Abstract：
:1)Individual monosaccharides (uronic acids and aminosugars) have been purified following specific hydrolysis of the mucopolysaccharides from new born rat skin (hyaluronic acid, heparin + heparan sulfate, chondroitin sulfate A, B and C), after incubation with [U14C]-glucose under various conditions and for varying incubation periods. The yields and the specificity of the methods used for hydrolysis are discussed. 2) Monosaccharides from hyaluronic acid and the sulfated mucopolysaccharide fraction are labelled at an approximately equal rate. In addition, high rates of labelling of glucosamine isolated from the sulfated fractions confirms the preferential labelling of (heparin + heparan sulfate) demonstrated with the sulfated polymers. 3) In all fractions, aminosugars are considerably less labelled than the corresponding uronic acids, which suggests the existence of endogeneous diluting precursor pools for the former monosaccharides. 4) No drift of radioactivity from D-glucuronate to L-iduronate could be demonstrated in sulfated mucopolysaccharides after inhibition of their biosynthesis by puromycin or diluting the labelled precursor pools. Hence it has not been possible to substantiate on the surviving tissue the C5 epimerization at the polymer level, as previously demonstrated by other authors with subcellular fractions of various origin.
journal_name
Biochimiejournal_title
Biochimieauthors
Hervé B,Clauser Hkeywords:
subject
Has Abstractpub_date
1975-11-01 00:00:00pages
1341-5issue
11-12eissn
0300-9084issn
1638-6183journal_volume
57pub_type
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