Preferential incorporation of an exogenous cytokinin, N6-benzyladenine, into 18S and 25S ribosomal RNA of tobacco cells in suspension culture.

abstract：:The interactions of DNA with histone H4 and with its fragments N-H4 (1-84) and C-H4 (85-102) have been studied by using electrooptical techniques, viscosity and electron microscopy. Electron microscopy reveals that histone H4 induces a large folding of DNA molecules : this is in agreement with electrooptical measureme...

journal_title：Biochimie

authors： Palau AO,Mozo A,Querol E

更新日期：1980-01-01 00:00:00

abstract：:The antiviral activity of L-nucleoside analogs depends in part on the enantioselectivity of nucleoside kinases which catalyse their monophosphorylation. The substrate properties of human recombinant deoxycytidine kinase (dCK) and human recombinant deoxyguanosine kinase (dGK) with respect to L-adenosine and L-guanosine...

journal_title：Biochimie

authors： Gaubert G,Gosselin G,Boudou V,Imbach JL,Eriksson S,Maury G

更新日期：1999-11-01 00:00:00

abstract：:RNA phosphodiester bonds can be cleaved by metal ions, of which Pb2+ is one of the most effective. It can cleave both generally and site-specifically, depending on the substrate and the conditions. In addition, metal ions are also known to cleave ester bonds between amino acid and the 3'-end of transfer RNA. Here we r...

journal_title：Biochimie

authors： Otzen DE,Barciszewski J,Clark BF

更新日期：1994-01-01 00:00:00

abstract：:Porcine pancreatic extracts (PPE), also named pancreatin, are commonly used as a global source of pancreatic enzymes for enzyme replacement therapy in patients with exocrine pancreatic insufficiency. They are considered as a good substitute of human pancreatic enzymes and they have become a material of choice for in v...

journal_title：Biochimie

authors： Salhi A,Amara S,Mansuelle P,Puppo R,Lebrun R,Gontero B,Aloulou A,Carrière F

更新日期：2020-02-01 00:00:00

abstract：:Adipose tissue is an endocrine organ that plays an essential role in regulating several metabolic functions through the secretion of biological mediators called "adipokines". Dysregulation of adipokines plays a crucial role in obesity-related diseases. Adiponectin (APN) is the most abundant adipokine accounting for th...

journal_title：Biochimie

authors： Villarreal-Molina MT,Antuna-Puente B

更新日期：2012-10-01 00:00:00

abstract：:By use of procollagen proline hydroxylas which catalyses the substitution of the hydrogen in the trans position on carbon 4 with an hydroxyl on L-proline residues of procollagen, we have shown that the L-[3H-4] proline product of C.E.N. Saclay contained 29 per cent of the trans and 71 per cent of the cis form. It was ...

journal_title：Biochimie

authors： Farjanel J,Pichat L,Audinot M,Perier C,Frey J

更新日期：1976-01-01 00:00:00

abstract：:Lysosomal cysteine proteinases were fractionated from partially purified rat muscle lysosomes. By gel filtration on Sephadex G75, cathepsin D was separated from two thiol-requiring proteolytic fractions of Mr 25 000 and 55 000, respectively. By chromatofocusing, the first fraction (Mr = 25 000) was resolved into three...

journal_title：Biochimie

authors： Obled A,Ouali A,Valin C

更新日期：1984-09-01 00:00:00

abstract：:Sialate 9(4)-O-acetylesterases (EC 3.1.1.53) have been isolated from equine liver, bovine brain and influenza C virus. In this latter case, the esterase represents the receptor-destroying enzyme of the virus. The kinetic properties of these enzymes were determined with Neu5,9Ac2 and in part with 4-methylumbelliferyl a...

journal_title：Biochimie

authors： Schauer R,Reuter G,Stoll S

更新日期：1988-11-01 00:00:00

abstract：PURPOSE:We evaluated the clinical relevance of microRNA-149 (miR-149) in neuroblastoma (NB) and its functional roles in regulating NB proliferation in vitro. METHODS:QRT-PCR was used to evaluate miR-149 expression in NB cell lines and primary NB tumors. Association between endogenous miR-149 expression in primary NB t...

journal_title：Biochimie

authors： Xu Y,Chen X,Lin L,Chen H,Yu S,Li D

更新日期：2017-08-01 00:00:00

abstract：:Calprotectin (CP) is an abundant protein in human neutrophilic granulocytes and macrophages. In humans, serum, urine, and fecal concentrations of neutrophil-derived proteins, such as CP are used as markers of disease activity for conditions associated with increased neutrophil activity, such as inflammatory bowel dise...

journal_title：Biochimie

authors： Heilmann RM,Suchodolski JS,Steiner JM

更新日期：2008-09-01 00:00:00

abstract：:A novel antimicrobial peptide (AMP) was identified from the centipede Scolopendra subspinipes mutilans by RNA sequencing, and the amino acid sequences predicted from the sequenced mRNAs were compared with those of known AMPs. We named this peptide scolopendin, according to its origin, and investigated the molecular me...

journal_title：Biochimie

authors： Lee W,Hwang JS,Lee DG

更新日期：2015-11-01 00:00:00

abstract：:The short isoform of Rho guanine nucleotide exchange factor ARHGEF5 is known as TIM, which plays diverse roles in, for example, tumorigenesis, neuronal development and Src-induced podosome formation through the activation of its substrates, the Rho family of GTPases. The activation is auto-inhibited by a putative heli...

journal_title：Biochimie

authors： He P,Tan DL,Liu HX,Lv FL,Wu W

更新日期：2015-04-01 00:00:00

abstract：:We are here reporting that calpain-1 is a constitutive component of a distinct lipid raft/caveolin-1 microdomain isolated from bEnd5 cells in association with endothelial nitric oxide synthase (eNOS) and heat shock protein 90 (HSP90). Perturbations in intracellular calcium concentration by Ca2+-ionophore A23187 or pro...

journal_title：Biochimie

authors： Martines A,Stifanese R,Faelli EL,Perasso L,Melloni I,Ruggeri P,Averna M

更新日期：2017-02-01 00:00:00

abstract：:The dissociation of bound polyribosomes from ewe lactating mammary gland, by EDTA or Puromycin-KC1 treatment releases Messenger Ribonucleoprotein particles (mRNP'S) which sediment in sucrose gradients as a single component having a sedimentation coefficient of about 22S. RNAs derived from mRNPs sediment between 9S and...

journal_title：Biochimie

authors： Assairi L,Gaye P,Houdebine LM

更新日期：1976-01-01 00:00:00

abstract：:The published literature contains a number of examples of normally non-cytoplasmic proteins whose transport out of the cytoplasm is not completely abolished by drastic alterations to their routing signals (signal sequences, etc). Furthermore, there are numerous examples of cytoplasmic proteins that can be routed to an...

journal_title：Biochimie

authors： Pugsley AP

更新日期：1990-02-01 00:00:00

abstract：:Versican and decorin, two proteoglycans (PGs) with contradictory roles in the pathophysiology of cancer, comprise important stromal components in many tumor types and play a crucial role in the progression of cancer. In this study, we provide direct evidence for a significant and stage-related accumulation of versican...

journal_title：Biochimie

authors： Skandalis SS,Theocharis AD,Papageorgakopoulou N,Vynios DH,Theocharis DA

更新日期：2006-09-01 00:00:00

abstract：:Diabetic cardiomyopathy is related to oxidative stress and correlated with the presence of advanced glycation end products (AGEs). In a clinical setting, AGEs can be detected in patients presenting diabetic cardiomyopathy; however, the underlying mechanism has yet to be elucidated. In our previous study, AGEs increase...

journal_title：Biochimie

authors： Ko SY,Chang SS,Lin IH,Chen HI

更新日期：2015-11-01 00:00:00

abstract：:We have modified elongation factor Tu (EF-Tu) from Escherichia coli via mutagenesis of its encoding tufA gene to study its function-structure relationships. The isolation of the N-terminal half molecule of EF-Tu (G domain) has facilitated the analysis of the basic EF-Tu activities, since the G domain binds the substra...

journal_title：Biochimie

authors： Anborgh PH,Cool RH,Gümüsel F,Harmark K,Jacquet E,Weijland A,Mistou MY,Parmeggiani A

更新日期：1991-07-01 00:00:00

abstract：:Many human conditions, including neurological diseases, atherosclerosis, cancer, diabetic complications and aging, are thought to be associated with oxidative stress (OS). The development of reliable and informative markers for the characterization of OS in humans is thus highly important. Various endogenous markers a...

journal_title：Biochimie

authors： Vaya J

更新日期：2013-03-01 00:00:00

abstract：:Antibodies to Guo-AF were elicited in rabbits by immunization with bovine serum albumin -Guo-AF conjugate. The antibodies were purified by affinity chromatography. The affinity of the antibodies towards several ligands was studied by radioimmunoassays. The antibodies recognize Gua-AAF and Guo-AF (K(Guo-AF)/K(Guo-AAF) ...

journal_title：Biochimie

authors： Rio P,Leng M

更新日期：1980-01-01 00:00:00

abstract：:Several mechanistic hypotheses have been proposed for how carotenoid pigmentation of integumentary structures can serve as an honest signal of individual quality. These hypotheses are founded on proposed links between carotenoids, immuno responsiveness, and oxidative stress, but an absence of biochemical information o...

journal_title：Biochimie

authors： Johnson JD,Hill GE

更新日期：2013-02-01 00:00:00

abstract：:Labeling of 21-kDa material was observed when bovine brain soluble fraction was incubated with [adenylate-32P]NAD+ in the presence of GTP. The 21-kDa substrate, slightly smaller than C3 substrate in size, was labeled even without C3 exoenzyme. GTP could be replaced by nucleoside triphosphates other than ATP while ATP ...

journal_title：Biochimie

authors： Badruzzaman M,Miyaoka T,Terashima M,Yamada K,Ohno T,Yamamori C,Tsuchiya M,Shimoyama M

更新日期：1997-07-01 00:00:00

abstract：:Synthesis of the tripeptide Z-Phe-Arg-SerNH2 has been accomplished by a recombinant cysteine protease, cathepsin L1 from liver fluke (Fasciola hepatica), using Z-Phe-Arg-OMe as acyl acceptor and SerNH2 as nucleophile in 0.1 M ammonium acetate pH 9.0-12.5% v/v acetonitrile at 37 degrees C. LC-MS detection indicated tri...

journal_title：Biochimie

authors： Ruth DM,McMahon G,O'Fágáin C

更新日期：2006-01-01 00:00:00

abstract：:We have previously proposed that the rate of ribosome function during balanced growth in E. coli, expressed as the rate of peptide chain elongation, is adjusted by a feedback mechanism: whenever that rate is submaximal (i.e. below 22 amino acid residues polymerized per active ribosome at 37 degrees C), the feedback si...

journal_title：Biochimie

authors： Bremer H,Dennis P

更新日期：2008-03-01 00:00:00

abstract：:Clustered apurinic/apyrimidinic (AP) sites are more cytotoxic than isolated AP lesions because double strand breaks (DSB) can be formed during repair of closely positioned bistranded AP sites. Formation of DSB due to simultaneous cleavage of bistranded AP sites may be regulated by proteins specifically interacting wit...

journal_title：Biochimie

authors： Ilina ES,Khodyreva SN,Lavrik OI

更新日期：2018-07-01 00:00:00

abstract：:The maximum activities of some key enzymes of metabolism were studied in lungs of fed and 48-h-starved rats. The maximum activity of hexokinase in the lung is similar to that of other tissues of the body, but lower than that of phosphorylase and 6-phosphofructokinase. High activities of glucose-6-phosphate dehydrogena...

journal_title：Biochimie

authors： Ardawi MS

更新日期：1991-05-01 00:00:00

abstract：:Cysteine-rich secretory proteins (CRISPs), characterized by 16 conserved cysteines, are distributed in a wide range of organisms, such as secernenteas, amphibians, reptiles and mammals. In the previous studies, a novel CRISP family member (cysteine-rich buccal gland protein, CRBGP) was separated from the buccal gland ...

journal_title：Biochimie

authors： Jiang Q,Liu Y,Duan D,Gou M,Wang H,Wang J,Li Q,Xiao R

更新日期：2016-04-01 00:00:00

abstract：:L-aromatic aminoacid decarboxylase has been purified more than thousand times from homogenates of rat brain, in several steps : centrifugation, DEAE-cellulose, CM cellulose, hydroxylapatite, DEAE sephadex. Its properties have been studied, most of them on an intermediate fraction of the purification, because of the in...

journal_title：Biochimie

authors： Corgier M,Pacheco H

更新日期：1975-01-01 00:00:00

abstract：:The structure difference between the free operator of the lac system d(GCTCACAAT).d(ATTGTGAGC) and the same operator complexed to the headpiece of the lac repressor has been investigated by 2-D-1H NMR spectroscopy in conjunction with molecular modelling in internal coordinates (JUMNA). The free and complexed operator ...

journal_title：Biochimie

authors： Gincel E,Lancelot G,Maurizot JC,Thuong NT,Vovelle F

更新日期：1994-01-01 00:00:00

abstract：:Stroke is a worldwide major cause of mortality and morbidity. Preclinical studies have identified over 1000 molecules with brain-protective properties. More than 200 clinical trials have evaluated neuroprotective candidates for ischemic stroke yet, to date almost all failed, leading to a re-analysis of treatment strat...

journal_title：Biochimie

authors： Blondeau N

更新日期：2016-01-01 00:00:00