Thyroglobulin structure and function: recent advances.

abstract：:Pleurocidin (GWGSFFKKAAHVGKHVGKAALTHYL-NH(2)), found in skin mucous secretions of the winter flounder Pleuronectes americanus, is known to possess a high potency and broad-spectrum antimicrobial peptide without cytotoxicity. In this study, to investigate the impact of pleurocidin on apoptotic progress, we observed mor...

journal_title：Biochimie

authors： Cho J,Lee DG

更新日期：2011-10-01 00:00:00

abstract：:RNA-protein crosslinks were introduced into the 40S ribosomal subunits from Saccharomyces cerevisiae by mild UV treatment. Proteins crosslinked to the 18S rRNA molecule were separated from free proteins by repeated extraction of the treated subunits and centrifugation in glycerol gradients. After digestion with RNase ...

journal_title：Biochimie

authors： Xiang RH,Lee JC

更新日期：1989-11-01 00:00:00

abstract：:The interactions between lactic acid bacteria and their phages are commercially significant. Current research has focused on the elucidation of the mechanisms and genetics of phage resistance. Phage resistance genes have been linked to plasmid DNA for Streptococcus lactis and Streptococcus cremoris, and preliminary st...

journal_title：Biochimie

authors： Sanders ME

更新日期：1988-03-01 00:00:00

abstract：:Sialic acid, a nine-carbon sugar acid usually is present in the non-reducing terminal position of free oligosaccharides and glycoconjugates. Sialylated conjugates in mammals perform important roles in cellular recognition, signaling, host-pathogen interaction and neuronal development. Metabolism of sialylated conjugat...

journal_title：Biochimie

authors： Wickramasinghe S,Medrano JF

更新日期：2011-10-01 00:00:00

abstract：:Murine L5178Y cell ribosomes were dissociated into subunits either with potassium chloride in the presence of puromycin or with the chelating agent EDTA. The proteins of ribosomal subunits obtained by these different methods were compared by means of bidimensional polyacrylamide gel electrophoresis. KCl-derived 60S an...

journal_title：Biochimie

authors： Longuet M,Auger-Buendia MA,Tavitian A

更新日期：1979-01-01 00:00:00

abstract：:Crystals of flavoenzyme L-lactate oxidase from Aerococcus viridans (LOX) have been obtained that diffract to 3.0 A resolution (P2(1)2(1)2(1), a = 118.4 A, b = 138.4 A, c = 194.6 A). Crystallographic studies suggest that the enzyme may exist as an octameric form with non-crystallographic two- and four-fold axes in the ...

journal_title：Biochimie

authors： Morimoto Y,Yorita K,Aki K,Misaki H,Massey V

更新日期：1998-04-01 00:00:00

abstract：:The kinetics of the native glycogen phosphorylase b from rabbit skeletal muscle and of the enzyme specifically deiminated by peptidylarginine deiminase have been studied. One arginine residue per phosphorylase b monomer is transformed into citrulline after 3 h of incubation with peptidylarginine deiminase. The maximal...

journal_title：Biochimie

authors： Eronina TB,Livanova NB,Chebotareva NA,Kurganov BI,Luo S,Graves DJ

更新日期：1996-01-01 00:00:00

abstract：:Lactic acid bacteria provide a model system for the in vivo study of mechanisms pertaining to the regulation of sugar transport and metabolism by microorganisms. Recent studies with resting and growing cells of the homofermentative Streptococci and Lactobacilli have yielded evidence for hitherto unsuspected regulatory...

journal_title：Biochimie

authors： Thompson J

更新日期：1988-03-01 00:00:00

abstract：:Here we describe a convenient and robust ligase-independent method for construction of combinatorial and random mutant libraries. The homologous genes flanked by plasmid-derived DNA sequences are fragmented, and the random fragments are reassembled in a self-priming polymerase reaction to obtain chimeric genes. The pr...

journal_title：Biochimie

authors： An Y,Wu W,Lv A

更新日期：2010-08-01 00:00:00

abstract：:The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffii is reported. The structure was solved by MIRAS procedure and refined to a crystallographic residual of 17.3% (R(free) = 20.3%) at 1.8 A resolution. Two isomorphous derivatives were obtained by quick cryo-soaking approach. CTI...

journal_title：Biochimie

authors： Krauchenco S,Nagem RA,da Silva JA,Marangoni S,Polikarpov I

更新日期：2004-03-01 00:00:00

abstract：:Versican and decorin, two proteoglycans (PGs) with contradictory roles in the pathophysiology of cancer, comprise important stromal components in many tumor types and play a crucial role in the progression of cancer. In this study, we provide direct evidence for a significant and stage-related accumulation of versican...

journal_title：Biochimie

authors： Skandalis SS,Theocharis AD,Papageorgakopoulou N,Vynios DH,Theocharis DA

更新日期：2006-09-01 00:00:00

abstract：:NikR proteins are bacterial metallo-regulatory transcription factors that control the expression of the nickel uptake system and/or nickel containing enzymes such as urease, and are involved in the acid stress response. Here, a comparative study is reported on NikR from Helicobacter pylori (HpNikR) and Escherichia col...

journal_title：Biochimie

authors： Fauquant C,Diederix RE,Rodrigue A,Dian C,Kapp U,Terradot L,Mandrand-Berthelot MA,Michaud-Soret I

更新日期：2006-11-01 00:00:00

abstract：:1) Isonitrile derivatives of synthetic polyamides, polyesters and polyalcohols, of polysaccharides such as cellulose, cross-linked dextran and agarose, linear dextran and of cross-linked and linear polyacrylamide were prepared. 2) Proteins and low-molecular-weight ligands were bound covalently to these materials by fo...

journal_title：Biochimie

authors： Goldstein L

更新日期：1980-01-01 00:00:00

abstract：:Total poly (A+) RNA has been extracted from rat pheochromocytoma and translated in vitro by means of a reticulocyte lysate system. We show that two antisera, prepared against pig kidney DOPA decarboxylase (DDC) or rat pheochromocytoma DDC, immunoprecipitate an in vitro synthetized 50 kDa polypeptide identified as DDC ...

journal_title：Biochimie

authors： Bruneau G,Krieger-Poullet M,Coge F,Borri-Voltattorni C,Gros F,Thibault J

更新日期：1990-01-01 00:00:00

abstract：:A 3-D model of the tyrosylable tRNA-like domain of the genomic brome mosaic virus RNAs was built by computer modelling based on solution probing of the molecule with different chemical and enzymatic reagents. This model encompasses four major structural domains, including two peculiar substructures oriented perpendicu...

journal_title：Biochimie

authors： Felden B,Florentz C,Westhof E,Giegé R

更新日期：1993-01-01 00:00:00

abstract：:Nucleic acid Aptamers are ligands that are selected by a process of molecular evolution to bind with high affinities and specificities to a specific target. Recently, an increasing number of aptamers have been selected against biomarkers expressed at the surface of human cells or infectious pathogens. This class of ta...

journal_title：Biochimie

authors： Cibiel A,Pestourie C,Ducongé F

更新日期：2012-07-01 00:00:00

abstract：:Here we report two novel 17-mer amidated linear peptides (TsAP-1 and TsAP-2) whose structures were deduced from cDNAs cloned from a venom-derived cDNA library of the Brazilian yellow scorpion, Tityus serrulatus. Both mature peptides were structurally-characterised following their location in chromatographic fractions ...

journal_title：Biochimie

authors： Guo X,Ma C,Du Q,Wei R,Wang L,Zhou M,Chen T,Shaw C

更新日期：2013-09-01 00:00:00

abstract：:The Escherichia coli protein synthesis initiation factor IF2 is a member of the large family of G-proteins. Along with translational elongation factors EF-Tu and EF-G and translational release factor RF-3, IF2 belongs to the subgroup of G-proteins that are part of the prokaryotic translational apparatus. The roles of ...

journal_title：Biochimie

authors： Larigauderie G,Laalami S,Nyengaard NR,Grunberg-Manago M,Cenatiempo Y,Mortensen KK,Sperling-Petersen HU

更新日期：2000-12-01 00:00:00

abstract：:Because of the ability of photosensitizers to induce specific photochemical reactions in vivo, leading to cell injury and death, many such molecules have been considered as therapeutic agents. Among them two classes of sensitizers, i.e. furocoumarins (psoralens) and porphyrins, are currently used for the photochemothe...

journal_title：Biochimie

authors： Moreno G

更新日期：1986-06-01 00:00:00

abstract：:The mechanism responsible for creating the division site in the right place at the right time in bacteria is unknown. It has been attributed to the formation of proteolipid domains in the cytoplasmic membrane surrounding the nucleoids. We interpret the growing evidence for this hypothesis by invoking hyperstructures, ...

journal_title：Biochimie

authors： Norris V,Fishov I

更新日期：2001-01-01 00:00:00

abstract：:Amyloid beta protein (beta/A4) is deposited in senile plaques of patients with Alzheimer's disease. This protein is derived from a larger membrane-associated protein, termed amyloid precursor protein (APP). The constitutive processing of APP occurs at the central portion of beta/A4, resulting in the release of large N...

journal_title：Biochimie

authors： Shimokawa M,Nakamura K,Maruyama K,Tagawa K,Miyatake T,Sugita H,Ishiura S,Suzuki K

更新日期：1993-01-01 00:00:00

abstract：:The oxidation of beta-D-glucose with glucose oxidase generally requires oxygen, which, under normal conditions is present at low concentrations in the reaction medium. Experiments show that glucose oxidase is no longer saturated by oxygen at enzyme concentrations greater than 0.4 mg.ml1. This is due to the decrease in...

journal_title：Biochimie

authors： Boudrant J,Cheftel C

更新日期：1975-01-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) and αvβ3 integrin are key molecules that actively participate in tumor angiogenesis and metastasis. Some integrin-blocking molecules are currently under clinical trials for cancer and metastasis treatment. However, the mechanism of action of such inhibitors is not completely u...

journal_title：Biochimie

authors： Montenegro CF,Salla-Pontes CL,Ribeiro JU,Machado AZ,Ramos RF,Figueiredo CC,Morandi V,Selistre-de-Araujo HS

更新日期：2012-08-01 00:00:00

abstract：BACKGROUND:Fatty acids (FAs), and especially monounsaturated FAs (MUFAs) stimulate GLP-1 release. However, lipotoxicity is indicated in GLP-1 secreting cells following long-term exposure to elevated levels of saturated FAs (SFAs) in vivo and in vitro, where in vitro studies indicate that cosupplementation with MUFAs co...

journal_title：Biochimie

authors： Ntika S,Thombare K,Aryapoor M,Kristinsson H,Bergsten P,Krizhanovskii C

更新日期：2019-04-01 00:00:00

abstract：:A depletion in long-chain polyunsaturated omega3 fatty acids may affect fuel homeostasis. In such a perspective, the present study deals mainly with the in vitro fate of D-[U-(14)C]glucose in hemidiaphragms, stretched soleus and plantaris muscle pieces obtained from normal and omega3-depleted rats (second generation) ...

journal_title：Biochimie

authors： Agascioglu E,Zhang Y,Sener A,Portois L,Chardigny JM,Malaisse WJ,Carpentier YA

更新日期：2007-03-01 00:00:00

abstract：:Two forms of baker's yease valyl-tRNA synthetase have been purified to apparent homogeneity by classical methods. It was demonstrated that one of the two forms of the enzyme originates from the other by proteolysis, the respective amounts of each form depending on the physiological state of the yeast. The species main...

journal_title：Biochimie

authors： Kern D,Giegé R,Robre-Saul S,Boulanger Y,Ebel JP

更新日期：1975-01-01 00:00:00

abstract：:Phosphatidic acid phosphohydrolase-1 (PAP-1) activity is reversibly inhibited by fatty acids and their acyl-CoA esters and it appears paradoxical that these effectors have been reported to increase the liver's esterification capacity by translocating the rate-limiting enzyme PAP-1 from cytosol to the endoplasmic retic...

journal_title：Biochimie

authors： Elabbadi N,Day CP,Gamouh A,Zyad A,Yeaman SJ

更新日期：2005-05-01 00:00:00

abstract：:In septic shock patients, alterations of plasma phospholipid fatty acid profile have never been described. The purpose of this monocentric, non-interventional, observational prospective study was to describe this fatty acid profile in the early phase of septic shock in intensive care unit. Thirty-seven adult patients ...

journal_title：Biochimie

authors： Rival T,Cinq-Frais C,Silva-Sifontes S,Garcia J,Riu B,Salvayre R,Genestal M,Caspar-Bauguil S

更新日期：2013-11-01 00:00:00

abstract：:The lysosomal aspartic protease cathepsin D (cath-D) is overexpressed and hyper-secreted by epithelial breast cancer cells. This protease is an independent marker of poor prognosis in breast cancer as it is correlated with the incidence of clinical metastasis. In normal cells, cath-D is localized in intracellular vesi...

journal_title：Biochimie

authors： Masson O,Bach AS,Derocq D,Prébois C,Laurent-Matha V,Pattingre S,Liaudet-Coopman E

更新日期：2010-11-01 00:00:00

abstract：:The Escherichia coli low molecular mass penicillin-binding proteins (PBP4, PBP5 and PBP6) are a group of penicillin-sensitive enzymes involved in the final stages of cell wall assembly. It has been suggested that these proteins may interact with the periplasmic face of the inner membrane via C-terminal amphiphilic alp...

journal_title：Biochimie

authors： Harris F,Phoenix DA

更新日期：1997-04-01 00:00:00