Abstract：
:Hyper- (450 mOsm/l) and hypoosmotic exposure (150 mOsm/l) of Caco-2 cells, a human intestinal epithelial cell line, induced a twofold- and a fivefold increase in the production of IL-8, a constitutively expressed cytokine, respectively. This was observed both in the presence or in the absence of added proinflammatory cytokines and the stimulatory effect of osmotic stress was additive to that induced by the cytokines. Thus, IL-8 production appeared minimal around isoosmolarity, i.e. 300 mOsm/l. Concerning the signalling pathway involved, specific inhibition of p38- or p42/44 MAP kinases decreased the IL-8 production by about 30% independently of the osmotic condition used. Inhibition of c-jun-NH2-terminal kinase (JNK) by using both dicoumarol and SP600125 totally inhibited the stimulatory effect of hypoosmolarity. Moreover, hypoosmolarity induced an about threefold increase in JNK activity demonstrating that JNK was specifically involved in the effect of hypoosmolarity on IL-8 production. This is not the case for hyperosmolarity. Such an effect of osmotic stress was not restricted to IL-8, but was also observed on the production of IL-6, a non-constitutively expressed cytokine. Again, IL-6 production appeared minimal in isoosmotic condition. Taken together, these results demonstrate that osmotic stress is a proinflammatory signal in Caco-2 cells and suggest that an osmosensor might specifically exist in intestinal epithelial cells.
journal_name
Biochimiejournal_title
Biochimieauthors
Hubert A,Cauliez B,Chedeville A,Husson A,Lavoinne Adoi
10.1016/j.biochi.2004.07.009keywords:
subject
Has Abstractpub_date
2004-08-01 00:00:00pages
533-41issue
8eissn
0300-9084issn
1638-6183pii
S0300-9084(04)00116-6journal_volume
86pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:It is now over 10 years since the discovery of peroxisome proliferator activated receptor gamma (PPAR gamma) and its unique role in adipogenesis. The subsequent identification of PPAR gamma as the target of insulin sensitizing drugs certified this ligand-regulated transcription factor as an exciting link between adipo...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2004.10.021
更新日期：2005-01-01 00:00:00
abstract：:Kissing loop interactions (KLIs) are a common motif that is critical in retroviral dimerization, viroid replication, mRNA, and riboswitches. In addition, KLIs are currently used in a variety of biotechnology applications, such as in aptamer sensors, RNA scaffolds and to stabilize vaccines for therapeutics. Here we des...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.11.012
更新日期：2019-02-01 00:00:00
abstract：:In search for yet uncharacterized proteins involved in lipid metabolism of the chicken, we have isolated a hitherto unknown protein from the serum lipoprotein fraction with a buoyant density of ≤1.063 g/ml. Data obtained by protein microsequencing and molecular cloning of cDNA defined a 537 bp cDNA encoding a precurso...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.09.005
更新日期：2013-12-01 00:00:00
abstract：:Our understanding of plant meiosis is rapidly increasing thanks to the model Arabidopsis thaliana. Here we present the results of a screening for meiotic mutants carried out with a library containing 30,719 T-DNA insertion lines. An average of one mutant per 1000 lines was recovered. Several phenotypic classes could b...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(01)01348-7
更新日期：2001-11-01 00:00:00
abstract：:The multisubunit Mediator is an evolutionary conserved transcriptional coregulatory complex in eukaryotes. It is needed for the transcriptional regulation of gene expression in general as well as in a gene specific manner. Mediator complex subunits interact with different transcription factors as well as components of...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2017.10.012
更新日期：2018-01-01 00:00:00
abstract：:Multiple forms of acetylcholinesterase have been studied in Goldfish adapted to 15 degrees C and 25 degrees C. Three forms of enzyme (sedimenting at 18S, 12S and 5S) could be distinguished by sucrose gradient centrifugation. Their molecular and enzymatic properties did not vary with environmental temperature. It was n...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80257-x
更新日期：1976-01-01 00:00:00
abstract：:The exosome is a complex of eleven subunits in yeast, involved in RNA processing and degradation. Despite the extensive in vivo functional studies of the exosome, little information is yet available on the structure of the complex and on the RNase and RNA binding activities of the individual subunits. The current mode...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2007.01.011
更新日期：2007-05-01 00:00:00
abstract：:A major tolerance mechanism that functions to replicate damaged genomic DNA across lesions that have escaped elimination by repair mechanism is translesion DNA synthesis (TLS). DNA polymerase kappa (Pol kappa), a specialised low-fidelity DNA polymerase which is able to perform DNA synthesis across several damaged base...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2005.02.007
更新日期：2005-07-01 00:00:00
abstract：:Long non-coding RNAs constitute the most abundant part of the transcribed mammalian genome. lncRNAs affect all essential processes in the living cell including transcription, splicing, translation, replication, shaping of chromatin and post translational modification of proteins. Alterations in lncRNA expression have ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2016.06.007
更新日期：2016-12-01 00:00:00
abstract：:Thyroid peroxidase is a member of a family of mammalian peroxidases that includes myeloperoxidase, lactoperoxidase, eosinophil peroxidase, and salivary peroxidase. Protein sequences showing a high degree of sequence similarity with mammalian peroxidases have recently been observed in several invertebrate species. A mu...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(99)80110-2
更新日期：1999-05-01 00:00:00
abstract：:The nucleotide sequence of the gene encoding chicken ribosomal protein L7a was determined. The gene contains eight exons and seven introns, which spread over 3613 nucleotides. The transcription initiation sites were located on four consecutive nucleotides GCCC, which are at the 5' terminus of a short polypyrimidine tr...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(93)90128-f
更新日期：1993-01-01 00:00:00
abstract：:From investigation of 2000 soil isolates, we identified two serine-type amidohydrolases that can hydrolyze d-aminoacyl derivatives from the culture supernatant of Streptomyces species 82F2 and 83D12. The enzymes, redesignated as 82F2-DAP and 83D12-DAP, were purified for homogeneity and characterized. Each enzyme had m...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.04.020
更新日期：2011-09-01 00:00:00
abstract：:The analysis of RNA from L5178 Y cells, by electrophoreis in 1.7% polyacrylamide gel, after short time labelling with [methyl-3H] methionine, shows that the three molecular species 47S, 46S and 45S RNA from the ribosomal RNA "45S fraction" are methylated. Comparative studies of the kinetics obtained after labelling fo...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80544-5
更新日期：1975-11-01 00:00:00
abstract：:A HU-like protein (HBl) of Bifidobacterium longum was purified and characterized. HBl is heat-stable and acid-resistant, and has a molecular weight of about 9.1 kDa as estimated by its mobility on electrophoresis. HBl is intermediate in basicity (pI 9.8) between the HU-1 and HU-2 proteins of Escherichia coli, and is d...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(90)90074-q
更新日期：1990-04-01 00:00:00
abstract：:Three trends are seen in the organization of ribosomal DNA genes during evolution: 1) gradual separation and separability of the regulation of transcription of 5S and larger subunit rRNAs; 2) retention of a transcription unit containing both large and small rRNAs; and 3) clustering of genes for both 5S and 18S-28S rDN...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(91)90042-y
更新日期：1991-06-01 00:00:00
abstract：:Murine L5178Y cell ribosomes were dissociated into subunits either with potassium chloride in the presence of puromycin or with the chelating agent EDTA. The proteins of ribosomal subunits obtained by these different methods were compared by means of bidimensional polyacrylamide gel electrophoresis. KCl-derived 60S an...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(80)80225-2
更新日期：1979-01-01 00:00:00
abstract：:In recent years the chaperone HSC-70 has become a target for drug design with a strong focus in anticancer therapies. In our study of possible inhibitors of HSC-70 enzymatic activity we screened compounds by NMR as well as X-ray crystallography. As part of our screening efforts we crystallized the human HSC-70 ATP bin...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.11.012
更新日期：2015-01-01 00:00:00
abstract：:The study of modified nucleoside contributions to RNA chemistry, structure and function has been thwarted by the lack of a site-selected method of incorporation which is both versatile and adaptable to present synthetic technologies. A reproducible and versatile site-selected incorporation of nine differently modified...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(96)88115-6
更新日期：1995-01-01 00:00:00
abstract：:Phospholipid analysis of the membranes associated with fast sedimenting folded chromosomes prepared by lysis of E. coli CR 34 shows that both inner and outer membranes are parts of the complex, in proportions not very different from that found in the whole bacteria. During the preparation of the folded chromosomes, th...
journal_title：Biochimie
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:Cell cycle-related and expression elevated protein in tumor (CREPT) is emerging as a novel cancer-related gene that is dysregulated in many kinds of malignancies. However, the expression and biological role of CREPT in glioma remains unclear. In the present study, we aimed to explore the potential function and regulat...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.04.014
更新日期：2019-07-01 00:00:00
abstract：:The redox homeostasis of cytoplasm is maintained by a series of disulfide exchange reactions mediated by proteins belonging to the thioredoxin superfamily. Thioredoxin and thioredoxin reductase, being the major members of the family, play a key role in oxidative stress response of Staphylococcus aureus. In this report...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.01.019
更新日期：2019-05-01 00:00:00
abstract：:Peroxisomal ABC transporters encoded by the ABCD genes are thought to participate in the import of specific fatty acids in the peroxisomal matrix. ABCD1 deficiency is associated with X-linked adrenoleukodystrophy (X-ALD), the most frequent peroxisomal disorder which is characterized by the accumulation of saturated ve...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2008.05.022
更新日期：2008-10-01 00:00:00
abstract：:GABARAPL1 belongs to the small family of GABARAP proteins (including GABARAP, GABARAPL1 and GABARAPL2/GATE-16), one of the two subfamilies of the yeast Atg8 orthologue. GABARAPL1 is involved in the intracellular transport of receptors, via an interaction with tubulin and GABA(A) or kappa opioid receptors, and also par...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.11.006
更新日期：2012-03-01 00:00:00
abstract：:The Nicotiana tabacum Nt-4/1 protein is a plant-specific protein of unknown function. Analysis of bacterially expressed Nt-4/1 protein in vitro revealed that the protein secondary structure is mostly alpha-helical and suggested that it could consist of three structural domains. Earlier studies of At-4/1, the Arabidops...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.02.015
更新日期：2013-07-01 00:00:00
abstract：:Hydroxylamine and its derivatives of general formula H2NOR react with aldehydes and aldimines to produce oximes. If R corresponds to the side chain of a natural amino acid, such compounds can be thought of as analogs of the corresponding amino acids, lacking the alpha-carboxylate group. Oximes formed between such comp...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(89)90175-2
更新日期：1989-04-01 00:00:00
abstract：:Evidence from clinical, animal and cell culture studies demonstrates that increased autotaxin (ATX) expression is responsible for enhancing tumor progression, cell migration, metastases, angiogenesis and chemo-resistance. These effects depend mainly on the rapid formation of lysophosphatidate (LPA) by ATX. Circulating...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2010.08.002
更新日期：2011-01-01 00:00:00
abstract：:Pleurocidin (GWGSFFKKAAHVGKHVGKAALTHYL-NH(2)), found in skin mucous secretions of the winter flounder Pleuronectes americanus, is known to possess a high potency and broad-spectrum antimicrobial peptide without cytotoxicity. In this study, to investigate the impact of pleurocidin on apoptotic progress, we observed mor...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.07.011
更新日期：2011-10-01 00:00:00
abstract：UNLABELLED:Pancreatic cancer (PC) is one of the most lethal human malignancies and a major health problem. Patients diagnosed with PC and treated with conventional approaches have an overall 5-year survival rate of less than 5%. Novel strategies are needed to treat this disease. Herein, we propose a combinatorial strat...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2015.07.012
更新日期：2015-09-01 00:00:00
abstract：:Cysteine-rich secretory proteins (CRISPs), characterized by 16 conserved cysteines, are distributed in a wide range of organisms, such as secernenteas, amphibians, reptiles and mammals. In the previous studies, a novel CRISP family member (cysteine-rich buccal gland protein, CRBGP) was separated from the buccal gland ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2015.11.020
更新日期：2016-04-01 00:00:00
abstract：:A reliable phylogenetic reconstruction of the evolutionary history of contemporary species depends on a robust identification of the universal common ancestor (UCA) at the root of the Tree of Life (ToL). That root polarizes the tree so that the evolutionary succession of ancestors to descendants is discernable. In eff...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.04.014
更新日期：2017-07-01 00:00:00
