Dual hydrolytic role for Pb(II) ions.

Abstract:

:RNA phosphodiester bonds can be cleaved by metal ions, of which Pb2+ is one of the most effective. It can cleave both generally and site-specifically, depending on the substrate and the conditions. In addition, metal ions are also known to cleave ester bonds between amino acid and the 3'-end of transfer RNA. Here we report that in aminoacylated transfer RNA, Pb2+ ions cleave internucleotide bonds in the 3'-end of tRNA and also cleaves the bond between tRNA and its amino-acid, attached at the 3'-end via an ester bond to the terminal ribose in aminoacyl tRNA. The two reactions proceed at different rates. The rate of deacylation is significantly faster than the rate of cleavage of phosphodiester bonds, with a pH-optimum of 7. This dual hydrolytic role is not seen for other metal ions examined, namely Zn(II), Cd(II) and Mn(II). The rate of the two kinds of hydrolyses by Pb2+ ions is compared with that of other metal-ions. The mechanism of cleavage is investigated further by modification of the 3'-end of tRNA.

journal_name

Biochimie

journal_title

Biochimie

authors

Otzen DE,Barciszewski J,Clark BF

doi

10.1016/0300-9084(94)90058-2

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

15-21

issue

1

eissn

0300-9084

issn

1638-6183

pii

0300-9084(94)90058-2

journal_volume

76

pub_type

杂志文章
  • Light induced H2 evolution in a hydrogenase-TiO2 particle system by direct electron transfer or via rhodium complexes.

    abstract::Three different hydrogenases (isolated from Clostridium pasteurianum, Desulfovibrio desulfuricans strain Norway 4 and D. baculatus 9974) added to a suspension of TiO2 (anatase) powder are able to catalyze H2 evolution under band gap illumination of the semiconducting particles, and in the presence of EDTA or methanol ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(86)81086-0

    authors: Cuendet P,Rao KK,Grätzel M,Hall DO

    更新日期:1986-01-01 00:00:00

  • DNA binding thermodynamics and site stoichiometry as a function of polyamide size.

    abstract::The interactions of 6-8 ring hairpin polyamides (PAs) with the minor groove of DNA have been investigated extensively. More recent studies of large antiviral PAs (14-20 rings) active against small DNA tumor viruses lead to questions regarding the extent to which the DNA binding behaviors of the well studied, smaller P...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2019.07.021

    authors: Song Y,Niederschulte J,Bales KN,Park AH,Bashkin JK,Dupureur CM

    更新日期:2019-10-01 00:00:00

  • Redox properties and activity studies on a nickel-containing hydrogenase isolated from a halophilic sulfate reducer Desulfovibrio salexigens.

    abstract::A soluble hydrogenase from the halophilic sulfate reducing bacterium Desulfovibrio salexigens, strain British Guiana (NCIB 8403) has been purified to apparent homogeneity with a final specific activity of 760 mumoles H2 evolved/min/mg (an overall 180-fold purification with 20% recovery yield). The enzyme is composed o...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(86)81071-9

    authors: Teixeira M,Moura I,Fauque G,Czechowski M,Berlier Y,Lespinat PA,Le Gall J,Xavier AV,Moura JJ

    更新日期:1986-01-01 00:00:00

  • Molecular parameters involved in bee-plant relationships: a biological and chemical approach.

    abstract::Honeybee-plant relationships are based on a conditioning process in which olfactory (plant aroma) and gustatory cues (mainly nectars) are closely linked, leading to a selective foraging behaviour. Among crops dependent upon entomophilous cross-pollination, the sunflower has recently undergone extensive expansion due t...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(87)90186-6

    authors: Pham-Delegue MH,Etievant P,Masson C

    更新日期:1987-06-01 00:00:00

  • LPA-induced suppression of periostin in human osteosarcoma cells is mediated by the LPA(1)/Egr-1 axis.

    abstract::Lysophosphatidic acid (LPA), a naturally occurring bioactive phospholipid, mediates a multitude of (patho)physiological events including activation of mitogen-activated protein kinases (MAPKs). As LPA may induce cellular reponses in human osteosarcoma, the present study aimed at investigating expression of various LPA...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2012.05.023

    authors: Windischhofer W,Huber E,Rossmann C,Semlitsch M,Kitz K,Rauh A,Devaney T,Leis HJ,Malle E

    更新日期:2012-09-01 00:00:00

  • Impact of protein dynamics on secondary structure prediction.

    abstract::Protein 3D structures support their biological functions. As the number of protein structures is negligible in regards to the number of available protein sequences, prediction methodologies relying only on protein sequences are essential tools. In this field, protein secondary structure prediction (PSSPs) is a mature ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2020.09.006

    authors: de Brevern AG

    更新日期:2020-12-01 00:00:00

  • Is there NO help for leptin?

    abstract::Since the initial identification of leptin as the product of the ob gene in 1994, the signaling pathways by which this hormone alters cell physiology have been the subject of extensive investigations. The fact that leptin can induce nitric oxide (NO) production was first demonstrated in studies of the pituitary gland ...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2012.06.017

    authors: Joffin N,Niang F,Forest C,Jaubert AM

    更新日期:2012-10-01 00:00:00

  • Molecular sorting of proteins into the cisternal secretory pathway.

    abstract::Cotranslational translocation of exportable proteins across the RER membrane prior to their release into the extracellular space has been essentially described by use of canine pancreatic microsomal membranes. Intracisternal segregation of nascent secretory proteins was observed to be irreversible and proteolytic remo...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/0300-9084(88)90194-0

    authors: Scheele GA

    更新日期:1988-09-01 00:00:00

  • Relationship between the inhibition of phosphatidic acid phosphohydrolase-1 by oleate and oleoyl-CoA ester and its apparent translocation.

    abstract::Phosphatidic acid phosphohydrolase-1 (PAP-1) activity is reversibly inhibited by fatty acids and their acyl-CoA esters and it appears paradoxical that these effectors have been reported to increase the liver's esterification capacity by translocating the rate-limiting enzyme PAP-1 from cytosol to the endoplasmic retic...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2005.01.003

    authors: Elabbadi N,Day CP,Gamouh A,Zyad A,Yeaman SJ

    更新日期:2005-05-01 00:00:00

  • Perspectives with cryogenic RF probes in biomedical MRI.

    abstract::Since discovery of high-temperature superconductive (HTS) ceramics by Bednorz and Muller in 1986, there has been an accelerated development of cold technologies in industry, including the domain of NMR detection. The purpose of this paper is to fix ideas about the stage that cryogenic radio frequency (RF) probe techni...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2003.09.016

    authors: Darrasse L,Ginefri JC

    更新日期:2003-09-01 00:00:00

  • Poly(ADP-ribose) degradation by post-nuclear extracts from human cells.

    abstract::The nuclear metabolism of poly(ADP-ribose) is mainly regulated by poly(ADP-ribose) polymerase-1 (PARP-1) and by poly(ADP-ribose) glycohydrolase (PARG). A PARP-like enzyme, V-PARP, and a PARG isoform are present in the extra-nuclear compartment of mammalian cells, even if poly(ADP-ribose) has never been detected therei...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(02)00017-2

    authors: Rossi L,Denegri M,Torti M,Poirier GG,Ivana Scovassi A

    更新日期:2002-12-01 00:00:00

  • Feedback control of ribosome function in Escherichia coli.

    abstract::We have previously proposed that the rate of ribosome function during balanced growth in E. coli, expressed as the rate of peptide chain elongation, is adjusted by a feedback mechanism: whenever that rate is submaximal (i.e. below 22 amino acid residues polymerized per active ribosome at 37 degrees C), the feedback si...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2007.10.008

    authors: Bremer H,Dennis P

    更新日期:2008-03-01 00:00:00

  • [Energy-dependent translocation of amino-phospholipids in the erythrocyte membrane].

    abstract::In this review, we show how the stability of the asymmetric transverse distribution of phospholipids and the physiological role of the asymmetric distribution can be explained. Experiments with paramagnetic or fluorescent lipids enabled us to show that in fresh red blood cells, i.e. containing ATP, and in resealed gho...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/s0300-9084(86)80005-0

    authors: Devaux PF,Zachowski A,Favre E,Fellmann P,Cribier S,Geldwerth D,Hervé P,Seigneuret M

    更新日期:1986-03-01 00:00:00

  • Seipin deficiency alters fatty acid Delta9 desaturation and lipid droplet formation in Berardinelli-Seip congenital lipodystrophy.

    abstract::Berardinelli-Seip congenital lipodystrophy (BSCL) is a rare recessive disease characterized by near absence of adipose tissue and severe insulin resistance. In most cases, BSCL is due to loss-of-function mutations in the genes encoding either seipin of unknown function or 1-acyl-glycerol-3-phosphate O-acyltransferase ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2009.01.011

    authors: Boutet E,El Mourabit H,Prot M,Nemani M,Khallouf E,Colard O,Maurice M,Durand-Schneider AM,Chrétien Y,Grès S,Wolf C,Saulnier-Blache JS,Capeau J,Magré J

    更新日期:2009-06-01 00:00:00

  • Identification of ribosomal protein S1 as a poly(A) binding protein in Escherichia coli.

    abstract::To elucidate the metabolic function of mRNA polyadenylation in Escherichia coli. we searched for a polyadenylate-binding protein as a potential mediator of the function of the poly(A) moiety. Using a nitrocellulose filter-binding assay and a Northwestern blot technique, a protein in the ribosomal supernatant fraction ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(97)82741-1

    authors: Kalapos MP,Paulus H,Sarkar N

    更新日期:1997-09-01 00:00:00

  • Inhibition of farnesylation blocks growth but not differentiation in FRTL-5 thyroid cells.

    abstract::The cholesterol lowering drug lovastatin, a competitive inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, blocks DNA synthesis and proliferation of thyrotropin (TSH) primed FRTL-5 rat thyroid cells. The blockade can be completely prevented and/or reversed by mevalonate and largely prevented and/o...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(99)80072-8

    authors: Bifulco M,Laezza C,Aloj SM

    更新日期:1999-04-01 00:00:00

  • Design of a chemiluminescent and bioluminescent photometer.

    abstract::An apparatus for chemi- and bioluminescence measurements is described. The main features are in high sensitivity: for example, 5 10(-12) M adenosine triphosphate, 5 10(-7) M glucose, 2 10(-9) M perhydrol and 4 10(-13) M peroxidase are easily detected; its fast mixing time and the possibility of injecting a reactant, a...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(80)80061-7

    authors: Arrio B,Lecuyer B,Dupaix A,Volfin P,Jousset M,Carrette A

    更新日期:1980-01-01 00:00:00

  • Characteristics of beta-adrenergic receptors in isolated cells and in crude membranes of brown adipose tissue.

    abstract::In relation to decreased metabolic sensitivity to catecholamines observed, in vitro, in brown fat of cold-acclimated rats, beta-adrenergic receptors were studied in isolated cells and in a crude membrane preparation from rat interscapular brown adipose tissue. [3H] dihydroalprenolol binding had the same characteristic...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(84)90153-6

    authors: Senault C,Le Comte V,Portet R

    更新日期:1984-07-01 00:00:00

  • Pyruvate reverses metabolic effects produced by hypoxia in glioma and hepatoma cell cultures.

    abstract::The intervention of pyruvate in glucose metabolism was investigated during hypoxic stress in tumour cell cultures having respiratory capacities under normoxic conditions. Results obtained with nuclear magnetic resonance (NMR) spectroscopy showed that, under normoxic conditions, rat glioma C6 and human hepatoma Hep G2 ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(02)00008-1

    authors: Perrin A,Roudier E,Duborjal H,Bachelet C,Riva-Lavieille C,Leverve X,Massarelli R

    更新日期:2002-10-01 00:00:00

  • Site directed mutagenesis of StSUT1 reveals target amino acids of regulation and stability.

    abstract::Plant sucrose transporters (SUTs) are functional as sucrose-proton-cotransporters with an optimal transport activity in the acidic pH range. Recently, the pH optimum of the Solanum tuberosum sucrose transporter StSUT1 was experimentally determined to range at an unexpectedly low pH of 3 or even below. Various research...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2013.07.028

    authors: Krügel U,Wiederhold E,Pustogowa J,Hackel A,Grimm B,Kühn C

    更新日期:2013-11-01 00:00:00

  • The heart-protective mechanism of nitronyl nitroxide radicals on murine viral myocarditis induced by CVB3.

    abstract::Our previous researches showed that nitronyl nitroxyl derivatives, NNP and NNVP were good anti-oxidants and provided radioprotective effects in C6 cells. The objective of the present study is to investigate the possible antiviral effects and underlying pharmacological of the two nitronyl nitroxide radicals against CVB...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2012.05.015

    authors: Wang H,Gao P,Jing L,Qin X,Sun X

    更新日期:2012-09-01 00:00:00

  • Probing the active site of beta-lactamase R-TEM1 by informational suppression.

    abstract::Using a new extended set of 13 amber suppressors in E coli, systematic amino-acid replacements were performed at positions 104(E) and 238(G) of TEM-1 beta-lactamase from PUC19. The enzyme is tolerant to most substitutions tested at position 104. Missense revertants E104K, E104S or E104Y exhibited only minor changes in...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(90)90073-p

    authors: Lenfant F,Labia R,Masson JM

    更新日期:1990-06-01 00:00:00

  • One-step purification and properties of catalase from leaves of Zantedeschia aethiopica.

    abstract::Catalase (E.C 1.11.1.6) was purified from leaves of Zandedeschia aethiopica to apparent homogeneity by a one-step hydrophobic interaction chromatography on a phenyl Sepharose CL-4B column. The purified enzyme preparation was obtained with a final recovery of enzyme activity of about 61% and a specific activity of 146 ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(88)90035-1

    authors: Trindade H,Karmali A,Pais MS

    更新日期:1988-12-01 00:00:00

  • Purification and characterization of acetylcholinesterase from desert cobra (Walterinnesia aegyptia) venom.

    abstract::Acetylcholinesterase (AChE) has been identified and purified from the venom of desert cobra (W aegyptia) to apparent homogeneity using a TSK G 3000 SW gel filtration column and a Mono Q anion-exchange column. AChE was purified to homogeneity as established by sodium dodecylsulfate/polyacrylamide gel electrophoresis. T...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(96)81328-9

    authors: Duhaiman AS,Alhomida AS,Rabbani N,Kamal MA,al-Jafari AA

    更新日期:1996-01-01 00:00:00

  • Regulation of translation and cell growth by eIF-4E.

    abstract::This review discusses the regulation of a key controlling step in the initiation of protein synthesis, the binding of mRNA to ribosomes. Particular focus is given to the phosphorylation of the cap-binding factor, eIF-4E, and the role of this factor in the regulation of cell growth. ...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/0300-9084(94)90185-6

    authors: Sonenberg N

    更新日期:1994-01-01 00:00:00

  • The crystal structure of ferritin from Chlorobium tepidum reveals a new conformation of the 4-fold channel for this protein family.

    abstract::Ferritins are ubiquitous iron-storage proteins found in all kingdoms of life. They share a common architecture made of 24 subunits of five α-helices. The recombinant Chlorobium tepidum ferritin (rCtFtn) is a structurally interesting protein since sequence alignments with other ferritins show that this protein has a si...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2014.07.019

    authors: Arenas-Salinas M,Townsend PD,Brito C,Marquez V,Marabolli V,Gonzalez-Nilo F,Matias C,Watt RK,López-Castro JD,Domínguez-Vera J,Pohl E,Yévenes A

    更新日期:2014-11-01 00:00:00

  • GTP-dependent modification of a 21-kDa substrate with NAD+ in bovine brain soluble fraction is not ADP-ribosylation of small G-protein but tailing of tRNA.

    abstract::Labeling of 21-kDa material was observed when bovine brain soluble fraction was incubated with [adenylate-32P]NAD+ in the presence of GTP. The 21-kDa substrate, slightly smaller than C3 substrate in size, was labeled even without C3 exoenzyme. GTP could be replaced by nucleoside triphosphates other than ATP while ATP ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(97)86150-0

    authors: Badruzzaman M,Miyaoka T,Terashima M,Yamada K,Ohno T,Yamamori C,Tsuchiya M,Shimoyama M

    更新日期:1997-07-01 00:00:00

  • Synthesis of a brain-specific protein (S100 protein) in a lectin-resistant mutant of a rat glial cell line (C6).

    abstract::The synthesis of S100 protein increases toward the end of the exponential phase of growth of clonal rat glial cells C6 in monolayer culture. Moreover the synthesis of this protein can be increased by treatment of C6 cells with the lectin succinylated concanavalin A (succinyl ConA). In order to study the relationship b...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(84)80024-3

    authors: Marks A,Law J,Mahony J

    更新日期:1983-11-01 00:00:00

  • New nomenclature of the non-rod-shaped lactic acid bacteria.

    abstract::Bacteria of the Lactococcus (formerly lactic Streptococcus) genus are described to emphasize certain less well-known facts of importance in their successful use as dairy starters. Other streptococci (non-rod forms) are briefly characterized to emphasize their usefulness in industrial milk fermentations. ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(88)90087-9

    authors: Sandine WE

    更新日期:1988-04-01 00:00:00

  • Molecular-level insights into aging processes of skin elastin.

    abstract::Skin aging is characterized by different features including wrinkling, atrophy of the dermis and loss of elasticity associated with damage to the extracellular matrix protein elastin. The aim of this study was to investigate the aging process of skin elastin at the molecular level by evaluating the influence of intrin...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2016.08.010

    authors: Mora Huertas AC,Schmelzer CE,Hoehenwarter W,Heyroth F,Heinz A

    更新日期:2016-09-01 00:00:00