Abstract：
:Bacterial survive and respond to adverse changes in the environment by regulating gene transcription through two-component regulatory systems. In Salmonella Typhimurium the STM1485 gene expression is induced under low pH (4.5) during replication inside the epithelial host cell, but it is not involved in sensing or resisting to this condition. Since the RcsCDB system is activated under acidic conditions, we investigated whether this system is able to modulate STM1485 expression. We demonstrated that acid-induced activation of the RcsB represses STM1485 transcription by directly binding to the promoter. Under the same condition, the RstA regulator activates the expression of this gene. Physiologically, we observed that RcsB-dependent repression is required for the survival of bacteria when they are exposed to pancreatic fluids. We hypothesized that STM1485 plays an important role in Salmonella adaptation to pH changes, during transition in the gastrointestinal tract. We suggest that bacteria surviving the gastrointestinal environment invade the epithelial cells, where they can remain in vacuoles. In this new environment, acidity and magnesium starvation activate the expression of the RstA regulator in a PhoPQ-dependent manner, which in turn induces STM1485 expression. These levels of STM1485 allow increased bacterial replication within vacuoles to continue the course of infection.
journal_name
Biochimiejournal_title
Biochimieauthors
Torrez Lamberti MF,Farizano JV,López FE,Martínez Zamora MG,Pescaretti MM,Delgado MAdoi
10.1016/j.biochi.2019.02.005subject
Has Abstractpub_date
2019-05-01 00:00:00pages
46-54eissn
0300-9084issn
1638-6183pii
S0300-9084(19)30044-6journal_volume
160pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:The automation of the mica surface displacement of a surface force apparatus (SFA) increases the accuracy of the intermolecular force measurements that is obtained without the presence of the experimenter. The automatic device cancels any thermal drift and the stability of the mica surfaces is optimum. The distance be...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(00)80007-3
更新日期：1998-05-01 00:00:00
abstract：:Despite its biological importance, iron is a pro-oxidant element and its accumulation results in tissue injury. Iron overload diseases such as thalassemia and hereditary hemochromatosis are commonly associated with liver tissue injury. Glutamyl cysteine (GC) is a dipeptide with antioxidant properties owing to its cyst...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2015.06.006
更新日期：2015-08-01 00:00:00
abstract：:Embryonic stem cells (ESCs) are capable of unlimited self-renewal and differentiation into multiple cell types. Recent large-scale analyses have identified various cell surface molecules on ESCs. Some of them are considered to be beneficial markers for characterization of cellular phenotypes and/or play an essential r...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.10.017
更新日期：2012-01-01 00:00:00
abstract：:We have purified, by a three step procedure, a yeast C-nitrosoreductase and studied its action on p-nitroso-N,N-dimethylaniline. Microcalorimetric measurements performed on the microcalorimeter LKB "batch", have been given the number of protons exchanged during the reaction : four protons are involved. Two are given b...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(75)80132-5
更新日期：1975-01-01 00:00:00
abstract：:Glycosylphosphatidylinositol phospholipase D (GPI-PLD) has been proposed to be responsible for cleaving membrane-associated glycosylphosphatidyl inositol (GPI) molecules to generate inositol phosphoglycan (IPGs), which have growth factor-mimetic properties. We have cloned the mouse liver GPI-PLD cDNA, which has a sequ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2004.04.003
更新日期：2004-04-01 00:00:00
abstract：:A high level of subcellular compartmentalization is a hallmark of eukaryotic cells. This intricate internal organization was present already in the common ancestor of all extant eukaryotes, and the determination of the origins and early evolution of the different organelles remains largely elusive. Organellar proteome...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.03.021
更新日期：2015-12-01 00:00:00
abstract：:The molecular basis for the essential role of cholesterol in mammalian (and other cholesterol-requiring) cells has long been the object of intense interest. Cholesterol has been found to modulate the function of membrane proteins critical to cellular function. Current literature supports two mechanisms for this modula...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(91)90093-g
更新日期：1991-10-01 00:00:00
abstract：:We have recently reported that α(2)β(1) and α(1)β(1) isozymes of Na(+)/K(+)-ATPase (NKA) are localized in the caveolae whereas only the α(1)β(1) isozyme of NKA is localized in the non-caveolae fraction of pulmonary artery smooth muscle cell membrane. It is well known that different isoforms of NKA are regulated differ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.12.020
更新日期：2012-04-01 00:00:00
abstract：:The primary kinetic isotope effect of the reaction catalyzed by NAD+-dependent formate dehydrogenase (EC 1.2.1.2.) from the methylotrophic bacterium Pseudomonas sp. 101 has been studied. Analysis of the ratios HVm/DVm and H(Vm/KM)/D(Vm/KM) in the pH range 6.1-7.9 showed that the transfer of hydride ion in ternary enzy...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(89)90186-7
更新日期：1989-04-01 00:00:00
abstract：:The lysosomal aspartic protease cathepsin D (cath-D) is overexpressed and hyper-secreted by epithelial breast cancer cells. This protease is an independent marker of poor prognosis in breast cancer as it is correlated with the incidence of clinical metastasis. In normal cells, cath-D is localized in intracellular vesi...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2010.05.009
更新日期：2010-11-01 00:00:00
abstract：:The recF gene of E coli lies within a cluster of genes which play essential roles in DNA replication; the gene order is dnaA dnaN recF gyrB. Each of these genes has its own promoters which, with the exception of dnaA promoters, reside entirely within the translated region of the respective preceding gene. In this repo...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(91)90220-u
更新日期：1991-02-01 00:00:00
abstract：:The potent cytotoxicity of reactive oxygen species (ROS) can cause various diseases, however, it may also serve as a powerful chemotherapeutic strategy capable of killing cancer cells. Oxalomalate (OMA, α-hydroxy-β-oxalosuccinic acid), a tricarboxylic acid intermediate, is a well-known competitive inhibitor of two cla...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.01.004
更新日期：2019-03-01 00:00:00
abstract：:Single cell oils (SCOs) are now produced by various microorganisms as commercial sources of arachidonic acid (ARA) and docosahexaenoic acid (DHA). These oils are now used extensively as dietary supplements in infant formulas. An understanding of the underlying biochemistry and genetics of oil accumulation in such micr...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2004.09.017
更新日期：2004-11-01 00:00:00
abstract：:We have analyzed changes in the supercoiling of nucleoid DNA of murine fibroblasts cultured in the presence of hydralazine. The entire DNA attached to the nuclear matrix was extracted from the cells and sedimented in neutral sucrose density gradients containing ethidium bromide. Nucleoids from cells treated with hydra...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(98)80025-4
更新日期：1998-07-01 00:00:00
abstract：:The purpose of the present study was to investigate the effect of a concentrated preparation (EPA 30) containing eicosapentaenoic acid (EPA, 20:5 n-3) and docosahexaenoic acid (DHA, 22:6 n-3) on the limiting desaturation steps of the polyunsaturated fatty acid biosynthesis in spontaneously hypertensive rats (SHR). Adu...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(97)81505-2
更新日期：1997-02-01 00:00:00
abstract：:Labeling of 21-kDa material was observed when bovine brain soluble fraction was incubated with [adenylate-32P]NAD+ in the presence of GTP. The 21-kDa substrate, slightly smaller than C3 substrate in size, was labeled even without C3 exoenzyme. GTP could be replaced by nucleoside triphosphates other than ATP while ATP ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(97)86150-0
更新日期：1997-07-01 00:00:00
abstract：:Phospholipid analysis of the membranes associated with fast sedimenting folded chromosomes prepared by lysis of E. coli CR 34 shows that both inner and outer membranes are parts of the complex, in proportions not very different from that found in the whole bacteria. During the preparation of the folded chromosomes, th...
journal_title：Biochimie
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:Ceramidase plays an important role in regulating the metabolism of sphingolipids, such as ceramide, sphingosine (SPH), and sphingosine-1-phosphate (S1P), by controlling the hydrolysis of ceramide. Here we report the cloning and biochemical characterization of a neutral ceramidase from the red flour beetle Tribolium ca...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.03.009
更新日期：2011-07-01 00:00:00
abstract：:The polysaccharide mixture obtained by hot water extraction of Aloe vahombe leaves is composed of at least four different paritally acetylated glucomannans which differ in molecular weight, glucose to mannose ratios and acetyl contents. Furthermore, one fraction contains a small but significant amount of protein which...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(86)80187-0
更新日期：1986-09-01 00:00:00
abstract：:The effect of adenosine was tested on the energetic metabolism of fed rat liver cells after isolation. The cells were incubated in a buffered saline medium with glucose (5 mM) and adenosine (1 mM) for 30 minutes at 37 degrees C. This increased the concentration of the adenylic nucleotides ATP (+57 per cent, ADP (+39 p...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(80)80286-0
更新日期：1979-01-01 00:00:00
abstract：:Protein 3D structures support their biological functions. As the number of protein structures is negligible in regards to the number of available protein sequences, prediction methodologies relying only on protein sequences are essential tools. In this field, protein secondary structure prediction (PSSPs) is a mature ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.09.006
更新日期：2020-12-01 00:00:00
abstract：:The study of modified nucleoside contributions to RNA chemistry, structure and function has been thwarted by the lack of a site-selected method of incorporation which is both versatile and adaptable to present synthetic technologies. A reproducible and versatile site-selected incorporation of nine differently modified...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(96)88115-6
更新日期：1995-01-01 00:00:00
abstract：:Lipid membranes have an essential role in most physiological processes including cell protection, cell-to-cell communication and regulation of intracellular signaling. These multiple roles of biological membranes prompted the study and the development of artificial lipid membranes with the primary aim to reconstitute ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2018.11.011
更新日期：2019-02-01 00:00:00
abstract：:Influenza virus-associated RNA polymerase is composed of one molecule each of three viral P proteins and carries the complete activity of capped RNA-primed vRNA-directed transcription. The RNA polymerase holoenzyme also carries capped RNA endonuclease to generate capped oligonucleotide primers for transcription and 3'...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(97)86735-1
更新日期：1996-01-01 00:00:00
abstract：:A synthetic phosphonate inhibitor designed for lipase inhibition but displaying a broader range of activity was covalently immobilized on a solid support to generate a function-directed tool targeting serine hydrolases. To achieve this goal, straightforward and reliable analytical techniques were developed, allowing t...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.07.015
更新日期：2014-12-01 00:00:00
abstract：:An 18.2 kDa protein from the liver fluke, Fasciola hepatica has been identified and characterised. The protein shows strongest sequence similarity to egg antigen proteins from Schistosoma mansoni, Schistosoma japonicum and Clonorchis sinensis. The protein is predicted to adopt a calmodulin-like fold; it thus represent...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.06.015
更新日期：2012-11-01 00:00:00
abstract：:Over the last couple of decades, the PPAR family of transcription factors has received much attention from the pharmaceutical industry due to their profound ability to improve glucose and lipid metabolism upon agonist activation. However, more recently the interest in these nuclear receptors has faded because several ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2016.10.017
更新日期：2017-05-01 00:00:00
abstract：:In order to study the relationship between bile cholesterol and free cholesterol carried by high and low density lipoproteins (HDL and LDL), 10 male Wistar rats, 11 weeks old and fed with a standard diet were divided into 3 groups which received an intravenous infusion (jugular vein) of either LDL, HDL or liposomes. L...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(87)90270-7
更新日期：1987-01-01 00:00:00
abstract：:The major pterin in Tetrahymena pyriformis, strain W, earlier suggested to be L-threo-biopterin and named ciliapterin [1] is now identified as D-threo-neopterin (D-monapterin). This is the first example of a natural D-monapterin. This compound was characterized by its chromatographic behavior, its fluorescence propert...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(91)90089-j
更新日期：1991-10-01 00:00:00
abstract：:Thyroid peroxidase is a member of a family of mammalian peroxidases that includes myeloperoxidase, lactoperoxidase, eosinophil peroxidase, and salivary peroxidase. Protein sequences showing a high degree of sequence similarity with mammalian peroxidases have recently been observed in several invertebrate species. A mu...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(99)80110-2
更新日期：1999-05-01 00:00:00
