

authors： Desveaux-Chabrol J

更新日期：1976-01-01 00:00:00

abstract：:The eosinophil cationic protein (ECP) is a human antimicrobial protein involved in the host immune defense that belongs to the pancreatic RNase A family. ECP displays a wide range of antipathogen activities. The protein is highly cationic and its bactericidal activity is dependant on both cationic and hydrophobic surf...

journal_title：Biochimie

authors： Sánchez D,Moussaoui M,Carreras E,Torrent M,Nogués V,Boix E

更新日期：2011-02-01 00:00:00

abstract：:Experimental evidence accumulated to date by several research groups indicates that antisense oligodeoxynucleotides targeted against messenger RNA (mRNA) sequences located downstream of the initiation codon fail to inhibit the translation of this mRNA unless the hybrid is cleaved by RNase H. It has previously been sho...

journal_title：Biochimie

authors： Cazenave C,Frank P,Büsen W

更新日期：1993-01-01 00:00:00

abstract：:Histidine-proline-rich glycoprotein (HPRG), or histidine-rich glycoprotein (HRG), is a serum protein that is synthesized in the liver and is actively internalised by different cells, including skeletal muscle. The multidomain arrangement of HPRG comprises two modules at the N-terminus that are homologous to cystatin b...

journal_title：Biochimie

authors： Ronca F,Raggi A

更新日期：2015-11-01 00:00:00