Membrane energization in relation with nitrogen fixation in Azotobacter vinelandii and Rhizobium leguminosarum bacteroids.

abstract：:Personalized and regenerative medicine is an emerging therapeutic strategy that is based on cell biology and biomedical engineering used to develop biological substitutes to maintain normal function or restore damaged tissues and organs. The secretory capacities of different cell types are now explored as such possibl...

journal_title：Biochimie

authors： Gaceb A,Barbariga M,Özen I,Paul G

更新日期：2018-12-01 00:00:00

abstract：:A variety of species of GPDH undergo acylation at two of the four active cystein sites per mole of tetrameric enzyme. This reaction requires tightly bound NAD+, a situation restricted to two of the four NAD sites per tetramer. S leads to N acyl transfer from cysteins to lysine in the diacyl enzyme yields an inactive e...

journal_title：Biochimie

authors： Malhotra OP,Bernhard SA,Seydoux F

更新日期：1981-02-01 00:00:00

abstract：:Peptidase family S46 consists of two types of dipeptidyl-peptidases (DPPs), DPP7 and DPP11, which liberate dipeptides from the N-termini of polypeptides along with the penultimate hydrophobic and acidic residues, respectively. Their specificities are primarily defined by a single amino acid residue, Gly673 in DPP7 and...

journal_title：Biochimie

authors： Nemoto TK,Bezerra GA,Ono T,Nishimata H,Fujiwara T,Ohara-Nemoto Y

更新日期：2018-04-01 00:00:00

abstract：:Seryl-tRNA synthetases (SerRS) covalently attach serine to cognate tRNA(Ser). Atypical SerRSs, considerably different from canonical enzymes, have been found in methanogenic archaea. A crystal structure of methanogenic-type SerRS revealed a motif within the active site (serine ordering loop; SOL), which undergoes a no...

journal_title：Biochimie

authors： Dulic M,Pozar J,Bilokapic S,Weygand-Durasevic I,Gruic-Sovulj I

更新日期：2011-10-01 00:00:00

abstract：:TcrX/Y is one of the twelve two component system (TCS) present in Mycobacterium tuberculosis. We have investigated the TcrX/Y interaction by in silico studies, pull down assay, radioactive phosphotransfer, surface plasmon resonance as well as crosstalk analysis of TcrY with TcrA - a non-cognate response regulator. Seq...

journal_title：Biochimie

authors： Bhattacharya M,Biswas A,Das AK

更新日期：2010-03-01 00:00:00

abstract：:Homocysteine (HCY) is a degradation product of the methionine pathway. The B vitamins, in particular vitamin B12 and folate, are the primary nutritional determinant of HCY levels and therefore their deficiencies result in hyperhomocysteinaemia (HHCY). Prevalence of hyperhomocysteinemia (HHCY) and related dietary defic...

journal_title：Biochimie

authors： Feigerlova E,Demarquet L,Guéant JL

更新日期：2016-07-01 00:00:00

abstract：:Multiple forms of acetylcholinesterase have been studied in Goldfish adapted to 15 degrees C and 25 degrees C. Three forms of enzyme (sedimenting at 18S, 12S and 5S) could be distinguished by sucrose gradient centrifugation. Their molecular and enzymatic properties did not vary with environmental temperature. It was n...

journal_title：Biochimie

authors： Guillon G,Massoulié J,Guillon G,Massoulié J

更新日期：1976-01-01 00:00:00

abstract：:Porcine thyroid cells in primary cultures form either monolayers or, when they are stimulated by thyrotropin (TSH), follicles. This system, monolayer-follicle associated cell culture, determines two morphofunctional states of the thyroid cell in vitro. These cells divide, when grown in monolayer. In this article we de...

journal_title：Biochimie

authors： Fayet G,Hovsépian S

更新日期：1980-01-01 00:00:00

abstract：:Complexes of purified 40S ribosomal subunits and initiation factor 3 from rabbit reticulocytes were crosslinked using the reversible protein crosslinking reagent, 2-iminothiolane, under conditions shown previously to lead to the formation of dimers between 40S proteins but not higher multimers. The activity of both th...

journal_title：Biochimie

authors： Tolan DR,Hershey JW,Traut RT

更新日期：1983-07-01 00:00:00

abstract：:Human telomeres are intrinsically dynamic structures, with multiple biological processes operating to generate substantial length heterogeneity. Processes that operate specifically at the terminus, that include the end-replication problem coupled with C-strand resection, result in gradual telomere erosion with ongoing...

journal_title：Biochimie

authors： Baird DM

更新日期：2008-01-01 00:00:00

abstract：:In the process of L-phenylalanine oxidation by Proteus mirabilis cytoplasmic membrane, hydrogen peroxide was produced at a rate corresponding to 1-3 per cent of the total electron flow (30-110 nmoles min-1mg-1). Peroxide was estimated using a fluorimetric assay with horseradish peroxidase, or by anodic oxidation on a ...

journal_title：Biochimie

authors： Sauret-Ignazi G,Laboure-Rossat AM,Jouve HM,Pelmont J

更新日期：1982-10-01 00:00:00

abstract：:Leishmania parasites cause two million new cases of leishmaniasis each year with several hundreds of millions of people at risk. Due to the paucity and shortcomings of available drugs, we have undertaken the crystal structure determination of a key enzyme from Leishmania major in hopes of creating a platform for the r...

journal_title：Biochimie

authors： Larson ET,Kim JE,Zucker FH,Kelley A,Mueller N,Napuli AJ,Verlinde CL,Fan E,Buckner FS,Van Voorhis WC,Merritt EA,Hol WG

更新日期：2011-03-01 00:00:00

abstract：:Although halogenated hydrocarbons are noted for low chemical reactivity, small amounts are toxic to humans. Cytochromes P450 have been implicated in transforming these compounds to more reactive species, under anaerobic conditions, through reduction at the heme. A significant amount of effort has been directed toward ...

journal_title：Biochimie

authors： Manchester JI,Ornstein RL

更新日期：1996-01-01 00:00:00

abstract：:The present study has employed a combination of spectroscopic, calorimetric and computational methods to explore the binding of the three side-chained triazatruxene derivative, termed azatrux, to a human telomeric G-quadruplex sequence, under conditions of molecular crowding. The binding of azatrux to the tetramolecul...

journal_title：Biochimie

authors： Petraccone L,Fotticchia I,Cummaro A,Pagano B,Ginnari-Satriani L,Haider S,Randazzo A,Novellino E,Neidle S,Giancola C

更新日期：2011-08-01 00:00:00

abstract：:Administration of beta-adrenergic agonists to domestic species can lead to skeletal muscle hypertrophy, probably by reducing the rate of myofibrillar protein breakdown. Myofibrillar breakdown is associated with the calcium-dependent proteinase system (calpains I,II and calpastatin) whose activity also changes during b...

journal_title：Biochimie

authors： Bardsley RG,Allcock SM,Dawson JM,Dumelow NW,Higgins JA,Lasslett YV,Lockley AK,Parr T,Buttery PJ

更新日期：1992-03-01 00:00:00

abstract：:In all living cells 3' ends of RNA are posttranscriptionally elongated or shortened by nucleotidyl transferases and ribonucleases. The detailed analysis of the rpsO mRNA of Escherichia coli presented here demonstrates that transcription terminates in vivo at two sites located seven and eight nucleotides downstream fro...

journal_title：Biochimie

authors： Folichon M,Marujo PE,Arluison V,Le Derout J,Pellegrini O,Hajnsdorf E,Régnier P

更新日期：2005-09-01 00:00:00

abstract：:The eukaryotic genome is compacted in the cell nucleus, in a way that allows its faithful and ordered replication each cell cycle. Chromatin is organized into topologically constrained loops that are anchored to the nuclear matrix by specific attachment regions (SARs). Chromatin loops were proposed to correspond to re...

journal_title：Biochimie

authors： Hyrien O,Maric C,Lucas I

更新日期：1997-10-01 00:00:00

abstract：:At the first symptoms of organisation of the silkgland in the embryo, mitoses stop and nuclei start to grow. Through autoradiographic studies, performed after sequenced labeling with [3H] and [14C] thymidine, the durations of the different phases of DNA synthesis cycles (T = 42 to 48 h, S = 22 to 25 h, G = 20 to 23 h)...

journal_title：Biochimie

authors： Perdrix-Gillot S

更新日期：1979-01-01 00:00:00

abstract：:Maximum 125I-IGF-I/Sm-C total binding to chick embryo fibroblasts was 3% at +37 degrees C and decreased to less than 1% in presence of 2.8 X 10(-9) M unlabelled IGF-I/Sm-C. Insulin did not compete with IGF-I/Sm-C for the binding to cells. Biological action of IGF-I/Sm-C was evaluated on 2-deoxyglucose and alpha-aminoi...

journal_title：Biochimie

authors： Cynober L,Aussel C,Chatelain P,Vaubourdolle M,Agneray J,Ekindjian OG

更新日期：1985-10-01 00:00:00

abstract：:The gene for the ribosomal protein L5 from the archaebacterium Sulfolobus acidocaldarius has been isolated and sequenced. The gene codes for a basic protein of molecular weight 29 165 Da. This protein shows substantial similarity to the equivalent protein from other archaebacteria as well as from yeast, and considerab...

journal_title：Biochimie

authors： Yang D,Günther I,Matheson AT,Auer J,Spicker G,Böck A

更新日期：1991-06-01 00:00:00

abstract：:The protein "BCL-2-associated athanogene-1" (BAG-1), which exists in multiple isoforms, promotes cancer cell survival and is overexpressed in many different cancers. As a result, BAG-1-targeted therapy appears to be a promising strategy with which to treat cancer. It has previously been shown that the 5'UTR of the BAG...

journal_title：Biochimie

authors： Bolduc F,Turcotte MA,Perreault JP

更新日期：2020-09-01 00:00:00

abstract：:Lipolytic activities of Yarrowia lipolytica LIP2 lipase (YLLIP2), human pancreatic (HPL) and dog gastric (DGL) lipases were first compared using lecithin-stabilized triacylglycerol (TAG) emulsions (Intralipid) at various pH and bile salt concentrations. Like DGL, YLLIP2 was able to hydrolyze TAG droplets covered by a ...

journal_title：Biochimie

authors： Bénarouche A,Point V,Carrière F,Cavalier JF

更新日期：2014-07-01 00:00:00

abstract：:An apparatus for chemi- and bioluminescence measurements is described. The main features are in high sensitivity: for example, 5 10(-12) M adenosine triphosphate, 5 10(-7) M glucose, 2 10(-9) M perhydrol and 4 10(-13) M peroxidase are easily detected; its fast mixing time and the possibility of injecting a reactant, a...

journal_title：Biochimie

authors： Arrio B,Lecuyer B,Dupaix A,Volfin P,Jousset M,Carrette A

更新日期：1980-01-01 00:00:00

abstract：:Pentatricopeptide repeat (PPR) proteins are a large family of modular RNA-binding proteins which mediate several aspects of gene expression primarily in organelles but also in the nucleus. These proteins facilitate processing, splicing, editing, stability and translation of RNAs. While major advances in PPR research h...

journal_title：Biochimie

authors： Manna S

更新日期：2015-06-01 00:00:00

abstract：:The family of interferon regulatory transcription factors (IRF) participates in the virus-induced and dsRNA-stimulated transcriptional regulation of either type I IFN genes or a definite set of genes which can also be activated by IFN. In this review, we place emphasis on the role of IRF-3 that associates with the coa...

journal_title：Biochimie

authors： Bragança J,Civas A

更新日期：1998-08-01 00:00:00

abstract：:A novel approach to genetic sequence analysis is presented. This approach, based on compression of algorithms, has been launched simultaneously by Grumbach and Tahi, Milosavljevic and Rivals. To reduce the description of an object, a compression algorithm replaces some regularities in the description by special codes....

journal_title：Biochimie

authors： Rivals E,Dauchet M,Delahaye JP,Delgrange O

更新日期：1996-01-01 00:00:00

abstract：:The Escherichia coli low molecular mass penicillin-binding proteins (PBP4, PBP5 and PBP6) are a group of penicillin-sensitive enzymes involved in the final stages of cell wall assembly. It has been suggested that these proteins may interact with the periplasmic face of the inner membrane via C-terminal amphiphilic alp...

journal_title：Biochimie

authors： Harris F,Phoenix DA

更新日期：1997-04-01 00:00:00

abstract：:The term 'RNA editing' was used for the first time in 1986 to describe the process of uridylate insertion into trypanosomal mitochondrial transcripts. Since then, the term has been used more generally to describe a large variety of processes involving base insertions, deletions and conversions that generate RNAs with ...

journal_title：Biochimie

authors： Blanc V,Jordana X,Litvak S,Araya A

更新日期：1996-01-01 00:00:00

abstract：:The variable manifestation of phenotypes that occur in patients with neurofibromatosis type 1 (NF1) includes benign and malignant neurocutaneous tumors for which no adequate treatment exists. Cell-based screening of known bioactive compounds library identified the protein phosphatase 2A (PP2A) inhibitor Cantharidin an...

journal_title：Biochimie

authors： Semenova G,Stepanova DS,Deyev SM,Chernoff J

更新日期：2017-04-01 00:00:00

abstract：:A synthetic gene of yeast aspartic acid tRNA with a promoter for phage T7 RNA polymerase was cloned in Escherichia coli. The in vitro transcribed tRNA(Asp) molecules are deprived of modified nucleotides and retain their aspartylation capacity. The solution conformation of these molecules was mapped with chemical struc...

journal_title：Biochimie

authors： Perret V,Garcia A,Puglisi J,Grosjean H,Ebel JP,Florentz C,Giegé R

更新日期：1990-10-01 00:00:00