Abstract:
:Kinetic properties of PGM1 and PGM2 phosphoglucomutase "primary" isoenzymes from human erythrocytes were studied. The two enzyme forms share a "ping-pong" kinetic mechanism and show similar Km for substrate (glucose 1-P) and cofactor (glucose 1,6-P2). Micromolar concentrations of fructose 1,6-P2 and glycerate 2,3-P2 inhibit both PGM1 and PGM2 isoenzymes to a similar extent. The sole PGM2 form is affected by ribose monophosphates (ribose 1-P and ribose 5-P) that act as mutase inhibitors vs. glucose 1,6-P2 and as apparent activators vs. glucose 1-P. The interaction between PGM2 isoenzyme and ribose monophosphates is discussed in the light of the ability of this form to also display phosphoribomutase activity.
journal_name
Biochimiejournal_title
Biochimieauthors
Ninfali P,Accorsi A,Palma F,Fazi A,Piatti E,Chiarantini L,Fornaini Gdoi
10.1016/0300-9084(84)90115-9subject
Has Abstractpub_date
1984-09-01 00:00:00pages
617-23issue
9-10eissn
0300-9084issn
1638-6183pii
0300-9084(84)90115-9journal_volume
66pub_type
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