Abstract：
:Injection of 3,5,3' L-triiodothyronine (15 micrograms/100 g) induces a biphasic enhancement of rat heart ornithine decarboxylase (EC. 4.1.17) activity after 4 and 21 hours. This induction is observed after each daily injection, but to a lesser extent. The properties of partially purified basal enzyme and induced enzyme, at 21h, after single injections have been compared. 1) Affinity for ornithine is the same for both enzymes, but affinity for pyridoxal-phosphate is 40-fold higher for the induced one. 2) Thermostability studies suggest that basal and induced enzymes have different conformations. 3) The two enzymes have similar immuno-reactivity. 4) The comparisons of the time-dependent activity curve after injection and of the antigen/activity ratio suggests that triiodothyronine induces the synthesis of new molecules of enzymes and that an inhibition of the enzyme activity also occurs which explains the biphasic induction.
journal_name
Biochimiejournal_title
Biochimieauthors
Raymondjean M,Kneip B,Bachner L,Bogdanovsky-Sequeval D,Schapira Gdoi
10.1016/s0300-9084(82)80383-0subject
Has Abstractpub_date
1982-11-01 00:00:00pages
1027-34issue
11-12eissn
0300-9084issn
1638-6183pii
S0300-9084(82)80383-0journal_volume
64pub_type
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