Abstract：
:Multiple forms of acetylcholinesterase have been studied in Goldfish adapted to 15 degrees C and 25 degrees C. Three forms of enzyme (sedimenting at 18S, 12S and 5S) could be distinguished by sucrose gradient centrifugation. Their molecular and enzymatic properties did not vary with environmental temperature. It was noted that the relative proportion of the three forms differed between brain and muscle extracts, the 18S forms predominating in muscle (about 75 per cent) the 12S forms being more abundant in the brain, suggesting a physiological differentiation between these forms. The latter form varied from 48 per cent to 70 per cent in Goldfish adapted to 15 degrees and 25 degrees C respectively. The relative efficiency of saline buffer solubilization for each form also depended on the tissue used and in all cases decreased with rising environmental temperature. These two facts are taken to reflect interactions between acetylcholinesterase and membrane that depend on the nature of the membrane and probably involve the aliphatic chains of its phospholipids. The greater case of solubilization found in the low temperature adapted fish may be a function of the increased fluidity of the cellular membranes formed at lower temperatures.
journal_name
Biochimiejournal_title
Biochimieauthors
Guillon G,Massoulié J,Guillon G,Massoulié Jdoi
10.1016/s0300-9084(76)80257-xsubject
Has Abstractpub_date
1976-01-01 00:00:00pages
465-71issue
4eissn
0300-9084issn
1638-6183pii
S0300-9084(76)80257-Xjournal_volume
58pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Beta-turmerin from turmeric (Curcuma longa) waste grits obtained after extraction of curcumin was purified by successive gel permeation chromatography. Homogeneity of beta-turmerin was confirmed by its movement as single band both in SDS-PAGE and as well as in native (basic) PAGE. The apparent molecular mass is approx...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2009.06.006
更新日期：2009-09-01 00:00:00
abstract：:Estradiol-PAB-cellulose, an easily prepared adsorbent, has been proposed to purify the uterine estradiol receptor according to the principle of biospecific affinity chromatography. It apparently removes all hormone binding sites when cytosol preparations are incubated with it. A systematic study of this adsorbent was ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80317-3
更新日期：1976-01-01 00:00:00
abstract：:Nitroxyl (HNO) is a molecule of significant interest due to its unique pharmacological properties, particularly within the cardiovascular system. A large portion of HNO biological effects can be attributed to its reactivity with protein thiols, where it can generate disulfide bonds. Evidence from studies in erythrocyt...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.12.005
更新日期：2012-03-01 00:00:00
abstract：:When liver cells from either normal or hereditarily diabetic rats are exposed to (13)C-enriched D-fructose (10 mM) and unlabelled D-glucose (also 10 mM) in the presence of D(2)O, the output of (13)C-enriched D-glucose generated from D-[1-(13)C]fructose is significantly lower than that from D-[2-(13)C]fructose. This co...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2003.12.005
更新日期：2004-02-01 00:00:00
abstract：:Leishmania parasites cause two million new cases of leishmaniasis each year with several hundreds of millions of people at risk. Due to the paucity and shortcomings of available drugs, we have undertaken the crystal structure determination of a key enzyme from Leishmania major in hopes of creating a platform for the r...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.11.015
更新日期：2011-03-01 00:00:00
abstract：:Epidemiological studies have demonstrated that the diabetes mellitus is a serious health burden for both governments and healthcare providers. The present study was hypothesized to evaluate the antihyperglycemic potential of fraxetin by determining the activities of key enzymes of carbohydrate metabolism in streptozot...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.06.013
更新日期：2013-10-01 00:00:00
abstract：:Endothelial nitric oxide synthase (eNOS) plays a crucial role in the regulation of a variety of cardiovascular functions. Many studies have shown that dietary n-3 polyunsaturated fatty acids (PUFAs) have beneficial effects on coronary atherosclerosis. However, the mechanisms of n-3 PUFAs regulation in eNOS activation ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2006.10.009
更新日期：2007-01-01 00:00:00
abstract：:Lactic acid bacteria provide a model system for the in vivo study of mechanisms pertaining to the regulation of sugar transport and metabolism by microorganisms. Recent studies with resting and growing cells of the homofermentative Streptococci and Lactobacilli have yielded evidence for hitherto unsuspected regulatory...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(88)90205-2
更新日期：1988-03-01 00:00:00
abstract：:Rhodospirillum salexigens is a moderately halophilic purple phototrophic bacterium which grows optimally in 8% NaCl. The amino acid sequences of the two principal soluble cytochromes c have been determined. One of these is a cytochrome c2, similar in size to mitochondrial cytochrome c. While clearly of the same sequen...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(94)90135-x
更新日期：1994-01-01 00:00:00
abstract：:We identified a T-to-C mutation 2 nucleotides (nt) upstream from the AG in a GT-AG intron between exons 2 and 3 in the human T-cell leukemia virus type I (HTLV-I) tax mRNA. This mutation resulted in the preferential usage of an alternative splice site, causing a 75-nt elongation of tax mRNA and reduced production of v...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.07.013
更新日期：2006-02-01 00:00:00
abstract：:Phosphatidic acid phosphohydrolase-1 (PAP-1) activity is reversibly inhibited by fatty acids and their acyl-CoA esters and it appears paradoxical that these effectors have been reported to increase the liver's esterification capacity by translocating the rate-limiting enzyme PAP-1 from cytosol to the endoplasmic retic...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.01.003
更新日期：2005-05-01 00:00:00
abstract：:Previous reports on the carbohydrate specificities of Amaranthus caudatus lectin (ACL) and peanut agglutinin (PNA, Arachis hypogea) indicated that they share the same specificity for the Thomsen-Friedenreich (T(alpha), Galbeta1-3GalNAcalpha1-Ser/Thr) glycotope, but differ in monosaccharide binding--GalNAc>Gal (inactiv...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2008.08.001
更新日期：2008-11-01 00:00:00
abstract：:Betaine aldehyde dehydrogenase from the human opportunistic pathogen Pseudomonas aeruginosa (PaBADH) catalyzes the irreversible, NAD(P)(+)-dependent oxidation of betaine aldehyde, producing glycine betaine, an osmoprotectant. PaBADH participates in the catabolism of choline and likely in the defense against the osmoti...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.09.022
更新日期：2011-02-01 00:00:00
abstract：:The functions of residues 57-RY-58, G60, L77, 80-GSGR-83, I101, T104, 134-GY-135, N155, V157 and 160-LV-161 in human arsenic (III) methyltransferase (hAS3MT) 5 Å around S-adenosylmethionine (SAM) have not been studied. Herein, sixteen mutants were designed by substituting these residues with Ala. Mutants G60A, G80A, I...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.10.014
更新日期：2014-12-01 00:00:00
abstract：:The oncogenic potential of the high-risk human papillomavirus (HPV) relies on the expression of genes specifying the E7 and E6 proteins. To investigate further the variation in oligomeric structure that has been reported for different E7 proteins, an HPV-18 E7 cloned from a Hispanic woman with cervical intraepithelial...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.02.028
更新日期：2010-07-01 00:00:00
abstract：:MicroRNAs (miRNAs) are ∼21 nt non-coding small RNAs which regulate gene expression at the post-transcriptional level in plants and animals. Until recently, only limited numbers of miRNAs were identified in Cucurbitaceae, a large flowering plant family. In this study, 220 potential miRNA candidates were identified from...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.03.002
更新日期：2014-07-01 00:00:00
abstract：:The efficiency of oxidative phosphorylation was estimated in intact resting cells of Escherichia coli K 12, strain PA 601 (chl-s) and its chl-r mutants, all of them grown anaerobically in the presence of nitrate. The oxidation of endogenous NADH in intact chl-s cells was accompanied by the formation of ATP whatever th...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(77)80316-7
更新日期：1977-01-01 00:00:00
abstract：:The effects of thermal injury (72 h post-injury), 72 h-partial (20% less food) or full starvation on the regulation of phosphofructokinase in the mucosa of rat small intestine were studied. Thermal injury and 72 h-partial or full starvation decreased the activity ratio v0.5/V, but the ratios obtained for thermally inj...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(87)90273-2
更新日期：1987-01-01 00:00:00
abstract：:Insulin stimulates protein synthesis in skeletal muscle of young animals and has been reported to exert similar effects on a variety of mammalian cell types in culture. However, with chick embryo fibroblasts we found that the extent of this effect was very sensitive to the culture conditions of the cells prior to the ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(93)90129-g
更新日期：1993-01-01 00:00:00
abstract：:A major incentive in inhibitor research is that control of limited proteolysis constitutes a valuable pharmacological tool. Protease inhibitors have proved to be successful in influencing pathogenesis in many experimental models but a breakthrough to use in human therapy has mainly been restricted to aprotinin and ang...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(91)90084-e
更新日期：1991-01-01 00:00:00
abstract：:Zika and Dengue viruses have attracted substantial attention from researchers in light of recent outbreaks of Dengue fever and increases in cases of congenital microcephaly in areas with Zika incidence. This review summarizes the current state of knowledge about Zika and Dengue proteases. These enzymes have several in...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2019.05.004
更新日期：2019-11-01 00:00:00
abstract：:Synthetic antiestrogens such as Tamoxifen (Nolvadex) are currently used to treat postmenopausal breast cancer. Their mechanism of action is reviewed at the cellular and molecular level, through data of the literature and the current view of the author. Synthetic antiestrogens are mostly acting directly on breast cance...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(82)80411-2
更新日期：1982-02-01 00:00:00
abstract：:Posttranscriptional gene regulation by small RNAs and its crucial impact on development, apoptosis, stem cell self-renewal and differentiation gained tremendous scientific attention since the discovery of RNA interference (RNAi) and microRNAs (miRNAs). However, in the last few years, many more examples for regulatory ...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2011.07.032
更新日期：2011-11-01 00:00:00
abstract：:Synthesis of the tripeptide Z-Phe-Arg-SerNH2 has been accomplished by a recombinant cysteine protease, cathepsin L1 from liver fluke (Fasciola hepatica), using Z-Phe-Arg-OMe as acyl acceptor and SerNH2 as nucleophile in 0.1 M ammonium acetate pH 9.0-12.5% v/v acetonitrile at 37 degrees C. LC-MS detection indicated tri...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2005.06.004
更新日期：2006-01-01 00:00:00
abstract：:While many works analyse lipid composition of cellular nuclei, essentially of liver, few describe bird erythrocyte nuclei. For them very discordant results are reported due to very different methods of preparation. In the method we adopted a good reproducibility is controlled by electron microscopy, evaluation of spec...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80126-5
更新日期：1976-01-01 00:00:00
abstract：:The Escherichia coli protein synthesis initiation factor IF2 is a member of the large family of G-proteins. Along with translational elongation factors EF-Tu and EF-G and translational release factor RF-3, IF2 belongs to the subgroup of G-proteins that are part of the prokaryotic translational apparatus. The roles of ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(00)01200-1
更新日期：2000-12-01 00:00:00
abstract：:Diacylglycerol kinase (DGK) phosphorylates diacylglycerol to produce phosphatidic acid (PA). The η isozyme of DGK is abundantly expressed in C2C12 myoblasts. However, the role of DGKη in skeletal muscle cells remains unknown. In the present study, we showed that DGKη was downregulated at an early stage of myogenic dif...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.07.018
更新日期：2020-10-01 00:00:00
abstract：:Using a rapid FPLC procedure for the separation of calpain I, calpain II and the inhibitor calpastatin from pork meat, results of proteolytic and inhibitory activity were obtained. Duplicate measurements showed variation coefficients less than 0.13. Compared to others we found lower calpain I activity 1 h post-mortem....
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(93)90041-p
更新日期：1993-01-01 00:00:00
abstract：:The lipoprotein complex from human gallbladder bile was challenged with anti-IgA and anti-IgG antisera in order to determine whether the apolipoprotein complex isolated from the detergent-free form of bile lipoprotein contains IgA and IgG. The apolipoprotein complex indeed crossreacts with anti-IgA and anti-IgG. In ad...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(81)80033-8
更新日期：1981-10-01 00:00:00
abstract：:Leptin is a cytokine secreted by the adipose tissue that is involved in the control of body weight. We previously showed that a point mutation (R105W) in leptin results in leptin deficiency, marked obesity and hypogonadism in humans adults. Expression in COS1 cells showed impaired secretion and intracellular accumulat...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2004.06.005
更新日期：2004-06-01 00:00:00
