Abstract：
:Glycogen was described as a temporal storage molecule in rhodococci, interconnecting lipids and carbon availability. The Rhodococcus jostii ADP-glucose pyrophosphorylase (ADP-GlcPPase) kinetic and regulatory properties support this role. Curiously, the enzyme uses glucosamine-1P as alternative substrate. Herein, we report the in-depth study of glucosamine-1P activity and its regulation in two rhodocoocal ADP-GlcPPases, finding that glucosamine-6P (representing a metabolic carbon/nitrogen node) is a critical activator, then reinforcing the role of glycogen as an "intermediary metabolite" in rhodococci. Glucosamine-1P activity in rhodococcal ADP-GlcPPases responds to activation by metabolites improving their catalytic performance, strongly suggesting its metabolic feasibility. This work supports a scenario for new molecules/metabolites discovery and hypothesizes on evolutionary mechanisms underlying enzyme promiscuity opening novel metabolic features in (actino)bacteria.
journal_name
Biochimiejournal_title
Biochimieauthors
Cereijo AE,Alvarez HM,Iglesias AA,Asencion Diez MDdoi
10.1016/j.biochi.2020.07.006subject
Has Abstractpub_date
2020-09-01 00:00:00pages
158-161eissn
0300-9084issn
1638-6183pii
S0300-9084(20)30160-7journal_volume
176pub_type
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