In vitro conversion of porcine thyroid cells growing in monolayer into functional follicular cells.

abstract：:Glucose-6 phosphatase (G6Pase), a key enzyme of glucose homeostasis, catalyses the hydrolysis of glucose-6 phosphate (G6P) to glucose and inorganic phosphate. A deficiency in G6Pase activity causes type 1 glycogen storage disease (GSD-1), mainly characterised by hypoglycaemia. Genetic analyses of the two forms of this...

journal_title：Biochimie

authors： Soty M,Chilloux J,Casteras S,Grichine A,Mithieux G,Gautier-Stein A

更新日期：2012-03-01 00:00:00

abstract：:For efficient DNA hydrolysis, Type III restriction endonuclease EcoP15I interacts with two inversely oriented recognition sites in an ATP-dependent process. EcoP15I consists of two methylation (Mod) subunits and a single restriction (Res) subunit yielding a multifunctional enzyme complex able to methylate or to hydrol...

journal_title：Biochimie

authors： Mackeldanz P,Alves J,Möncke-Buchner E,Wyszomirski KH,Krüger DH,Reuter M

更新日期：2013-04-01 00:00:00

abstract：:The interactions between lactic acid bacteria and their phages are commercially significant. Current research has focused on the elucidation of the mechanisms and genetics of phage resistance. Phage resistance genes have been linked to plasmid DNA for Streptococcus lactis and Streptococcus cremoris, and preliminary st...

journal_title：Biochimie

authors： Sanders ME

更新日期：1988-03-01 00:00:00

abstract：:Labeling of 21-kDa material was observed when bovine brain soluble fraction was incubated with [adenylate-32P]NAD+ in the presence of GTP. The 21-kDa substrate, slightly smaller than C3 substrate in size, was labeled even without C3 exoenzyme. GTP could be replaced by nucleoside triphosphates other than ATP while ATP ...

journal_title：Biochimie

authors： Badruzzaman M,Miyaoka T,Terashima M,Yamada K,Ohno T,Yamamori C,Tsuchiya M,Shimoyama M

更新日期：1997-07-01 00:00:00

abstract：:The ascomycete Cladosporium herbarum is a prominent fungal inducer of Type I allergy. The only major allergen identified so far is Cla h 8, a NADP-dependent mannitol dehydrogenase (MtDH). MtDH, a cytoplasmic protein of 28.5kDa, belongs to the Short chain Dehydrogenases/Reductases (SDR), acting as a NADP-dependent oxid...

journal_title：Biochimie

authors： Nüss D,Goettig P,Magler I,Denk U,Breitenbach M,Schneider PB,Brandstetter H,Simon-Nobbe B

更新日期：2010-08-01 00:00:00

abstract：:Fluorometry using a substrate DNA labeled with a single fluorophore (6-carboxyfluorescein) at the 3'-end of the processed strand was shown to be a useful tool for monitoring DNA-binding and 3'-processing activities of HIV-1 and PFV integrases (INs). The DNA binding to either of the INs resulted in a fluorescence signa...

journal_title：Biochimie

authors： Anisenko A,Agapkina J,Zatsepin T,Yanvarev D,Gottikh M

更新日期：2012-11-01 00:00:00

abstract：:The phosphotriesterase PTE, identified in the soil bacterium Pseudomonas diminuta, is thought to have evolved in the last several decades to degrade the pesticide paraoxon with proficiency approaching the limit of substrate diffusion (k(cat)/K(M) of 4 x 10(7)M(-1)s(-1)). It belongs to the amidohydrolase superfamily, b...

journal_title：Biochimie

authors： Porzio E,Merone L,Mandrich L,Rossi M,Manco G

更新日期：2007-05-01 00:00:00

abstract：:The emergence of drug-resistant Mycobacterium tuberculosis (Mtb) stains has escalated the need for developing more efficient drugs and therapeutic strategies against tuberculosis. Here we functionally annotate a secretory mycobacterial asparaginase Rv1538c (MtA) and describe its biochemical properties. MtA primarily e...

journal_title：Biochimie

authors： Kataria A,Patel AK,Kundu B

更新日期：2021-01-05 00:00:00

abstract：:The termination of protein synthesis in bacteria requires codon-specific polypeptide release factors RF-1 (UAG/UAA specific) and RF-2 (UGA/UAA specific). We have proposed that release factors mimic tRNA and recognize the stop codon for polypeptide release (Nakamura et al (1996) Cell 87, 147-150). In contrast to the te...

journal_title：Biochimie

authors： Uno M,Ito K,Nakamura Y

更新日期：1996-01-01 00:00:00

abstract：:Diadenosine 5',5'''-p1,p4-tetraphosphate (Ap4A) alpha, beta-phosphorylase from yeast Saccharomyces cerevisiae catalyzes two reactions: Ap4A cleavage and nucleoside diphosphate--phosphate (NDP-Pi) exchange. In both reactions phosphate can be substituted by arsenate, chromate, molybdate, tungstate or vanadate. In the pr...

journal_title：Biochimie

authors： Guranowski A,Blanquet S

更新日期：1986-05-01 00:00:00

abstract：:About 150 participants attended the symposium organised at the Palais de la Bourse in Bordeaux, France on September 22-23, 2017. Thirty speakers from all over the world delivered lectures covering selection processes, aptamer chemistry and innovative applications of these powerful tools that display major advantages o...

journal_title：Biochimie

authors： Toulmé JJ,Azéma L,Darfeuille F,Dausse E,Durand G,Paurelle O

更新日期：2018-02-01 00:00:00

abstract：:L-aromatic aminoacid decarboxylase has been purified more than thousand times from homogenates of rat brain, in several steps : centrifugation, DEAE-cellulose, CM cellulose, hydroxylapatite, DEAE sephadex. Its properties have been studied, most of them on an intermediate fraction of the purification, because of the in...

journal_title：Biochimie

authors： Corgier M,Pacheco H

更新日期：1975-01-01 00:00:00

abstract：:The targets of in vivo studies of the ribosomal assembly process are mainly the events of rRNA processing, whereas in vitro studies (total reconstitution) focus on principles of the assembly process such as assembly-initiation proteins, rate-limiting steps and a detailed sequence of assembly reactions (assembly map). ...

journal_title：Biochimie

authors： Nierhaus KH

更新日期：1991-06-01 00:00:00

abstract：:A d-carbamoylase from Sinorhizobium morelens S-5 was purified and characterized. The enzyme was purified 189-fold to homogeneity with a yield of 19.1% by aqueous two-phase extraction and two steps of column chromatography. The enzyme is a homotetramer with a native molecular mass of 150 kDa and a subunit relative mole...

journal_title：Biochimie

authors： Wu S,Liu Y,Zhao G,Wang J,Sun W

更新日期：2006-03-01 00:00:00

abstract：:Isolation and properties of B. subtilis ts 19 mutant, isolated as thermosensitive for sporulation, are described. At the non permissive temperature (42degreesC), the mutant cells are blocked at stage zero of sporulation and do not excrete extracellular enzymes such as serylprotease and esterase. At the permissive temp...

journal_title：Biochimie

authors： Millet J,Larribe M,Aubert JP

更新日期：1976-01-01 00:00:00

abstract：:Directed mutagenesis has been used to study the nicotinamide subsite of the glycolytic NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Residue Asn313 is involved together with the carboxyamide moiety of the nicotinamide ring in a complex network of hydrogen bonding interactions which fix the position o...

journal_title：Biochimie

authors： Corbier C,Mougin A,Mely Y,Adolph HW,Zeppezauer M,Gerard D,Wonacott A,Branlant G

更新日期：1990-08-01 00:00:00

abstract：:The mouse interferon alpha 7 gene, the signal sequence of which has been removed by oligonucleotide-directed mutagenesis, was introduced into a Bacillus subtilis secretion vector containing the promoter and the signal sequence of the B. subtilis levansucrase gene. Different B. subtilis strains were transformed with th...

journal_title：Biochimie

authors： Dion M,Rapoport G,Doly J

更新日期：1989-06-01 00:00:00

abstract：:Posttranscriptional gene regulation by small RNAs and its crucial impact on development, apoptosis, stem cell self-renewal and differentiation gained tremendous scientific attention since the discovery of RNA interference (RNAi) and microRNAs (miRNAs). However, in the last few years, many more examples for regulatory ...

journal_title：Biochimie

authors： Röther S,Meister G

更新日期：2011-11-01 00:00:00

abstract：:pH-Dependence of hydrolytic activity of trypsin has been studied in cationic reverse micellar system of cetyltrimethylammonium bromide (CTAB) in (50% v/v) chloroform/isooctane using a positively charged substrate N(alpha)-benzoyl-L-arginine ethyl ester (BAEE). The pH of the medium was varied from 4.0 to 8.5 with addit...

journal_title：Biochimie

authors： Fadnavis NW,Babu RL,Deshpande A

更新日期：1998-12-01 00:00:00

abstract：:The open reading frame PA3859 of Pseudomonas aeruginosa encodes an intracellular carboxylesterase belonging to a group of microbial enzymes (EC 3.1.1.1) that catalyze the hydrolysis of aliphatic and aromatic esters with a broad substrate specificity. With few exceptions, for this class of enzymes, belonging to the α/β...

journal_title：Biochimie

authors： Pesaresi A,Lamba D

更新日期：2010-12-01 00:00:00

abstract：:Long non-coding RNAs constitute the most abundant part of the transcribed mammalian genome. lncRNAs affect all essential processes in the living cell including transcription, splicing, translation, replication, shaping of chromatin and post translational modification of proteins. Alterations in lncRNA expression have ...

journal_title：Biochimie

authors： Smekalova EM,Kotelevtsev YV,Leboeuf D,Shcherbinina EY,Fefilova AS,Zatsepin TS,Koteliansky V

更新日期：2016-12-01 00:00:00

abstract：:Theoretic and experimental arguments are surveyed which justify the setting up, within the family of pyridoxal-P-dependent lyases, of a special subgroup that comprizes several enzymes catalyzing exclusively beta-replacement reactions of alpha-aminoacids with electronegative substituents in the beta-position. The autho...

journal_title：Biochimie

authors： Braunstein AE,Goryachenkova EV

更新日期：1976-01-01 00:00:00

abstract：:The oxidation of beta-D-glucose with glucose oxidase generally requires oxygen, which, under normal conditions is present at low concentrations in the reaction medium. Experiments show that glucose oxidase is no longer saturated by oxygen at enzyme concentrations greater than 0.4 mg.ml1. This is due to the decrease in...

journal_title：Biochimie

authors： Boudrant J,Cheftel C

更新日期：1975-01-01 00:00:00

abstract：:Beta-turmerin from turmeric (Curcuma longa) waste grits obtained after extraction of curcumin was purified by successive gel permeation chromatography. Homogeneity of beta-turmerin was confirmed by its movement as single band both in SDS-PAGE and as well as in native (basic) PAGE. The apparent molecular mass is approx...

journal_title：Biochimie

authors： Smitha S,Dhananjaya BL,Dinesha R,Srinivas L

更新日期：2009-09-01 00:00:00

abstract：:Lysosomal cysteine proteinases were fractionated from partially purified rat muscle lysosomes. By gel filtration on Sephadex G75, cathepsin D was separated from two thiol-requiring proteolytic fractions of Mr 25 000 and 55 000, respectively. By chromatofocusing, the first fraction (Mr = 25 000) was resolved into three...

journal_title：Biochimie

authors： Obled A,Ouali A,Valin C

更新日期：1984-09-01 00:00:00

abstract：:An immunological demonstration of alpha-fetoprotein (AFP) in uterine cytosol from immature rats is reported. The identity between serum AFP and the estrogen binding 4.5 S macromolecular complex of uterine cytosol was demonstrated by the use of a specific immunoadsorbent to AFP. Analysis of the sedimentation profile ...

journal_title：Biochimie

authors： Aussel C,Uriel J,Michel G,Baulieu EE

更新日期：1974-01-01 00:00:00

abstract：:The effect of alpha-tocopherol on the structure and thermotropic phase behaviour of 1-palmitoyl-2-oleoyl-phosphatidylethanolamine dispersed in excess water was examined by synchrotron X-ray diffraction and differential scanning calorimetry. Small- and wide-angle X-ray scattering intensity profiles were recorded from m...

journal_title：Biochimie

authors： Wang X,Quinn PJ

更新日期：2006-12-01 00:00:00

abstract：:The term 'RNA editing' was used for the first time in 1986 to describe the process of uridylate insertion into trypanosomal mitochondrial transcripts. Since then, the term has been used more generally to describe a large variety of processes involving base insertions, deletions and conversions that generate RNAs with ...

journal_title：Biochimie

authors： Blanc V,Jordana X,Litvak S,Araya A

更新日期：1996-01-01 00:00:00

abstract：:Triple-negative breast cancer (TNBC) is a heterogeneous group of breast cancer with one common feature: distinctly metastatic nature with higher rate of relapse and shorter survival compared with other subtypes of breast cancer. The epithelial to mesenchymal transition (EMT) is highly associated with cancer metastasis...

journal_title：Biochimie

authors： Liang L,Liu X,He J,Shao Y,Liu J,Wang Z,Xia L,Han T,Wu P

更新日期：2019-07-01 00:00:00

abstract：:The short isoform of Rho guanine nucleotide exchange factor ARHGEF5 is known as TIM, which plays diverse roles in, for example, tumorigenesis, neuronal development and Src-induced podosome formation through the activation of its substrates, the Rho family of GTPases. The activation is auto-inhibited by a putative heli...

journal_title：Biochimie

authors： He P,Tan DL,Liu HX,Lv FL,Wu W

更新日期：2015-04-01 00:00:00