Abstract：
:Plant serine proteases have been widely used in food science and technology as well as in medicine. In this sense, several plant serine proteases have been proposed as potential anti-coagulants and anti-platelet agents. Previously, we have reported the purification and identification of a plant serine protease from Solanum tuberosum leaves. This potato enzyme, named as StSBTc-3, has a molecular weight of 72 kDa and it was characterized as a subtilisin like protease. In this work we determine and characterize the biochemical and medicinal properties of StSBTc-3. Results obtained show that, like the reported to other plant serine proteases, StSBTc-3 is able to degrade all chains of human fibrinogen and to produces fibrin clot lysis in a dose dependent manner. The enzyme efficiently hydrolyzes β subunit followed by partially hydrolyzed α and γ subunits of human fibrinogen. Assays performed to determine StSBTc-3 substrate specificity using oxidized insulin β-chain as substrate, show seven cleavage sites: Asn3-Gln4; Cys7-Gly8; Glu13-Ala14; Leu15-Tyr16; Tyr16-Leu17; Arg22-Gly23 and Phe25-Tyr26, all of them were previously reported for other serine proteases with fibrinogenolytic activity. The maximum StSBTc-3 fibrinogenolytic activity was determined at pH 8.0 and at 37 C. Additionally, we demonstrate that StSBTc-3 is able to inhibit platelet aggregation and is unable to exert cytotoxic activity on human erythrocytes in vitro at all concentrations assayed. These results suggest that StSBTc-3 could be evaluated as a new agent to be used in the treatment of thromboembolic disorders such as strokes, pulmonary embolism and deep vein thrombosis.
journal_name
Biochimiejournal_title
Biochimieauthors
Pepe A,Frey ME,Muñoz F,Fernández MB,Pedraza A,Galbán G,García DN,Daleo GR,Guevara MGdoi
10.1016/j.biochi.2016.03.015subject
Has Abstractpub_date
2016-06-01 00:00:00pages
163-70eissn
0300-9084issn
1638-6183pii
S0300-9084(16)30042-6journal_volume
125pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Trypsin and pronase treatment of purified human neutral bronchial mucins released small fragments from the C-terminal end of these molecules and resulted in slight increases in their sedimentation coefficient presumably reflecting conformational changes. The antigenic determinant of neutral bronchial mucins which appe...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80444-0
更新日期：1976-01-01 00:00:00
abstract：:The use of electrospun scaffolds for neural tissue engineering applications allows a closer mimicry of the native tissue extracellular matrix (ECM), important for the transplantation of cells in vivo. Moreover, the role of the electrospun fiber mat topography on neural stem cell (NSC) differentiation remains to be com...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2021.01.001
更新日期：2021-01-07 00:00:00
abstract：:Berardinelli-Seip congenital lipodystrophy (BSCL) is a rare recessive disease characterized by near absence of adipose tissue and severe insulin resistance. In most cases, BSCL is due to loss-of-function mutations in the genes encoding either seipin of unknown function or 1-acyl-glycerol-3-phosphate O-acyltransferase ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2009.01.011
更新日期：2009-06-01 00:00:00
abstract：:Thraustochytrium is a marine protist producing a specific profile of nutritionally important fatty acids, including very long chain polyunsaturated fatty acids (VLCPUFAs) docosahexaenoic acid (DHA, 22:6n-3), even chain saturated fatty acids (SFAs) palmitic acid (16:0), and odd chain SFAs pentadecanoic acid (15:0). To ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.10.024
更新日期：2018-01-01 00:00:00
abstract：:IL-28 and IL-29 were recently described as members of a new cytokine family that shares with type I interferon (IFN) the same Jak/Stat signalling pathway driving expression of a common set of genes. Accordingly, they have been named IFN lambda. IFNs lambda exhibit several common features with type I IFNs: antiviral ac...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2007.01.008
更新日期：2007-06-01 00:00:00
abstract：:The Gag-encoded nucleocapsid protein NCp7 (72 amino acids) from HIV-1, the regulatory protein, Vpr (96 amino acids) and numerous derivatives have been synthesized by solid phase method and their structures determined by 2D NMR. In NCp7, the two highly folded zinc fingers of the Cx2Cx4Hx4C type are in close spacial pro...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(97)83501-8
更新日期：1997-11-01 00:00:00
abstract：:IbpA/B, 16 kDa heat-shock proteins were recently described as recognizing heterologous protein inclusion bodies in Escherichia coli cells; the corresponding genes formed an operon regulated by the rpoH gene product, sigma 32 protein (Burland et al (1993) Genomics 16, 551; Allen et al (1992) J Bacteriol 174, 6938; Chua...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(96)82643-5
更新日期：1996-01-01 00:00:00
abstract：:We have proposed an allosteric ATP inhibition mechanism of Pfk-2 determining the structure of different forms of the enzyme together with a kinetic enzyme analysis. Here we complement the mechanism by using hybrid oligomers of the homodimeric enzyme to get insights about the allosteric communication pathways between t...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2016.08.013
更新日期：2016-09-01 00:00:00
abstract：:Isovaleryl-CoA dehydrogenase (IVD) catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA and the transfer of electrons to the electron transfer flavoprotein (ETF). Recombinant human IVD purifies with bound CoA-persulfide. A modified purification protocol was developed to isolate IVD without bound CoA-pers...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.11.007
更新日期：2015-01-01 00:00:00
abstract：:A large part of higher eukaryotic genomes is transcribed into RNAs lacking any significant open reading frame. This "non-coding part" has been shown to actively contribute to regulating gene expression, but the mechanisms are largely unknown. Particularly instructive examples are provided by the dosage compensation sy...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2011.12.026
更新日期：2012-07-01 00:00:00
abstract：:Glucose-6 phosphatase (G6Pase), a key enzyme of glucose homeostasis, catalyses the hydrolysis of glucose-6 phosphate (G6P) to glucose and inorganic phosphate. A deficiency in G6Pase activity causes type 1 glycogen storage disease (GSD-1), mainly characterised by hypoglycaemia. Genetic analyses of the two forms of this...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.09.022
更新日期：2012-03-01 00:00:00
abstract：:Adrenoleukodystrophy (ALD) is an X-linked peroxisomal disorder characterized by a progressive demyelination of the central nervous system and adrenal insufficiency. Clinical phenotypes of different severity are frequently observed within the same kindred. ALD is characterized biochemically by the accumulation of very-...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(93)90089-b
更新日期：1993-01-01 00:00:00
abstract：:A variety of transcription attenuation mechanisms are used by bacteria to regulate gene and operon expression. This review summarizes previous and current studies designed to elucidate the features of the specific attenuation mechanisms that regulate expression of the tryptophanase (tna) operon of Escherichia coli and...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(97)86725-9
更新日期：1996-01-01 00:00:00
abstract：:Calprotectin (CP) is an abundant protein in human neutrophilic granulocytes and macrophages. In humans, serum, urine, and fecal concentrations of neutrophil-derived proteins, such as CP are used as markers of disease activity for conditions associated with increased neutrophil activity, such as inflammatory bowel dise...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2008.03.008
更新日期：2008-09-01 00:00:00
abstract：:Parkinson's disease (PD), a neurodegenerative disorder, is characterized by a loss of dopaminergic neurons in the substantia nigra (SN) of the brain and it is well known that the pathogenesis of PD is related to a number of risk factors including oxidative stress. Antioxidant 1 (ATOX1) protein plays a crucial role in ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.10.010
更新日期：2019-01-01 00:00:00
abstract：:Split luciferase complementary assay has been used to investigate the effect of WD domain deletion on Apaf-1 oligomerization. Apaf-1 is an adaptor molecule in formation of apoptosome that activates caspase-9, an activation that is a key event in the mitochondrial cell death pathway. Structural studies suggest that nor...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.10.013
更新日期：2021-01-01 00:00:00
abstract：:The lignan 8,8'-bis-(methylenedioxy)cinnamic acid (BMDCA) is a powerful competitive inhibitor (K1 = 2.0 microM) of the lignin peroxidase (LiP) from Phanerochaete chrysosporium and of the extracellular peroxidase of Phlebia radiata (I0.5 = 10 microM). BMDCA derivatives with the same double bond system also inhibited th...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(96)88186-7
更新日期：1995-01-01 00:00:00
abstract：:Stroke is a worldwide major cause of mortality and morbidity. Preclinical studies have identified over 1000 molecules with brain-protective properties. More than 200 clinical trials have evaluated neuroprotective candidates for ischemic stroke yet, to date almost all failed, leading to a re-analysis of treatment strat...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.06.005
更新日期：2016-01-01 00:00:00
abstract：:The inhibition rates and spectral characteristics of 2 probes specific for the active-site serine residue of proteases were examined for evidence of conformational change of the proteases upon their binding to alpha 2-macroglobulin (alpha 2M). Elastase, chymotrypsin, trypsin, and plasmin were reacted with (7-nitrobenz...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(89)90004-7
更新日期：1989-03-01 00:00:00
abstract：:rBmTI-A is a recombinant serine protease inhibitor that belongs to the Kunitz-BPTI family and that was cloned from Rhipicephalus microplus tick. rBmTI-A has inhibitory activities on bovine trypsin, human plasma kallikrein, human neutrophil elastase and plasmin with dissociation constants in nM range. It is characteriz...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.12.014
更新日期：2020-12-24 00:00:00
abstract：:Peptidase family S46 consists of two types of dipeptidyl-peptidases (DPPs), DPP7 and DPP11, which liberate dipeptides from the N-termini of polypeptides along with the penultimate hydrophobic and acidic residues, respectively. Their specificities are primarily defined by a single amino acid residue, Gly673 in DPP7 and...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.10.015
更新日期：2018-04-01 00:00:00
abstract：:Glycogen was described as a temporal storage molecule in rhodococci, interconnecting lipids and carbon availability. The Rhodococcus jostii ADP-glucose pyrophosphorylase (ADP-GlcPPase) kinetic and regulatory properties support this role. Curiously, the enzyme uses glucosamine-1P as alternative substrate. Herein, we re...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.07.006
更新日期：2020-09-01 00:00:00
abstract：:High plasma choline has been associated with the metabolic syndrome and risk of chronic diseases, including cardiovascular disease. However, dietary choline is not correlated with choline plasma concentrations, and there are few studies and contradictory evidence regarding dietary choline and cardiovascular events. In...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.11.001
更新日期：2020-06-01 00:00:00
abstract：:The effect of curing agents (salt, nitrate, ascorbic acid and glucose) and processing parameters (pH, water activity and drying and cooking temperatures) on pork muscle cathepsins B, D, H and L as well as leucyl, arginyl and tyrosyl hydrolysing activities is reported. Salt (60 g/l) showed a powerful inhibitory effect,...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(92)90128-2
更新日期：1992-03-01 00:00:00
abstract：:Sm and Sm-like (Lsm) proteins are considered as an evolutionary conserved family involved in RNA metabolism in organisms from bacteria and archaea to human. Currently, the function of Sm-like archaeal proteins (SmAP) is not well understood. Here, we report the crystal structures of SmAP proteins from Sulfolobus acidoc...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.05.001
更新日期：2020-08-01 00:00:00
abstract：:NADH cytochrome b5 oxidoreductase (Ncb5or) protects β-cells against oxidative stress and lipotoxicity. The predominant phenotype of lean Ncb5or-null mouse is insulin-dependent diabetes due to β-cell death. This suggests the putative role of NCB5OR polymorphism in human diabetes. Therefore, we aimed to investigate the ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.03.004
更新日期：2013-07-01 00:00:00
abstract：:The present study has employed a combination of spectroscopic, calorimetric and computational methods to explore the binding of the three side-chained triazatruxene derivative, termed azatrux, to a human telomeric G-quadruplex sequence, under conditions of molecular crowding. The binding of azatrux to the tetramolecul...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.05.017
更新日期：2011-08-01 00:00:00
abstract：:We extracted from L. biflexa patoc a fraction F, reacting in hemagglutination and ring tests with sera prepared against more than ten different serogroups. This fraction contains mainly a polysaccharide (65 per cent), the role of which was clearly demonstrated in the precipitation reaction with homologous antisera, th...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80313-6
更新日期：1976-01-01 00:00:00
abstract：:The class I glutamine (Gln) tRNA synthetase interacts with the anticodon and acceptor stem of glutamine tRNA. RNA hairpin helices were designed to probe acceptor stem and anticodon stem-loop contacts. A seven-base pair RNA microhelix derived from the acceptor stem of tRNA(Gln) was aminoacylated by Gln tRNA synthetase....
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(93)90003-b
更新日期：1993-01-01 00:00:00
abstract：:The considerable affinity of tissue transglutaminase for heparin was the basis for use of heparin-based affinity matrices for enzyme purification. Interaction of transglutaminase with heparin might mimic the physiological binding to membrane heparan sulfates, accounting for the limited but significant fraction of enzy...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.01.012
更新日期：2005-06-01 00:00:00
