Abstract：
:We previously reported that the rRNA methyltransferases RsmI and RsmH, which are responsible for cytidine dimethylation at position 1402 of 16S rRNA in the decoding center of the ribosome, contribute to Staphylococcus aureus virulence. Here we evaluated other 16S rRNA methyltransferases, including KsgA (RsmA), RsmB/F, RsmC, RsmD, RsmE, and RsmG. Knockout of KsgA, which methylates two adjacent adenosines at positions 1518 and 1519 of 16S rRNA in the intersubunit bridge of the ribosome, attenuated the S. aureus killing ability against silkworms. The ksgA knockout strain was sensitive to oxidative stress and had a lower survival rate in murine macrophages than the parent strain. The ksgA knockout strain exhibited decreased translational fidelity in oxidative stress conditions. Administration of N-acetyl-l-cysteine, a free-radical scavenger, restored the killing ability of the ksgA knockout strain against silkworms. These findings suggest that the methyl-modifications of 16S rRNA by KsgA contribute to maintain ribosome function under oxidative conditions and thus to S. aureus virulence.
journal_name
Biochimiejournal_title
Biochimieauthors
Kyuma T,Kizaki H,Ryuno H,Sekimizu K,Kaito Cdoi
10.1016/j.biochi.2015.10.027subject
Has Abstractpub_date
2015-12-01 00:00:00pages
166-74eissn
0300-9084issn
1638-6183pii
S0300-9084(15)00347-8journal_volume
119pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Vegetable oils, consisting principally of triacylglycerols (TAG), are major sources of calories and essential fatty acids in the human diet. The fatty acid composition of TAG is a primary determinant of the nutritional quality and health-promoting properties of vegetable oils. TAG fatty acid composition also affects t...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2020.09.020
更新日期：2020-11-01 00:00:00
abstract：:Berardinelli-Seip congenital lipodystrophy (BSCL) is a rare recessive disease characterized by near absence of adipose tissue and severe insulin resistance. In most cases, BSCL is due to loss-of-function mutations in the genes encoding either seipin of unknown function or 1-acyl-glycerol-3-phosphate O-acyltransferase ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2009.01.011
更新日期：2009-06-01 00:00:00
abstract：:After 30 years of effort, the mammalian adenylate cyclase catalytic subunit has now been purified. It is a glycoprotein of 155 kDa, representing less than 0.01% of synaptosomal membrane protein. As measured in the presence of forskolin, its specific activity is 10-20 mumol of cAMP X mg-1 X min-1. The enzyme obtained i...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(87)90016-2
更新日期：1987-04-01 00:00:00
abstract：:Plant sucrose transporters (SUTs) are functional as sucrose-proton-cotransporters with an optimal transport activity in the acidic pH range. Recently, the pH optimum of the Solanum tuberosum sucrose transporter StSUT1 was experimentally determined to range at an unexpectedly low pH of 3 or even below. Various research...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.07.028
更新日期：2013-11-01 00:00:00
abstract：:Triiodothyronine (T3), injected daily into rats, induces heart hypertrophy. In a recent work we have shown that a single T3 injection stimulates RNA synthesis and modifies the translational products of myocardial mRNAs in reticulocyte lysates, quantitatively and qualitatively. In this work we show that T3 induces smal...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(84)90113-5
更新日期：1984-09-01 00:00:00
abstract：:Structural studies on various electron transfer complexes involving the tetrahemic cytochrome c3 provided evidence that one of the hemes (heme 4) is the interacting site on the molecule. The reactivity of this particular heme is allocated to the positive charges found around the heme group which are strongly involved ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(94)90175-9
更新日期：1994-01-01 00:00:00
abstract：:We have analyzed changes in the supercoiling of nucleoid DNA of murine fibroblasts cultured in the presence of hydralazine. The entire DNA attached to the nuclear matrix was extracted from the cells and sedimented in neutral sucrose density gradients containing ethidium bromide. Nucleoids from cells treated with hydra...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(98)80025-4
更新日期：1998-07-01 00:00:00
abstract：:Chagasin-type inhibitors comprise natural inhibitors of papain-like cysteine proteases that are distributed among Protist, Bacteria and Archaea. Chagasin was identified in the pathogenic protozoa Trypanosoma cruzi as an approximately 11 kDa protein that is a tight-binding and highly thermostable inhibitor of papain, c...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.11.002
更新日期：2016-03-01 00:00:00
abstract：:Specific contacts between DNA phosphate groups and positively charged nucleophilic amino acids from the Escherichia coli Fpg protein play a significant role in DNA-Fpg protein interaction. In order to identify these phosphate groups the chemical crosslinking procedure was carried out. The probing of the Fpg protein ac...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(03)00067-1
更新日期：2003-05-01 00:00:00
abstract：:Polysialylated neural cell adhesion molecule (NCAM) was immunoaffinity-purified from the brains of newborn calves. A degree of polymerization of up to 40 was chromatographically determined for released polysialic acid (PSA) chains. For characterization of N-glycan structures and attachment sites, PSA-NCAM was digested...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(03)00043-9
更新日期：2003-01-01 00:00:00
abstract：:The eosinophil cationic protein (ECP) is a human antimicrobial protein involved in the host immune defense that belongs to the pancreatic RNase A family. ECP displays a wide range of antipathogen activities. The protein is highly cationic and its bactericidal activity is dependant on both cationic and hydrophobic surf...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.10.005
更新日期：2011-02-01 00:00:00
abstract：:Many peptide hormone and neuropeptide precursors undergo post-translational processing at mono- and/or dibasic residues. An enzymatic activity capable of processing prodynorphin at a monobasic processing site designated 'dynorphin converting enzyme' has been previously reported in rat rain and bovine pituitary. In thi...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(94)90153-8
更新日期：1994-01-01 00:00:00
abstract：:Viral expression was analyzed in ten cell clones of a Friend erythroleukemia cell line (HFL/b cell line [3]), which had lost its capacity to produce infectious particles. All the ten subclones were non producers but expressed spleen focus forming virus (SFFV) polypeptides in the form of p48-p50gag and gp50-gp52env. On...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(83)80277-6
更新日期：1983-04-01 00:00:00
abstract：:Mouse brain myelin consists of a continuum of particles of different densities, as shown by sucrose density gradient centrifugation. In normal animals most of the material (65 per cent) is concentrated between 0.6 and 0.7 M sucrose (the maximum being found at 0.66 M sucrose, corresponding to 23 per cent). The density ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(77)80213-7
更新日期：1977-01-01 00:00:00
abstract：:Anaerobiospirillum succiniciproducens phosphoenolpyruvate (PEP) carboxykinase catalyses the reversible metal-dependent formation of oxaloacetate (OAA) and ATP from PEP, ADP and CO(2). Mutations of PEP carboxykinase have been constructed where the residues His(225) and Asp(263), two residues of the enzyme's putative Mn...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2003.10.013
更新日期：2004-01-01 00:00:00
abstract：:Binding of indoloquinolines with different aliphatic side chains to a parallel G-quadruplex derived from the MYC promoter sequence was characterized by optical and calorimetric measurements. ITC experiments performed at different temperatures enabled the determination of molar heat capacity changes upon quadruplex bin...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.11.015
更新日期：2019-02-01 00:00:00
abstract：:In 1956, when we started our collaboration, both Alick Isaacs and myself had done previous work on interference between inactive and active influenza viruses. We were aware of the state interference research had reached and of the two alternative explanations that had been envisaged. ...
journal_title：Biochimie
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.biochi.2007.04.007
更新日期：2007-06-01 00:00:00
abstract：:The targets of in vivo studies of the ribosomal assembly process are mainly the events of rRNA processing, whereas in vitro studies (total reconstitution) focus on principles of the assembly process such as assembly-initiation proteins, rate-limiting steps and a detailed sequence of assembly reactions (assembly map). ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(91)90054-5
更新日期：1991-06-01 00:00:00
abstract：:Fluorescence techniques were used to detect changes in the conformation of tRNA(Phe) that may occur during the peptidyl transferase reaction in which the tRNA appears to move between binding sites on ribosomes. Such a conformational change may be a fundamental part of the translocation mechanism by which tRNA and mRNA...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(87)90226-4
更新日期：1987-09-01 00:00:00
abstract：:Cell walls of flocculent strains (0006) and non flocculent strains (0019) of Saccharomyces uvarum (Carlsbergensis), grown in different media and taken in both growth and stationary phases, were treated with water and with 2 per cent (W/V) potassium hydroxide. This treatment yielded four fractions (FI, FII, FIII and FR...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(81)80055-7
更新日期：1981-07-01 00:00:00
abstract：:Snake venom phospholipases A2 (PLA2) share high sequence identities and a conserved structural scaffold, but show important functional differences. Only a few PLA2s have been purified and characterized from coral snake (Micrurus spp.) venoms, and their role in envenomation remains largely unknown. In this report, we d...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.03.008
更新日期：2017-06-01 00:00:00
abstract：:DNA replication in Escherichia coli is controlled at the initiation stage, possibly by regulation of the essential activity of DnaA protein. The cellular membrane has long been hypothesized to be involved in chromosomal replication. Accumulating evidence, both in vitro and in vivo, that supports the importance of memb...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(00)01224-4
更新日期：2001-01-01 00:00:00
abstract：:Reuber H 35 hepatoma cells were synchronized by transfer in a serum free medium. Growth was re-initiated by addition of serum. Under these conditions DNA synthesis exhibited a maximum after 24 hours. Chromatin non-histone proteins prepared from cells at various phases of the cell cycle were incubated with [gamma-32P] ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(79)80280-1
更新日期：1979-01-01 00:00:00
abstract：:Thyroglobulin is a large-size iodoglycoprotein specific to thyroid tissue and is the substrate for the synthesis of thyroid hormones, thyroxine and 3,5,3'-triiodothyronine. Recent studies, which greatly benefited from recombinant DNA methodologies, improved the knowledge of several structural features of this dimeric ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(89)90057-6
更新日期：1989-02-01 00:00:00
abstract：:Fluorometry using a substrate DNA labeled with a single fluorophore (6-carboxyfluorescein) at the 3'-end of the processed strand was shown to be a useful tool for monitoring DNA-binding and 3'-processing activities of HIV-1 and PFV integrases (INs). The DNA binding to either of the INs resulted in a fluorescence signa...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.06.009
更新日期：2012-11-01 00:00:00
abstract：:By use of procollagen proline hydroxylas which catalyses the substitution of the hydrogen in the trans position on carbon 4 with an hydroxyl on L-proline residues of procollagen, we have shown that the L-[3H-4] proline product of C.E.N. Saclay contained 29 per cent of the trans and 71 per cent of the cis form. It was ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80433-6
更新日期：1976-01-01 00:00:00
abstract：:Rhizopus oryzae lipase (ROL) was found to be a true lipase. This enzyme presents the interfacial activation phenomenon. The N-terminal amino acid sequence of ROL was compared to those of rhizopus lipases. Purified ROL possesses the same N-terminal sequence as the mature Rhizopus niveus lipase (RNL). This sequence was ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(01)01283-4
更新日期：2001-06-01 00:00:00
abstract：:The product of the spoIIM gene of Bacillus subtilis is required for complete septum migration and forespore engulfment during sporulation. To investigate whether expression of spoIIM is required in the forespore compartment of the sporangium, we have constructed a new integrational vector, pKSV7, which contains temper...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(92)90143-3
更新日期：1992-07-01 00:00:00
abstract：:We are reporting here an analysis of the chymotryptic peptides obtained from the tryptic heme-binding fragment of flavocytochrome b2 (cytochrome b2 core). These results completely establish the sequence of the 96 residue-long fragment, for which preliminary evidence has been published before [24]. We also report full ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80437-3
更新日期：1976-01-01 00:00:00
abstract：:Direct demonstration of the reversible dissociation of native dimeric methionyl-tRNA synthetase from E. coli has been obtained using small angle neutron scattering and deuterated enzyme. Structural parameters of the fully deuterated dimer are very similar to the hydrogenated one. Analysis of the variations of the inte...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80205-4
更新日期：1985-06-01 00:00:00
