Abstract：
:The function of glycerophosphate and malate-aspartate shuttles during glucose metabolism in two strains of Ehrlich ascites tumor cells was evaluated by several experimental approaches. The activities of the enzymes involved in these shuttle systems were assayed in the cytosolic and mitochondrial compartments after cell fractionation by the digitonin method. The glycerophosphate shuttle can be ruled out because of the lack of relevant enzymatic activities, and the failure of glucose to increase rotenone-inhibited respiration. Analysis of glycolytic flux in the presence of aminooxyacetate indicates that the activity of malate-aspartate shuttle may be very low. Balance studies of glucose uptake and lactate production suggest the existence of other pathways for the reoxidation of cytosolic NADH, which are acetyl-CoA dependent. Estimation of citrate synthase and ATP citrate lyase, in addition to the observed high activity of malate dehydrogenase, suggests a malate-citrate shuttle.
journal_name
Biochimiejournal_title
Biochimieauthors
Sánchez-Jiménez F,Martínez P,Núñez de Castro I,Olavarría JSdoi
10.1016/s0300-9084(85)80055-9subject
Has Abstractpub_date
1985-02-01 00:00:00pages
259-64issue
2eissn
0300-9084issn
1638-6183pii
S0300-9084(85)80055-9journal_volume
67pub_type
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