Characterization and purification of messenger RNAs containing poly A in insect imaginal disks.

Abstract:

:The presence of a fragment of polyA resistant to both T1 and p ribonucleases in mRNAs extracted from wing imaginal disks of an insect, Pieris brassicae, is reported. Its length was approximatively 150 nucleotides. PolyU sepharose affinity chromatography was subsequently used for purification of these polyA(+)mRNA molecules. Analyses on sucrose gradients showed a good recovery of poly(+)molecules characterized by their size (20-100 S) and a polydisperse pattern. These mRNAlike species represent 2-3 per cent of the total radioactivity incorporated into RNA in 3 hours of labeling. Sequential extractions were carried out to provide cytoplasmic RNA rich fractions (4 degrees C) and nuclear rich fractions (45 degrees C). When assayed for the presence of polyA(+)RNA, molecules extracted by these two sequential methods were found to be very similar in their polyA content.

journal_name

Biochimie

journal_title

Biochimie

authors

Tarroux P

doi

10.1016/s0300-9084(75)80049-6

keywords:

subject

Has Abstract

pub_date

1975-01-01 00:00:00

pages

757-63

issue

6-7

eissn

0300-9084

issn

1638-6183

pii

S0300-9084(75)80049-6

journal_volume

57

pub_type

杂志文章
  • Purification and biochemical characterization of a lysosomal α-fucosidase from the deuterostomia Asterias rubens.

    abstract::In vertebrates, mannose 6-phosphate receptors [MPR300 (Mr 300 kDa) and MPR46 (Mr 46 kDa)] are highly conserved transmembrane glycoproteins that mediate transport of lysosomal enzymes to lysosomes. Our studies have revealed the appearance of these putative receptors in invertebrates such as the molluscs and deuterostom...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2012.02.007

    authors: Visa M,Hammer E,Völker U,Koliwer-Brandl H,Kelm S,Nadimpalli SK

    更新日期:2012-05-01 00:00:00

  • Degradation of tropoelastin and skin elastin by neprilysin.

    abstract::Neprilysin is also known as skin fibroblast-derived elastase, and its up-regulation during aging is associated with impairments of the elastic fiber network, loss of skin elasticity and wrinkle formation. However, information on its elastase activity is still limited. The aim of this study was to investigate the degra...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2017.11.018

    authors: Mora Huertas AC,Schmelzer CEH,Luise C,Sippl W,Pietzsch M,Hoehenwarter W,Heinz A

    更新日期:2018-03-01 00:00:00

  • Cyanidin-3-glucoside induces mesenchymal to epithelial transition via activating Sirt1 expression in triple negative breast cancer cells.

    abstract::Triple-negative breast cancer (TNBC) is a heterogeneous group of breast cancer with one common feature: distinctly metastatic nature with higher rate of relapse and shorter survival compared with other subtypes of breast cancer. The epithelial to mesenchymal transition (EMT) is highly associated with cancer metastasis...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2019.03.004

    authors: Liang L,Liu X,He J,Shao Y,Liu J,Wang Z,Xia L,Han T,Wu P

    更新日期:2019-07-01 00:00:00

  • Signaling pathways involved in thyroid hyperfunction and growth in Graves' disease.

    abstract::The elucidation of the multiple signaling cascades coupled to the TSH receptor has offered new approaches in the understanding of the pathogenesis of Graves' disease. Here we review findings showing that immunoglobulins from Graves' patients are heterogeneous, bind to different epitopes and, similarly to TSH, activate...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/s0300-9084(99)80090-x

    authors: Di Cerbo A,Corda D

    更新日期:1999-05-01 00:00:00

  • Lipidomic approaches to the study of phospholipase A2-regulated phospholipid fatty acid incorporation and remodeling.

    abstract::The distribution of fatty acids among cellular glycerophospholipids is finely regulated by the CoA-dependent acylation of lysophospholipids followed by transacylation reactions. Arachidonic acid is the fatty acid precursor of a wide family of bioactive compounds called the eicosanoids, with key roles in innate immunit...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2009.11.010

    authors: Balgoma D,Montero O,Balboa MA,Balsinde J

    更新日期:2010-06-01 00:00:00

  • Towards structural models of molecular recognition in olfactory receptors.

    abstract::The G protein coupled receptors (GPCR) are an important class of proteins that act as signal transducers through the cytoplasmic membrane. Understanding the structure and activation mechanism of these proteins is crucial for understanding many different aspects of cellular signalling. The olfactory receptors correspon...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/s0300-9084(98)80019-9

    authors: Afshar M,Hubbard RE,Demaille J

    更新日期:1998-02-01 00:00:00

  • From green chemistry to nature: The versatile role of low transition temperature mixtures.

    abstract::In 1998, the concept of "green chemistry" was established through twelve principles with the aim of improving the eco-efficiency of chemical processes and to judge, whether or not, a chemical process is sustainable. Currently, numerous processes do not obey to most of these principles (large energy usage, formation of...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2015.09.019

    authors: Durand E,Lecomte J,Villeneuve P

    更新日期:2016-01-01 00:00:00

  • Apolipoprotein A-I improves hepatic autophagy through the AMPK pathway.

    abstract::Dysfunction in lipid metabolism may result in a decrease in hepatic autophagy, which contributes to the pathogenesis of non-alcoholic steatohepatitis. ATP-binding cassette transporter A1 transports free cholesterol and phospholipids to apolipoprotein A-I (apoA-I) to form nascent high-density lipoprotein particles. Res...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2019.08.001

    authors: Rao X,Wang Y

    更新日期:2019-10-01 00:00:00

  • Cloning and molecular modelling of turkey pancreatic lipase: structural explanation of the increased interaction power with lipidic interface.

    abstract::Starting from total pancreatic mRNAs, turkey pancreatic lipase (TPL) cDNA was synthesized by RT-PCR and cloned into the PGEM-T vector. Amino acid sequence of the TPL is compared to that of human pancreatic lipase (HPL). A 3-D structure model of TPL was built using the 3-D structure of HPL as template, given the high a...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2006.05.022

    authors: Fendri A,Frikha F,Miled N,Gargouri Y

    更新日期:2006-10-01 00:00:00

  • GKN1 expression in gastric cancer cells is negatively regulated by miR-544a.

    abstract::Gastrokine1 (GKN1), important for maintaining the physiological function of the gastric mucosa, is highly expressed in the stomach of healthy individuals but is down-regulated or absent in gastric tumor tissues and derived cell lines. The mechanisms underlying GKN1 gene inactivation are still unknown. We previously sh...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2019.09.005

    authors: Stella di Stadio C,Faraonio R,Federico A,Altieri F,Rippa E,Arcari P

    更新日期:2019-12-01 00:00:00

  • 50 years ago: how prepared were our minds?

    abstract::In 1956, when we started our collaboration, both Alick Isaacs and myself had done previous work on interference between inactive and active influenza viruses. We were aware of the state interference research had reached and of the two alternative explanations that had been envisaged. ...

    journal_title:Biochimie

    pub_type: 历史文章,杂志文章,评审

    doi:10.1016/j.biochi.2007.04.007

    authors: Lindenmann J

    更新日期:2007-06-01 00:00:00

  • Properties of chromosomal proteins of human leukemic cells.

    abstract::Purified chromatin isolated from lymphocytic cells derived from patients with acute leukemia, or other lymphoproliferative disorders has been compared with chromatin isolated from normal human lymphocytic cells by gel electrophoresis and differential gradient ultracentrifugation. Thermal denaturation studies showed hi...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(75)80307-5

    authors: Desai LS,Wulff UC,Foley GE

    更新日期:1975-01-01 00:00:00

  • Crystal structure of dihydroorotate dehydrogenase from Leishmania major.

    abstract::Dihydroorotate dehydrogenase (DHODH) is the fourth enzyme in the de novo pyrimidine biosynthetic pathway and has been exploited as the target for therapy against proliferative and parasitic diseases. In this study, we report the crystal structures of DHODH from Leishmania major, the species of Leishmania associated wi...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2012.04.003

    authors: Cordeiro AT,Feliciano PR,Pinheiro MP,Nonato MC

    更新日期:2012-08-01 00:00:00

  • Effect of the thermal variation on the molecular weight estimation of the acid phosphatase from Physarum polycephalum.

    abstract::A Binding Protein (BP) of nearly 95,000 daltons interacts with the acid phosphatase at the permissive temperature, elevating its molecular weight from 48,000 +/- 2,000 to 136,000 +/- 7,000 in CL and CHR strains of Physarum polycephalum plasmodia. The same molecule has not been observed in the extracts of M3C VIII and ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(83)80160-6

    authors: Demirtas H

    更新日期:1983-06-01 00:00:00

  • Automatic identification of large collections of protein-coding or rRNA sequences.

    abstract::The number of available genomic sequences is growing very fast, due to the development of massive sequencing techniques. Sequence identification is needed and contributes to the assessment of gene and species evolutionary relationships. Automated bioinformatics tools are thus necessary to carry out these identificatio...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2007.08.006

    authors: Arigon AM,Perrière G,Gouy M

    更新日期:2008-04-01 00:00:00

  • c-Jun transcriptionally regulates alpha 1, 2-fucosyltransferase 1 (FUT1) in ovarian cancer.

    abstract::Alpha 1, 2-fucosyltransferase (FUT 1/2) is a rate-limiting enzyme that catalyzes the synthesis of Lewis y, a cell membrane-associated carbohydrate antigen. In human ovarian cancer, the upregulated expression of FUT1 and Lewis y is associated with advanced pathological stages and involved in cell proliferation, migrati...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2014.09.015

    authors: Gao N,Liu J,Liu D,Hao Y,Yan L,Ma Y,Zhuang H,Hu Z,Gao J,Yang Z,Shi H,Lin B

    更新日期:2014-12-01 00:00:00

  • Feedback control of ribosome function in Escherichia coli.

    abstract::We have previously proposed that the rate of ribosome function during balanced growth in E. coli, expressed as the rate of peptide chain elongation, is adjusted by a feedback mechanism: whenever that rate is submaximal (i.e. below 22 amino acid residues polymerized per active ribosome at 37 degrees C), the feedback si...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2007.10.008

    authors: Bremer H,Dennis P

    更新日期:2008-03-01 00:00:00

  • Potential role and therapeutic interests of myo-inositol in metabolic diseases.

    abstract::Several inositol isomers and in particular myo-inositol (MI) and D-chiro-inositol (DCI), were shown to possess insulin-mimetic properties and to be efficient in lowering post-prandial blood glucose. In addition, abnormalities in inositol metabolism are associated with insulin resistance and with long term microvascula...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2013.05.011

    authors: Croze ML,Soulage CO

    更新日期:2013-10-01 00:00:00

  • Heteroarene-fused anthraquinone derivatives as potential modulators for human aurora kinase B.

    abstract::The quest for effective anticancer therapeutics continues to be extensively pursued. Over the past century, several drugs have been developed, however, a majority of these drugs have a poor therapeutic index and increased toxicity profile. Hence, there still exists ample opportunity to discover safe and effective anti...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2020.12.024

    authors: Singh M,Malhotra L,Haque MA,Kumar M,Tikhomirov A,Litvinova V,Korolev AM,Ethayathulla AS,Das U,Shchekotikhin AE,Kaur P

    更新日期:2021-01-06 00:00:00

  • When secondary comes first--the importance of non-canonical DNA structures.

    abstract::Secondary structure-forming DNA motifs have achieved infamy because of their association with a variety of human diseases and cancer. The 3rd FASEB summer conference on dynamic DNA structures focused on the mechanisms responsible for the instabilities inherent to repetitive DNA and presented many exciting and novel as...

    journal_title:Biochimie

    pub_type:

    doi:10.1016/j.biochi.2012.10.005

    authors: Saini N,Zhang Y,Usdin K,Lobachev KS

    更新日期:2013-02-01 00:00:00

  • The role of ERG20 gene (encoding yeast farnesyl diphosphate synthase) mutation in long dolichol formation. Molecular modeling of FPP synthase.

    abstract::The yeast Saccharomyces cerevisiae strain LB332 bearing a mutation in the ERG20 gene encoding farnesyl diphosphate synthase (FPPS) synthesizes significantly longer dolichols than the wild type strain FL100 (14-31 and 14-19 isoprene units, respectively). The measurement of the short chain prenyl alcohols excreted into ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(00)01155-x

    authors: Plochocka D,Karst F,Swiezewska E,Szkopińska A

    更新日期:2000-08-01 00:00:00

  • Conformational features of bradykinin. A circular dichroism study of some peptide fragments and structural analogues of bradykinin.

    abstract::The vasoactive hormone bradykinin, its N-and C-terminal fragments and some structural analogues were studied by Circular Dichroism. Conformational features of the peptide can be detected by comparative analysis of the various CD spectra recorded as a function of aqueous pH, solvent and temperature. It is shown that th...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(79)80316-8

    authors: Lintner K,Fermandjian S,Regoli D

    更新日期:1979-01-01 00:00:00

  • LasX, a transcriptional regulator of the lactocin S biosynthetic genes in Lactobacillus sakei L45, acts both as an activator and a repressor.

    abstract::The 11 kb las locus, present on the 50 kb plasmid pCIM1, specifies the production of the lantibiotic lactocin S in Lactobacillus sakei L45. The gene cluster is organized into two oppositely orientated operons, lasAMNTUVPJW (lasA-W) and lasXY, the former of which contains the biosynthetic, immunity and transport genes....

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/s0300-9084(02)01420-7

    authors: Rawlinson EL,Nes IF,Skaugen M

    更新日期:2002-05-01 00:00:00

  • Lignan models as inhibitors of Phanerochaete chrysosporium lignin peroxidase.

    abstract::The lignan 8,8'-bis-(methylenedioxy)cinnamic acid (BMDCA) is a powerful competitive inhibitor (K1 = 2.0 microM) of the lignin peroxidase (LiP) from Phanerochaete chrysosporium and of the extracellular peroxidase of Phlebia radiata (I0.5 = 10 microM). BMDCA derivatives with the same double bond system also inhibited th...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(96)88186-7

    authors: Frías I,Trujillo JM,Romero J,Hernandez J,Pérez JA

    更新日期:1995-01-01 00:00:00

  • Comparison of physico-chemical characteristics of four laccases from different basidiomycetes.

    abstract::New strains of basidiomycetes producing extracellular laccases (Trametes ochracea 92-78, and Trametes hirsuta 56) have been found by screening of isolates of Trametes fungi. The laccases from T. hirsuta 56 and T. ochracea 92-78 as well as two laccases from previously found and described strains of basidiomycetes, name...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2004.08.005

    authors: Shleev SV,Morozova OV,Nikitina OV,Gorshina ES,Rusinova TV,Serezhenkov VA,Burbaev DS,Gazaryan IG,Yaropolov AI

    更新日期:2004-09-01 00:00:00

  • Metabolic memory and diabetic nephropathy: Beneficial effects of natural epigenetic modifiers.

    abstract::Nephropathy is one of the most frequent complications of chronic diabetes. The main reason for nephropathy despite being hyperglycemia, but it progresses even after good glycemic control has been achieved in diabetic patients. The effects of prior exposure to high blood glucose conditions depend upon the severity and ...

    journal_title:Biochimie

    pub_type: 杂志文章,评审

    doi:10.1016/j.biochi.2020.01.007

    authors: Kushwaha K,Sharma S,Gupta J

    更新日期:2020-03-01 00:00:00

  • Determination of physicochemical parameters of liposomes from concentration data.

    abstract::Determination of the physicochemical parameters of unilamellar and multilamellar liposomes from concentration data in light of a recent quantitative biodynamic concept which strictly adheres to the CGS unit system (cm, g, s) and the mass/volume chemical unit, mol.cm-3. ...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/0300-9084(87)90093-9

    authors: Hauton JC,Martigne M,Domingo N,Lafont H

    更新日期:1987-05-01 00:00:00

  • The triazatruxene derivative azatrux binds to the parallel form of the human telomeric G-quadruplex under molecular crowding conditions: biophysical and molecular modeling studies.

    abstract::The present study has employed a combination of spectroscopic, calorimetric and computational methods to explore the binding of the three side-chained triazatruxene derivative, termed azatrux, to a human telomeric G-quadruplex sequence, under conditions of molecular crowding. The binding of azatrux to the tetramolecul...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2011.05.017

    authors: Petraccone L,Fotticchia I,Cummaro A,Pagano B,Ginnari-Satriani L,Haider S,Randazzo A,Novellino E,Neidle S,Giancola C

    更新日期:2011-08-01 00:00:00

  • Attenuated mitochondrial NADP+-dependent isocitrate dehydrogenase activity induces apoptosis and hypertrophy of H9c2 cardiomyocytes.

    abstract::Oxidative stress, characterized by the accumulation of reactive oxygen species (ROS), is known to have numerous detrimental effects on the myocardium such as the induction of apoptotic cell death, hypertrophy, fibrosis, dysfunction, and dilatation. Over the past several years, we have shown that mitochondrial NADP(+)-...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/j.biochi.2013.11.016

    authors: Lee JH,Park JW

    更新日期:2014-04-01 00:00:00

  • Study of the behavior of biological molecules in a thin gap by refractive index and force measurements with an automatic surface force apparatus.

    abstract::The automation of the mica surface displacement of a surface force apparatus (SFA) increases the accuracy of the intermolecular force measurements that is obtained without the presence of the experimenter. The automatic device cancels any thermal drift and the stability of the mica surfaces is optimum. The distance be...

    journal_title:Biochimie

    pub_type: 杂志文章

    doi:10.1016/s0300-9084(00)80007-3

    authors: Gauthier-Manuel B,Gallinet JP

    更新日期:1998-05-01 00:00:00