Abstract：
:Replication protein A (RPA) is a heterotrimeric single-stranded DNA binding protein whose role in DNA replication, recombination and repair has been mainly elucidated through in vitro biochemical studies utilizing the mammalian complex. However, the identification of homologs of all three subunits in Saccharomyces cerevisiae offers the opportunity of examining the in vivo role of RPA. In our laboratory, we have previously isolated a missense allele of the RFA1 gene, encoding the p70 subunit of the RPA complex. Strains containing this mutant allele, rfa1-D228Y, display increased levels of direct-repeat recombination, decreased levels of heteroallelic recombination, UV sensitivity and a S-phase delay. In this study, we have characterized further the role of RPA by screening other replication and repair mutants for a synthetic lethal phenotype in combination with the rfa1-D228Y allele. Among the replication mutants examined, only one displayed a synthetic lethal phenotype, pol12-100, a conditional allele of the B subunit of pol alpha-primase. In addition, a delayed senescence phenotype was observed in raf1-D228Y strains containing a null mutation of HDF1, the S. cerevisiae homolog of the 70 kDa subunit of Ku. Interestingly, a synergistic reduction in telomere length observed in the double mutants suggests that the shortening of telomeres may be the cause of the decreased viability in these strains. Furthermore, this result represents the first evidence of a role for RPA in telomere maintenance.
journal_name
Biochimiejournal_title
Biochimieauthors
Smith J,Zou H,Rothstein Rdoi
10.1016/s0300-9084(00)00183-8keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
71-8issue
1eissn
0300-9084issn
1638-6183pii
S0300-9084(00)00183-8journal_volume
82pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Concern about the malicious applications of botulinum neurotoxin has highlighted the need for a new generation of safe and highly potent antitoxins. In this study, we developed and evaluated the preclinical pharmacology and safety of a new F(ab')₂ antitoxin against botulinum neurotoxin serotype A (BoNT/A). As an alter...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.06.010
更新日期：2010-10-01 00:00:00
abstract：:The phosphotriesterase PTE, identified in the soil bacterium Pseudomonas diminuta, is thought to have evolved in the last several decades to degrade the pesticide paraoxon with proficiency approaching the limit of substrate diffusion (k(cat)/K(M) of 4 x 10(7)M(-1)s(-1)). It belongs to the amidohydrolase superfamily, b...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2007.01.007
更新日期：2007-05-01 00:00:00
abstract：:Influenza virus-associated RNA polymerase is composed of one molecule each of three viral P proteins and carries the complete activity of capped RNA-primed vRNA-directed transcription. The RNA polymerase holoenzyme also carries capped RNA endonuclease to generate capped oligonucleotide primers for transcription and 3'...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(97)86735-1
更新日期：1996-01-01 00:00:00
abstract：:When colonizing the digestive tract of mono-associated rats, Ruminococcus gnavus E1 - a bacterium isolated from human faeces - produced a trypsin-dependent anti-Clostridium perfringens substance collectively named Ruminococcin C (RumC). RumC was isolated from the caecal contents of E1-monocontaminated rats and found t...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.05.001
更新日期：2011-09-01 00:00:00
abstract：:During the suckling period, the rats are fed continuously with milk, which is a high-fat low-carbohydrate diet (HF). At weaning, milk is progressively replaced by the rat's laboratory chow which is a high-carbohydrate low-fat diet (HCHO), and this is accompanied by large hormonal modifications: an increase in plasma i...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(91)90077-e
更新日期：1991-01-01 00:00:00
abstract：:Cell cycle-related and expression elevated protein in tumor (CREPT) is emerging as a novel cancer-related gene that is dysregulated in many kinds of malignancies. However, the expression and biological role of CREPT in glioma remains unclear. In the present study, we aimed to explore the potential function and regulat...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.04.014
更新日期：2019-07-01 00:00:00
abstract：:The structure of the F0 part of ATP synthases from E. coli and Neurospora crassa was analyzed by hydrophobic surface labeling with [125I]TID. In the E. coli F0 all three subunits were freely accessible to the reagent, suggesting that these subunits are independently integrated in the membrane. Labeled amino acid resid...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(86)80010-4
更新日期：1986-03-01 00:00:00
abstract：:1)Individual monosaccharides (uronic acids and aminosugars) have been purified following specific hydrolysis of the mucopolysaccharides from new born rat skin (hyaluronic acid, heparin + heparan sulfate, chondroitin sulfate A, B and C), after incubation with [U14C]-glucose under various conditions and for varying incu...
journal_title：Biochimie
pub_type： 杂志文章
doi：
更新日期：1975-11-01 00:00:00
abstract：:A series of halomethylated derivatives of dihydrocoumarins has been found to inhibit irreversibly proteases and esterases. alpha-Chymotrypsin, subtilish, elastase are rapidly inactivated in the presence of these compounds, while trypsin, kallicrein, papain are inhibited more slowly. Esterases like acetylcholinesterase...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(77)80140-5
更新日期：1977-01-01 00:00:00
abstract：:The two subunits of the nickel-iron hydrogenase from Desulfovibrio gigas have been purified by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis and their amino acid compositions have been determined. The N-terminal sequences for 15 residues of the large subunit (Mr 62,000) and 25 residues of the s...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(88)90070-3
更新日期：1988-02-01 00:00:00
abstract：BACKGROUND:Accumulating evidence suggests that microRNA-590 (miR-590) has protective effects on cardiovascular diseases, but the mechanism is unknown. Interestingly, previous studies from our laboratory and others have shown that macrophage-derived lipoprotein lipase (LPL) might accelerate atherosclerosis by promoting ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.08.003
更新日期：2014-11-01 00:00:00
abstract：:Obesity prevalence continues to rise throughout the developed world, as a result of positive energy balance and reduced physical activity. At present, there is still a perception within the general community, and amongst some nutritionists, that eating multiple small meals spaced throughout the day is beneficial for w...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2015.07.025
更新日期：2016-05-01 00:00:00
abstract：:MicroRNAs (miRNAs) are ∼21 nt non-coding small RNAs which regulate gene expression at the post-transcriptional level in plants and animals. Until recently, only limited numbers of miRNAs were identified in Cucurbitaceae, a large flowering plant family. In this study, 220 potential miRNA candidates were identified from...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.03.002
更新日期：2014-07-01 00:00:00
abstract：:Ciguatoxin-like substances were extracted from the viscera or the flesh of eight Caribbean fish species, including small invertebrate feeders and large carnivores. The had similar properties, i.e. pharmacological action, solubility, chromatographic behaviour on silicic acid or Sephadex LH 20 column, stability in a wea...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(86)80025-6
更新日期：1986-03-01 00:00:00
abstract：:Human Rad51 (HsRad51), a key element of the homologous recombination repair pathway, is related to the resistance of cancer cells to chemo- and radio-therapies. This protein is thus a good target for the development of anti-cancer treatments. We have searched for new inhibitors directed against HsRad51 using the Syste...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.08.006
更新日期：2010-12-01 00:00:00
abstract：:The reactivity of sonicated phosphatidylcholine-cholesterol liposomes with cholesterol : oxygene oxydoreductase, an enzyme which catalyses the oxidation of the 3 beta hydroxyl group of cholesterol to a ketone group, is compared with that of ternary system phosphatidylcholine-cholesterol-Thesit. Regardless to the phosp...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(79)80205-9
更新日期：1979-01-01 00:00:00
abstract：:Administration of beta-adrenergic agonists to domestic species can lead to skeletal muscle hypertrophy, probably by reducing the rate of myofibrillar protein breakdown. Myofibrillar breakdown is associated with the calcium-dependent proteinase system (calpains I,II and calpastatin) whose activity also changes during b...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(92)90125-x
更新日期：1992-03-01 00:00:00
abstract：:The hepatitis E virus (HEV) is the most common cause of acute hepatitis worldwide. Although HEV is a small, naked RNA virus, HEV particles become associated with lipids in the blood of infected patients and in the supernatant of culture systems. The egress of these particles from cells implies the exocytosis pathway b...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.05.003
更新日期：2017-10-01 00:00:00
abstract：:Pseudomonas cepacia 122 can grow on several lignin model compounds including the arylglycerol-beta-aryl ethers guaiacylglycerol-beta-coniferyl ether and guaiacylglycerol-beta-guaiacyl ether. Non-phenolic lignin model compounds are not degraded by this bacterium. The enzyme system catalyzing guaiacylglycerol-beta-guaia...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80047-x
更新日期：1985-02-01 00:00:00
abstract：:Recently we have found that cytokine IL-2 and innate immunity protein Tag7 activate cytotoxic lymphocytes that kill HLA-negative tumor cells, inducing both apoptosis and necroptosis. Here we decrypt the processes, taking part in necroptosis execution after FasL-Fas interaction. Necroptosis begins with RIPK1 activation...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2018.07.003
更新日期：2018-09-01 00:00:00
abstract：:In septic shock patients, alterations of plasma phospholipid fatty acid profile have never been described. The purpose of this monocentric, non-interventional, observational prospective study was to describe this fatty acid profile in the early phase of septic shock in intensive care unit. Thirty-seven adult patients ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.08.006
更新日期：2013-11-01 00:00:00
abstract：:Interleukin-17 (IL-17) is produced exclusively by activated T cells and neutrophils, and stimulates osteoclastic bone resorption via osteoblasts by inducing the expression of "receptor activator of NF-kappaB (RANK) ligand" (RANKL). However, the direct effects of IL-17 on the differentiation of osteoclast precursors in...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2009.12.011
更新日期：2010-04-01 00:00:00
abstract：:Ligation of CD47 by its natural ligand thrombospondin (TSP), or cross-linking by CD47 antibodies, triggers caspase-independent cell death in normal and leukemic cells. This kind of cell death is characterised by the cytoplasmic events of apoptosis including externalisation of phosphatidylserines and mitochondria swell...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(03)00129-9
更新日期：2003-08-01 00:00:00
abstract：:A variety of species of GPDH undergo acylation at two of the four active cystein sites per mole of tetrameric enzyme. This reaction requires tightly bound NAD+, a situation restricted to two of the four NAD sites per tetramer. S leads to N acyl transfer from cysteins to lysine in the diacyl enzyme yields an inactive e...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(81)80177-0
更新日期：1981-02-01 00:00:00
abstract：:A variety of transcription attenuation mechanisms are used by bacteria to regulate gene and operon expression. This review summarizes previous and current studies designed to elucidate the features of the specific attenuation mechanisms that regulate expression of the tryptophanase (tna) operon of Escherichia coli and...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/s0300-9084(97)86725-9
更新日期：1996-01-01 00:00:00
abstract：:Peroxisomal ABC transporters encoded by the ABCD genes are thought to participate in the import of specific fatty acids in the peroxisomal matrix. ABCD1 deficiency is associated with X-linked adrenoleukodystrophy (X-ALD), the most frequent peroxisomal disorder which is characterized by the accumulation of saturated ve...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2008.05.022
更新日期：2008-10-01 00:00:00
abstract：:Recent work has shown that a DBF4 analog in yeast may be a target of nitrogen-containing bisphosphonates. DBF4 is an essential protein kinase required for DNA replication from primary eukaryotes to humans and appears to play a critical role in the S-phase checkpoint. It is also required for cell migration and cell sur...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.11.010
更新日期：2014-04-01 00:00:00
abstract：:In order to identify conserved potential secondary structures within translational start sites, mRNA sequences derived from different species were studied with programs able to depict such features. The potential secondary structure of 71 bases around the initiator AUG or AUGs in the coding sequences of 290 eukaryotic...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(94)90120-1
更新日期：1994-01-01 00:00:00
abstract：:The oncogenic potential of the high-risk human papillomavirus (HPV) relies on the expression of genes specifying the E7 and E6 proteins. To investigate further the variation in oligomeric structure that has been reported for different E7 proteins, an HPV-18 E7 cloned from a Hispanic woman with cervical intraepithelial...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.02.028
更新日期：2010-07-01 00:00:00
abstract：:This paper provides an overview of the usefulness of the resonance Raman (RR) spectroscopy in the determination of the structural and electronic properties of heme(s) included in c-type cytochromes. It reviews the mode assignments presently available for heme c and includes recent RR data on the most important subclas...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(94)90145-7
更新日期：1994-01-01 00:00:00
