Abstract:
:The primary kinetic isotope effect of the reaction catalyzed by NAD+-dependent formate dehydrogenase (EC 1.2.1.2.) from the methylotrophic bacterium Pseudomonas sp. 101 has been studied. Analysis of the ratios HVm/DVm and H(Vm/KM)/D(Vm/KM) in the pH range 6.1-7.9 showed that the transfer of hydride ion in ternary enzyme-substrate complex is a limiting step of the reaction, and the formate binding to the binary complex (formate dehydrogenase + NAD+) reached equilibrium when the pH of the medium was increased. An approach has been developed to determine the elementary constants of substrate association (kon) and dissociation (koff) at the stages of the binary--ternary enzyme-substrate complexes for the random equilibrium 2-substrate kinetic mechanism. The kon and koff values obtained for the bacterial formate dehydrogenase by using the proposed approach for NAD+ were (4.8 +/- 0.8)*10(5)M-1s-1 and (90 +/- 10) s-1, and for formate (2.0 +/- 1.0)*10(4) M-1s-1 and (60 +/- 20) s-1, respectively.
journal_name
Biochimiejournal_title
Biochimieauthors
Tishkov VI,Galkin AG,Egorov AMdoi
10.1016/0300-9084(89)90186-7subject
Has Abstractpub_date
1989-04-01 00:00:00pages
551-7issue
4eissn
0300-9084issn
1638-6183pii
0300-9084(89)90186-7journal_volume
71pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::A nuclear poly(ADP-ribose) polymerase (PARP) is activated by gamma-irradiation and consequently synthesizes poly(ADP-ribose) by binding to DNA strand-breaks. This property suggests that PARP is a DNA strand-break-signal generator. Meanwhile, the cell-cycle arrest occurs in G1 and G2 phases following gamma-irradiation....
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(96)88161-2
更新日期:1995-01-01 00:00:00
abstract::A series of halomethylated derivatives of dihydrocoumarins has been found to inhibit irreversibly proteases and esterases. alpha-Chymotrypsin, subtilish, elastase are rapidly inactivated in the presence of these compounds, while trypsin, kallicrein, papain are inhibited more slowly. Esterases like acetylcholinesterase...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(77)80140-5
更新日期:1977-01-01 00:00:00
abstract::Since 2012, the interest for TMEM165 increased due to its implication in a rare genetic human disease named TMEM165-CDG (Congenital Disorder(s) of Glycosylation). TMEM165 is a Golgi localized protein, highly conserved through evolution and belonging to the uncharacterized protein family 0016 (UPF0016). Although the pr...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.07.016
更新日期:2019-10-01 00:00:00
abstract::A number of specific Fab and Fv fragments and their complexes with antigens (avian lysozymes), haptens, and anti-idiotopic Fabs have been studied by immunochemical and crystallographic techniques. Antigen and antibody interact through closely complementary contacting surfaces, without major conformational changes. An ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90114-v
更新日期:1990-08-01 00:00:00
abstract::After exhaustive pronase digestion, purification by gel filtration and affinity chromatography on concanavalin A, three glycopeptide fractions were obtained from rat hemopexin. Two fractions (I and II) were concanavalin A non-reactive and one (III) was concanavalin A reactive. On the basis of carbohydrate composition,...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(83)80083-2
更新日期:1983-03-01 00:00:00
abstract::RNA editing alters genomically encoded cytidines to uridines posttranscriptionally in higher plant mitochondria. Most of these editing events occur in translated regions and consequently alter the amino acid sequence. In Oenothera berteriana more than 500 editing sites have been detected and the total number of editin...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(96)88108-9
更新日期:1995-01-01 00:00:00
abstract::Adrenoleukodystrophy (ALD) is an X-linked peroxisomal disorder characterized by a progressive demyelination of the central nervous system and adrenal insufficiency. Clinical phenotypes of different severity are frequently observed within the same kindred. ALD is characterized biochemically by the accumulation of very-...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(93)90089-b
更新日期:1993-01-01 00:00:00
abstract::Human mitochondrial aminoacyl-tRNA synthetases (mt-aaRSs), the enzymes which esterify tRNAs with the cognate specific amino acid, form mainly a different set of proteins than those involved in the cytosolic translation machinery. Many of the mt-aaRSs are of bacterial-type in regard of sequence and modular structural o...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.02.005
更新日期:2009-05-01 00:00:00
abstract::Hypertension is the leading cause of preventable mortality worldwide, contributing to over 9 million deaths per annum, predominantly owing to cardiovascular disease. The association of obesity, physical inactivity and alcohol with elevated blood pressure (BP) is firmly established. Weight loss or other dietary strateg...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2020.03.016
更新日期:2020-06-01 00:00:00
abstract::A neoglycolipid of structure beta-D-Glcp-S-(CH2)3N(OH)(CH2)4-O-cholest-5-en-3 beta-yl has been prepared in fair overall yield by reduction of the nitrone obtained by condensation of beta-D-Glcp-S-(CH2)3NHOH and OCH-(CH2)3-O-cholest-5-en-3 beta-yl. This synthetic procedure is very flexible, allowing a large range of le...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(92)90184-g
更新日期:1992-01-01 00:00:00
abstract::MicroRNAs (miRNAs) are ∼21 nt non-coding small RNAs which regulate gene expression at the post-transcriptional level in plants and animals. Until recently, only limited numbers of miRNAs were identified in Cucurbitaceae, a large flowering plant family. In this study, 220 potential miRNA candidates were identified from...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.03.002
更新日期:2014-07-01 00:00:00
abstract::An efficient phosphotriester methodology based on the use of condensing agents in the presence of several O-nucleophilic catalysts has been developed. ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80169-3
更新日期:1985-07-01 00:00:00
abstract::Sodium channel alpha subunit type 1 (SCN1A) is voltage gated ion channel which plays critical role in membrane excitability. A common SCN1A IVS5-91G>A (rs3812718) allele has been attributed to be a possible modifying factor for epilepsy susceptibility and therapeutic response. In the present study, we enrolled 485 epi...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.02.006
更新日期:2013-06-01 00:00:00
abstract::Stroke is a worldwide major cause of mortality and morbidity. Preclinical studies have identified over 1000 molecules with brain-protective properties. More than 200 clinical trials have evaluated neuroprotective candidates for ischemic stroke yet, to date almost all failed, leading to a re-analysis of treatment strat...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2015.06.005
更新日期:2016-01-01 00:00:00
abstract::Injection of 3,5,3' L-triiodothyronine (15 micrograms/100 g) induces a biphasic enhancement of rat heart ornithine decarboxylase (EC. 4.1.17) activity after 4 and 21 hours. This induction is observed after each daily injection, but to a lesser extent. The properties of partially purified basal enzyme and induced enzym...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(82)80383-0
更新日期:1982-11-01 00:00:00
abstract::Epigenetic alterations are involved in every step of carcinogenesis. The development of chromatin-modifying agents (CMAs) has provided the ability to fight cancer by reversing these alterations. Currently, four CMAs have been approved for cancer treatment; two DNA demethylating agents and two deacetylase inhibitors. A...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2012.05.012
更新日期:2012-11-01 00:00:00
abstract::The emergence of drug-resistant Mycobacterium tuberculosis (Mtb) stains has escalated the need for developing more efficient drugs and therapeutic strategies against tuberculosis. Here we functionally annotate a secretory mycobacterial asparaginase Rv1538c (MtA) and describe its biochemical properties. MtA primarily e...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.12.023
更新日期:2021-01-05 00:00:00
abstract::Identification of proteoglycans in two human malignant mesothelioma cell lines, one with epithelial differentiation and the other with fibroblast-like phenotype, and the effects of epidermal (EGF), insulin-like (IGF-I) and platelet-derived (PDGF-BB) growth factors on the synthesis of hyaluronan (HA) and proteoglycans ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(99)80131-x
更新日期:1999-07-01 00:00:00
abstract::Flavodoxins are one domain alpha/beta electron transfer proteins that participate in photosynthetic reactions. All flavodoxins carry a molecule of flavin mononucleotide (FMN), non-covalently bound, that confers redox properties to the protein. There are two structurally distinct flavodoxins, short ones and long flavod...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(00)88876-8
更新日期:1998-10-01 00:00:00
abstract::The chromatin of shrimp hepatopancreas has been extracted from isolated nuclei and characterized. Nuclei were prepared in the presence of Cu++ and phenyl methyl sulfonyl fluoride in order to inhibit the nuclease and protease activities throughout the different purification steps. The purified nuclei are heterogenous i...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(79)80133-9
更新日期:1979-01-01 00:00:00
abstract::As part of a project aimed at obtaining selective inhibitors and drug-like compounds targeting tRNA synthetases from trypanosomatids, we have elucidated the crystal structure of human cytosolic histidyl-tRNA synthetase (Hs-cHisRS) in complex with histidine in order to be able to compare human and parasite enzymes. The...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.08.005
更新日期:2014-11-01 00:00:00
abstract::The mechanism of action on rabbit red cells of Treponema hyodysenteriae hemolysin was studied using volume analysis and release of hemoglobin. While fixation of the hemolysin on the erythrocytes is temperature independent, it appears that hemolysis is temperature dependent. The kinetics of hemolysis proceed according ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(80)80134-9
更新日期:1980-01-01 00:00:00
abstract::The amino acid sequence of rat thymus histone obtained in highly purified form by preparative electrophoresis, was determined. This sequence is identical to the sequence of calf thymus histone H2B. The in vitro phosphorylation of the rat histone with a cyclic AMP-dependent protein kinase isolated from rat pancreas led...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(79)80313-2
更新日期:1979-01-01 00:00:00
abstract::Several inositol isomers and in particular myo-inositol (MI) and D-chiro-inositol (DCI), were shown to possess insulin-mimetic properties and to be efficient in lowering post-prandial blood glucose. In addition, abnormalities in inositol metabolism are associated with insulin resistance and with long term microvascula...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2013.05.011
更新日期:2013-10-01 00:00:00
abstract::Two soluble forms of brain glutamate dehydrogenase isoproteins were inactivated by pyridoxal 5'-phosphate. Restoration of catalytic activity can be accomplished by dialysis and addition of an excess of cysteine or lysine. Spectral evidence is presented to indicate that the inactivation proceeds through Schiff base for...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)84333-7
更新日期:1996-01-01 00:00:00
abstract::Glycosphingolipids and sphingolipids of cellular plasma membranes (PMs) reach luminal intra-lysosomal vesicles (LVs) for degradation mainly by pathways of endocytosis. After a sorting and maturation process (e.g. degradation of sphingomyelin (SM) and secretion of cholesterol), sphingolipids of the LVs are digested by ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2016.05.004
更新日期:2016-11-01 00:00:00
abstract::Amyloid beta protein (beta/A4) is deposited in senile plaques of patients with Alzheimer's disease. This protein is derived from a larger membrane-associated protein, termed amyloid precursor protein (APP). The constitutive processing of APP occurs at the central portion of beta/A4, resulting in the release of large N...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(93)90048-w
更新日期:1993-01-01 00:00:00
abstract::The interactions of 6-8 ring hairpin polyamides (PAs) with the minor groove of DNA have been investigated extensively. More recent studies of large antiviral PAs (14-20 rings) active against small DNA tumor viruses lead to questions regarding the extent to which the DNA binding behaviors of the well studied, smaller P...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.07.021
更新日期:2019-10-01 00:00:00
abstract::Microalgae can produce large quantities of triacylglycerols (TAGs) and other neutral lipids that are suitable for making biofuels and as feedstocks for green chemistry. However, TAGs accumulate under stress conditions that also stop growth, leading to a trade-off between biomass production and TAG yield. Recently, in ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2019.06.016
更新日期:2020-02-01 00:00:00
abstract::Histone acetyltransferase (Hpa2) is an unusual acetyltransferase, with a wide range of substrates; including histones, polyamines and aminoglycosides antibiotic. Hpa2 belongs to GNAT superfamily and GNATs are well known for the formation of homo-oligomers. However, the reason behind their oligomerization remained unex...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.11.015
更新日期:2020-01-01 00:00:00