Abstract：
:We identified a T-to-C mutation 2 nucleotides (nt) upstream from the AG in a GT-AG intron between exons 2 and 3 in the human T-cell leukemia virus type I (HTLV-I) tax mRNA. This mutation resulted in the preferential usage of an alternative splice site, causing a 75-nt elongation of tax mRNA and reduced production of viral antigens. When the clone containing this T-to-C mutation was reverted to the wild-type (T) DNA sequence, normal splicing of tax mRNA ensued and viral production was restored. These results suggest that the nucleotide at the position 2nt upstream from the AG in a GT-AG intron is important for the proper splicing of the HTLV-I tax gene, although it is not considered important for splicing in eukaryotes.
journal_name
Biochimiejournal_title
Biochimieauthors
Ohsugi Tdoi
10.1016/j.biochi.2005.07.013keywords:
subject
Has Abstractpub_date
2006-02-01 00:00:00pages
147-50issue
2eissn
0300-9084issn
1638-6183pii
S0300-9084(05)00191-4journal_volume
88pub_type
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