Expression of the bovine hypothalamic hormone oxytocin precursor in Escherichia coli.

abstract：:The present review deals with the place of single chain oligonucleotide ligands (aptamers) in affinity chromatography applied to proteins. Aptamers are not the only affinity ligands available but they represent an emerging and highly promising route that advantageously competes with antibodies in immunopurification pr...

journal_title：Biochimie

authors： Perret G,Boschetti E

更新日期：2018-02-01 00:00:00

abstract：:We have synthesized and examined a number of fully and partly self-complementary palindromic oligodeoxynucleotides for their ability to assume in solution a unimolecular hairpin structure. The main results obtained by a combined optical and electrophoresis investigation show that: (i) DNA folding needs not be driven b...

journal_title：Biochimie

authors： Xodo LE,Manzini G,Quadrifoglio F,van der Marel G,van Boom JH

更新日期：1989-07-01 00:00:00

abstract：:Tri(n-butyl)phosphate (TNBP) and sodium cholate (SC) mixtures have been used to inactivate lipid-enveloped viruses like HIV and hepatitis B. We exploited the use of this combination to purify fibroblast growth factor-2 (FGF-2) from human placenta. Human placentas were extracted in the presence of 0.3% TNBP/0.2% SC and...

journal_title：Biochimie

authors： Wolff P,Tanaka AM,Chenker E,Cabrera-Crespo J,Raw I,Ho PL

更新日期：1996-01-01 00:00:00

abstract：:The effects of various concentrations of 2-fluoro-2-deoxy-D-glucose (FDG) on the aerobic metabolism of glucose and the reciprocal effect of glucose on the metabolism of FDG in glucose-grown repressed Saccharomyces cerevisiae cells were studied at 30 degrees C in a standard pyrophosphate medium containing 5 x 10(7) cel...

journal_title：Biochimie

authors： Tran-Dinh S,Courtois A,Wietzerbin J,Bouet F,Herve M

更新日期：1995-01-01 00:00:00

abstract：:Coenzymes are small organic molecules that supply a varied set of reactive groups to protein enzymes, thereby diversifying catalysis beyond the chemistries of amino acid sidechains. As RNA structures begin with a more limited chemical diversity than proteins, it seems likely that RNA enzymes would also use functional ...

journal_title：Biochimie

authors： Jadhav VR,Yarus M

更新日期：2002-09-01 00:00:00

abstract：:Histone acetyltransferase (Hpa2) is an unusual acetyltransferase, with a wide range of substrates; including histones, polyamines and aminoglycosides antibiotic. Hpa2 belongs to GNAT superfamily and GNATs are well known for the formation of homo-oligomers. However, the reason behind their oligomerization remained unex...

journal_title：Biochimie

authors： Tomar JS,Hosur RV

更新日期：2020-01-01 00:00:00

abstract：:gamma-Aminobutyraldehyde dehydrogenase from Escherichia coli K-12 has been purified and characterized from cell mutants able to grow in putrescine as the sole carbon and nitrogen source. The enzyme has an Mr of 195,000 +/- 10,000 in its dimeric form with an Mr of 95,000 +/- 1,000 for each subunit, a pH optimum at 5.4 ...

journal_title：Biochimie

authors： Prieto MI,Martin J,Balaña-Fouce R,Garrido-Pertierra A

更新日期：1987-11-01 00:00:00

abstract：:Thyroglobulin is a large-size iodoglycoprotein specific to thyroid tissue and is the substrate for the synthesis of thyroid hormones, thyroxine and 3,5,3'-triiodothyronine. Recent studies, which greatly benefited from recombinant DNA methodologies, improved the knowledge of several structural features of this dimeric ...

journal_title：Biochimie

authors： Malthièry Y,Marriq C,Bergé-Lefranc JL,Franc JL,Henry M,Lejeune PJ,Ruf J,Lissitzky S

更新日期：1989-02-01 00:00:00

abstract：:Cell walls of flocculent strains (0006) and non flocculent strains (0019) of Saccharomyces uvarum (Carlsbergensis), grown in different media and taken in both growth and stationary phases, were treated with water and with 2 per cent (W/V) potassium hydroxide. This treatment yielded four fractions (FI, FII, FIII and FR...

journal_title：Biochimie

authors： Amri MA,Bonaly R,Duteurtre B,Moll M

更新日期：1981-07-01 00:00:00

abstract：:Protein l-isoaspartyl-O-methyltransferase (PIMT) is an ubiquitous enzyme widely distributed in cells and plays a role in the repair of deamidated and isomerized proteins. In this study, we show that this enzyme is present in cytosolic extract of Vibrio cholerae, an enteric pathogenic Gram-negative bacterium and is enz...

journal_title：Biochimie

authors： Chatterjee T,Pal A,Chakravarty D,Dey S,Saha RP,Chakrabarti P

更新日期：2013-04-01 00:00:00

abstract：:Nitrogen fixation in A. vinelandii and R. leguminosarum bacteroides shows identical characteristics with respect to the dependence on membrane energization, the sensitivity to uncouplers, the ATP/ADP-ratio, and the dependences on flavodoxinhydroquinone as electrondonor. Although we have been successful in preparing in...

journal_title：Biochimie

authors： Veeger C,Laane C,Scherings G,van Zeeland Wolbers L

更新日期：1978-01-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) and αvβ3 integrin are key molecules that actively participate in tumor angiogenesis and metastasis. Some integrin-blocking molecules are currently under clinical trials for cancer and metastasis treatment. However, the mechanism of action of such inhibitors is not completely u...

journal_title：Biochimie

authors： Montenegro CF,Salla-Pontes CL,Ribeiro JU,Machado AZ,Ramos RF,Figueiredo CC,Morandi V,Selistre-de-Araujo HS

更新日期：2012-08-01 00:00:00

abstract：:Obesity prevalence continues to rise throughout the developed world, as a result of positive energy balance and reduced physical activity. At present, there is still a perception within the general community, and amongst some nutritionists, that eating multiple small meals spaced throughout the day is beneficial for w...

journal_title：Biochimie

authors： Hutchison AT,Heilbronn LK

更新日期：2016-05-01 00:00:00

abstract：:Human mitochondrial aminoacyl-tRNA synthetases (mt-aaRSs), the enzymes which esterify tRNAs with the cognate specific amino acid, form mainly a different set of proteins than those involved in the cytosolic translation machinery. Many of the mt-aaRSs are of bacterial-type in regard of sequence and modular structural o...

journal_title：Biochimie

authors： Messmer M,Blais SP,Balg C,Chênevert R,Grenier L,Lagüe P,Sauter C,Sissler M,Giegé R,Lapointe J,Florentz C

更新日期：2009-05-01 00:00:00

abstract：:Gastrokine1 (GKN1), important for maintaining the physiological function of the gastric mucosa, is highly expressed in the stomach of healthy individuals but is down-regulated or absent in gastric tumor tissues and derived cell lines. The mechanisms underlying GKN1 gene inactivation are still unknown. We previously sh...

journal_title：Biochimie

authors： Stella di Stadio C,Faraonio R,Federico A,Altieri F,Rippa E,Arcari P

更新日期：2019-12-01 00:00:00

abstract：:The short isoform of Rho guanine nucleotide exchange factor ARHGEF5 is known as TIM, which plays diverse roles in, for example, tumorigenesis, neuronal development and Src-induced podosome formation through the activation of its substrates, the Rho family of GTPases. The activation is auto-inhibited by a putative heli...

journal_title：Biochimie

authors： He P,Tan DL,Liu HX,Lv FL,Wu W

更新日期：2015-04-01 00:00:00

abstract：:The eukaryotic ribosomal protein S15 is a key component of the decoding site in contrast to its prokaryotic counterpart, S19p, which is located away from the mRNA binding track on the ribosome. Here, we determined the oligopeptide of S15 neighboring the A site mRNA codon on the human 80S ribosome with the use of mRNA ...

journal_title：Biochimie

authors： Khairulina J,Graifer D,Bulygin K,Ven'yaminova A,Frolova L,Karpova G

更新日期：2010-07-01 00:00:00

abstract：:The nuclear metabolism of poly(ADP-ribose) is mainly regulated by poly(ADP-ribose) polymerase-1 (PARP-1) and by poly(ADP-ribose) glycohydrolase (PARG). A PARP-like enzyme, V-PARP, and a PARG isoform are present in the extra-nuclear compartment of mammalian cells, even if poly(ADP-ribose) has never been detected therei...

journal_title：Biochimie

authors： Rossi L,Denegri M,Torti M,Poirier GG,Ivana Scovassi A

更新日期：2002-12-01 00:00:00

abstract：:Bothropstoxin-I (BthTx-I) is a homodimeric Lys49-PLA2 from the venom of the snake Bothrops jararacussu, which lacks hydrolytic activity against phospholipid substrates, yet permeabilizes membranes by a Ca2+-independent mechanism. The interaction of the BthTx-I with model membranes has been studied by intrinsic tryptop...

journal_title：Biochimie

authors： Ferreira TL,Ruller R,Chioato L,Ward RJ

更新日期：2008-09-01 00:00:00

abstract：:The Nicotiana tabacum Nt-4/1 protein is a plant-specific protein of unknown function. Analysis of bacterially expressed Nt-4/1 protein in vitro revealed that the protein secondary structure is mostly alpha-helical and suggested that it could consist of three structural domains. Earlier studies of At-4/1, the Arabidops...

journal_title：Biochimie

authors： Solovyev AG,Minina EA,Makarova SS,Erokhina TN,Makarov VV,Kaplan IB,Kopertekh L,Schiemann J,Richert-Pöggeler KR,Morozov SY

更新日期：2013-07-01 00:00:00

abstract：:Halophilic proteins have evolved to be soluble, stable and active in high salt concentration. Crystallographic studies have shown that surface enrichment by acidic amino acids is a common structural feature of halophilic proteins. In addition, ion-binding sites have also been observed in most of the cases. The role of...

journal_title：Biochimie

authors： Madern D,Ebel C

更新日期：2007-08-01 00:00:00

abstract：:We have purified, by a three step procedure, a yeast C-nitrosoreductase and studied its action on p-nitroso-N,N-dimethylaniline. Microcalorimetric measurements performed on the microcalorimeter LKB "batch", have been given the number of protons exchanged during the reaction : four protons are involved. Two are given b...

journal_title：Biochimie

authors： Huynh CH

更新日期：1975-01-01 00:00:00

abstract：:We have previously proposed that the rate of ribosome function during balanced growth in E. coli, expressed as the rate of peptide chain elongation, is adjusted by a feedback mechanism: whenever that rate is submaximal (i.e. below 22 amino acid residues polymerized per active ribosome at 37 degrees C), the feedback si...

journal_title：Biochimie

authors： Bremer H,Dennis P

更新日期：2008-03-01 00:00:00

abstract：:The combination of cDNA and RNA sequencing techniques has enabled determination of the complete sequence of one of the mRNAs coding for the precursor of the small subunit of ribulose bisphosphate carboxylase of Nicotiana sylvestris. In this 898-nucleotide-long mRNA, 540 nucleotides code for the entire 180-amino-acid-l...

journal_title：Biochimie

authors： Pinck M,Guilley E,Durr A,Hoff M,Pinck L,Fleck J

更新日期：1984-07-01 00:00:00

abstract：:A novel approach to genetic sequence analysis is presented. This approach, based on compression of algorithms, has been launched simultaneously by Grumbach and Tahi, Milosavljevic and Rivals. To reduce the description of an object, a compression algorithm replaces some regularities in the description by special codes....

journal_title：Biochimie

authors： Rivals E,Dauchet M,Delahaye JP,Delgrange O

更新日期：1996-01-01 00:00:00

abstract：:The methylotrophic yeast Pichia pastoris is often used as an organism for the heterologous expression of proteins and has been used already for production of a number of glycosyltransferases involved in the biosynthesis of N- and O-linked oligosaccharides. In our recent studies, we have examined the expression in P. p...

journal_title：Biochimie

authors： Bencúrová M,Rendić D,Fabini G,Kopecky EM,Altmann F,Wilson IB

更新日期：2003-03-01 00:00:00

abstract：:The mouse interferon alpha 7 gene, the signal sequence of which has been removed by oligonucleotide-directed mutagenesis, was introduced into a Bacillus subtilis secretion vector containing the promoter and the signal sequence of the B. subtilis levansucrase gene. Different B. subtilis strains were transformed with th...

journal_title：Biochimie

authors： Dion M,Rapoport G,Doly J

更新日期：1989-06-01 00:00:00

abstract：:Leptin is a cytokine secreted by the adipose tissue that is involved in the control of body weight. We previously showed that a point mutation (R105W) in leptin results in leptin deficiency, marked obesity and hypogonadism in humans adults. Expression in COS1 cells showed impaired secretion and intracellular accumulat...

journal_title：Biochimie

authors： Boute N,Zilberfarb V,Camoin L,Bonnafous S,Le Marchand-Brustel Y,Issad T

更新日期：2004-06-01 00:00:00

abstract：:The structural difference of flavonoids strongly affects the binding process with plasma proteins. This work in here mainly concerns about the molecular property-binding affinity relationship of dietary flavonoids for common rat plasma proteins (CRPP). The magnitudes of binding constants between flavonoids and CRPP we...

journal_title：Biochimie

authors： Xiao J,Cao H,Chen T,Yang F,Liu C,Xu X

更新日期：2011-02-01 00:00:00

abstract：:The imprinted gene Grb10 influences fetal growth, adult behavior and tumor formation. Investigating this gene often involves consecutive passaging of cells in vitro. We analyzed changes in Grb10 DNA methylation and histone modifications in serially passaged adult mouse tail-tip fibroblast cells with bisulfite genomic ...

journal_title：Biochimie

authors： Yao J,Huang Y,Huang R,Shi R,Chen P,Zhu B,Li M,Jiang X,Zheng M,Jiang Y,Yang X

更新日期：2012-12-01 00:00:00