Abstract：
:The effects on intra- and extracellular pH of two polyenic derivatives of amphotericin B, N-fructosyl amphotericin B and N-fructosyl amphotericin B methyl-ester, were tested on HL-60 promyelocytic leukemia cells. Both derivatives raised the internal pH and reduced the external pH in weakly buffered medium. These results support the idea that both derivatives induce outward proton movement from the cell to the external solution. In this respect, the non-esterified derivative proved to be more powerful that the esterified one. Under the present conditions, there was little or no regulation of pH in HL-60 cells, which exhibited an almost constant pH gradient between the external and internal pH (acid inside relative to outside). This deficiency in pH homeostasis might be due to the immature state of the HL-60 cells.
journal_name
Biochimiejournal_title
Biochimieauthors
Charretier E,Wietzerbin Jdoi
10.1016/0300-9084(89)90133-8subject
Has Abstractpub_date
1989-01-01 00:00:00pages
67-70issue
1eissn
0300-9084issn
1638-6183pii
0300-9084(89)90133-8journal_volume
71pub_type
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