Characterization of papain-like isoenzymes from latex of Asclepias curassavica by molecular biology validated by proteomic approach.

abstract：:Apoptosis is the physiological process by which unwanted cells in an organism are killed. Bcl-2, a membrane-bound cytoplasmic protein, and its close relative Bcl-xL, are both effective inhibitors of apoptosis induced by a wide variety of stimuli in many different cell types. In a previous study, we reported that suppr...

journal_title：Biochimie

authors： Cherbonnel-Lasserre C,Dosanjh MK

更新日期：1997-10-01 00:00:00

abstract：:Glycosphingolipids and sphingolipids of cellular plasma membranes (PMs) reach luminal intra-lysosomal vesicles (LVs) for degradation mainly by pathways of endocytosis. After a sorting and maturation process (e.g. degradation of sphingomyelin (SM) and secretion of cholesterol), sphingolipids of the LVs are digested by ...

journal_title：Biochimie

authors： Sandhoff K

更新日期：2016-11-01 00:00:00

abstract：:Over the last decades, mesenchymal stem cells (MSCs) have been extensively studied with regard to their potential applications in regenerative medicine. In rheumatic diseases, MSC-based therapy is the subject of great expectations for patients who are refractory to proposed treatments such as rheumatoid arthritis (RA)...

journal_title：Biochimie

authors： Maumus M,Jorgensen C,Noël D

更新日期：2013-12-01 00:00:00

abstract：:Rhodospirillum salexigens is a moderately halophilic purple phototrophic bacterium which grows optimally in 8% NaCl. The amino acid sequences of the two principal soluble cytochromes c have been determined. One of these is a cytochrome c2, similar in size to mitochondrial cytochrome c. While clearly of the same sequen...

journal_title：Biochimie

authors： Ambler RP,Daniel M,Meyer TE,Kamen MD

更新日期：1994-01-01 00:00:00

abstract：:The repressible Saccharomyces cerevisiae acid phosphatase (APase) coded by the PHO5 gene is a cell wall protein that follows the yeast secretory pathway. We had previously described the in vivo fate of a multicopy plasmid-encoded modified protein, lacking 15 out of 17 signal peptide amino acids. This modified protein ...

journal_title：Biochimie

authors： Silve S,Volland C,Hinnen A,Haguenauer-Tsapis R

更新日期：1990-02-01 00:00:00

abstract：:The Gag-encoded nucleocapsid protein NCp7 (72 amino acids) from HIV-1, the regulatory protein, Vpr (96 amino acids) and numerous derivatives have been synthesized by solid phase method and their structures determined by 2D NMR. In NCp7, the two highly folded zinc fingers of the Cx2Cx4Hx4C type are in close spacial pro...

journal_title：Biochimie

authors： Roques BP,Morellet N,de Rocquigny H,Déméné H,Schueler W,Jullian N

更新日期：1997-11-01 00:00:00

abstract：:Phosphorus metabolites and intracellular pH have been examined in the slime mold Dictyostelium discoideum by non-destructive 31P-NMR measurements. In a spectrum from a suspension of aerobic amoebae, the major peaks are inorganic phosphate, nucleotide di- and triphosphates. In the corresponding perchloric acid extract,...

journal_title：Biochimie

authors： Satre M,Klein G,Martin JB

更新日期：1986-12-01 00:00:00

abstract：:The major pterin in Tetrahymena pyriformis, strain W, earlier suggested to be L-threo-biopterin and named ciliapterin [1] is now identified as D-threo-neopterin (D-monapterin). This is the first example of a natural D-monapterin. This compound was characterized by its chromatographic behavior, its fluorescence propert...

journal_title：Biochimie

authors： Klein R,Tatischeff I,Tham G,Grolière CA

更新日期：1991-10-01 00:00:00

abstract：:Triiodothyronine (T3), injected daily into rats, induces heart hypertrophy. In a recent work we have shown that a single T3 injection stimulates RNA synthesis and modifies the translational products of myocardial mRNAs in reticulocyte lysates, quantitatively and qualitatively. In this work we show that T3 induces smal...

journal_title：Biochimie

authors： Raymondjean M,Leibovitch SA,Leibovitch MP,Harel J,Kruh J

更新日期：1984-09-01 00:00:00

abstract：:Enterocin CRL35 activity is deeply influenced by the membrane viscosity as could be demonstrated performing determinations of the minimal inhibitory concentrations (MIC) at different temperatures and analyzing the membrane viscosity in these cells as well as in resistant bacteria. In all the cases, bacteriocin activit...

journal_title：Biochimie

authors： Minahk CJ,Saavedra L,Sesma F,Morero R

更新日期：2005-02-01 00:00:00

abstract：:The metabolism of the phospholipids of the submaxillary gland are studied after intraperitoneal injections of [32P]-orthophosphate in several groups of rats. The gland contains mainly phosphatidylcholines and phosphatidylethanolamines and in smaller quantities phosphatidylinositols, phosphatidylserines, sphingomyelins...

journal_title：Biochimie

authors： Leclercq C,Di Costanzo G

更新日期：1976-01-01 00:00:00

abstract：:Purified chromatin isolated from lymphocytic cells derived from patients with acute leukemia, or other lymphoproliferative disorders has been compared with chromatin isolated from normal human lymphocytic cells by gel electrophoresis and differential gradient ultracentrifugation. Thermal denaturation studies showed hi...

journal_title：Biochimie

authors： Desai LS,Wulff UC,Foley GE

更新日期：1975-01-01 00:00:00

abstract：:Human α(1)-acid glycoprotein (AGP) is a positive acute phase plasma protein containing two disulfide bridges. Structural studies have shown that under specific conditions AGP undergoes aggregation. In this study, we analysed the nature of AGP's aggregates formed under reducing and non-reducing conditions at pH 5.5 and...

journal_title：Biochimie

authors： Scirè A,Baldassarre M,Galeazzi R,Tanfani F

更新日期：2013-02-01 00:00:00

abstract：:Catalase (E.C 1.11.1.6) was purified from leaves of Zandedeschia aethiopica to apparent homogeneity by a one-step hydrophobic interaction chromatography on a phenyl Sepharose CL-4B column. The purified enzyme preparation was obtained with a final recovery of enzyme activity of about 61% and a specific activity of 146 ...

journal_title：Biochimie

authors： Trindade H,Karmali A,Pais MS

更新日期：1988-12-01 00:00:00

abstract：:Recombinant plasmids containing head-to-tail copies of different coat-protein replacement genomes of wheat dwarf virus (WDV) were used to study the mechanism leading to the release of replicating unit-length molecules in suspension culture cells of Triticum monococcum. For plasmids bearing two complete genomes, the vi...

journal_title：Biochimie

authors： Heyraud F,Matzeit V,Schaefer S,Schell J,Gronenborn B

更新日期：1993-01-01 00:00:00

abstract：:Ten new defensins have been isolated from seeds of Triticum kiharae and related species of the Triticum and Aegilops genera by a combination of chromatographic procedures including affinity-, size-exclusion, and reversed-phase high-performance liquid chromatography. Nine were completely sequenced and shown to represen...

journal_title：Biochimie

authors： Odintsova TI,Egorov TA,Musolyamov AKh,Odintsova MS,Pukhalsky VA,Grishin EV

更新日期：2007-05-01 00:00:00

abstract：:Antimicrobials targeting the fatty acid synthesis (FASII) pathway are being developed as alternative treatments for bacterial infections. Emergence of resistance to FASII inhibitors was mainly considered as a consequence of mutations in the FASII target genes. However, an alternative and efficient anti-FASII resistanc...

journal_title：Biochimie

authors： Morvan C,Halpern D,Kénanian G,Pathania A,Anba-Mondoloni J,Lamberet G,Gruss A,Gloux K

更新日期：2017-10-01 00:00:00

abstract：:Using a rapid FPLC procedure for the separation of calpain I, calpain II and the inhibitor calpastatin from pork meat, results of proteolytic and inhibitory activity were obtained. Duplicate measurements showed variation coefficients less than 0.13. Compared to others we found lower calpain I activity 1 h post-mortem....

journal_title：Biochimie

authors： Iversen P,Ertbjerg P,Larsen LM,Monllao S,Møller AJ

更新日期：1993-01-01 00:00:00

abstract：:The proteasome activators known as 11S REG or PA28 were discovered about 10 years ago. They are homo- or heteroheptameric rings that bind to the ends of 20S proteasomes and activate cleavage of peptides but not folded proteins. In this article, we focus on structural features of three homologous REG subunits (termed a...

journal_title：Biochimie

authors： Li J,Rechsteiner M

更新日期：2001-03-01 00:00:00

abstract：:High-mobility group box-1 (HMGB1) is remarkably mobile in living cells, which reflects its ability to interact only transiently with both DNA and protein. This property is likely essential for HMGB1 nuclear activities. Nonetheless the weak interaction of HMGB1 with DNA and/or protein partners has also been a major lim...

journal_title：Biochimie

authors： Messmer M,Klein C,Boniface R,Gnädig NF,Lecerf M,Barnay-Verdier S,Maréchal V

更新日期：2013-04-01 00:00:00

abstract：:Porphyromonas gingivalis uses a type IX secretion system (T9SS) to deliver more than 30 proteins to the bacterial surface using a conserved C-terminal domain (CTD) as an outer membrane translocation signal. On the surface, the CTD is cleaved and an anionic lipopolysaccharide (A-PLS) is attached by PorU sortase. Among ...

journal_title：Biochimie

authors： Veillard F,Sztukowska M,Nowakowska Z,Mizgalska D,Thøgersen IB,Enghild JJ,Bogyo M,Potempa B,Nguyen KA,Potempa J

更新日期：2019-11-01 00:00:00

abstract：:Determination of the physicochemical parameters of unilamellar and multilamellar liposomes from concentration data in light of a recent quantitative biodynamic concept which strictly adheres to the CGS unit system (cm, g, s) and the mass/volume chemical unit, mol.cm-3. ...

journal_title：Biochimie

authors： Hauton JC,Martigne M,Domingo N,Lafont H

更新日期：1987-05-01 00:00:00

abstract：:Mitochondrial membranes are essential for the good functioning of the organelle. For instance, the inner mitochondrial membrane contains the oxidative phosphorylation system that permits ATP synthesis. Phospholipids environment and especially cardiolipin are crucial for the mitochondrial energy metabolism. Indeed, car...

journal_title：Biochimie

authors： Dumas JF,Peyta L,Couet C,Servais S

更新日期：2013-01-01 00:00:00

abstract：:The term 'RNA editing' was used for the first time in 1986 to describe the process of uridylate insertion into trypanosomal mitochondrial transcripts. Since then, the term has been used more generally to describe a large variety of processes involving base insertions, deletions and conversions that generate RNAs with ...

journal_title：Biochimie

authors： Blanc V,Jordana X,Litvak S,Araya A

更新日期：1996-01-01 00:00:00

abstract：:SR-PSOX can function as a scavenger receptor, a chemokine and an adhesion molecule, and it could be an interesting player in the formation of atherosclerotic lesions. Our previous studies demonstrated that basic amino acid residues in the chemokine domain of SR-PSOX are critical for its functions. In this study the co...

journal_title：Biochimie

authors： Liu W,Yin L,Dai Y

更新日期：2013-02-01 00:00:00

abstract：:Organisms exposed to ionizing radiation (IR) undergo increases in the production of reactive oxygen species (ROS), which are determinant components in the induction of apoptosis. Sensitive to apoptosis gene (SAG) encodes a redox-inducible and apoptosis-protective antioxidant protein. This report demonstrates that the ...

journal_title：Biochimie

authors： Kim SY,Yang ES,Lee YS,Lee J,Park JW

更新日期：2011-02-01 00:00:00

abstract：:Unspliced human immunodeficiency virus-1 (HIV-1) mRNA is capped and therefore can be translated via conventional scanning mechanism. In addition, its 5' untranslated region (5'UTR) is thought to function as an internal ribosome entry site (IRES) during G2/M-phase of cell cycle or when cap-dependent translation is inhi...

journal_title：Biochimie

authors： Smirnova VV,Terenin IM,Khutornenko AA,Andreev DE,Dmitriev SE,Shatsky IN

更新日期：2016-02-01 00:00:00

abstract：:Seryl-tRNA synthetases (SerRS) covalently attach serine to cognate tRNA(Ser). Atypical SerRSs, considerably different from canonical enzymes, have been found in methanogenic archaea. A crystal structure of methanogenic-type SerRS revealed a motif within the active site (serine ordering loop; SOL), which undergoes a no...

journal_title：Biochimie

authors： Dulic M,Pozar J,Bilokapic S,Weygand-Durasevic I,Gruic-Sovulj I

更新日期：2011-10-01 00:00:00

abstract：:Over the years, imino proton exchange measurements have provided a description of base-pair opening and of properties of internal motions of nucleic acids such as the individual opening of base pairs and the insensitivity of base-pair lifetimes to the nature and stability of neighboring pairs. A recent determination o...

journal_title：Biochimie

authors： Leijon M,Leroy JL

更新日期：1997-12-01 00:00:00

abstract：:Ligation of CD47 by its natural ligand thrombospondin (TSP), or cross-linking by CD47 antibodies, triggers caspase-independent cell death in normal and leukemic cells. This kind of cell death is characterised by the cytoplasmic events of apoptosis including externalisation of phosphatidylserines and mitochondria swell...

journal_title：Biochimie

authors： Roué G,Bitton N,Yuste VJ,Montange T,Rubio M,Dessauge F,Delettre C,Merle-Béral H,Sarfati M,Susin SA

更新日期：2003-08-01 00:00:00