Abstract:
:The term 'RNA editing' was used for the first time in 1986 to describe the process of uridylate insertion into trypanosomal mitochondrial transcripts. Since then, the term has been used more generally to describe a large variety of processes involving base insertions, deletions and conversions that generate RNAs with a primary sequence different to those encoded by the gene. RNA editing has been observed in the mitochondrial fraction of trypanosomes, plants and other organisms, in the animal nuclear fraction in the case of the apolipoprotein B and glutamate brain receptors mRNAs as well as in viruses like paramyxovirus, hepatitis delta and probably HIV. The role of cytidine and adenine deamination leading to C to U and A to I transitions has became pivotal to explain this process by base conversion. In this review we will focus mainly on the work performed in our group on plant mitochondria and more specifically on the mechanism and the functional significance of RNA editing in wheat organelles. The original contributions of our laboratory in this field are: i) showing that RNA editing is reflected at the protein level; ii) settling three in vitro systems to assay C to U conversion using a wheat mitochondrial lysate as source of enzymes and factors, and unedited mRNA from the same source, as substrate; iii) determination by double labelling of the unedited substrate that RNA editing in wheat mitochondria occurs via a deamination step; and iv) that introducing unedited proteins in the mitochondria of transgenic plants leads to the emergence of cytoplasmic male sterility supporting the idea that the role of this process is to produce functional proteins. Using the antisense approach in transgenic plants we were able to obtain a significant male fertility restoration.
journal_name
Biochimiejournal_title
Biochimieauthors
Blanc V,Jordana X,Litvak S,Araya Adoi
10.1016/0300-9084(96)84757-2subject
Has Abstractpub_date
1996-01-01 00:00:00pages
511-7issue
6eissn
0300-9084issn
1638-6183pii
0300-9084(96)84757-2journal_volume
78pub_type
杂志文章,评审相关文献
BIOCHIMIE文献大全abstract::The misfolding of amyloid proteins is closely correlated with the pathogenesis of protein conformation-related diseases, such as Alzheimer's disease (AD), prion disease, and type 2 diabetes mellitus (T2DM). The deposition of human islet amyloid polypeptide (hIAPP) and amyloid-β (Aβ) protein is entangled in AD and diab...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.04.012
更新日期:2020-07-01 00:00:00
abstract::The lysosomal aspartic protease cathepsin D (cath-D) is overexpressed and hyper-secreted by epithelial breast cancer cells. This protease is an independent marker of poor prognosis in breast cancer as it is correlated with the incidence of clinical metastasis. In normal cells, cath-D is localized in intracellular vesi...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2010.05.009
更新日期:2010-11-01 00:00:00
abstract::We identified a T-to-C mutation 2 nucleotides (nt) upstream from the AG in a GT-AG intron between exons 2 and 3 in the human T-cell leukemia virus type I (HTLV-I) tax mRNA. This mutation resulted in the preferential usage of an alternative splice site, causing a 75-nt elongation of tax mRNA and reduced production of v...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.07.013
更新日期:2006-02-01 00:00:00
abstract::The issue of whether histone H1 possesses specificity of binding to certain nucleotide sequences in DNA is of fundamental importance to the suggested role of the linker histone in the regulation of gene transcription. The purpose of the present study was to reinvestigate the specificity of binding of histone H1 to the...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(93)90116-a
更新日期:1993-01-01 00:00:00
abstract::A human replication initiation protein, Cdt1, is a central player in the cell cycle regulation of DNA replication, and geminin down-regulates Cdt1 function by direct binding. It has been demonstrated that Cdt1 hyperfunction resulting from Cdt1-geminin imbalance, for example, by geminin silencing with small interfering...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2008.02.018
更新日期:2008-06-01 00:00:00
abstract::Acetylcholinesterase (AChE) has been identified and purified from the venom of desert cobra (W aegyptia) to apparent homogeneity using a TSK G 3000 SW gel filtration column and a Mono Q anion-exchange column. AChE was purified to homogeneity as established by sodium dodecylsulfate/polyacrylamide gel electrophoresis. T...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(96)81328-9
更新日期:1996-01-01 00:00:00
abstract::Apolipoprotein E (apoE) has been genetically linked to late-onset Alzheimer's disease. From the three common alleles (epsilon2, epsilon3 and epsilon4), epsilon4 has been suggested to promote amyloid beta (Ass) plaque fibrillation, one hallmark of Alzheimer's disease. It has been demonstrated that altered lipid content...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.10.007
更新日期:2006-05-01 00:00:00
abstract::Sequence analysis of the Lutzomyia (Lu.) ayacuchensis salivary gland cDNA library identified a short peptide containing an RGD (Arg-Gly-Asp) sequence flanked by two cysteine residues in the C-terminal end as the most abundant transcript. In the present study, a recombinant protein of the RGD-containing peptide, design...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.02.011
更新日期:2015-05-01 00:00:00
abstract::In order to study the role of the cytosolic form of phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) (PEPCK-C) in skeletal muscle, PEPCK-Cmus mice were created by introducing the cDNA for the enzyme, linked to the human alpha-skeletal actin gene promoter, into their germ line. Two founder lines generated by this ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2008.03.009
更新日期:2008-06-01 00:00:00
abstract::A reliable phylogenetic reconstruction of the evolutionary history of contemporary species depends on a robust identification of the universal common ancestor (UCA) at the root of the Tree of Life (ToL). That root polarizes the tree so that the evolutionary succession of ancestors to descendants is discernable. In eff...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2017.04.014
更新日期:2017-07-01 00:00:00
abstract::Oxidative stress, characterized by the accumulation of reactive oxygen species (ROS), is known to have numerous detrimental effects on the myocardium such as the induction of apoptotic cell death, hypertrophy, fibrosis, dysfunction, and dilatation. Over the past several years, we have shown that mitochondrial NADP(+)-...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.11.016
更新日期:2014-04-01 00:00:00
abstract::During the suckling period, the rats are fed continuously with milk, which is a high-fat low-carbohydrate diet (HF). At weaning, milk is progressively replaced by the rat's laboratory chow which is a high-carbohydrate low-fat diet (HCHO), and this is accompanied by large hormonal modifications: an increase in plasma i...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(91)90077-e
更新日期:1991-01-01 00:00:00
abstract::Purified chromatin from pig lymph nodes has been prepared and used for the isolation of chromatin proteins and non-histine proteins. Saccharose nuclei from this tissue have been used for the preparation of nuclear phenol-soluble phosphoproteins. The isolated proteins have been compared by analytical electrophoresis in...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80391-4
更新日期:1976-01-01 00:00:00
abstract::A synthetic gene of yeast aspartic acid tRNA with a promoter for phage T7 RNA polymerase was cloned in Escherichia coli. The in vitro transcribed tRNA(Asp) molecules are deprived of modified nucleotides and retain their aspartylation capacity. The solution conformation of these molecules was mapped with chemical struc...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(90)90158-d
更新日期:1990-10-01 00:00:00
abstract::A Binding Protein (BP) of nearly 95,000 daltons interacts with the acid phosphatase at the permissive temperature, elevating its molecular weight from 48,000 +/- 2,000 to 136,000 +/- 7,000 in CL and CHR strains of Physarum polycephalum plasmodia. The same molecule has not been observed in the extracts of M3C VIII and ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(83)80160-6
更新日期:1983-06-01 00:00:00
abstract::The binding properties of Mg2+, Mn2+ and Co2+ to yeast mitochondrial oligomycin sensitive ATPase complex are studied, as reflected by their catalytic effect (hydrolysis of ATP or pNPP, a pseudo substrate) or by a physical parameter (atomic absorption, electron paramagnetic reasonance of Mn2+, enhanced fluorescence of ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(78)80701-9
更新日期:1977-01-01 00:00:00
abstract::The Escherichia coli low molecular mass penicillin-binding proteins (PBP4, PBP5 and PBP6) are a group of penicillin-sensitive enzymes involved in the final stages of cell wall assembly. It has been suggested that these proteins may interact with the periplasmic face of the inner membrane via C-terminal amphiphilic alp...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)83502-x
更新日期:1997-04-01 00:00:00
abstract::A major incentive in inhibitor research is that control of limited proteolysis constitutes a valuable pharmacological tool. Protease inhibitors have proved to be successful in influencing pathogenesis in many experimental models but a breakthrough to use in human therapy has mainly been restricted to aprotinin and ang...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(91)90084-e
更新日期:1991-01-01 00:00:00
abstract::The preadipocyte cell lines 3T3-L1 and 3T3-F442A are widely used to study the cellular mechanisms of preadipocyte differentiation and mature adipocyte functions. However, transfection with naked DNA is inefficient in these cell lines. Adenoviral gene transfer is a powerful technique to induce high levels of transgene ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.05.010
更新日期:2005-11-01 00:00:00
abstract::The redox homeostasis of cytoplasm is maintained by a series of disulfide exchange reactions mediated by proteins belonging to the thioredoxin superfamily. Thioredoxin and thioredoxin reductase, being the major members of the family, play a key role in oxidative stress response of Staphylococcus aureus. In this report...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.01.019
更新日期:2019-05-01 00:00:00
abstract::Pterocarpans, a family of isoflavonoids found in the diverse Fabaceae, display potent cytotoxic activity over a panel of tumor cell lines, and among those tested, 2,3,9- trimethoxypterocarpan displays the most potent activity. This study evaluates the effects of 2,3,9-trimethoxypterocarpan and its related derivatives ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.06.005
更新日期:2014-09-01 00:00:00
abstract::Betaine aldehyde dehydrogenase from the human opportunistic pathogen Pseudomonas aeruginosa (PaBADH) catalyzes the irreversible, NAD(P)(+)-dependent oxidation of betaine aldehyde, producing glycine betaine, an osmoprotectant. PaBADH participates in the catabolism of choline and likely in the defense against the osmoti...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.09.022
更新日期:2011-02-01 00:00:00
abstract::Latices from Asclepias spp are used in wound healing and the treatment of some digestive disorders. These pharmacological actions have been attributed to the presence of cysteine proteases in these milky latices. Asclepias curassavica (Asclepiadaceae), "scarlet milkweed" is a perennial subshrub native to South America...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.07.017
更新日期:2009-11-01 00:00:00
abstract::Synthetic antiestrogens such as Tamoxifen (Nolvadex) are currently used to treat postmenopausal breast cancer. Their mechanism of action is reviewed at the cellular and molecular level, through data of the literature and the current view of the author. Synthetic antiestrogens are mostly acting directly on breast cance...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(82)80411-2
更新日期:1982-02-01 00:00:00
abstract::We have purified, by a three step procedure, a yeast C-nitrosoreductase and studied its action on p-nitroso-N,N-dimethylaniline. Microcalorimetric measurements performed on the microcalorimeter LKB "batch", have been given the number of protons exchanged during the reaction : four protons are involved. Two are given b...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(75)80132-5
更新日期:1975-01-01 00:00:00
abstract::Dihydroorotate dehydrogenase (DHODH) is the fourth enzyme in the de novo pyrimidine biosynthetic pathway and has been exploited as the target for therapy against proliferative and parasitic diseases. In this study, we report the crystal structures of DHODH from Leishmania major, the species of Leishmania associated wi...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.04.003
更新日期:2012-08-01 00:00:00
abstract::The gene for the ribosomal protein L5 from the archaebacterium Sulfolobus acidocaldarius has been isolated and sequenced. The gene codes for a basic protein of molecular weight 29 165 Da. This protein shows substantial similarity to the equivalent protein from other archaebacteria as well as from yeast, and considerab...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90047-5
更新日期:1991-06-01 00:00:00
abstract::The nucleotide sequence of the gene encoding chicken ribosomal protein L7a was determined. The gene contains eight exons and seven introns, which spread over 3613 nucleotides. The transcription initiation sites were located on four consecutive nucleotides GCCC, which are at the 5' terminus of a short polypyrimidine tr...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(93)90128-f
更新日期:1993-01-01 00:00:00
abstract::Specific contacts between DNA phosphate groups and positively charged nucleophilic amino acids from the Escherichia coli Fpg protein play a significant role in DNA-Fpg protein interaction. In order to identify these phosphate groups the chemical crosslinking procedure was carried out. The probing of the Fpg protein ac...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(03)00067-1
更新日期:2003-05-01 00:00:00
abstract::In man the brain represents about 2% of the body weight, but contains 25% of the body's cholesterol. Cholesterol itself does not cross the blood-brain barrier and is synthesised in situ. Excess cholesterol from brain is exported in the form of oxysterols, or metabolised to steroids, which in contrast to cholesterol ca...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2006.10.008
更新日期:2007-02-01 00:00:00