Inhibitors of specific ceramide synthases.

abstract：:Snake venom Kunitz-type serine protease inhibitors (KSPIs) exhibit various biological functions including anticoagulant activity. This study elucidates the occurrence and subunit stoichiometry of a putative complex formed between two KSPIs (Rusvikunin and Rusvikunin-II) purified from the native Rusvikunin complex of P...

journal_title：Biochimie

authors： Mukherjee AK,Dutta S,Kalita B,Jha DK,Deb P,Mackessy SP

更新日期：2016-09-01 00:00:00

abstract：:Anaerobiospirillum succiniciproducens phosphoenolpyruvate (PEP) carboxykinase catalyses the reversible metal-dependent formation of oxaloacetate (OAA) and ATP from PEP, ADP and CO(2). Mutations of PEP carboxykinase have been constructed where the residues His(225) and Asp(263), two residues of the enzyme's putative Mn...

journal_title：Biochimie

authors： Jabalquinto AM,González-Nilo FD,Laivenieks M,Cabezas M,Zeikus JG,Cardemil E

更新日期：2004-01-01 00:00:00

abstract：:After exhaustive pronase digestion, purification by gel filtration and affinity chromatography on concanavalin A, three glycopeptide fractions were obtained from rat hemopexin. Two fractions (I and II) were concanavalin A non-reactive and one (III) was concanavalin A reactive. On the basis of carbohydrate composition,...

journal_title：Biochimie

authors： Bernard N,Lombart C,Strecker G,Montreuil J,Van Halbeek H,Vliegenthart JF

更新日期：1983-03-01 00:00:00

abstract：:The eukaryotic genome is compacted in the cell nucleus, in a way that allows its faithful and ordered replication each cell cycle. Chromatin is organized into topologically constrained loops that are anchored to the nuclear matrix by specific attachment regions (SARs). Chromatin loops were proposed to correspond to re...

journal_title：Biochimie

authors： Hyrien O,Maric C,Lucas I

更新日期：1997-10-01 00:00:00

abstract：:Bacterial type II fatty acid synthesis (FASII) is a target for novel antibiotic development. All bacteria encode for mechanisms to incorporate exogenous fatty acids, and some bacteria can use exogenous fatty acids to bypass FASII inhibition. Bacteria encode three different mechanisms for activating exogenous fatty aci...

journal_title：Biochimie

authors： Yao J,Rock CO

更新日期：2017-10-01 00:00:00

abstract：:The functions of residues 57-RY-58, G60, L77, 80-GSGR-83, I101, T104, 134-GY-135, N155, V157 and 160-LV-161 in human arsenic (III) methyltransferase (hAS3MT) 5 Å around S-adenosylmethionine (SAM) have not been studied. Herein, sixteen mutants were designed by substituting these residues with Ala. Mutants G60A, G80A, I...

journal_title：Biochimie

authors： Li X,Geng Z,Chang J,Song X,Wang Z

更新日期：2014-12-01 00:00:00

abstract：:Two forms of baker's yease valyl-tRNA synthetase have been purified to apparent homogeneity by classical methods. It was demonstrated that one of the two forms of the enzyme originates from the other by proteolysis, the respective amounts of each form depending on the physiological state of the yeast. The species main...

journal_title：Biochimie

authors： Kern D,Giegé R,Robre-Saul S,Boulanger Y,Ebel JP

更新日期：1975-01-01 00:00:00

abstract：:Seven virulent bacteriophages of Streptococcus thermophilus were characterized at the molecular level and classified into 2 subgroups (A and B) by DNA/DNA hybridization experiments and analysis of their structural proteins. Two representatives of subgroups A and B were compared to 3 representatives of Neve's subgroups...

journal_title：Biochimie

authors： Benbadis L,Faelen M,Slos P,Fazel A,Mercenier A

更新日期：1990-12-01 00:00:00

abstract：:Extracellular Hsp70 (eHsp70) can activate immune cells via Toll-like receptors (TLR) 2 and 4, and induce cytokine synthesis. The aim of this study was to explore inflammation-associated effects of eHsp70 alone and in combination with cigarette smoke extract (CSE) in primary bronchial epithelial cells. We assessed IL-6...

journal_title：Biochimie

authors： Hulina-Tomašković A,Heijink IH,Jonker MR,Somborac-Bačura A,Grdić Rajković M,Rumora L

更新日期：2019-01-01 00:00:00

abstract：:Isolation and properties of B. subtilis ts 19 mutant, isolated as thermosensitive for sporulation, are described. At the non permissive temperature (42degreesC), the mutant cells are blocked at stage zero of sporulation and do not excrete extracellular enzymes such as serylprotease and esterase. At the permissive temp...

journal_title：Biochimie

authors： Millet J,Larribe M,Aubert JP

更新日期：1976-01-01 00:00:00

abstract：:In this review, we show how the stability of the asymmetric transverse distribution of phospholipids and the physiological role of the asymmetric distribution can be explained. Experiments with paramagnetic or fluorescent lipids enabled us to show that in fresh red blood cells, i.e. containing ATP, and in resealed gho...

journal_title：Biochimie

authors： Devaux PF,Zachowski A,Favre E,Fellmann P,Cribier S,Geldwerth D,Hervé P,Seigneuret M

更新日期：1986-03-01 00:00:00

abstract：:Structural and motional features in the denatured state of a protein dictate the early folding events starting from that state and these features vary depending upon the nature of the denaturant used. Here, we have attempted to decipher the early events in the folding of Dynein Light Chain protein (DLC8), starting fro...

journal_title：Biochimie

authors： Chakraborty S,Mohan PM,Hosur RV

更新日期：2012-01-01 00:00:00

abstract：:Replication protein A (RPA) is a heterotrimeric single-stranded DNA binding protein whose role in DNA replication, recombination and repair has been mainly elucidated through in vitro biochemical studies utilizing the mammalian complex. However, the identification of homologs of all three subunits in Saccharomyces cer...

journal_title：Biochimie

authors： Smith J,Zou H,Rothstein R

更新日期：2000-01-01 00:00:00

abstract：:Carbonic anhydrases (CAs, EC 4.2.1.1) are metalloenzymes found throughout the phylogenetic tree. The β-class carbonic anhydrases (β-CAs) are the predominating class of CAs in plants. Growing evidence underscores the importance of β-CAs in plant immunity and environmental adaptation in addition to their roles in photos...

journal_title：Biochimie

authors： Wang M,Zhang Q,Liu FC,Xie WF,Wang GD,Wang J,Gao QH,Duan K

更新日期：2014-02-01 00:00:00

abstract：:Embryonic stem cells (ESCs) are capable of unlimited self-renewal and differentiation into multiple cell types. Recent large-scale analyses have identified various cell surface molecules on ESCs. Some of them are considered to be beneficial markers for characterization of cellular phenotypes and/or play an essential r...

journal_title：Biochimie

authors： Iwagawa T,Ohuchi SP,Watanabe S,Nakamura Y

更新日期：2012-01-01 00:00:00

abstract：:The hypothesis is proposed that pre-biotic bacterial cell(s) and the first cells capable of growth/division did not require a cytoplasmic membrane. A gel-like microscopic structure less than a cubic micrometer may have had a dual role as both an ancient pre-cytoplasm and a boundary layer to the higher-entropy external...

journal_title：Biochimie

authors： Trevors JT,Pollack GH

更新日期：2012-01-01 00:00:00

abstract：:Chronic hypobaric hypoxia induced muscle atrophy results in decreased physical performance at high altitude. Curcumin has been shown to have muscle sparing effects under cachectic conditions. However, the protective effects of curcumin under chronic hypobaric hypoxia have not been studied till now. Therefore, the pres...

journal_title：Biochimie

authors： Chaudhary P,Sharma YK,Sharma S,Singh SN,Suryakumar G

更新日期：2019-01-01 00:00:00

abstract：:Diadenosine 5',5'''-p1,p4-tetraphosphate (Ap4A) alpha, beta-phosphorylase from yeast Saccharomyces cerevisiae catalyzes two reactions: Ap4A cleavage and nucleoside diphosphate--phosphate (NDP-Pi) exchange. In both reactions phosphate can be substituted by arsenate, chromate, molybdate, tungstate or vanadate. In the pr...

journal_title：Biochimie

authors： Guranowski A,Blanquet S

更新日期：1986-05-01 00:00:00

abstract：:Cytochromes P450 constitute a superfamily of hemoproteins which have evolved from a common ancestor. Recent advances in molecular biology have offered a powerful approach to the research on the multiplicity of P450. Now, every mammalian P450 species can be characterized by heterologous expression of its cDNA. In addit...

journal_title：Biochimie

authors： Yabusaki Y

更新日期：1995-01-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) and αvβ3 integrin are key molecules that actively participate in tumor angiogenesis and metastasis. Some integrin-blocking molecules are currently under clinical trials for cancer and metastasis treatment. However, the mechanism of action of such inhibitors is not completely u...

journal_title：Biochimie

authors： Montenegro CF,Salla-Pontes CL,Ribeiro JU,Machado AZ,Ramos RF,Figueiredo CC,Morandi V,Selistre-de-Araujo HS

更新日期：2012-08-01 00:00:00

abstract：:An efficient phosphotriester methodology based on the use of condensing agents in the presence of several O-nucleophilic catalysts has been developed. ...

journal_title：Biochimie

authors： Efimov VA,Chakhmakhcheva OG

更新日期：1985-07-01 00:00:00

abstract：:The innate immunity factor lactoferrin harbours two antimicrobial moieties, lactoferricin and lactoferrampin, situated in close proximity in the N1 domain of the molecule. Most likely they cooperate in many of the beneficial activities of lactoferrin. To investigate whether chimerization of both peptides forms a funct...

journal_title：Biochimie

authors： Bolscher JG,Adão R,Nazmi K,van den Keybus PA,van 't Hof W,Nieuw Amerongen AV,Bastos M,Veerman EC

更新日期：2009-01-01 00:00:00

abstract：:Genome-editing technologies, in particular, CRISPR systems, are widely used for targeted regulation of gene expression and obtaining modified human and animal cell lines, plants, fungi, and animals with preassigned features. Despite being well described and easy to perform, the most common methods for construction and...

journal_title：Biochimie

authors： Filippova J,Matveeva A,Zhuravlev E,Stepanov G

更新日期：2019-12-01 00:00:00

abstract：:Concern about the malicious applications of botulinum neurotoxin has highlighted the need for a new generation of safe and highly potent antitoxins. In this study, we developed and evaluated the preclinical pharmacology and safety of a new F(ab')₂ antitoxin against botulinum neurotoxin serotype A (BoNT/A). As an alter...

journal_title：Biochimie

authors： Yu YZ,Zhang SM,Wang WB,Du Y,Zhu HQ,Wang RL,Zhou XW,Lin JB,Wang S,Yu WY,Huang PT,Sun ZW

更新日期：2010-10-01 00:00:00

abstract：:Unlike most tonoplast ATPases, the vacuolar ATPase of Acer pseudoplatanus cells (Km = 0.4 mM) was strongly inhibited by vanadate (I50 = 10 microM). The inhibition was non-competitive. Chemicals usually added in the reaction mixture either increase (NH+4, K+) or decrease (Na+, EDTA) the ATPase inhibition. However, thes...

journal_title：Biochimie

authors： Montrichard F,Pugin A,Gaudemer Y

更新日期：1989-07-01 00:00:00

abstract：:Low bone formation in osteoporosis is associated with a shift from osteoblastic to adipogenic differentiation of mesenchymal stem cells (MSC) inducing a concomitant lipotoxic milieu within the bone marrow. Strontium ranelate (SrRN), a treatment for osteoporosis, has both anti-resorptive and anabolic effects on bone. T...

journal_title：Biochimie

authors： Vidal C,Gunaratnam K,Tong J,Duque G

更新日期：2013-04-01 00:00:00

abstract：:Poly(ADP-ribosyl)ation is a posttranslational protein modification significant for genomic stability and cell survival in response to DNA damage. Poly(ADP-ribosyl)ation is catalyzed by poly(ADP-ribose)polymerases (PARPs). Among the 17 members of the PARP family, PARP-1 and PARP-2 are described as enzymes whose catalyt...

journal_title：Biochimie

authors： Kutuzov MM,Khodyreva SN,Amé JC,Ilina ES,Sukhanova MV,Schreiber V,Lavrik OI

更新日期：2013-06-01 00:00:00

abstract：:Here we report two novel 17-mer amidated linear peptides (TsAP-1 and TsAP-2) whose structures were deduced from cDNAs cloned from a venom-derived cDNA library of the Brazilian yellow scorpion, Tityus serrulatus. Both mature peptides were structurally-characterised following their location in chromatographic fractions ...

journal_title：Biochimie

authors： Guo X,Ma C,Du Q,Wei R,Wang L,Zhou M,Chen T,Shaw C

更新日期：2013-09-01 00:00:00

abstract：:We present here, a new method for coupling sulfhydryl groups (SH) to low-density lipoprotein (LDL) surface. This method uses homocysteine thiolactone (HCTL) which reacts with lysine residues in a very mild manner, and permits the selection of the number of SH bound per LDL. Under our experimental conditions (8 SH/LDL)...

journal_title：Biochimie

authors： Vidal M,Sainte-Marie J,Philippot J,Bienvenue A

更新日期：1986-05-01 00:00:00

abstract：:Phospholipid analysis of the membranes associated with fast sedimenting folded chromosomes prepared by lysis of E. coli CR 34 shows that both inner and outer membranes are parts of the complex, in proportions not very different from that found in the whole bacteria. During the preparation of the folded chromosomes, th...

journal_title：Biochimie

authors： Lacombe C,Lubochinsky B

更新日期：1977-01-01 00:00:00