Studies on the effect of bile salt and colipase on enzymatic lipolysis. Improved method for the determination of pancreatic lipase and colipase.

abstract：:The published literature contains a number of examples of normally non-cytoplasmic proteins whose transport out of the cytoplasm is not completely abolished by drastic alterations to their routing signals (signal sequences, etc). Furthermore, there are numerous examples of cytoplasmic proteins that can be routed to an...

journal_title：Biochimie

authors： Pugsley AP

更新日期：1990-02-01 00:00:00

abstract：:The aim of the present work was to establish the thermodynamic and functional differences in the protein-protein interactions between the components of the P450-dependent mitochondrial (mit) and microsomal (mic) monooxygenase systems using 12 different isoforms of cytochromes P450 and two redox partners, NADPH-depende...

journal_title：Biochimie

authors： Yablokov EO,Sushko TA,Ershov PV,Florinskaya AV,Gnedenko OV,Shkel TV,Grabovec IP,Strushkevich NV,Kaluzhskiy LA,Usanov SA,Gilep AA,Ivanov AS

更新日期：2019-07-01 00:00:00

abstract：:Protein 3D structures support their biological functions. As the number of protein structures is negligible in regards to the number of available protein sequences, prediction methodologies relying only on protein sequences are essential tools. In this field, protein secondary structure prediction (PSSPs) is a mature ...

journal_title：Biochimie

authors： de Brevern AG

更新日期：2020-12-01 00:00:00

abstract：:Matrix metalloproteinase 9 (MMP-9) and interleukin-8 (IL-8) play major roles in tumor progression and invasion of breast cancer cells. The present study was undertaken to investigate the inhibitory mechanism of cell invasion by luteolin 8-C-β-fucopyranoside (named as LU8C-FP), a C-glycosylflavone, in human breast canc...

journal_title：Biochimie

authors： Park SH,Kim JH,Lee DH,Kang JW,Song HH,Oh SR,Yoon DY

更新日期：2013-11-01 00:00:00

abstract：:The lysosomal aspartic protease cathepsin D (cath-D) is overexpressed and hyper-secreted by epithelial breast cancer cells. This protease is an independent marker of poor prognosis in breast cancer as it is correlated with the incidence of clinical metastasis. In normal cells, cath-D is localized in intracellular vesi...

journal_title：Biochimie

authors： Masson O,Bach AS,Derocq D,Prébois C,Laurent-Matha V,Pattingre S,Liaudet-Coopman E

更新日期：2010-11-01 00:00:00

abstract：:Sialidase catalyzes the removal of sialic acids from glycoconjugates. Recently, medaka sialidase Neu1 has been cloned and its enzymatic properties were investigated. Although enzymatic properties of this sialidase, such as optimal pH and substrate specificity, exhibits high similarity with human NEU1, Neu1 physiologic...

journal_title：Biochimie

authors： Ryuzono S,Takase R,Kamada Y,Ikenaga T,Chigwechokha PK,Komatsu M,Shiozaki K

更新日期：2017-04-01 00:00:00

abstract：:Epithelial Ca(2+)-regulation, which governs cornified envelope formation in the skin epidermis and hair follicles, closely coincides with the expression of S100A3, filaggrin and trichohyalin, and the post-translational modification of these proteins by Ca(2+)-dependent peptidylarginine deiminases. This review summariz...

journal_title：Biochimie

authors： Kizawa K,Takahara H,Unno M,Heizmann CW

更新日期：2011-12-01 00:00:00

abstract：:The x-ray structure of the monotopic membrane protein (S)-mandelate dehydrogenase (MDH) from Pseudomonas putida reveals an inherent flexibility of its membrane binding segment that might be important for its biological activity. The surface of MDH exhibits a concentration of the positive charges on one side and the ne...

journal_title：Biochimie

authors： Sukumar N,Liu S,Li W,Mathews FS,Mitra B,Kandavelu P

更新日期：2018-11-01 00:00:00

abstract：:Labeling of 21-kDa material was observed when bovine brain soluble fraction was incubated with [adenylate-32P]NAD+ in the presence of GTP. The 21-kDa substrate, slightly smaller than C3 substrate in size, was labeled even without C3 exoenzyme. GTP could be replaced by nucleoside triphosphates other than ATP while ATP ...

journal_title：Biochimie

authors： Badruzzaman M,Miyaoka T,Terashima M,Yamada K,Ohno T,Yamamori C,Tsuchiya M,Shimoyama M

更新日期：1997-07-01 00:00:00

abstract：:Here we describe a convenient and robust ligase-independent method for construction of combinatorial and random mutant libraries. The homologous genes flanked by plasmid-derived DNA sequences are fragmented, and the random fragments are reassembled in a self-priming polymerase reaction to obtain chimeric genes. The pr...

journal_title：Biochimie

authors： An Y,Wu W,Lv A

更新日期：2010-08-01 00:00:00

abstract：:Bacteria of the Lactococcus (formerly lactic Streptococcus) genus are described to emphasize certain less well-known facts of importance in their successful use as dairy starters. Other streptococci (non-rod forms) are briefly characterized to emphasize their usefulness in industrial milk fermentations. ...

journal_title：Biochimie

authors： Sandine WE

更新日期：1988-04-01 00:00:00

abstract：:Sequence analysis of the Lutzomyia (Lu.) ayacuchensis salivary gland cDNA library identified a short peptide containing an RGD (Arg-Gly-Asp) sequence flanked by two cysteine residues in the C-terminal end as the most abundant transcript. In the present study, a recombinant protein of the RGD-containing peptide, design...

journal_title：Biochimie

authors： Kato H,Gomez EA,Fujita M,Ishimaru Y,Uezato H,Mimori T,Iwata H,Hashiguchi Y

更新日期：2015-05-01 00:00:00

abstract：:After treatment of L929 cells, a murine fibrosarcoma line, with tumor necrosis factor (TNF), a nuclear kappa B-like transcription factor is rapidly induced as identified by gel shift mobility assays using the kappa B-responsive sequence of the immunoglobulin or interleukin-6 (IL-6) genes as a DNA probe. When induction...

journal_title：Biochimie

authors： Patestos NP,Haegeman G,Vandevoorde V,Fiers W

更新日期：1993-01-01 00:00:00

abstract：:Beta-turmerin from turmeric (Curcuma longa) waste grits obtained after extraction of curcumin was purified by successive gel permeation chromatography. Homogeneity of beta-turmerin was confirmed by its movement as single band both in SDS-PAGE and as well as in native (basic) PAGE. The apparent molecular mass is approx...

journal_title：Biochimie

authors： Smitha S,Dhananjaya BL,Dinesha R,Srinivas L

更新日期：2009-09-01 00:00:00

abstract：:A methylene group of camphor is hydroxylated by a P450 monoxygenase system containing in addition to the cytochrome and flavoprotein dehydrogenase a two-iron, two-sulfur redox protein termed putidaredoxin. The regulatory control of electron transport equilibrium occurs via ligation of substrate and redoxin to the cyto...

journal_title：Biochimie

authors： Gunsalus IC,Sligar SG

更新日期：1976-01-01 00:00:00

abstract：:Stroke is a worldwide major cause of mortality and morbidity. Preclinical studies have identified over 1000 molecules with brain-protective properties. More than 200 clinical trials have evaluated neuroprotective candidates for ischemic stroke yet, to date almost all failed, leading to a re-analysis of treatment strat...

journal_title：Biochimie

authors： Blondeau N

更新日期：2016-01-01 00:00:00

abstract：:Hypertension is the leading cause of preventable mortality worldwide, contributing to over 9 million deaths per annum, predominantly owing to cardiovascular disease. The association of obesity, physical inactivity and alcohol with elevated blood pressure (BP) is firmly established. Weight loss or other dietary strateg...

journal_title：Biochimie

authors： Psara E,Pentieva K,Ward M,McNulty H

更新日期：2020-06-01 00:00:00

abstract：:Glycogen was described as a temporal storage molecule in rhodococci, interconnecting lipids and carbon availability. The Rhodococcus jostii ADP-glucose pyrophosphorylase (ADP-GlcPPase) kinetic and regulatory properties support this role. Curiously, the enzyme uses glucosamine-1P as alternative substrate. Herein, we re...

journal_title：Biochimie

authors： Cereijo AE,Alvarez HM,Iglesias AA,Asencion Diez MD

更新日期：2020-09-01 00:00:00

abstract：:Since 2012, the interest for TMEM165 increased due to its implication in a rare genetic human disease named TMEM165-CDG (Congenital Disorder(s) of Glycosylation). TMEM165 is a Golgi localized protein, highly conserved through evolution and belonging to the uncharacterized protein family 0016 (UPF0016). Although the pr...

journal_title：Biochimie

authors： Lebredonchel E,Houdou M,Potelle S,de Bettignies G,Schulz C,Krzewinski Recchi MA,Lupashin V,Legrand D,Klein A,Foulquier F

更新日期：2019-10-01 00:00:00

abstract：:Matrix metalloproteinases (MMPs) are a family of zinc endopeptidases that process growth factors, growth factor binding proteins, cell surface proteins, degrade extracellular matrix (ECM) components and thereby play a central role in tissue remodeling and tumor progression. Membrane-type matrix metalloproteinases (MT-...

journal_title：Biochimie

authors： Sounni NE,Noel A

更新日期：2005-03-01 00:00:00

abstract：:Structural studies on various electron transfer complexes involving the tetrahemic cytochrome c3 provided evidence that one of the hemes (heme 4) is the interacting site on the molecule. The reactivity of this particular heme is allocated to the positive charges found around the heme group which are strongly involved ...

journal_title：Biochimie

authors： Guerlesquin F,Dolla A,Bruschi M

更新日期：1994-01-01 00:00:00

abstract：:Organisms exposed to ionizing radiation (IR) undergo increases in the production of reactive oxygen species (ROS), which are determinant components in the induction of apoptosis. Sensitive to apoptosis gene (SAG) encodes a redox-inducible and apoptosis-protective antioxidant protein. This report demonstrates that the ...

journal_title：Biochimie

authors： Kim SY,Yang ES,Lee YS,Lee J,Park JW

更新日期：2011-02-01 00:00:00

abstract：:Analysis of the nucleotide sequence of 1,400 transfer RNAs has revealed the imprint of a prototypic genetic code in position 3-4-5 of the acceptor stem. It appears only in the transfer RNAs for the primordial amino acids ie those found by chemical condensation of a nitrogen-methane-water-ammonia mixture. The model for...

journal_title：Biochimie

authors： Möller W,Janssen GM

更新日期：1990-05-01 00:00:00

abstract：:Present knowledge of the different enzymatic steps of the denitrification chains in various bacteria, particularly Paracoccus denitrificans and Pseudomonas aeruginosa has been briefly reviewed. The question whether nitric oxide (NO), nitrous oxide (N2O) and other nitrogen derivatives are obligatory intermediates has b...

journal_title：Biochimie

authors： Henry Y,Bessières P

更新日期：1984-04-01 00:00:00

abstract：:Synthetic antiestrogens such as Tamoxifen (Nolvadex) are currently used to treat postmenopausal breast cancer. Their mechanism of action is reviewed at the cellular and molecular level, through data of the literature and the current view of the author. Synthetic antiestrogens are mostly acting directly on breast cance...

journal_title：Biochimie

authors： Rochefort H

更新日期：1982-02-01 00:00:00

abstract：:The vasoactive hormone bradykinin, its N-and C-terminal fragments and some structural analogues were studied by Circular Dichroism. Conformational features of the peptide can be detected by comparative analysis of the various CD spectra recorded as a function of aqueous pH, solvent and temperature. It is shown that th...

journal_title：Biochimie

authors： Lintner K,Fermandjian S,Regoli D

更新日期：1979-01-01 00:00:00

abstract：:Three different hydrogenases (isolated from Clostridium pasteurianum, Desulfovibrio desulfuricans strain Norway 4 and D. baculatus 9974) added to a suspension of TiO2 (anatase) powder are able to catalyze H2 evolution under band gap illumination of the semiconducting particles, and in the presence of EDTA or methanol ...

journal_title：Biochimie

authors： Cuendet P,Rao KK,Grätzel M,Hall DO

更新日期：1986-01-01 00:00:00

abstract：:Immobilization of enzymes by crystallization and subsequent cross-linking provide structures characterized by a regular three-dimensional molecular arrangement and a high packing density. Compared to randomly immobilized enzymes, such structures permit more detailed analysis of the variations in kinetic properties ari...

journal_title：Biochimie

authors： Tüchsen E,Hvidt A,Ottesen M

更新日期：1980-01-01 00:00:00

abstract：:An efficient phosphotriester methodology based on the use of condensing agents in the presence of several O-nucleophilic catalysts has been developed. ...

journal_title：Biochimie

authors： Efimov VA,Chakhmakhcheva OG

更新日期：1985-07-01 00:00:00

abstract：:Interleukin-17 (IL-17) is a pro-inflammatory cytokine produced primarily by a subset of CD4(+)T cells, called Th17 cells, that is involved in host defense, inflammation and autoimmune disorders. The two most structurally related IL-17 family members, IL-17A and IL-17F, form homodimeric (IL-17A/A, IL-17F/F) and heterod...

journal_title：Biochimie

authors： Adachi H,Ishiguro A,Hamada M,Sakota E,Asai K,Nakamura Y

更新日期：2011-07-01 00:00:00