Abstract：
:Estradiol-PAB-cellulose, an easily prepared adsorbent, has been proposed to purify the uterine estradiol receptor according to the principle of biospecific affinity chromatography. It apparently removes all hormone binding sites when cytosol preparations are incubated with it. A systematic study of this adsorbent was undertaken, including the synthesis and testing of the radioactive material. Two main results were obtained: 1) Estradiol-PAB-cellulose is heavily contaminated with free ligand and releases it during the normal chromatographic conditions. 2) Estradiol spacer derivatives (hydroxyethylphenyl-diazo (2 or 4)-estradiol) have a very low affinity for the receptor (Ki = 10 muM). The conclusion is that estradiol-PAB-cellulose is unsuitable for affinity chromatography of estradiol receptor.
journal_name
Biochimiejournal_title
Biochimieauthors
Best-Belpomme M,Richard-Foy H,Secco-Millet C,Baulieu EEdoi
10.1016/s0300-9084(76)80317-3keywords:
subject
Has Abstractpub_date
1976-01-01 00:00:00pages
863-9issue
7eissn
0300-9084issn
1638-6183pii
S0300-9084(76)80317-3journal_volume
58pub_type
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