Abstract：
:Maximum 125I-IGF-I/Sm-C total binding to chick embryo fibroblasts was 3% at +37 degrees C and decreased to less than 1% in presence of 2.8 X 10(-9) M unlabelled IGF-I/Sm-C. Insulin did not compete with IGF-I/Sm-C for the binding to cells. Biological action of IGF-I/Sm-C was evaluated on 2-deoxyglucose and alpha-aminoisobutyrate uptake. Results are compared with those obtained with insulin. Maximal peptide effects on the two transport processes were obtained at a 0.65 X 10(-7) M concentration and for a 120 minute association time, whereas cells were markedly less sensitive to insulin and time response curves were different. These results suggest that insulin action on nutrient uptake in chick embryo fibroblasts is not mediated by the binding of the hormone to IGF-I/Sm-C receptors.
journal_name
Biochimiejournal_title
Biochimieauthors
Cynober L,Aussel C,Chatelain P,Vaubourdolle M,Agneray J,Ekindjian OGdoi
10.1016/s0300-9084(85)80118-8subject
Has Abstractpub_date
1985-10-01 00:00:00pages
1185-90issue
10-11eissn
0300-9084issn
1638-6183pii
S0300-9084(85)80118-8journal_volume
67pub_type
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