Isolation and characterization of a gp 70+ non producer Friend tumor cell clone.

abstract：:The chemical carcinogen N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) induces frameshift mutations located within two types of specific sequences (mutation hot spots): i) contiguous guanine sequences and ii) alternating GC sequences. The genetic requirements of these frameshift events were investigated using specific ...

journal_title：Biochimie

authors： Janel-Bintz R,Maenhaut-Michel G,Takahashi M,Fuchs RP

更新日期：1991-04-01 00:00:00

abstract：:Estrogen plays an essential role in bone formation, and estrogen modulation dysfunction is tightly associated with postmenopausal osteoporosis (PMOP). The underlying mechanisms of estrogen-mediated osteogenic differentiation have not been well defined. In this study, murine bone marrow mesenchymal stem cells were indu...

journal_title：Biochimie

authors： Gao B,Huang Q,Jie Q,Wang L,Zhang HY,Liu J,Yang L,Luo ZJ

更新日期：2015-03-01 00:00:00

abstract：:The very end of the chromosome is called the telomere and is composed of DNA repeat sequences and associated proteins. Genetic and biochemical analyses of this complex, the telosome, lead to the hypothesis that transcription and DNA replication are submitted to position effects mediated by the telomere proximity. Telo...

journal_title：Biochimie

authors： Pommier JP,Lebeau J,Ducray C,Sabatier L

更新日期：1995-01-01 00:00:00

abstract：:Lactic acid bacteria provide a model system for the in vivo study of mechanisms pertaining to the regulation of sugar transport and metabolism by microorganisms. Recent studies with resting and growing cells of the homofermentative Streptococci and Lactobacilli have yielded evidence for hitherto unsuspected regulatory...

journal_title：Biochimie

authors： Thompson J

更新日期：1988-03-01 00:00:00

abstract：:Thyroid peroxidase (TPO) is the key enzyme involved in thyroid hormone synthesis. Autoantibodies to TPO (TPOAbs) are a hallmark of autoimmune thyroid disease (AITD). Here, we highlight recent progress over several years in understanding TPO biochemistry and function in various pathologies. TPO undergoes complex post-t...

journal_title：Biochimie

authors： Godlewska M,Banga PJ

更新日期：2019-05-01 00:00:00

abstract：:Cell-to-cell movement of Poa semilatent virus (genus Hordeivirus) in infected plants is mediated by three viral 'triple gene block' (TGB) proteins. One of those termed TGBp3 is an integral membrane protein essential for intracellular transport of other TGB proteins and viral genomic RNA to plasmodesmata. TGBp3 targeti...

journal_title：Biochimie

authors： Shemyakina EA,Erokhina TN,Gorshkova EN,Schiemann J,Solovyev AG,Morozov SY

更新日期：2011-04-01 00:00:00

abstract：:Azelaic acid (AzA), a C9 linear α,ω-dicarboxylic acid, is found in whole grains namely wheat, rye, barley, oat seeds and sorghum. The study was performed to investigate whether AzA exerts beneficial effect on hepatic key enzymes of carbohydrate metabolism in high fat diet (HFD) induced type 2 diabetic C57BL/6J mice. C...

journal_title：Biochimie

authors： Muthulakshmi S,Saravanan R

更新日期：2013-06-01 00:00:00

abstract：:The presence of a fragment of polyA resistant to both T1 and p ribonucleases in mRNAs extracted from wing imaginal disks of an insect, Pieris brassicae, is reported. Its length was approximatively 150 nucleotides. PolyU sepharose affinity chromatography was subsequently used for purification of these polyA(+)mRNA mole...

journal_title：Biochimie

authors： Tarroux P

更新日期：1975-01-01 00:00:00

abstract：:Pseudouridine [Ψ] is a frequent base modification in the ribosomal RNA [rRNA] and may be involved in the modulation of the conformational flexibility of rRNA helix-loop structures during protein synthesis. Helix 69 of 23S rRNA contains pseudouridines at the positions 1911, 1915 and 1917 which are formed by the helix 6...

journal_title：Biochimie

authors： Kipper K,Sild S,Hetényi C,Remme J,Liiv A

更新日期：2011-05-01 00:00:00

abstract：:The functions of residues 57-RY-58, G60, L77, 80-GSGR-83, I101, T104, 134-GY-135, N155, V157 and 160-LV-161 in human arsenic (III) methyltransferase (hAS3MT) 5 Å around S-adenosylmethionine (SAM) have not been studied. Herein, sixteen mutants were designed by substituting these residues with Ala. Mutants G60A, G80A, I...

journal_title：Biochimie

authors： Li X,Geng Z,Chang J,Song X,Wang Z

更新日期：2014-12-01 00:00:00

abstract：:The nucleotide sequence of the gene encoding chicken ribosomal protein L7a was determined. The gene contains eight exons and seven introns, which spread over 3613 nucleotides. The transcription initiation sites were located on four consecutive nucleotides GCCC, which are at the 5' terminus of a short polypyrimidine tr...

journal_title：Biochimie

authors： Maeda N,Kenmochi N,Tanaka T

更新日期：1993-01-01 00:00:00

abstract：:A lignin model compound, named in short guaiagylglycerol beta-guaiacyl ether (GGE), contains the beta-0-4 ether linkage that is common in the chemical structure of lignin. A Pseudomonas sp. (GU5) had been isolated as an organism able to grow with GGE as the sole source of carbon and energy. When grown on vanillate, th...

journal_title：Biochimie

authors： Pelmont J,Barrelle M,Hauteville M,Gamba D,Romdhane M,Dardas A,Beguin C

更新日期：1985-09-01 00:00:00

abstract：:Human mitochondrial diseases, defined as the diseases due to a mitochondrial oxidative phosphorylation defect, represent a large group of very diverse diseases with respect to phenotype and genetic causes. They present with many unsolved issues, the comprehensive analysis of which is beyond the scope of this review. W...

journal_title：Biochimie

authors： Lombès A,Auré K,Bellanné-Chantelot C,Gilleron M,Jardel C

更新日期：2014-05-01 00:00:00

abstract：:Tri(n-butyl)phosphate (TNBP) and sodium cholate (SC) mixtures have been used to inactivate lipid-enveloped viruses like HIV and hepatitis B. We exploited the use of this combination to purify fibroblast growth factor-2 (FGF-2) from human placenta. Human placentas were extracted in the presence of 0.3% TNBP/0.2% SC and...

journal_title：Biochimie

authors： Wolff P,Tanaka AM,Chenker E,Cabrera-Crespo J,Raw I,Ho PL

更新日期：1996-01-01 00:00:00

abstract：:Porphyromonas gingivalis uses a type IX secretion system (T9SS) to deliver more than 30 proteins to the bacterial surface using a conserved C-terminal domain (CTD) as an outer membrane translocation signal. On the surface, the CTD is cleaved and an anionic lipopolysaccharide (A-PLS) is attached by PorU sortase. Among ...

journal_title：Biochimie

authors： Veillard F,Sztukowska M,Nowakowska Z,Mizgalska D,Thøgersen IB,Enghild JJ,Bogyo M,Potempa B,Nguyen KA,Potempa J

更新日期：2019-11-01 00:00:00

abstract：:Peroxisomes are subcellular organelles which are present in virtually every eukaryotic cell and catalyze a large number of metabolic functions. The importance of peroxisomes for humans is stressed by the existence of a large group of genetic diseases in which either the biogenesis of peroxisomes is impaired or one of ...

journal_title：Biochimie

authors： Wanders RJ

更新日期：2014-03-01 00:00:00

abstract：:Ferritins are ubiquitous iron-storage proteins found in all kingdoms of life. They share a common architecture made of 24 subunits of five α-helices. The recombinant Chlorobium tepidum ferritin (rCtFtn) is a structurally interesting protein since sequence alignments with other ferritins show that this protein has a si...

journal_title：Biochimie

authors： Arenas-Salinas M,Townsend PD,Brito C,Marquez V,Marabolli V,Gonzalez-Nilo F,Matias C,Watt RK,López-Castro JD,Domínguez-Vera J,Pohl E,Yévenes A

更新日期：2014-11-01 00:00:00

abstract：:Honeybee-plant relationships are based on a conditioning process in which olfactory (plant aroma) and gustatory cues (mainly nectars) are closely linked, leading to a selective foraging behaviour. Among crops dependent upon entomophilous cross-pollination, the sunflower has recently undergone extensive expansion due t...

journal_title：Biochimie

authors： Pham-Delegue MH,Etievant P,Masson C

更新日期：1987-06-01 00:00:00

abstract：:The nuclear metabolism of poly(ADP-ribose) is mainly regulated by poly(ADP-ribose) polymerase-1 (PARP-1) and by poly(ADP-ribose) glycohydrolase (PARG). A PARP-like enzyme, V-PARP, and a PARG isoform are present in the extra-nuclear compartment of mammalian cells, even if poly(ADP-ribose) has never been detected therei...

journal_title：Biochimie

authors： Rossi L,Denegri M,Torti M,Poirier GG,Ivana Scovassi A

更新日期：2002-12-01 00:00:00

abstract：:TcrX/Y is one of the twelve two component system (TCS) present in Mycobacterium tuberculosis. We have investigated the TcrX/Y interaction by in silico studies, pull down assay, radioactive phosphotransfer, surface plasmon resonance as well as crosstalk analysis of TcrY with TcrA - a non-cognate response regulator. Seq...

journal_title：Biochimie

authors： Bhattacharya M,Biswas A,Das AK

更新日期：2010-03-01 00:00:00

abstract：:Structural and motional features in the denatured state of a protein dictate the early folding events starting from that state and these features vary depending upon the nature of the denaturant used. Here, we have attempted to decipher the early events in the folding of Dynein Light Chain protein (DLC8), starting fro...

journal_title：Biochimie

authors： Chakraborty S,Mohan PM,Hosur RV

更新日期：2012-01-01 00:00:00

abstract：:Homocysteine (HCY) is a degradation product of the methionine pathway. The B vitamins, in particular vitamin B12 and folate, are the primary nutritional determinant of HCY levels and therefore their deficiencies result in hyperhomocysteinaemia (HHCY). Prevalence of hyperhomocysteinemia (HHCY) and related dietary defic...

journal_title：Biochimie

authors： Feigerlova E,Demarquet L,Guéant JL

更新日期：2016-07-01 00:00:00

abstract：:In recent years an increasing number of inherited decreases in man has been identified in which there is an impairment of one or more peroxisomal functions. Sofar 15 different peroxisomal disorders have been identified which can be subdivided into three distinct groups depending upon whether there is a generalized (gr...

journal_title：Biochimie

authors： Wanders RJ,Schutgens RB,Barth PG,Tager JM,van den Bosch H

更新日期：1993-01-01 00:00:00

abstract：:L-aromatic aminoacid decarboxylase has been purified more than thousand times from homogenates of rat brain, in several steps : centrifugation, DEAE-cellulose, CM cellulose, hydroxylapatite, DEAE sephadex. Its properties have been studied, most of them on an intermediate fraction of the purification, because of the in...

journal_title：Biochimie

authors： Corgier M,Pacheco H

更新日期：1975-01-01 00:00:00

abstract：:Murine L5178Y cell ribosomes were dissociated into subunits either with potassium chloride in the presence of puromycin or with the chelating agent EDTA. The proteins of ribosomal subunits obtained by these different methods were compared by means of bidimensional polyacrylamide gel electrophoresis. KCl-derived 60S an...

journal_title：Biochimie

authors： Longuet M,Auger-Buendia MA,Tavitian A

更新日期：1979-01-01 00:00:00

abstract：:The efficiency of oxidative phosphorylation was estimated in intact resting cells of Escherichia coli K 12, strain PA 601 (chl-s) and its chl-r mutants, all of them grown anaerobically in the presence of nitrate. The oxidation of endogenous NADH in intact chl-s cells was accompanied by the formation of ATP whatever th...

journal_title：Biochimie

authors： Giordano G,Rivière C,Azoulay E

更新日期：1977-01-01 00:00:00

abstract：:gamma-Aminobutyraldehyde dehydrogenase from Escherichia coli K-12 has been purified and characterized from cell mutants able to grow in putrescine as the sole carbon and nitrogen source. The enzyme has an Mr of 195,000 +/- 10,000 in its dimeric form with an Mr of 95,000 +/- 1,000 for each subunit, a pH optimum at 5.4 ...

journal_title：Biochimie

authors： Prieto MI,Martin J,Balaña-Fouce R,Garrido-Pertierra A

更新日期：1987-11-01 00:00:00

abstract：:Synthesis and secretion of polysaccharides by Gram-negative bacteria are a result of a concerted action of enzymatic and channel-forming proteins localized in different compartments of the cell. The presented work comprises functional characterization of PssO protein encoded within the previously identified, chromosom...

journal_title：Biochimie

authors： Marczak M,Mazur A,Gruszecki WI,Skorupska A

更新日期：2008-11-01 00:00:00

abstract：:Explants from 7, 8, 9, 11, 13-day chick embryonic skin incorporating (3H) Uridine for different periods 1 hr, 3 or 4 hr and a chase with actinomycin) are studied with respect to free (F) or membrane bound (B) cytoplasmic polysomes and to RNA extracted from them. Polysome specific activity decreases at older stages but...

journal_title：Biochimie

authors： Desveaux-Chabrol J

更新日期：1976-01-01 00:00:00

abstract：:This paper deals with the discovery of plasmalogen deficiency in the cerebro-hepato-renal (Zellweger) syndrome and discusses how this has led to the development of postnatal and prenatal diagnostic procedures for this and a number of related peroxisomal disorders in man that show a general impairment in the biosynthes...

journal_title：Biochimie

authors： van den Bosch H,Schrakamp G,Hardeman D,Zomer AW,Wanders RJ,Schutgens RB

更新日期：1993-01-01 00:00:00