Abstract：
:Long-term exposure to high doses of caffeine affects several aspects of nitrogen metabolism, such as purine, pyrimidine and urea synthesis. However, little is known about the mechanisms of these changes and if they occur at shorter term. We have studied in isolated hepatocytes: 1) the in vitro effect of high doses of caffeine on amino acid levels, 2) the main destination of ammonia and carbon chains from amino acid catabolism, and 3) the cytosolic and mitochondrial redox states. We have found that, whereas it has a small effect on urea synthesis and on the levels of the cofactors and intermediates, it decreases the levels of several amino acids, the gluconeogenesis and the redox state. Our results suggest that a longer exposure to caffeine is necessary to affect the normal functions of some metabolic pathways.
journal_name
Biochimiejournal_title
Biochimieauthors
Jordá A,Saéz GT,Portolés M,Pallardó FV,Jimenez-Nacher I,Gascoó Edoi
10.1016/0300-9084(88)90014-4subject
Has Abstractpub_date
1988-10-01 00:00:00pages
1417-21issue
10eissn
0300-9084issn
1638-6183pii
0300-9084(88)90014-4journal_volume
70pub_type
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