Abstract:
:When liver cells from either normal or hereditarily diabetic rats are exposed to (13)C-enriched D-fructose (10 mM) and unlabelled D-glucose (also 10 mM) in the presence of D(2)O, the output of (13)C-enriched D-glucose generated from D-[1-(13)C]fructose is significantly lower than that from D-[2-(13)C]fructose. This coincides with a higher generation of (13)C-enriched L-lactate and L-alanine from D-[1-(13)C]fructose, as compared to D-[2-(13)C]fructose. In absolute terms, the mean paired difference in the output of (13)C-enriched D-glucose generated from D-[1-(13)C]fructose versus D-[2-(13)C]fructose is not significantly different from the mean paired difference in the production of (13)C-enriched L-lactate and L-alanine from the same precursors, with an overall mean value of 7.01 +/- 1.59 micromol (n = 8; P < 0.005). It is proposed that these findings indicate isotopic discrimination at the phosphoglucoisomerase level between (12)C and (13)C for the carbon atom in position 1 (as compared to that in position 2) of D-fructose 6-phosphate.
journal_name
Biochimiejournal_title
Biochimieauthors
Malaisse WJ,Willem Rdoi
10.1016/j.biochi.2003.12.005keywords:
subject
Has Abstractpub_date
2004-02-01 00:00:00pages
119-25issue
2eissn
0300-9084issn
1638-6183pii
S0300908404000045journal_volume
86pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Poly(ADP-ribosyl)ation is a posttranslational protein modification significant for genomic stability and cell survival in response to DNA damage. Poly(ADP-ribosyl)ation is catalyzed by poly(ADP-ribose)polymerases (PARPs). Among the 17 members of the PARP family, PARP-1 and PARP-2 are described as enzymes whose catalyt...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.01.007
更新日期:2013-06-01 00:00:00
abstract::Unlike most tonoplast ATPases, the vacuolar ATPase of Acer pseudoplatanus cells (Km = 0.4 mM) was strongly inhibited by vanadate (I50 = 10 microM). The inhibition was non-competitive. Chemicals usually added in the reaction mixture either increase (NH+4, K+) or decrease (Na+, EDTA) the ATPase inhibition. However, thes...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(89)90044-8
更新日期:1989-07-01 00:00:00
abstract::Antimicrobials targeting the fatty acid synthesis (FASII) pathway are being developed as alternative treatments for bacterial infections. Emergence of resistance to FASII inhibitors was mainly considered as a consequence of mutations in the FASII target genes. However, an alternative and efficient anti-FASII resistanc...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2017.07.004
更新日期:2017-10-01 00:00:00
abstract::As part of a project aimed at obtaining selective inhibitors and drug-like compounds targeting tRNA synthetases from trypanosomatids, we have elucidated the crystal structure of human cytosolic histidyl-tRNA synthetase (Hs-cHisRS) in complex with histidine in order to be able to compare human and parasite enzymes. The...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.08.005
更新日期:2014-11-01 00:00:00
abstract::Cryptogein, a 98 amino acid protein secreted by the fungus Phytophthora cryptogea, induces a hypersensitive response and systemic acquired resistance in tobacco plants (Nicotiana tabacum var Xanthi). The mode of action of cryptogein has been studied using tobacco cell suspensions. The recognition of this elicitor by a...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(99)80123-0
更新日期:1999-06-01 00:00:00
abstract::Pulmonary fibrosis (PF) is a progressive lethal disorder. In this study, the effect of daidzein, a soyisoflavone against Bleomycin (BLM) induced PF in rats was elucidated. A single intratracheal instillation of BLM (3 U/kg.bw) was administered in rats to induce PF. Daidzein (0.2 mg/kg) was administered subcutaneously,...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.04.005
更新日期:2014-08-01 00:00:00
abstract::Dysfunction in lipid metabolism may result in a decrease in hepatic autophagy, which contributes to the pathogenesis of non-alcoholic steatohepatitis. ATP-binding cassette transporter A1 transports free cholesterol and phospholipids to apolipoprotein A-I (apoA-I) to form nascent high-density lipoprotein particles. Res...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.08.001
更新日期:2019-10-01 00:00:00
abstract::Apolipoprotein E (apoE) has been genetically linked to late-onset Alzheimer's disease. From the three common alleles (epsilon2, epsilon3 and epsilon4), epsilon4 has been suggested to promote amyloid beta (Ass) plaque fibrillation, one hallmark of Alzheimer's disease. It has been demonstrated that altered lipid content...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.10.007
更新日期:2006-05-01 00:00:00
abstract::The recF gene of E coli lies within a cluster of genes which play essential roles in DNA replication; the gene order is dnaA dnaN recF gyrB. Each of these genes has its own promoters which, with the exception of dnaA promoters, reside entirely within the translated region of the respective preceding gene. In this repo...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90220-u
更新日期:1991-02-01 00:00:00
abstract::Latices from Asclepias spp are used in wound healing and the treatment of some digestive disorders. These pharmacological actions have been attributed to the presence of cysteine proteases in these milky latices. Asclepias curassavica (Asclepiadaceae), "scarlet milkweed" is a perennial subshrub native to South America...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.07.017
更新日期:2009-11-01 00:00:00
abstract::The issue of whether histone H1 possesses specificity of binding to certain nucleotide sequences in DNA is of fundamental importance to the suggested role of the linker histone in the regulation of gene transcription. The purpose of the present study was to reinvestigate the specificity of binding of histone H1 to the...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(93)90116-a
更新日期:1993-01-01 00:00:00
abstract::Regeneration is an adaptive phenomenon with wide biological implications spread heterogeneously in almost all the organism including human beings. The ability of regeneration varies from species to species for its complexity. Epimorphic regeneration of zebrafish caudal fin tissue is the most widely studied regeneratio...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.05.014
更新日期:2020-08-01 00:00:00
abstract::Isopentenyl adenosine derivatives can be found next to the anticodon (position 37) in tRNA from both the Bacteria and Eucarya domains. These modified nucleosides improve the efficiency of tRNA in translation, can increase and decrease translational fidelity, and make the tRNA less codon context sensitive. In bacteria ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(94)90044-2
更新日期:1994-01-01 00:00:00
abstract::Matrix metalloproteinase 9 (MMP-9) and interleukin-8 (IL-8) play major roles in tumor progression and invasion of breast cancer cells. The present study was undertaken to investigate the inhibitory mechanism of cell invasion by luteolin 8-C-β-fucopyranoside (named as LU8C-FP), a C-glycosylflavone, in human breast canc...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.07.021
更新日期:2013-11-01 00:00:00
abstract::Potato virus A (PVA), a potyvirus with a (+)ssRNA genome translated to a large polyprotein, was engineered and used as a gene vector for expression of heterologous proteins in plants. Foreign genes including jellyfish GFP (Aequorea victoria) encoding the green fluorescent protein (GFP, 27 kDa) and the genes of human o...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.10.010
更新日期:2006-05-01 00:00:00
abstract::The specific binding of the amyloid protein precursor (APP) to glycosaminoglycans (GAG) suggests that APP is a cell adhesion molecule (CAM) and/or substrate adhesion molecule (SAM). In order to characterize this activity of APP in the brain at the molecular level, we have purified and characterized the major APP speci...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(94)90163-5
更新日期:1994-01-01 00:00:00
abstract::The poisonous bite by Hippasa partita, a funnel web spider from the Indian subcontinent has been demonstrated to give rise to severe dermo- and myonecrosis. In this work a hemorrhagic metalloprotease, Partitagin was purified from H. partita venom by successive chromatography on Sephadex G-100, DEAE Sephadex A-50 and B...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2007.04.005
更新日期:2007-11-01 00:00:00
abstract::We identified a T-to-C mutation 2 nucleotides (nt) upstream from the AG in a GT-AG intron between exons 2 and 3 in the human T-cell leukemia virus type I (HTLV-I) tax mRNA. This mutation resulted in the preferential usage of an alternative splice site, causing a 75-nt elongation of tax mRNA and reduced production of v...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2005.07.013
更新日期:2006-02-01 00:00:00
abstract::Microsomal membranes from potato tubers were extracted by acetone solutions of increasing concentrations (5, 10, 15, 20, 30, 40, 50, 70 and 90 p. cent). Microsomal lipids were progressively extracted: acetone concentrations exceeding 30 p. cent extracted large amounts of membraneous phospholipids (figure). Lipid extra...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(78)80021-2
更新日期:1978-01-01 00:00:00
abstract::Genome-editing technologies, in particular, CRISPR systems, are widely used for targeted regulation of gene expression and obtaining modified human and animal cell lines, plants, fungi, and animals with preassigned features. Despite being well described and easy to perform, the most common methods for construction and...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2019.09.003
更新日期:2019-12-01 00:00:00
abstract::In vertebrates, mannose 6-phosphate receptors [MPR300 (Mr 300 kDa) and MPR46 (Mr 46 kDa)] are highly conserved transmembrane glycoproteins that mediate transport of lysosomal enzymes to lysosomes. Our studies have revealed the appearance of these putative receptors in invertebrates such as the molluscs and deuterostom...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.02.007
更新日期:2012-05-01 00:00:00
abstract::The proton NMR data of a synthetic model of deoxy-myoglobin and hemoglobin show one that:(i) the ground state of the high spin iron (II) complex must be described by at least two electronic levels separated by ca.200 cm-1 and resulting from the removal of the degeneracy of the 5E level; (ii) this in-plane rhombic dist...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(82)80285-x
更新日期:1981-11-01 00:00:00
abstract::The adaptive response appears in E. coli after exposure to low levels of alkylating agents. This system is under the positive control of the ada gene. At least two enzymes are induced during the response: 3-methyladenine DNA glycosylase II and O6-methylguanine DNA methyltransferase. The latter is also the product of t...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(85)80080-8
更新日期:1985-03-01 00:00:00
abstract::OURCIN is a tool to build expert systems, which was developed by a joint team from INRIA and SEMA. We emphasized developing on this system the ergonomic features which make Expert Systems a comfortable and interesting approach for knowledge engineering. ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(85)80267-4
更新日期:1985-05-01 00:00:00
abstract::In order to study the relationship between bile cholesterol and free cholesterol carried by high and low density lipoproteins (HDL and LDL), 10 male Wistar rats, 11 weeks old and fed with a standard diet were divided into 3 groups which received an intravenous infusion (jugular vein) of either LDL, HDL or liposomes. L...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(87)90270-7
更新日期:1987-01-01 00:00:00
abstract::Estradiol-PAB-cellulose, an easily prepared adsorbent, has been proposed to purify the uterine estradiol receptor according to the principle of biospecific affinity chromatography. It apparently removes all hormone binding sites when cytosol preparations are incubated with it. A systematic study of this adsorbent was ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80317-3
更新日期:1976-01-01 00:00:00
abstract::Colorectal cancer (CRC) is the third most diagnosed cancer in the western world, affecting 1 out of approximately 22 people in their lifetime. Several epidemiological studies suggest a positive association between high plasma cholesterol levels and colorectal cancer. However, the molecular mechanisms by which choleste...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.07.006
更新日期:2018-10-01 00:00:00
abstract::IL-28 and IL-29 were recently described as members of a new cytokine family that shares with type I interferon (IFN) the same Jak/Stat signalling pathway driving expression of a common set of genes. Accordingly, they have been named IFN lambda. IFNs lambda exhibit several common features with type I IFNs: antiviral ac...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2007.01.008
更新日期:2007-06-01 00:00:00
abstract::The physical maps of cloned recBCD gene regions of Serratia marcescens and Proteus mirabilis were correlated to genes located in this region. The genes thyA, recC, recB, recD and argA were organized as in Escherichia coli. The 3 rec genes code for the 3 different subunits of the RecBCD enzyme and produced enzymes prom...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(91)90104-9
更新日期:1991-04-01 00:00:00
abstract::Lysophosphatidic acid (LPA), a naturally occurring bioactive phospholipid, mediates a multitude of (patho)physiological events including activation of mitogen-activated protein kinases (MAPKs). As LPA may induce cellular reponses in human osteosarcoma, the present study aimed at investigating expression of various LPA...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.05.023
更新日期:2012-09-01 00:00:00