Abstract:
:mRNAs extracted from human pheochromocytoma were translated in vitro in a lysate of a rabbit reticulocytes. Two enzymes of the biosynthetic pathway of the catecholamines, tyrosine-hydroxylase (TH) and dopamine-beta-hydroxylase (DBH), were characterized as translation products after immunoprecipitation by specific antisera and electrophoretic analysis. The precursor of TH is a polypeptide having a molecular mass of 62,000 identical to that found for the mature protein. The molecular mass of the precursor of DBH 73,000 while that of the mature form is 79,000. TH and DBH have been translated from mRNAs having sedimentation coefficients of 22S and 25S, respectively.
journal_name
Biochimiejournal_title
Biochimieauthors
Benlot C,Antreassian J,Henry JP,Legrand JC,Gros F,Thibault Jdoi
10.1016/s0300-9084(85)80198-xsubject
Has Abstractpub_date
1985-06-01 00:00:00pages
589-95issue
6eissn
0300-9084issn
1638-6183pii
S0300-9084(85)80198-Xjournal_volume
67pub_type
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