Resonance Raman spectroscopy of c-type cytochromes.

abstract：:In the intestine, NF-κB is the main transcription factor involved in the anti-inflammatory effect of glutamine and we previously demonstrated that glutamine via its conversion to glutamate diminished the p65 protein content in Caco-2/TC7 cell nuclei without affecting the stimulating effect of IL-1β on NF-κB [21]. Howe...

journal_title：Biochimie

authors： Lesueur C,Bôle-Feysot C,Bekri S,Husson A,Lavoinne A,Brasse-Lagnel C

更新日期：2012-03-01 00:00:00

abstract：:The inhibition rates and spectral characteristics of 2 probes specific for the active-site serine residue of proteases were examined for evidence of conformational change of the proteases upon their binding to alpha 2-macroglobulin (alpha 2M). Elastase, chymotrypsin, trypsin, and plasmin were reacted with (7-nitrobenz...

journal_title：Biochimie

authors： Martini JL,Pochon F

更新日期：1989-03-01 00:00:00

abstract：:The effect of adenosine was tested on the energetic metabolism of fed rat liver cells after isolation. The cells were incubated in a buffered saline medium with glucose (5 mM) and adenosine (1 mM) for 30 minutes at 37 degrees C. This increased the concentration of the adenylic nucleotides ATP (+57 per cent, ADP (+39 p...

journal_title：Biochimie

authors： Marchand JC,Lavoinne A,Giroz M,Matray F

更新日期：1979-01-01 00:00:00

abstract：:The ascomycete Cladosporium herbarum is a prominent fungal inducer of Type I allergy. The only major allergen identified so far is Cla h 8, a NADP-dependent mannitol dehydrogenase (MtDH). MtDH, a cytoplasmic protein of 28.5kDa, belongs to the Short chain Dehydrogenases/Reductases (SDR), acting as a NADP-dependent oxid...

journal_title：Biochimie

authors： Nüss D,Goettig P,Magler I,Denk U,Breitenbach M,Schneider PB,Brandstetter H,Simon-Nobbe B

更新日期：2010-08-01 00:00:00

abstract：:Analysis of the nucleotide sequence of 1,400 transfer RNAs has revealed the imprint of a prototypic genetic code in position 3-4-5 of the acceptor stem. It appears only in the transfer RNAs for the primordial amino acids ie those found by chemical condensation of a nitrogen-methane-water-ammonia mixture. The model for...

journal_title：Biochimie

authors： Möller W,Janssen GM

更新日期：1990-05-01 00:00:00

abstract：:We present an approach for designing a knowledge-based system, called Sequence Acquisition In Context (SAIC), that will be able to cooperate with a biologist in the analysis of DNA sequences. The main task of the system is the acquisition of the expert knowledge that the biologist uses for solving ambiguities from gel...

journal_title：Biochimie

authors： Brézillon PJ,Zaraté P,Saci F

更新日期：1993-01-01 00:00:00

abstract：:Reuber H 35 hepatoma cells were synchronized by transfer in a serum free medium. Growth was re-initiated by addition of serum. Under these conditions DNA synthesis exhibited a maximum after 24 hours. Chromatin non-histone proteins prepared from cells at various phases of the cell cycle were incubated with [gamma-32P] ...

journal_title：Biochimie

authors： Defer N,Tichonicky L,Kruh J,Zoutewelle G,Van Wijk R

更新日期：1979-01-01 00:00:00

abstract：:We identified a T-to-C mutation 2 nucleotides (nt) upstream from the AG in a GT-AG intron between exons 2 and 3 in the human T-cell leukemia virus type I (HTLV-I) tax mRNA. This mutation resulted in the preferential usage of an alternative splice site, causing a 75-nt elongation of tax mRNA and reduced production of v...

journal_title：Biochimie

authors： Ohsugi T

更新日期：2006-02-01 00:00:00

abstract：:Four crystal structures of transfer RNA molecules were refined at 3 A resolution with the inclusion of the solvent molecules found in the difference maps: yeast tRNA-phe in the orthorhombic form, yeast tRNA-phe in the monoclinic form and yeast tRNA-asp in the A and B forms. Over 100 solvent molecules were located in e...

journal_title：Biochimie

authors： Westhof E,Dumas P,Moras D

更新日期：1988-02-01 00:00:00

abstract：:Human Rad51 (HsRad51), a key element of the homologous recombination repair pathway, is related to the resistance of cancer cells to chemo- and radio-therapies. This protein is thus a good target for the development of anti-cancer treatments. We have searched for new inhibitors directed against HsRad51 using the Syste...

journal_title：Biochimie

authors： Martinez SF,Renodon-Cornière A,Nomme J,Eveillard D,Fleury F,Takahashi M,Weigel P

更新日期：2010-12-01 00:00:00

abstract：:We have analyzed changes in the supercoiling of nucleoid DNA of murine fibroblasts cultured in the presence of hydralazine. The entire DNA attached to the nuclear matrix was extracted from the cells and sedimented in neutral sucrose density gradients containing ethidium bromide. Nucleoids from cells treated with hydra...

journal_title：Biochimie

authors： Weglarz L,Koceva-Chyła A

更新日期：1998-07-01 00:00:00

abstract：:Dysfunction in lipid metabolism may result in a decrease in hepatic autophagy, which contributes to the pathogenesis of non-alcoholic steatohepatitis. ATP-binding cassette transporter A1 transports free cholesterol and phospholipids to apolipoprotein A-I (apoA-I) to form nascent high-density lipoprotein particles. Res...

journal_title：Biochimie

authors： Rao X,Wang Y

更新日期：2019-10-01 00:00:00

abstract：:DNA replication in Escherichia coli is controlled at the initiation stage, possibly by regulation of the essential activity of DnaA protein. The cellular membrane has long been hypothesized to be involved in chromosomal replication. Accumulating evidence, both in vitro and in vivo, that supports the importance of memb...

journal_title：Biochimie

authors： Crooke E

更新日期：2001-01-01 00:00:00

abstract：:Dyskeratosis congenita is an inherited syndrome characterised by mucocutaneous features, bone marrow failure, an increased risk of malignancy and other somatic abnormalities. There is a considerable range of clinical severity and in its occult form the disease may present as idiopathic aplastic anaemia. Genes responsi...

journal_title：Biochimie

authors： Vulliamy TJ,Dokal I

更新日期：2008-01-01 00:00:00

abstract：:Thraustochytrium is a marine protist producing a specific profile of nutritionally important fatty acids, including very long chain polyunsaturated fatty acids (VLCPUFAs) docosahexaenoic acid (DHA, 22:6n-3), even chain saturated fatty acids (SFAs) palmitic acid (16:0), and odd chain SFAs pentadecanoic acid (15:0). To ...

journal_title：Biochimie

authors： Zhao X,Qiu X

更新日期：2018-01-01 00:00:00

abstract：:In vertebrates, mannose 6-phosphate receptors [MPR300 (Mr 300 kDa) and MPR46 (Mr 46 kDa)] are highly conserved transmembrane glycoproteins that mediate transport of lysosomal enzymes to lysosomes. Our studies have revealed the appearance of these putative receptors in invertebrates such as the molluscs and deuterostom...

journal_title：Biochimie

authors： Visa M,Hammer E,Völker U,Koliwer-Brandl H,Kelm S,Nadimpalli SK

更新日期：2012-05-01 00:00:00

abstract：:Two beta-D-glycosidases have been purified from the digestive juice of Achatina balteata by acetone fractionation, ion exchange chromatography through DEAE-Sephadex A-50, ammonium sulphate fractionation and gel chromatography through Sephadex G-200. The preparations are homogeneous by p/lyacrylamide gel electrophoresi...

journal_title：Biochimie

authors： Colas B,Attias J

更新日期：1977-01-01 00:00:00

abstract：:Alteration of the epigenome is associated with a wide range of human diseases. Therefore, deciphering the pathways that regulate the epigenetic modulation of gene expression is a major milestone for the understanding of diverse biological mechanisms and subsequently human pathologies. Although often evoked, little is ...

journal_title：Biochimie

authors： Ottaviani A,Gilson E,Magdinier F

更新日期：2008-01-01 00:00:00

abstract：:The action of indomethacin on the lactogenic activity of prolactin has been evaluated usind the technique of rabbit mammary gland organ culture. Indomethacin is totally unable to inhibit prolactin action as estimated by lactose synthetase activity and casein synthesis. These data suggest, as opposed to previous works...

journal_title：Biochimie

authors： Houdebine LM,Lacroix MC

更新日期：1980-01-01 00:00:00

abstract：:Using a new extended set of 13 amber suppressors in E coli, systematic amino-acid replacements were performed at positions 104(E) and 238(G) of TEM-1 beta-lactamase from PUC19. The enzyme is tolerant to most substitutions tested at position 104. Missense revertants E104K, E104S or E104Y exhibited only minor changes in...

journal_title：Biochimie

authors： Lenfant F,Labia R,Masson JM

更新日期：1990-06-01 00:00:00

abstract：:Botulinum neurotoxins (BoNT, serotypes A-G) and tetanus neurotoxin (TeNT) are bacterial proteins that comprise a light chain (M(r) approximately 50) disulfide linked to a heavy chain (M(r) approximately 100). By inhibiting neurotransmitter release at distinct synapses, these toxins cause two severe neuroparalytic dise...

journal_title：Biochimie

authors： Humeau Y,Doussau F,Grant NJ,Poulain B

更新日期：2000-05-01 00:00:00

abstract：:Acetylcholinesterase (AChE) has been identified and purified from the venom of desert cobra (W aegyptia) to apparent homogeneity using a TSK G 3000 SW gel filtration column and a Mono Q anion-exchange column. AChE was purified to homogeneity as established by sodium dodecylsulfate/polyacrylamide gel electrophoresis. T...

journal_title：Biochimie

authors： Duhaiman AS,Alhomida AS,Rabbani N,Kamal MA,al-Jafari AA

更新日期：1996-01-01 00:00:00

abstract：:In recent years the chaperone HSC-70 has become a target for drug design with a strong focus in anticancer therapies. In our study of possible inhibitors of HSC-70 enzymatic activity we screened compounds by NMR as well as X-ray crystallography. As part of our screening efforts we crystallized the human HSC-70 ATP bin...

journal_title：Biochimie

authors： Zhang Z,Cellitti J,Teriete P,Pellecchia M,Stec B

更新日期：2015-01-01 00:00:00

abstract：:Epigenetic alterations are involved in every step of carcinogenesis. The development of chromatin-modifying agents (CMAs) has provided the ability to fight cancer by reversing these alterations. Currently, four CMAs have been approved for cancer treatment; two DNA demethylating agents and two deacetylase inhibitors. A...

journal_title：Biochimie

authors： Seidel C,Florean C,Schnekenburger M,Dicato M,Diederich M

更新日期：2012-11-01 00:00:00

abstract：:Nucleolin (NCL) is one of the most abundant non ribosomal protein of the nucleolus where it plays a central role in polymerase I transcription. NCL is also found outside of the nucleolus, in the nucleoplasm, cytoplasm as well as on the cell membrane. It acts in all cell compartments to control cellular homeostasis and...

journal_title：Biochimie

authors： Berger CM,Gaume X,Bouvet P

更新日期：2015-06-01 00:00:00

abstract：:Our study highlights the tight relationship between protein binding to monolayers and the phase-state of the phospholipids. Interaction of mitochondrial creatine kinase with phospholipidic membranes was analysed using a two-phase monolayer system containing anionic phospholipids under chain mismatch conditions. Monola...

journal_title：Biochimie

authors： Vernoux N,Maniti O,Marcillat O,Vial C,Granjon T

更新日期：2009-06-01 00:00:00

abstract：:The antiviral activity of L-nucleoside analogs depends in part on the enantioselectivity of nucleoside kinases which catalyse their monophosphorylation. The substrate properties of human recombinant deoxycytidine kinase (dCK) and human recombinant deoxyguanosine kinase (dGK) with respect to L-adenosine and L-guanosine...

journal_title：Biochimie

authors： Gaubert G,Gosselin G,Boudou V,Imbach JL,Eriksson S,Maury G

更新日期：1999-11-01 00:00:00

abstract：:Cotranslational translocation of exportable proteins across the RER membrane prior to their release into the extracellular space has been essentially described by use of canine pancreatic microsomal membranes. Intracisternal segregation of nascent secretory proteins was observed to be irreversible and proteolytic remo...

journal_title：Biochimie

authors： Scheele GA

更新日期：1988-09-01 00:00:00

abstract：:The adaptive response appears in E. coli after exposure to low levels of alkylating agents. This system is under the positive control of the ada gene. At least two enzymes are induced during the response: 3-methyladenine DNA glycosylase II and O6-methylguanine DNA methyltransferase. The latter is also the product of t...

journal_title：Biochimie

authors： Defais M

更新日期：1985-03-01 00:00:00

abstract：:The ability of asialo-thyroglobulin to bind the thyroid RHL-1 subunit of the asialoglycoprotein receptor has been investigated. Ligand blot assays show that the recombinant carbohydrate recognition domain of the thyroid RHL-1 subunit specifically interacts with rat desialated thyroglobulin. Moreover, RT-PCR and Wester...

journal_title：Biochimie

authors： Pacifico F,Liguoro D,Acquaviva R,Formisano S,Consiglio E

更新日期：1999-05-01 00:00:00