Abstract:
:The termination of protein synthesis in bacteria requires codon-specific polypeptide release factors RF-1 (UAG/UAA specific) and RF-2 (UGA/UAA specific). We have proposed that release factors mimic tRNA and recognize the stop codon for polypeptide release (Nakamura et al (1996) Cell 87, 147-150). In contrast to the textbook view, genetic experiments have indicated that Escherichia coli RF-2 terminates translation very weakly at UAA while Salmonella RF-2 decodes this signal efficiently. Moreover, an excess of E coli RF-2 was toxic to cells while an excess of Salmonella RF-2 was not. These two RF-2 proteins are identical except for 16 out of 365 amino acids. Fragment swap experiments and site-directed mutagenesis revealed that a residue at position 246 is solely responsible for these two phenotypes. Upon substituting Ala (equivalent to Salmonella RF-2) for Thr-246 of E coli RF-2, the protein acquired increased release activity for UAA as well as for UGA. These results led us to conclude that E coli RF-2 activity is potentially weak and that the amino acid at position 246 plays a crucial role, not for codon discrimination, but for stop codon recognition or polypeptide release, presumably constituting an essential moiety of tRNA mimicry or interacting with peptidyltransferase centers of the ribosome.
journal_name
Biochimiejournal_title
Biochimieauthors
Uno M,Ito K,Nakamura Ydoi
10.1016/s0300-9084(97)86715-6subject
Has Abstractpub_date
1996-01-01 00:00:00pages
935-43issue
11-12eissn
0300-9084issn
1638-6183pii
S0300-9084(97)86715-6journal_volume
78pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Mitochondrial function requires genes encoded in both mitochondrial and nuclear genomes. Tfam, the activator of mammalian mitochondrial transcription, is encoded in the nucleus and its expression has been shown in in vitro studies to be controlled by nuclear respiratory factors NRF-1 and NRF-2. In order to understand ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(99)00223-0
更新日期:1999-10-01 00:00:00
abstract::High glucose (HG)-induced podocyte injury contributes to the pathogenesis of diabetic nephropathy, a severe complication of diabetes. Bromodomain-containing protein 4 (BRD4) has emerged as a critical regulator for cell injury. However, whether BRD4 participates in HG-induced podocyte injury remains unclear. In this st...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.07.012
更新日期:2019-10-01 00:00:00
abstract::Unspliced human immunodeficiency virus-1 (HIV-1) mRNA is capped and therefore can be translated via conventional scanning mechanism. In addition, its 5' untranslated region (5'UTR) is thought to function as an internal ribosome entry site (IRES) during G2/M-phase of cell cycle or when cap-dependent translation is inhi...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.12.004
更新日期:2016-02-01 00:00:00
abstract::In this work, were studied the conditions of erucic acid (cis-docosenoic, n-9) shortening by using Rat liver mitochondrial preparations which were incubated in vitro with [14-14C] erucic acid (22:1), with inhibitors of the respiratory chain (rotenone, cyanide) or not, with activators of either the shortening reaction ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(82)80352-0
更新日期:1982-10-01 00:00:00
abstract::Esterases and lipases both hydrolyse ester bonds. Whereas the lipases display high activity towards the aggregated state of its substrate, the esterases typically show highest activity towards the soluble state of its substrate. We have compared the amino acid sequence, the 3D-structure as well as the pH-dependent ele...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(00)01188-3
更新日期:2000-11-01 00:00:00
abstract::Halophilic proteins have evolved to be soluble, stable and active in high salt concentration. Crystallographic studies have shown that surface enrichment by acidic amino acids is a common structural feature of halophilic proteins. In addition, ion-binding sites have also been observed in most of the cases. The role of...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2007.03.008
更新日期:2007-08-01 00:00:00
abstract::Nanoluciferase (NanoLuc) is a newly developed small luciferase reporter with the brightest bioluminescence reported to date. In the present work, we developed NanoLuc as a novel quantitative protein fusion tag for efficient overexpression in Escherichia coli and ultrasensitive bioluminescent assays using human leukemi...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.08.012
更新日期:2014-11-01 00:00:00
abstract::We extracted from L. biflexa patoc a fraction F, reacting in hemagglutination and ring tests with sera prepared against more than ten different serogroups. This fraction contains mainly a polysaccharide (65 per cent), the role of which was clearly demonstrated in the precipitation reaction with homologous antisera, th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80313-6
更新日期:1976-01-01 00:00:00
abstract::Using a new extended set of 13 amber suppressors in E coli, systematic amino-acid replacements were performed at positions 104(E) and 238(G) of TEM-1 beta-lactamase from PUC19. The enzyme is tolerant to most substitutions tested at position 104. Missense revertants E104K, E104S or E104Y exhibited only minor changes in...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90073-p
更新日期:1990-06-01 00:00:00
abstract::The lignan 8,8'-bis-(methylenedioxy)cinnamic acid (BMDCA) is a powerful competitive inhibitor (K1 = 2.0 microM) of the lignin peroxidase (LiP) from Phanerochaete chrysosporium and of the extracellular peroxidase of Phlebia radiata (I0.5 = 10 microM). BMDCA derivatives with the same double bond system also inhibited th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(96)88186-7
更新日期:1995-01-01 00:00:00
abstract::Bikunin (Bk) is a Künitz-type serine proteinase inhibitor, which occurs in human plasma, mainly as covalent complexes with one or two of the three peptide heavy chains. The leading member of this glycoprotein family is inter-alpha-inhibitor (I alpha I), which consists of two heavy chains (H1 and H2) linked to Bk. Bk c...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(03)00066-x
更新日期:2003-01-01 00:00:00
abstract::Administration of beta-adrenergic agonists to domestic species can lead to skeletal muscle hypertrophy, probably by reducing the rate of myofibrillar protein breakdown. Myofibrillar breakdown is associated with the calcium-dependent proteinase system (calpains I,II and calpastatin) whose activity also changes during b...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(92)90125-x
更新日期:1992-03-01 00:00:00
abstract::Ferritins are ubiquitous iron-storage proteins found in all kingdoms of life. They share a common architecture made of 24 subunits of five α-helices. The recombinant Chlorobium tepidum ferritin (rCtFtn) is a structurally interesting protein since sequence alignments with other ferritins show that this protein has a si...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.07.019
更新日期:2014-11-01 00:00:00
abstract::Three trends are seen in the organization of ribosomal DNA genes during evolution: 1) gradual separation and separability of the regulation of transcription of 5S and larger subunit rRNAs; 2) retention of a transcription unit containing both large and small rRNAs; and 3) clustering of genes for both 5S and 18S-28S rDN...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(91)90042-y
更新日期:1991-06-01 00:00:00
abstract::Latices from Asclepias spp are used in wound healing and the treatment of some digestive disorders. These pharmacological actions have been attributed to the presence of cysteine proteases in these milky latices. Asclepias curassavica (Asclepiadaceae), "scarlet milkweed" is a perennial subshrub native to South America...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.07.017
更新日期:2009-11-01 00:00:00
abstract::A synthetic gene of yeast aspartic acid tRNA with a promoter for phage T7 RNA polymerase was cloned in Escherichia coli. The in vitro transcribed tRNA(Asp) molecules are deprived of modified nucleotides and retain their aspartylation capacity. The solution conformation of these molecules was mapped with chemical struc...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90158-d
更新日期:1990-10-01 00:00:00
abstract::Clustered apurinic/apyrimidinic (AP) sites are more cytotoxic than isolated AP lesions because double strand breaks (DSB) can be formed during repair of closely positioned bistranded AP sites. Formation of DSB due to simultaneous cleavage of bistranded AP sites may be regulated by proteins specifically interacting wit...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.04.027
更新日期:2018-07-01 00:00:00
abstract::The efficiency of oxidative phosphorylation was estimated in intact resting cells of Escherichia coli K 12, strain PA 601 (chl-s) and its chl-r mutants, all of them grown anaerobically in the presence of nitrate. The oxidation of endogenous NADH in intact chl-s cells was accompanied by the formation of ATP whatever th...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(77)80316-7
更新日期:1977-01-01 00:00:00
abstract::A human replication initiation protein, Cdt1, is a central player in the cell cycle regulation of DNA replication, and geminin down-regulates Cdt1 function by direct binding. It has been demonstrated that Cdt1 hyperfunction resulting from Cdt1-geminin imbalance, for example, by geminin silencing with small interfering...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2008.02.018
更新日期:2008-06-01 00:00:00
abstract::Nucleolin (NCL) is one of the most abundant non ribosomal protein of the nucleolus where it plays a central role in polymerase I transcription. NCL is also found outside of the nucleolus, in the nucleoplasm, cytoplasm as well as on the cell membrane. It acts in all cell compartments to control cellular homeostasis and...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2015.03.023
更新日期:2015-06-01 00:00:00
abstract::The Ty1 retrotransposon of the yeast Saccharomyces cerevisiae is a long terminal repeat mobile genetic element that transposes through an RNA intermediate. Initiation of minus-strand and plus-strand DNA synthesis are two critical steps during reverse transcription of the retrotransposon genome. Initiation of minus-str...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(96)80013-7
更新日期:1996-01-01 00:00:00
abstract::The NADPH-oxidase complex, which plays beneficial or detrimental role in the inflammatory and degenerative diseases, is a membrane multi-subunit complex tightly regulated in order to produce superoxide anions, precursor of oxygen reactive species (ROS), in cells. The flavocytochrome b(558) (Cytb(558)) is the catalytic...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.10.006
更新日期:2014-12-01 00:00:00
abstract::To elucidate the metabolic function of mRNA polyadenylation in Escherichia coli. we searched for a polyadenylate-binding protein as a potential mediator of the function of the poly(A) moiety. Using a nitrocellulose filter-binding assay and a Northwestern blot technique, a protein in the ribosomal supernatant fraction ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)82741-1
更新日期:1997-09-01 00:00:00
abstract::After 30 years of effort, the mammalian adenylate cyclase catalytic subunit has now been purified. It is a glycoprotein of 155 kDa, representing less than 0.01% of synaptosomal membrane protein. As measured in the presence of forskolin, its specific activity is 10-20 mumol of cAMP X mg-1 X min-1. The enzyme obtained i...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(87)90016-2
更新日期:1987-04-01 00:00:00
abstract::Isolation and properties of B. subtilis ts 19 mutant, isolated as thermosensitive for sporulation, are described. At the non permissive temperature (42degreesC), the mutant cells are blocked at stage zero of sporulation and do not excrete extracellular enzymes such as serylprotease and esterase. At the permissive temp...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80361-6
更新日期:1976-01-01 00:00:00
abstract::Experimental evidence accumulated to date by several research groups indicates that antisense oligodeoxynucleotides targeted against messenger RNA (mRNA) sequences located downstream of the initiation codon fail to inhibit the translation of this mRNA unless the hybrid is cleaved by RNase H. It has previously been sho...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(93)90032-n
更新日期:1993-01-01 00:00:00
abstract::In man the brain represents about 2% of the body weight, but contains 25% of the body's cholesterol. Cholesterol itself does not cross the blood-brain barrier and is synthesised in situ. Excess cholesterol from brain is exported in the form of oxysterols, or metabolised to steroids, which in contrast to cholesterol ca...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2006.10.008
更新日期:2007-02-01 00:00:00
abstract::The inhibitory action on rat liver phosphoglycerate kinase of structural analogs of the two substrates of this enzyme (beta,gamma- methyleneadenosine 5'-triphosphate for ATP and 2-phosphoglycerate for 3-phosphoglycerate) was studied. In the direction of ADP utilization, the inhibition patterns obtained with beta,gamma...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(86)80201-2
更新日期:1986-04-01 00:00:00
abstract::Oxidative stress, characterized by the accumulation of reactive oxygen species (ROS), is known to have numerous detrimental effects on the myocardium such as the induction of apoptotic cell death, hypertrophy, fibrosis, dysfunction, and dilatation. Over the past several years, we have shown that mitochondrial NADP(+)-...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.11.016
更新日期:2014-04-01 00:00:00
abstract::Mammalian aminoacylase-1 (Acy1) participates in the breakdown of N-acetylated amino acids during intracellular protein catabolism. Acy1 is most abundantly expressed in the kidney tubular epithelium. Lately, Acy1 deficiency was identified in children with increased urinary excretion of several N-acetylamino acids. Here...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2007.12.006
更新日期:2008-05-01 00:00:00