Abstract:
:Protein l-isoaspartyl-O-methyltransferase (PIMT) is an ubiquitous enzyme widely distributed in cells and plays a role in the repair of deamidated and isomerized proteins. In this study, we show that this enzyme is present in cytosolic extract of Vibrio cholerae, an enteric pathogenic Gram-negative bacterium and is enzymatically active. Additionally, we focus on the detailed biophysical characterization of the recombinant PIMT from V. cholerae to gain insight into its structure, stability and the cofactor binding. The equilibrium denaturation of PIMT has been studied using tryptophan fluorescence and CD spectroscopy. The far- and near-UV CD, as well as fluorescence experiments reveal the presence of a non-native intermediate in the folding pathway. Binding of the hydrophobic fluorescent probe, bis-ANS, to the intermediate occurs with high affinity because of the exposure of the hydrophobic clusters during the unfolding process. The existence of the probable intermediate has also been confirmed from limited tryptic digestion and DLS experiments. The protein shows higher binding affinity for AdoHcy, in comparison to AdoMet, and the binding increases the midpoint of thermal unfolding by 6 and 5 °C, respectively. Modeling and molecular dynamics simulations also support the higher stability of the protein in presence of AdoHcy.
journal_name
Biochimiejournal_title
Biochimieauthors
Chatterjee T,Pal A,Chakravarty D,Dey S,Saha RP,Chakrabarti Pdoi
10.1016/j.biochi.2012.12.013subject
Has Abstractpub_date
2013-04-01 00:00:00pages
912-21issue
4eissn
0300-9084issn
1638-6183pii
S0300-9084(12)00504-4journal_volume
95pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Diacylglycerol kinase (DGK) phosphorylates diacylglycerol to produce phosphatidic acid (PA). The η isozyme of DGK is abundantly expressed in C2C12 myoblasts. However, the role of DGKη in skeletal muscle cells remains unknown. In the present study, we showed that DGKη was downregulated at an early stage of myogenic dif...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.07.018
更新日期:2020-10-01 00:00:00
abstract::The cartilage tissue has a limited self-regenerative capacity. Tissue-engineering represents a promising trend for cartilage repair. The present study was aimed to develop a biomaterial formulation by combining fragments of chitosan hydrogel with isolated rabbit or human chondrocytes. We first reported the properties ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2006.03.002
更新日期:2006-05-01 00:00:00
abstract::An 18.2 kDa protein from the liver fluke, Fasciola hepatica has been identified and characterised. The protein shows strongest sequence similarity to egg antigen proteins from Schistosoma mansoni, Schistosoma japonicum and Clonorchis sinensis. The protein is predicted to adopt a calmodulin-like fold; it thus represent...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.06.015
更新日期:2012-11-01 00:00:00
abstract::I have been involved in research on oxysterols since 1963 and this review is intended to cover some of the most important aspects of this work. The first project dealed with 7α-hydroxy-4-cholesten-3-one. My successful synthesis of this steroid with high specific radioactivity allowed a demonstration that it is a bile ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2012.02.029
更新日期:2013-03-01 00:00:00
abstract::Streptomyces sp. 139 produces a novel exopolysaccharide (EPS) designated Ebosin which can bind IL-1R specifically and exhibits anti-rheumatic arthritis activity in vivo. With the Ebosin biosynthesis gene cluster (ste) consisting of 27 ORFs identified previously the focus of this study was to characterize the protein e...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.04.014
更新日期:2011-09-01 00:00:00
abstract::Simian Virus 40 (SV40) DNA replication was studied in vitro using cell free extracts prepared from SV40 infected CV1 cells. The cells were fractionated into a soluble cytoplasmic fraction and nuclei. The nuclei were lysed with high salt and used to prepare a soluble nuclear fraction. Both fractions displayed DNA polym...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80088-0
更新日期:1976-11-13 00:00:00
abstract::The genus Populus is an effective model in tree genetics. This genus includes dioecious species and, recently, whole genome resequencing of P. trichocarpa and P. balsamifera enabled the identification of sex-linked regions and sex-associated single nucleotide polymorphisms (SNPs). These results created new opportuniti...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.03.018
更新日期:2019-07-01 00:00:00
abstract::Caveolin is the protein marker of caveola-mediated endocytosis. Previously, we demonstrated by immunoblotting and immunofluorescence that an anti-chick embryo caveolin-1 monoclonal antibody (mAb) recognizes a protein in amoeba extracts. Nevertheless, the caveolin-1 gene is absent in the Entamoeba histolytica genome da...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.09.007
更新日期:2014-12-01 00:00:00
abstract::Phospholipases A2 (PLA2) are a family of enzymes that catalyze the hydrolysis of the sn-2 ester bond of glycerophospholipids liberating lysophospholipids and free fatty acids; important second messengers involved in atherogenesis. Plasma PAF-acetylhydrolase (PAF-AH) or Lp-PLA2 is a Ca(2+)-independent PLA2 which is pro...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2010.02.002
更新日期:2010-06-01 00:00:00
abstract::The hydrolysis of trioctanoin and of monoolein by rat intestinal homogenates, catalyzed by the previously described "trioctanoin hydrolase" and "monoglyceride lipase" [Ref. 6 and 11], was shown to be entirely due to the action of the intestinal glycerol-ester hydrolase [Ref. 1 and 2] and to contaminating pancreatic li...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(77)80320-9
更新日期:1977-01-01 00:00:00
abstract::The development of in vitro repair assays with human cell-free extracts led to new insights on the mechanism of excision of DNA damage which consists of incision/excision and repair synthesis/ligation. We have adapted the repair synthesis reaction with cells extracts incubated with damaged plasmid DNA performed in liq...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(99)80038-8
更新日期:1999-01-01 00:00:00
abstract::Peroxisomal ABC transporters encoded by the ABCD genes are thought to participate in the import of specific fatty acids in the peroxisomal matrix. ABCD1 deficiency is associated with X-linked adrenoleukodystrophy (X-ALD), the most frequent peroxisomal disorder which is characterized by the accumulation of saturated ve...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2008.05.022
更新日期:2008-10-01 00:00:00
abstract::When colonizing the digestive tract of mono-associated rats, Ruminococcus gnavus E1 - a bacterium isolated from human faeces - produced a trypsin-dependent anti-Clostridium perfringens substance collectively named Ruminococcin C (RumC). RumC was isolated from the caecal contents of E1-monocontaminated rats and found t...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2011.05.001
更新日期:2011-09-01 00:00:00
abstract::Kinetic parameters characterizing the catalytic activities of enzymes are typically investigated in dilute solutions. However, in reality, these reactions occur in cells that, in addition to water and ions, are full of other macromolecules including proteins, nucleic acids, lipids, and metabolites. Such a crowded envi...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.07.009
更新日期:2020-09-01 00:00:00
abstract::Protein-induced DNA bridging and looping is a common mechanism for various and essential processes in bacterial chromosomes. This mechanism is preserved despite the very different bacterial conditions and their expected influence on the thermodynamic and kinetic characteristics of the bridge formation and stability. O...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2017.08.002
更新日期:2017-11-01 00:00:00
abstract::In 1998, the concept of "green chemistry" was established through twelve principles with the aim of improving the eco-efficiency of chemical processes and to judge, whether or not, a chemical process is sustainable. Currently, numerous processes do not obey to most of these principles (large energy usage, formation of...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2015.09.019
更新日期:2016-01-01 00:00:00
abstract::Estrogen plays an essential role in bone formation, and estrogen modulation dysfunction is tightly associated with postmenopausal osteoporosis (PMOP). The underlying mechanisms of estrogen-mediated osteogenic differentiation have not been well defined. In this study, murine bone marrow mesenchymal stem cells were indu...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.01.001
更新日期:2015-03-01 00:00:00
abstract::The effect of adenosine was tested on the energetic metabolism of fed rat liver cells after isolation. The cells were incubated in a buffered saline medium with glucose (5 mM) and adenosine (1 mM) for 30 minutes at 37 degrees C. This increased the concentration of the adenylic nucleotides ATP (+57 per cent, ADP (+39 p...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(80)80286-0
更新日期:1979-01-01 00:00:00
abstract::The toxicity of amyloids is a subject under intense scrutiny. Many studies link this toxicity to the existence of various intermediate structures prior to the fiber formation and/or their specific interaction with membranes. Membranes can also be a catalyst of amyloidogenesis and the composition or the charge of membr...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2012.07.011
更新日期:2013-01-01 00:00:00
abstract::The field of immunometabolism has come a long way in the past decade, leading to the emergence of a new role for white adipose tissue (WAT) that is now recognized to stand at the junction of immune and metabolic regulations. Interestingly, a crucial role of the abundant and heterogeneous immune population present in W...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2015.06.012
更新日期:2016-05-01 00:00:00
abstract::Since discovery of high-temperature superconductive (HTS) ceramics by Bednorz and Muller in 1986, there has been an accelerated development of cold technologies in industry, including the domain of NMR detection. The purpose of this paper is to fix ideas about the stage that cryogenic radio frequency (RF) probe techni...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2003.09.016
更新日期:2003-09-01 00:00:00
abstract::Glycogen was described as a temporal storage molecule in rhodococci, interconnecting lipids and carbon availability. The Rhodococcus jostii ADP-glucose pyrophosphorylase (ADP-GlcPPase) kinetic and regulatory properties support this role. Curiously, the enzyme uses glucosamine-1P as alternative substrate. Herein, we re...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2020.07.006
更新日期:2020-09-01 00:00:00
abstract::Over the last couple of decades, the PPAR family of transcription factors has received much attention from the pharmaceutical industry due to their profound ability to improve glucose and lipid metabolism upon agonist activation. However, more recently the interest in these nuclear receptors has faded because several ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2016.10.017
更新日期:2017-05-01 00:00:00
abstract::Porcine pancreatic extracts (PPE), also named pancreatin, are commonly used as a global source of pancreatic enzymes for enzyme replacement therapy in patients with exocrine pancreatic insufficiency. They are considered as a good substitute of human pancreatic enzymes and they have become a material of choice for in v...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2019.07.004
更新日期:2020-02-01 00:00:00
abstract::The bovine oxytocin precursor was expressed in Escherichia coli as a fusion protein by cloning the hormone encoding cDNA in frame behind the replicase gene of the RNA phage MS2. By step-wise extraction with different urea concentrations, the fusion protein was enriched in the 7 M urea fraction and further purified by ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90165-4
更新日期:1988-01-01 00:00:00
abstract::Our previous researches showed that nitronyl nitroxyl derivatives, NNP and NNVP were good anti-oxidants and provided radioprotective effects in C6 cells. The objective of the present study is to investigate the possible antiviral effects and underlying pharmacological of the two nitronyl nitroxide radicals against CVB...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.05.015
更新日期:2012-09-01 00:00:00
abstract::A nuclear poly(ADP-ribose) polymerase (PARP) is activated by gamma-irradiation and consequently synthesizes poly(ADP-ribose) by binding to DNA strand-breaks. This property suggests that PARP is a DNA strand-break-signal generator. Meanwhile, the cell-cycle arrest occurs in G1 and G2 phases following gamma-irradiation....
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(96)88161-2
更新日期:1995-01-01 00:00:00
abstract::In the process of L-phenylalanine oxidation by Proteus mirabilis cytoplasmic membrane, hydrogen peroxide was produced at a rate corresponding to 1-3 per cent of the total electron flow (30-110 nmoles min-1mg-1). Peroxide was estimated using a fluorimetric assay with horseradish peroxidase, or by anodic oxidation on a ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(82)80351-9
更新日期:1982-10-01 00:00:00
abstract::SR-PSOX can function as a scavenger receptor, a chemokine and an adhesion molecule, and it could be an interesting player in the formation of atherosclerotic lesions. Our previous studies demonstrated that basic amino acid residues in the chemokine domain of SR-PSOX are critical for its functions. In this study the co...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.09.016
更新日期:2013-02-01 00:00:00
abstract::Excitation-contraction coupling describes the series of events that beginning with propagated action potential on the muscle fiber surface membrane, leads to the twitch contraction of the fiber. Myotonic disorders are inherited diseases whose clinical manifestations include electrophysiological signs such as increased...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(87)90032-0
更新日期:1987-04-01 00:00:00