Abstract：
:Pleurocidin (GWGSFFKKAAHVGKHVGKAALTHYL-NH(2)), found in skin mucous secretions of the winter flounder Pleuronectes americanus, is known to possess a high potency and broad-spectrum antimicrobial peptide without cytotoxicity. In this study, to investigate the impact of pleurocidin on apoptotic progress, we observed morphological and physiological changes in Candida albicans. In cells exposed to pleurocidin, intracellular reactive oxygen species (ROS) which is a major cause of apoptosis were increased, and hydroxyl radicals were especially a large part of ROS. The increase of ROS induced oxidative stress and mitochondrial membrane depolarization which causes release of pro-apoptotic factors. Using FITC-VAD-FMK staining, we confirmed activation of yeast metacaspases which lead to apoptosis and phosphatidylserine externalization at early stage apoptosis was observed using annexin V FITC. In addition, pleurocidin induced-apoptotic cells underwent apoptotic morphological changes, showing the reduced cell size (low FSC) and enhanced intracellular density (high SSC) in flow cytometry dot plots. Under the influence of oxidative stress, DNA and nuclei were fragmented and condensed in cells, and they were visualized by 4',6-diamidino-2-phenylindole (DAPI) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. These apoptotic phenomena represent that oxidative stress by inducing pleurocidin must be an important factor of the apoptotic process in C. albicans.
journal_name
Biochimiejournal_title
Biochimieauthors
Cho J,Lee DGdoi
10.1016/j.biochi.2011.07.011subject
Has Abstractpub_date
2011-10-01 00:00:00pages
1873-9issue
10eissn
0300-9084issn
1638-6183pii
S0300-9084(11)00269-0journal_volume
93pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract：:Sialidase catalyzes the removal of sialic acids from glycoconjugates. Recently, medaka sialidase Neu1 has been cloned and its enzymatic properties were investigated. Although enzymatic properties of this sialidase, such as optimal pH and substrate specificity, exhibits high similarity with human NEU1, Neu1 physiologic...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2017.01.008
更新日期：2017-04-01 00:00:00
abstract：:Adipose tissue is an endocrine organ that plays an essential role in regulating several metabolic functions through the secretion of biological mediators called "adipokines". Dysregulation of adipokines plays a crucial role in obesity-related diseases. Adiponectin (APN) is the most abundant adipokine accounting for th...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2012.06.030
更新日期：2012-10-01 00:00:00
abstract：:Leishmania parasites cause two million new cases of leishmaniasis each year with several hundreds of millions of people at risk. Due to the paucity and shortcomings of available drugs, we have undertaken the crystal structure determination of a key enzyme from Leishmania major in hopes of creating a platform for the r...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2010.11.015
更新日期：2011-03-01 00:00:00
abstract：:mRNAs extracted from human pheochromocytoma were translated in vitro in a lysate of a rabbit reticulocytes. Two enzymes of the biosynthetic pathway of the catecholamines, tyrosine-hydroxylase (TH) and dopamine-beta-hydroxylase (DBH), were characterized as translation products after immunoprecipitation by specific anti...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(85)80198-x
更新日期：1985-06-01 00:00:00
abstract：:TcrX/Y is one of the twelve two component system (TCS) present in Mycobacterium tuberculosis. We have investigated the TcrX/Y interaction by in silico studies, pull down assay, radioactive phosphotransfer, surface plasmon resonance as well as crosstalk analysis of TcrY with TcrA - a non-cognate response regulator. Seq...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2009.11.009
更新日期：2010-03-01 00:00:00
abstract：:Plant sucrose transporters (SUTs) are functional as sucrose-proton-cotransporters with an optimal transport activity in the acidic pH range. Recently, the pH optimum of the Solanum tuberosum sucrose transporter StSUT1 was experimentally determined to range at an unexpectedly low pH of 3 or even below. Various research...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2013.07.028
更新日期：2013-11-01 00:00:00
abstract：:New strains of basidiomycetes producing extracellular laccases (Trametes ochracea 92-78, and Trametes hirsuta 56) have been found by screening of isolates of Trametes fungi. The laccases from T. hirsuta 56 and T. ochracea 92-78 as well as two laccases from previously found and described strains of basidiomycetes, name...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2004.08.005
更新日期：2004-09-01 00:00:00
abstract：:Isovaleryl-CoA dehydrogenase (IVD) catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA and the transfer of electrons to the electron transfer flavoprotein (ETF). Recombinant human IVD purifies with bound CoA-persulfide. A modified purification protocol was developed to isolate IVD without bound CoA-pers...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2014.11.007
更新日期：2015-01-01 00:00:00
abstract：:Kinetic studies indicate that glucose-6-phosphatase is a multifunctional enzyme. a) Phosphohydrolase activities. The mannose-6-phosphatase activity is low (Km = 8 mM, VM = 90 nmoles. min-1mg-1). The enzyme shows a strong affinity for glucose-6-phosphate (Km = 2.5 mM, VM = 220 nmoles.min-1mg-1). beta-glycerophosphate (...
journal_title：Biochimie
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:The secretome produced by cells within the bone marrow is significant to homeostasis. The bone marrow, a well-studied organ, has multiple niches with distinct roles for supporting stem cell functions. Thus, an understanding of mediators involved in the regulation of stem cells could serve as a model for clinical probl...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2018.05.018
更新日期：2018-12-01 00:00:00
abstract：:Explants from 7, 8, 9, 11, 13-day chick embryonic skin incorporating (3H) Uridine for different periods 1 hr, 3 or 4 hr and a chase with actinomycin) are studied with respect to free (F) or membrane bound (B) cytoplasmic polysomes and to RNA extracted from them. Polysome specific activity decreases at older stages but...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80226-x
更新日期：1976-01-01 00:00:00
abstract：:Several inositol isomers and in particular myo-inositol (MI) and D-chiro-inositol (DCI), were shown to possess insulin-mimetic properties and to be efficient in lowering post-prandial blood glucose. In addition, abnormalities in inositol metabolism are associated with insulin resistance and with long term microvascula...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2013.05.011
更新日期：2013-10-01 00:00:00
abstract：:Our previous researches showed that nitronyl nitroxyl derivatives, NNP and NNVP were good anti-oxidants and provided radioprotective effects in C6 cells. The objective of the present study is to investigate the possible antiviral effects and underlying pharmacological of the two nitronyl nitroxide radicals against CVB...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2012.05.015
更新日期：2012-09-01 00:00:00
abstract：:The rate of hydrolysis of long chain triglycerides by pure bovine pancreatic lipase has been determined in the presence of variable amounts of bile salts and colipase. Cofactor-free lipase is strongly inhibited by sodium taurodesoxycholate and by mixed bovine bile salts at concentrations higher than the critical micel...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(76)80572-x
更新日期：1975-01-01 00:00:00
abstract：:This paper provides an overview of the usefulness of the resonance Raman (RR) spectroscopy in the determination of the structural and electronic properties of heme(s) included in c-type cytochromes. It reviews the mode assignments presently available for heme c and includes recent RR data on the most important subclas...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/0300-9084(94)90145-7
更新日期：1994-01-01 00:00:00
abstract：:PA1b (Pea Albumin 1b) is a peptide toxin lethal for certain insects. This paper shows that the cultured insect cells Sf9 are sensitive to the toxin and display a high-affinity binding site for PA1b. Mammalian cells are not sensitive and no binding activity was detected. Signs of apoptosis of the Sf9 cells were observe...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2007.07.021
更新日期：2007-12-01 00:00:00
abstract：:The effects of DNA oligonucleotides complementary to potato spindle tuber viroid (PSTVd) on viroid infection were investigated. The oligonucleotides were used to form hybrids with PSTVd in the infection mixture. A 75% reduction of viroid infection was found when an oligonucleotide complementary to nucleotides 79-110 o...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(93)90026-o
更新日期：1993-01-01 00:00:00
abstract：:Insects have developed an efficient host defense against microorganisms, which involves humoral and cellular mechanisms. Numerous data highlight similarities between defense responses of insects and innate immunity of mammals. The fruit fly, Drosophila melanogaster, is a favorable model system for the analysis of the ...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2004.07.007
更新日期：2004-09-01 00:00:00
abstract：:Peroxisomal ABC transporters encoded by the ABCD genes are thought to participate in the import of specific fatty acids in the peroxisomal matrix. ABCD1 deficiency is associated with X-linked adrenoleukodystrophy (X-ALD), the most frequent peroxisomal disorder which is characterized by the accumulation of saturated ve...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2008.05.022
更新日期：2008-10-01 00:00:00
abstract：:Seryl-tRNA synthetases (SerRS) covalently attach serine to cognate tRNA(Ser). Atypical SerRSs, considerably different from canonical enzymes, have been found in methanogenic archaea. A crystal structure of methanogenic-type SerRS revealed a motif within the active site (serine ordering loop; SOL), which undergoes a no...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.06.013
更新日期：2011-10-01 00:00:00
abstract：:Homocysteine (HCY) is a degradation product of the methionine pathway. The B vitamins, in particular vitamin B12 and folate, are the primary nutritional determinant of HCY levels and therefore their deficiencies result in hyperhomocysteinaemia (HHCY). Prevalence of hyperhomocysteinemia (HHCY) and related dietary defic...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2016.04.009
更新日期：2016-07-01 00:00:00
abstract：:We have analyzed changes in the supercoiling of nucleoid DNA of murine fibroblasts cultured in the presence of hydralazine. The entire DNA attached to the nuclear matrix was extracted from the cells and sedimented in neutral sucrose density gradients containing ethidium bromide. Nucleoids from cells treated with hydra...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(98)80025-4
更新日期：1998-07-01 00:00:00
abstract：:Kinetic parameters characterizing the catalytic activities of enzymes are typically investigated in dilute solutions. However, in reality, these reactions occur in cells that, in addition to water and ions, are full of other macromolecules including proteins, nucleic acids, lipids, and metabolites. Such a crowded envi...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2020.07.009
更新日期：2020-09-01 00:00:00
abstract：:Cell cycle-related and expression elevated protein in tumor (CREPT) is emerging as a novel cancer-related gene that is dysregulated in many kinds of malignancies. However, the expression and biological role of CREPT in glioma remains unclear. In the present study, we aimed to explore the potential function and regulat...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2019.04.014
更新日期：2019-07-01 00:00:00
abstract：:The translation factor EF-P is required for efficient prokaryotic peptide bond synthesis on 70S ribosomes from fMet-tRNAfMet. This protein has been purified from Escherichia coli cells and the gene, efp, encoding it has been cloned and sequenced. We have isolated recombinant clones which overexpress a protein that co-...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(97)87619-5
更新日期：1997-01-01 00:00:00
abstract：:Interleukin-17 (IL-17) is produced exclusively by activated T cells and neutrophils, and stimulates osteoclastic bone resorption via osteoblasts by inducing the expression of "receptor activator of NF-kappaB (RANK) ligand" (RANKL). However, the direct effects of IL-17 on the differentiation of osteoclast precursors in...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2009.12.011
更新日期：2010-04-01 00:00:00
abstract：:Administration of beta-adrenergic agonists to domestic species can lead to skeletal muscle hypertrophy, probably by reducing the rate of myofibrillar protein breakdown. Myofibrillar breakdown is associated with the calcium-dependent proteinase system (calpains I,II and calpastatin) whose activity also changes during b...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/0300-9084(92)90125-x
更新日期：1992-03-01 00:00:00
abstract：:After exhaustive pronase digestion, purification by gel filtration and affinity chromatography on concanavalin A, three glycopeptide fractions were obtained from rat hemopexin. Two fractions (I and II) were concanavalin A non-reactive and one (III) was concanavalin A reactive. On the basis of carbohydrate composition,...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/s0300-9084(83)80083-2
更新日期：1983-03-01 00:00:00
abstract：:Conjugated linoleic acids (CLA) are positional and geometric dienoic isomers of linoleic acid. Dietary CLA supplementation leads to a drop in fat mass in various species, including in humans. The t10,c12-CLA isomer is responsible for this anti-obesity effect. The reduction of fat mass is especially dramatic in the mou...
journal_title：Biochimie
pub_type： 杂志文章,评审
doi：10.1016/j.biochi.2004.11.006
更新日期：2005-01-01 00:00:00
abstract：:In the intestine, NF-κB is the main transcription factor involved in the anti-inflammatory effect of glutamine and we previously demonstrated that glutamine via its conversion to glutamate diminished the p65 protein content in Caco-2/TC7 cell nuclei without affecting the stimulating effect of IL-1β on NF-κB [21]. Howe...
journal_title：Biochimie
pub_type： 杂志文章
doi：10.1016/j.biochi.2011.11.012
更新日期：2012-03-01 00:00:00
