Abstract：
:Tetramolecular G-quadruplexes result from the association of four guanine-rich strands. Modification of the backbone strand or the guanine bases of the oligonucleotide may improve stability or introduce new functionalities. In this regard, the 8 position of a guanosine is particularly suitable for introduction of modifications since as it is positioned in the groove of the quadruplex structure. Modifications at this position should not interfere with structural assembly as would changes at Watson-Crick and Hoogsteen sites. In this study, we investigated the effect of an 8-methyl-2'-deoxyguanosine residue (M) on the structure and stability of tetramolecular parallel G-quadruplexes. In some cases, the presence of this residue resulted in the formation of unusual quadruplex structures containing all-syn tetrads. Furthermore, the modified nucleoside M at the 5'-end of the sequence accelerated quadruplex formation by 15-fold or more relative to the unmodified oligonucleotide, which makes this nucleobase an attractive replacement for guanine in the context of tetramolecular parallel quadruplexes.
journal_name
Biochimiejournal_title
Biochimieauthors
Tran PL,Virgilio A,Esposito V,Citarella G,Mergny JL,Galeone Adoi
10.1016/j.biochi.2010.10.011subject
Has Abstractpub_date
2011-03-01 00:00:00pages
399-408issue
3eissn
0300-9084issn
1638-6183pii
S0300-9084(10)00371-8journal_volume
93pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:The effect of alpha-tocopherol on the structure and thermotropic phase behaviour of 1-palmitoyl-2-oleoyl-phosphatidylethanolamine dispersed in excess water was examined by synchrotron X-ray diffraction and differential scanning calorimetry. Small- and wide-angle X-ray scattering intensity profiles were recorded from m...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2006.07.017
更新日期：2006-12-01 00:00:00
abstract：:We present here, a new method for coupling sulfhydryl groups (SH) to low-density lipoprotein (LDL) surface. This method uses homocysteine thiolactone (HCTL) which reacts with lysine residues in a very mild manner, and permits the selection of the number of SH bound per LDL. Under our experimental conditions (8 SH/LDL)...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(86)80166-3
更新日期：1986-05-01 00:00:00
abstract：:Trypsin and pronase treatment of purified human neutral bronchial mucins released small fragments from the C-terminal end of these molecules and resulted in slight increases in their sedimentation coefficient presumably reflecting conformational changes. The antigenic determinant of neutral bronchial mucins which appe...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80444-0
更新日期：1976-01-01 00:00:00
abstract：:In 1956, when we started our collaboration, both Alick Isaacs and myself had done previous work on interference between inactive and active influenza viruses. We were aware of the state interference research had reached and of the two alternative explanations that had been envisaged. ...
journal_title：Biochimie
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.biochi.2007.04.007
更新日期：2007-06-01 00:00:00
abstract：:The systems involving folate and cobalamin have several features in common: 1) their dietary forms require luminal digestion for absorption; 2) intestinal bacteria in the upper intestine synthesize and utilize both vitamins, creating possible competition for the nutrients; 3) there is one major intestinal brush border...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.11.006
更新日期：2016-07-01 00:00:00
abstract：:1) Isonitrile derivatives of synthetic polyamides, polyesters and polyalcohols, of polysaccharides such as cellulose, cross-linked dextran and agarose, linear dextran and of cross-linked and linear polyacrylamide were prepared. 2) Proteins and low-molecular-weight ligands were bound covalently to these materials by fo...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(80)80172-6
更新日期：1980-01-01 00:00:00
abstract：:In the bacterium Escherichia coli, H-NS-(H1, H1a) is a heat-stable protein with a molecular mass of 15.5 kDa involved in nucleoid organisation and gene regulation linked to certain signal transduction pathways. We have shown that, following addition of preparations of everted inner membrane vesicles, heat-stable cleav...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(97)80025-9
更新日期：1997-06-01 00:00:00
abstract：:Five 4-arylcoumarins (1c-g) and twelve 3,4-dihydro-4-arylcoumarins (2a-l) were synthesized and tested for antioxidant activity, antitumor activity, toxicity and structure-activity relationships analysis. 4-Arylcoumarins and 3,4-dihydro-4-arylcoumarins that possess two hydroxyl groups in ortho position, such as 1d, 1f,...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.03.014
更新日期：2014-12-01 00:00:00
abstract：:Theoretic and experimental arguments are surveyed which justify the setting up, within the family of pyridoxal-P-dependent lyases, of a special subgroup that comprizes several enzymes catalyzing exclusively beta-replacement reactions of alpha-aminoacids with electronegative substituents in the beta-position. The autho...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(76)80351-3
更新日期：1976-01-01 00:00:00
abstract：:Phospholipid metabolism of 3T3 mouse fibroblasts has been studied after serum stimulation of arrested cells. The study of [32Pi] incorporation shows : a) in the case of PE and PC an early peak of incorporation in the G1 phase of the cell cycle, 6 hours after serum addition; b) in the case of PI an intense initial incr...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(79)80448-4
更新日期：1978-01-01 00:00:00
abstract：:Binding of indoloquinolines with different aliphatic side chains to a parallel G-quadruplex derived from the MYC promoter sequence was characterized by optical and calorimetric measurements. ITC experiments performed at different temperatures enabled the determination of molar heat capacity changes upon quadruplex bin...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.11.015
更新日期：2019-02-01 00:00:00
abstract：:Glycogen synthase I was purified from bovine polymorphonuclear leucocytes (PMNs) by a procedure involving concanavalin A-Sepharose affinity chromatography. The purified glycogen-bound glycogen synthase I had a specific activity of 9.83 U/mg protein and the glycogen free enzyme 21 U/mg protein. Molecular ratio of the n...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80201-7
更新日期：1985-06-01 00:00:00
abstract：:DNA replication in Escherichia coli is controlled at the initiation stage, possibly by regulation of the essential activity of DnaA protein. The cellular membrane has long been hypothesized to be involved in chromosomal replication. Accumulating evidence, both in vitro and in vivo, that supports the importance of memb...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(00)01224-4
更新日期：2001-01-01 00:00:00
abstract：:Nitrogen fixation in A. vinelandii and R. leguminosarum bacteroides shows identical characteristics with respect to the dependence on membrane energization, the sensitivity to uncouplers, the ATP/ADP-ratio, and the dependences on flavodoxinhydroquinone as electrondonor. Although we have been successful in preparing in...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(78)80820-7
更新日期：1978-01-01 00:00:00
abstract：:Translational autocontrol of the expression of the ribosomal protein S15 proceeds through the transitory formation of a pseudoknot. A synopsis of the known data is used to propose a molecular model of the mechanism involved and for the role of the pseudoknot. This latter structure is able to recruit 30S ribosomal subu...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(96)80003-4
更新日期：1996-01-01 00:00:00
abstract：:Adrenoleukodystrophy (ALD) is an X-linked peroxisomal disorder characterized by a progressive demyelination of the central nervous system and adrenal insufficiency. Clinical phenotypes of different severity are frequently observed within the same kindred. ALD is characterized biochemically by the accumulation of very-...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(93)90089-b
更新日期：1993-01-01 00:00:00
abstract：:The action of indomethacin on the lactogenic activity of prolactin has been evaluated usind the technique of rabbit mammary gland organ culture. Indomethacin is totally unable to inhibit prolactin action as estimated by lactose synthetase activity and casein synthesis. These data suggest, as opposed to previous works...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(80)80060-5
更新日期：1980-01-01 00:00:00
abstract：:Phospholipid analysis of the membranes associated with fast sedimenting folded chromosomes prepared by lysis of E. coli CR 34 shows that both inner and outer membranes are parts of the complex, in proportions not very different from that found in the whole bacteria. During the preparation of the folded chromosomes, th...
journal_title：Biochimie
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:Hyper- (450 mOsm/l) and hypoosmotic exposure (150 mOsm/l) of Caco-2 cells, a human intestinal epithelial cell line, induced a twofold- and a fivefold increase in the production of IL-8, a constitutively expressed cytokine, respectively. This was observed both in the presence or in the absence of added proinflammatory ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2004.07.009
更新日期：2004-08-01 00:00:00
abstract：:Epithelial Ca(2+)-regulation, which governs cornified envelope formation in the skin epidermis and hair follicles, closely coincides with the expression of S100A3, filaggrin and trichohyalin, and the post-translational modification of these proteins by Ca(2+)-dependent peptidylarginine deiminases. This review summariz...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2011.05.028
更新日期：2011-12-01 00:00:00
abstract：:The function of glycerophosphate and malate-aspartate shuttles during glucose metabolism in two strains of Ehrlich ascites tumor cells was evaluated by several experimental approaches. The activities of the enzymes involved in these shuttle systems were assayed in the cytosolic and mitochondrial compartments after cel...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80055-9
更新日期：1985-02-01 00:00:00
abstract：:Apolipoprotein E (apoE) has been genetically linked to late-onset Alzheimer's disease. From the three common alleles (epsilon2, epsilon3 and epsilon4), epsilon4 has been suggested to promote amyloid beta (Ass) plaque fibrillation, one hallmark of Alzheimer's disease. It has been demonstrated that altered lipid content...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.10.007
更新日期：2006-05-01 00:00:00
abstract：:The functions of residues 57-RY-58, G60, L77, 80-GSGR-83, I101, T104, 134-GY-135, N155, V157 and 160-LV-161 in human arsenic (III) methyltransferase (hAS3MT) 5 Å around S-adenosylmethionine (SAM) have not been studied. Herein, sixteen mutants were designed by substituting these residues with Ala. Mutants G60A, G80A, I...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.10.014
更新日期：2014-12-01 00:00:00
abstract：:At least 6 N-acetylglucosaminyltransferases (GlcNAc-T I, II, III, IV, V and VI) are involved in initiating the synthesis of the various branches found in complex asparagine-linked oligosaccharides (N-glycans), as indicated below: GlcNAc beta 1-6 GlcNAc-T V GlcNAc beta 1-4 GlcNAc-T VI GlcNAc beta 1-2Man alpha 1-6 GlcNA...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(88)90289-1
更新日期：1988-11-01 00:00:00
abstract：:We have cloned a homologue of the translationally controlled tumor protein (TCTP) from the buccal gland of Lampetra japonica according to information from a cDNA library and primary analysis of expressed sequence tags. Sequence analysis of L. japonica TCTP showed that it had two signature regions of high sequence homo...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2008.08.002
更新日期：2008-11-01 00:00:00
abstract：:Bacterial survive and respond to adverse changes in the environment by regulating gene transcription through two-component regulatory systems. In Salmonella Typhimurium the STM1485 gene expression is induced under low pH (4.5) during replication inside the epithelial host cell, but it is not involved in sensing or res...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.02.005
更新日期：2019-05-01 00:00:00
abstract：:The study of modified nucleoside contributions to RNA chemistry, structure and function has been thwarted by the lack of a site-selected method of incorporation which is both versatile and adaptable to present synthetic technologies. A reproducible and versatile site-selected incorporation of nine differently modified...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(96)88115-6
更新日期：1995-01-01 00:00:00
abstract：:Since the initial identification of leptin as the product of the ob gene in 1994, the signaling pathways by which this hormone alters cell physiology have been the subject of extensive investigations. The fact that leptin can induce nitric oxide (NO) production was first demonstrated in studies of the pituitary gland ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2012.06.017
更新日期：2012-10-01 00:00:00
abstract：:The structure of ATP synthase subunit 4 was determined by using the oligonucleotide probe procedure. This subunit is the fourth polypeptide of the complex when classifying subunits in order of decreasing molecular mass. Its relative molecular mass is 25 kDa. The ATP4 gene was isolated and sequenced. The nucleotide seq...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(89)90073-4
更新日期：1989-08-01 00:00:00
abstract：:Snake venom phospholipases A2 (PLA2) share high sequence identities and a conserved structural scaffold, but show important functional differences. Only a few PLA2s have been purified and characterized from coral snake (Micrurus spp.) venoms, and their role in envenomation remains largely unknown. In this report, we d...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.03.008
更新日期：2017-06-01 00:00:00
