Effect of beta-agonists on expression of calpain and calpastatin activity in skeletal muscle.

abstract：:Glutathione, being a major intracellular redox regulator has been shown to be implicated in regulation of airway reactivity and inflammation. However, no study so far has investigated the effect of glutathione depletion/repletion during sensitization and challenge phases separately, which could provide an important in...

journal_title：Biochimie

authors： Nadeem A,Siddiqui N,Alharbi NO,Alharbi MM,Imam F,Sayed-Ahmed MM

更新日期：2014-08-01 00:00:00

abstract：:The molecular basis for the essential role of cholesterol in mammalian (and other cholesterol-requiring) cells has long been the object of intense interest. Cholesterol has been found to modulate the function of membrane proteins critical to cellular function. Current literature supports two mechanisms for this modula...

journal_title：Biochimie

authors： Yeagle PL

更新日期：1991-10-01 00:00:00

abstract：:Previous work from this laboratory has shown that T7 RNA polymerase outpaces ribosomes in vivo, generating naked mRNA stretches which may be nuclease-sensitive. In particular, lacZ transcripts synthesised this way are highly unstable and yield little beta-galactosidase. We have argued that most of these transcripts ar...

journal_title：Biochimie

authors： Lopez PJ,Dreyfus M

更新日期：1996-01-01 00:00:00

abstract：:The efficiency of oxidative phosphorylation was estimated in intact resting cells of Escherichia coli K 12, strain PA 601 (chl-s) and its chl-r mutants, all of them grown anaerobically in the presence of nitrate. The oxidation of endogenous NADH in intact chl-s cells was accompanied by the formation of ATP whatever th...

journal_title：Biochimie

authors： Giordano G,Rivière C,Azoulay E

更新日期：1977-01-01 00:00:00

abstract：:Kinetic studies indicate that glucose-6-phosphatase is a multifunctional enzyme. a) Phosphohydrolase activities. The mannose-6-phosphatase activity is low (Km = 8 mM, VM = 90 nmoles. min-1mg-1). The enzyme shows a strong affinity for glucose-6-phosphate (Km = 2.5 mM, VM = 220 nmoles.min-1mg-1). beta-glycerophosphate (...

journal_title：Biochimie

authors： Benedetto JP,Martel MB,Got R

更新日期：1979-01-01 00:00:00

abstract：:We report Raman spectra of various cholinesterases: lytic tetrameric forms (G4) obtained by tryptic digestion of asymmetric acetylcholinesterase (AChE) from Torpedo californica and Electrophorus electricus, a PI-PLC-treated dimeric form (G2) of AChE from T marmorata, and the soluble tetrameric form (G4) of butyrylchol...

journal_title：Biochimie

authors： Aslanian D,Grof P,Bon S,Masson P,Négrerie M,Chatel JM,Balkanski M,Taylor P,Massoulié J

更新日期：1991-11-01 00:00:00

abstract：:The solubilizing powers of taurocholate, taurochenodeoxycholate and tauroursodeoxycholate for monoolein and cholesterol, and the size of the bile salt-monoolein-cholesterol micelles have been determined. For the three bile salt species, the micellar size depends on the saturation with monoolein. As a result, for a giv...

journal_title：Biochimie

authors： Montet JC,Lindheimer M,Reynier MO,Crotte C,Bontemps R,Gerolami A

更新日期：1982-04-01 00:00:00

abstract：:Genome-editing technologies, in particular, CRISPR systems, are widely used for targeted regulation of gene expression and obtaining modified human and animal cell lines, plants, fungi, and animals with preassigned features. Despite being well described and easy to perform, the most common methods for construction and...

journal_title：Biochimie

authors： Filippova J,Matveeva A,Zhuravlev E,Stepanov G

更新日期：2019-12-01 00:00:00

abstract：:Murine L5178Y cell ribosomes were dissociated into subunits either with potassium chloride in the presence of puromycin or with the chelating agent EDTA. The proteins of ribosomal subunits obtained by these different methods were compared by means of bidimensional polyacrylamide gel electrophoresis. KCl-derived 60S an...

journal_title：Biochimie

authors： Longuet M,Auger-Buendia MA,Tavitian A

更新日期：1979-01-01 00:00:00

abstract：:The development of in vitro repair assays with human cell-free extracts led to new insights on the mechanism of excision of DNA damage which consists of incision/excision and repair synthesis/ligation. We have adapted the repair synthesis reaction with cells extracts incubated with damaged plasmid DNA performed in liq...

journal_title：Biochimie

authors： Salles B,Rodrigo G,Li RY,Calsou P

更新日期：1999-01-01 00:00:00

abstract：:We have previously shown that withdrawal of folic acid led to metabolic reprogramming and a less aggressive phenotype in a mouse cell model of triple-negative breast cancer (TNBC). Herein, we evaluate the effects of folic acid withdrawal on transcriptomic profiles in these cells. Murine cell lines were originally deri...

journal_title：Biochimie

authors： Kok DE,O'Flanagan CH,Coleman MF,Ashkavand Z,Hursting SD,Krupenko SA

更新日期：2020-06-01 00:00:00

abstract：:The effect of 7-chloro-4-nitrobenzofurazan on yeast 3-phosphoglycerate kinase causes a modification of one tyrosyl residue concomitantly with a total loss of activity of the enzyme. The modification is not accompanied by any significant conformational change. A total protection against inactivation is observed with th...

journal_title：Biochimie

authors： Roustan C,Fattoum A,Pradel LA

更新日期：1979-01-01 00:00:00

abstract：:In most cases, anti-protein aptamers are selected by systematic evolution of ligands by exponential enrichment (SELEX) using purified recombinant protein targets. Cell surface proteins, however, are not easy targets for SELEX due to the difficulties associated with their purification. Here, we developed a novel SELEX ...

journal_title：Biochimie

authors： Ohuchi SP,Ohtsu T,Nakamura Y

更新日期：2006-07-01 00:00:00

abstract：:Numerous studies have reported the beneficial effects of antioxidants in human diseases. Among their biological effects, a majority of antioxidants scavenge reactive radicals in the body, thereby reducing oxidative stress that is associated with the pathogenesis of many diseases. Antioxidant dendrimers are a new class...

journal_title：Biochimie

authors： Lee CY,Nanah CN,Held RA,Clark AR,Huynh UG,Maraskine MC,Uzarski RL,McCracken J,Sharma A

更新日期：2015-04-01 00:00:00

abstract：:Dyskeratosis congenita is an inherited syndrome characterised by mucocutaneous features, bone marrow failure, an increased risk of malignancy and other somatic abnormalities. There is a considerable range of clinical severity and in its occult form the disease may present as idiopathic aplastic anaemia. Genes responsi...

journal_title：Biochimie

authors： Vulliamy TJ,Dokal I

更新日期：2008-01-01 00:00:00

abstract：:Total poly (A+) RNA has been extracted from rat pheochromocytoma and translated in vitro by means of a reticulocyte lysate system. We show that two antisera, prepared against pig kidney DOPA decarboxylase (DDC) or rat pheochromocytoma DDC, immunoprecipitate an in vitro synthetized 50 kDa polypeptide identified as DDC ...

journal_title：Biochimie

authors： Bruneau G,Krieger-Poullet M,Coge F,Borri-Voltattorni C,Gros F,Thibault J

更新日期：1990-01-01 00:00:00

abstract：:We have previously identified a protein with Mr approximately 40,000 (p40) that binds with high specificity and affinity to the 5'-untranslated leaders of mitochondrial mRNAs in yeast. Here we show that this protein is abundant, comprising about 0.4% of total mitochondrial protein. p40 is present in a cytoplasmic (rho...

journal_title：Biochimie

authors： Dekker PJ,Papadopoulou B,Grivell LA

更新日期：1991-12-01 00:00:00

abstract：:RNA domain swapping typically demonstrates conservation of the native function of the domain in a non-native context. In contrast, we employed RNA engineering to demonstrate deviation of G-quadruplex (GQ) function that is contingent upon its context dependent location, which is opposite to their native functional role...

journal_title：Biochimie

authors： Bhattacharyya D,Morris MJ,Kharel P,Mirihana Arachchilage G,Fedeli KM,Basu S

更新日期：2017-06-01 00:00:00

abstract：:The structure difference between the free operator of the lac system d(GCTCACAAT).d(ATTGTGAGC) and the same operator complexed to the headpiece of the lac repressor has been investigated by 2-D-1H NMR spectroscopy in conjunction with molecular modelling in internal coordinates (JUMNA). The free and complexed operator ...

journal_title：Biochimie

authors： Gincel E,Lancelot G,Maurizot JC,Thuong NT,Vovelle F

更新日期：1994-01-01 00:00:00

abstract：:A discriminating system capable of recognizing the oxygenated sterols was investigated in human lymphocytes. After labelling entire cells with 25-hydroxy [3H] cholesterol (10 nM) the cytosol was ultracentrifuged on a linear sucrose density gradient. Bound 25-hydroxy [3H] cholesterol was located in a single peak with a...

journal_title：Biochimie

authors： Defay RE,Astruc ME,Roussillon S,Descomps B,Crastes De Paulet A

更新日期：1982-05-01 00:00:00

abstract：:Human telomeres are intrinsically dynamic structures, with multiple biological processes operating to generate substantial length heterogeneity. Processes that operate specifically at the terminus, that include the end-replication problem coupled with C-strand resection, result in gradual telomere erosion with ongoing...

journal_title：Biochimie

authors： Baird DM

更新日期：2008-01-01 00:00:00

abstract：:Nitrogen fixation in A. vinelandii and R. leguminosarum bacteroides shows identical characteristics with respect to the dependence on membrane energization, the sensitivity to uncouplers, the ATP/ADP-ratio, and the dependences on flavodoxinhydroquinone as electrondonor. Although we have been successful in preparing in...

journal_title：Biochimie

authors： Veeger C,Laane C,Scherings G,van Zeeland Wolbers L

更新日期：1978-01-01 00:00:00

abstract：:The oncogenic potential of the high-risk human papillomavirus (HPV) relies on the expression of genes specifying the E7 and E6 proteins. To investigate further the variation in oligomeric structure that has been reported for different E7 proteins, an HPV-18 E7 cloned from a Hispanic woman with cervical intraepithelial...

journal_title：Biochimie

authors： Liu S,Tian Y,Greenaway FT,Sun MZ

更新日期：2010-07-01 00:00:00

abstract：:A novel bgl1 gene, encoding GH3 family β-glucosidase from Penicillium verruculosum (PvBGL), was cloned and heterologously expressed in P. canescens RN3-11-7 (niaD-) strain under the control of the strong xylA gene promoter. The recombinant rPvBGL was purified and their properties were studied in comparison with those ...

journal_title：Biochimie

authors： Volkov PV,Rozhkova AM,Zorov IN,Sinitsyn AP

更新日期：2020-01-01 00:00:00

abstract：:In most cancer cells, the ATP necessary for survival and proliferation is derived from glycolysis rather than from oxidative phosphorylations (OXPHOS) even when oxygen supply would be adequate to sustain them. This phenomenon, named "aerobic glycolysis" by Warburg many years ago, can now be explained by a mechanism up...

journal_title：Biochimie

authors： Hervouet E,Simonnet H,Godinot C

更新日期：2007-09-01 00:00:00

abstract：:Phosphoenolpyruvate carboxykinase (PEPCK) is the key enzyme in glyceroneogenesis, an important metabolic pathway that functions to restrain the release of non-esterified fatty acids (NEFAs) from adipocytes. The antidiabetic drugs known as thiazolidinediones (TZDs) are thought to achieve some of their benefits by lower...

journal_title：Biochimie

authors： Glorian M,Duplus E,Beale EG,Scott DK,Granner DK,Forest C

更新日期：2001-10-01 00:00:00

abstract：:Phosphatidic acid phosphohydrolase-1 (PAP-1) activity is reversibly inhibited by fatty acids and their acyl-CoA esters and it appears paradoxical that these effectors have been reported to increase the liver's esterification capacity by translocating the rate-limiting enzyme PAP-1 from cytosol to the endoplasmic retic...

journal_title：Biochimie

authors： Elabbadi N,Day CP,Gamouh A,Zyad A,Yeaman SJ

更新日期：2005-05-01 00:00:00

abstract：:The formation of integral membrane voltage-gated ion channels by the initially soluble C-terminal channel polypeptide (CP) of the pore-forming colicins is a fruitful area for studies on membrane protein import. The dependence of CP import on specific membrane parameters can be better understood using liposomes and pla...

journal_title：Biochimie

authors： Zakharov SD,Cramer WA

更新日期：2002-05-01 00:00:00

abstract：:The bovine oxytocin precursor was expressed in Escherichia coli as a fusion protein by cloning the hormone encoding cDNA in frame behind the replicase gene of the RNA phage MS2. By step-wise extraction with different urea concentrations, the fusion protein was enriched in the 7 M urea fraction and further purified by ...

journal_title：Biochimie

authors： Nörenberg U,Brunath A,Richter D

更新日期：1988-01-01 00:00:00

abstract：:EPR spectroscopy in conjunction with oxidation-reduction potentiometry has been used to determine the half-reduction potentials of the four hemes of cytochrome c3. As predicted, the four hemes of cytochrome c3 have different mid-point potentials. The Em values are: Heme I,--284 mV; Heme II,--310 mV; Heme III,--324 mV ...

journal_title：Biochimie

authors： Dervartanian DV,Xavier AV,Gall JL

更新日期：1978-01-01 00:00:00