Abstract:
:The regulation of transcription of the pckA gene coding for phosphoenolpyruvate carboxykinase in Escherichia coli was analysed by site-directed mutagenesis of the promoter region and measurement of in vitro transcription initiation. Mutation of the guanine residue at position -14 to either cytosine or thymine was found to result in a drastic decrease of transcription, even in the presence of the natural -35 hexamer TTTCCA that differs by only two nucleotides from the consensus sequence TTGACA. It was concluded that the promoter region of pckA contains an extended -10 module, 5'-TG-3', located one base upstream of the -10 hexamer, which is crucial for transcription.
journal_name
Biochimiejournal_title
Biochimieauthors
Prost JF,Cozzone AJdoi
10.1016/s0300-9084(99)80052-2keywords:
subject
Has Abstractpub_date
1999-03-01 00:00:00pages
197-200issue
3eissn
0300-9084issn
1638-6183pii
S0300-9084(99)80052-2journal_volume
81pub_type
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