Mitochondrial creatine kinase interaction with heterogeneous monolayers: Effect on lipid lateral organization.

abstract：:The study of modified nucleoside contributions to RNA chemistry, structure and function has been thwarted by the lack of a site-selected method of incorporation which is both versatile and adaptable to present synthetic technologies. A reproducible and versatile site-selected incorporation of nine differently modified...

journal_title：Biochimie

authors： Agris PF,Malkiewicz A,Kraszewski A,Everett K,Nawrot B,Sochacka E,Jankowska J,Guenther R

更新日期：1995-01-01 00:00:00

abstract：:The human ribosomal protein SA, known also as a precursor of the cell-surface laminin receptor, LAMR, is a protein of the 40S ribosomal subunit. It is homologous to eubacterial ribosomal protein S2p, but has a eukaryote-specific C-terminal domain (CTD) that is responsible in LAMR for the binding of laminin as well as ...

journal_title：Biochimie

authors： Malygin AA,Babaylova ES,Loktev VB,Karpova GG

更新日期：2011-03-01 00:00:00

abstract：:The effects of thermal injury (72 h post-injury), 72 h-partial (20% less food) or full starvation on the regulation of phosphofructokinase in the mucosa of rat small intestine were studied. Thermal injury and 72 h-partial or full starvation decreased the activity ratio v0.5/V, but the ratios obtained for thermally inj...

journal_title：Biochimie

authors： Khoja SM,Ardawi SM

更新日期：1987-01-01 00:00:00

abstract：:Phosphorus metabolites and intracellular pH have been examined in the slime mold Dictyostelium discoideum by non-destructive 31P-NMR measurements. In a spectrum from a suspension of aerobic amoebae, the major peaks are inorganic phosphate, nucleotide di- and triphosphates. In the corresponding perchloric acid extract,...

journal_title：Biochimie

authors： Satre M,Klein G,Martin JB

更新日期：1986-12-01 00:00:00

abstract：:An apparatus for chemi- and bioluminescence measurements is described. The main features are in high sensitivity: for example, 5 10(-12) M adenosine triphosphate, 5 10(-7) M glucose, 2 10(-9) M perhydrol and 4 10(-13) M peroxidase are easily detected; its fast mixing time and the possibility of injecting a reactant, a...

journal_title：Biochimie

authors： Arrio B,Lecuyer B,Dupaix A,Volfin P,Jousset M,Carrette A

更新日期：1980-01-01 00:00:00

abstract：:In the bacterium Escherichia coli, H-NS-(H1, H1a) is a heat-stable protein with a molecular mass of 15.5 kDa involved in nucleoid organisation and gene regulation linked to certain signal transduction pathways. We have shown that, following addition of preparations of everted inner membrane vesicles, heat-stable cleav...

journal_title：Biochimie

authors： Goldberg MD,Canvin JR,Freestone P,Andersen C,Laoudj D,Williams PH,Holland IB,Norris V

更新日期：1997-06-01 00:00:00

abstract：:A neutral protease, produced by Staphylococcus aureus, strain V8, was purified by precipitation with ammonium sulfate and acetone, and by chromatography on D.E.A.E.-cellulose. The purified protein is homogeneous by acrylamide gel electrophoresis. The molecular weight of the enzyme is estimated to be 28.000 daltons by ...

journal_title：Biochimie

authors： Saheb SA

更新日期：1976-01-01 00:00:00

abstract：:rBmTI-A is a recombinant serine protease inhibitor that belongs to the Kunitz-BPTI family and that was cloned from Rhipicephalus microplus tick. rBmTI-A has inhibitory activities on bovine trypsin, human plasma kallikrein, human neutrophil elastase and plasmin with dissociation constants in nM range. It is characteriz...

journal_title：Biochimie

authors： Bomediano Camillo LM,Ferreira GC,Duran AFA,da Silva FRS,Garcia W,Scott AL,Sasaki SD

更新日期：2020-12-24 00:00:00

abstract：:Interferons (IFNs) were considered for the treatment of patients with multiple sclerosis (MS) after the demonstration, based on small studies, of the efficacy of type IFN beta in decreasing the frequency of exacerbations in relapsing-remitting multiple sclerosis when administered intrathecally, subcutaneously, or intr...

journal_title：Biochimie

authors： Tourbah A,Lyon-Caen O

更新日期：2007-06-01 00:00:00

abstract：:The x-ray structure of the monotopic membrane protein (S)-mandelate dehydrogenase (MDH) from Pseudomonas putida reveals an inherent flexibility of its membrane binding segment that might be important for its biological activity. The surface of MDH exhibits a concentration of the positive charges on one side and the ne...

journal_title：Biochimie

authors： Sukumar N,Liu S,Li W,Mathews FS,Mitra B,Kandavelu P

更新日期：2018-11-01 00:00:00

abstract：:A variety of species of GPDH undergo acylation at two of the four active cystein sites per mole of tetrameric enzyme. This reaction requires tightly bound NAD+, a situation restricted to two of the four NAD sites per tetramer. S leads to N acyl transfer from cysteins to lysine in the diacyl enzyme yields an inactive e...

journal_title：Biochimie

authors： Malhotra OP,Bernhard SA,Seydoux F

更新日期：1981-02-01 00:00:00

abstract：:Although a 30 kDa polypeptide frequently is seen by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of post mortem (pm) skeletal muscle and in turn is used as an indicator of proteolysis, its origin has not been conclusively identified. We used antibodies to selected myofibrillar proteins...

journal_title：Biochimie

authors： Ho CY,Stromer MH,Robson RM

更新日期：1994-01-01 00:00:00

abstract：:The variable manifestation of phenotypes that occur in patients with neurofibromatosis type 1 (NF1) includes benign and malignant neurocutaneous tumors for which no adequate treatment exists. Cell-based screening of known bioactive compounds library identified the protein phosphatase 2A (PP2A) inhibitor Cantharidin an...

journal_title：Biochimie

authors： Semenova G,Stepanova DS,Deyev SM,Chernoff J

更新日期：2017-04-01 00:00:00

abstract：:Human mitochondrial aminoacyl-tRNA synthetases (mt-aaRSs), the enzymes which esterify tRNAs with the cognate specific amino acid, form mainly a different set of proteins than those involved in the cytosolic translation machinery. Many of the mt-aaRSs are of bacterial-type in regard of sequence and modular structural o...

journal_title：Biochimie

authors： Messmer M,Blais SP,Balg C,Chênevert R,Grenier L,Lagüe P,Sauter C,Sissler M,Giegé R,Lapointe J,Florentz C

更新日期：2009-05-01 00:00:00

abstract：:A novel approach to genetic sequence analysis is presented. This approach, based on compression of algorithms, has been launched simultaneously by Grumbach and Tahi, Milosavljevic and Rivals. To reduce the description of an object, a compression algorithm replaces some regularities in the description by special codes....

journal_title：Biochimie

authors： Rivals E,Dauchet M,Delahaye JP,Delgrange O

更新日期：1996-01-01 00:00:00

abstract：:About 150 participants attended the symposium organised at the Palais de la Bourse in Bordeaux, France on September 22-23, 2017. Thirty speakers from all over the world delivered lectures covering selection processes, aptamer chemistry and innovative applications of these powerful tools that display major advantages o...

journal_title：Biochimie

authors： Toulmé JJ,Azéma L,Darfeuille F,Dausse E,Durand G,Paurelle O

更新日期：2018-02-01 00:00:00

abstract：:Due to their function as adapters in translation, tRNA molecules share a common structural organization in all kingdoms and organelles with ribosomal protein biosynthesis. A typical tRNA has a cloverleaf-like secondary structure, consisting of acceptor stem, D-arm, anticodon arm, a variable region, and T-arm, with an ...

journal_title：Biochimie

authors： Wende S,Platzer EG,Jühling F,Pütz J,Florentz C,Stadler PF,Mörl M

更新日期：2014-05-01 00:00:00

abstract：:When colonizing the digestive tract of mono-associated rats, Ruminococcus gnavus E1 - a bacterium isolated from human faeces - produced a trypsin-dependent anti-Clostridium perfringens substance collectively named Ruminococcin C (RumC). RumC was isolated from the caecal contents of E1-monocontaminated rats and found t...

journal_title：Biochimie

authors： Crost EH,Ajandouz EH,Villard C,Geraert PA,Puigserver A,Fons M

更新日期：2011-09-01 00:00:00

abstract：:alpha-Latrotoxin, a protein toxin present in the venom of black widow spider, interacts with membrane receptors of neurons and other secretory cells to stimulate exocytosis. Two types of receptors have been identified and cloned. Our attention has been focused on the calcium independent receptor, a G-protein coupled r...

journal_title：Biochimie

authors： Pescatori M,Grasso A

更新日期：2000-09-01 00:00:00

abstract：:We have analysed the complete sequence of the Escherichia coli K12 isolate MG1655 genome for chromatin-associated protein binding sites, and compared the predicted location of predicted sites with experimental expression data from 'DNA chip' experiments. Of the dozen proteins associated with chromatin in E. coli, only...

journal_title：Biochimie

authors： Ussery D,Larsen TS,Wilkes KT,Friis C,Worning P,Krogh A,Brunak S

更新日期：2001-02-01 00:00:00

abstract：:Insect prophenoloxidase (PPO) is an essential innate immunity protein to induce pathogen into melanization. In Bombyx mori, pro-phenoloxidase-activating enzyme (PPAE) can directly cleave and activate PPO. However, PPO in Manduca sexta cannot be cleaved into active phenoloxidase (PO) by serine proteases unless cofactor...

journal_title：Biochimie

authors： Lu A,Li X,Hillyer JF,Beerntsen BT,Söderhäll K,Ling E

更新日期：2014-07-01 00:00:00

abstract：:The wheat germ translation initiation factor 2 (WGeIF-2) was isolated in a homogeneous state by an efficient procedure and characterized. Its molecular mass, as determined by a gel-filtration method is approximately 150,000 Da. According to SDS-PAGE WGeIF-2 consists of four subunits with M(r) 37,000 (alpha), 40,000 (b...

journal_title：Biochimie

authors： Shaikhin SM,Smailov SK,Lee AV,Kozhanov EV,Iskakov BK

更新日期：1992-05-01 00:00:00

abstract：:The 11 kb las locus, present on the 50 kb plasmid pCIM1, specifies the production of the lantibiotic lactocin S in Lactobacillus sakei L45. The gene cluster is organized into two oppositely orientated operons, lasAMNTUVPJW (lasA-W) and lasXY, the former of which contains the biosynthetic, immunity and transport genes....

journal_title：Biochimie

authors： Rawlinson EL,Nes IF,Skaugen M

更新日期：2002-05-01 00:00:00

abstract：:Two forms of baker's yease valyl-tRNA synthetase have been purified to apparent homogeneity by classical methods. It was demonstrated that one of the two forms of the enzyme originates from the other by proteolysis, the respective amounts of each form depending on the physiological state of the yeast. The species main...

journal_title：Biochimie

authors： Kern D,Giegé R,Robre-Saul S,Boulanger Y,Ebel JP

更新日期：1975-01-01 00:00:00

abstract：:The use of electrospun scaffolds for neural tissue engineering applications allows a closer mimicry of the native tissue extracellular matrix (ECM), important for the transplantation of cells in vivo. Moreover, the role of the electrospun fiber mat topography on neural stem cell (NSC) differentiation remains to be com...

journal_title：Biochimie

authors： Garrudo FFF,Mikael PE,Xia K,Silva JC,Ouyang Y,Chapman CA,Hoffman PR,Yu Y,Han X,Rodrigues CAV,Cabral JMS,Morgado J,Ferreira FC,Linhardt RJ

更新日期：2021-01-07 00:00:00

abstract：:Apoptosis is the physiological process by which unwanted cells in an organism are killed. Bcl-2, a membrane-bound cytoplasmic protein, and its close relative Bcl-xL, are both effective inhibitors of apoptosis induced by a wide variety of stimuli in many different cell types. In a previous study, we reported that suppr...

journal_title：Biochimie

authors： Cherbonnel-Lasserre C,Dosanjh MK

更新日期：1997-10-01 00:00:00

abstract：:Oxidative stress is the main mechanism behind efficient disinfectants, causing damage in bacterial macromolecules. Importantly, bacteria activate resistance mechanisms in response to damage generated by oxidative stress. Strategies allowing pathogens to survive oxidative stress are highly conserved among microorganism...

journal_title：Biochimie

authors： Cabezas CE,Laulié AM,Briones AC,Pardo-Esté C,Lorca DE,Cofré AA,Morales EH,Mora AY,Krüger GI,Bueno SM,Hidalgo AA,Saavedra CP

更新日期：2021-01-01 00:00:00

abstract：:The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central ner...

journal_title：Biochimie

authors： Sarbu M,Raab S,Henderson L,Fabris D,Vukelić Ž,Clemmer DE,Zamfir AD

更新日期：2020-03-01 00:00:00

abstract：:A discriminating system capable of recognizing the oxygenated sterols was investigated in human lymphocytes. After labelling entire cells with 25-hydroxy [3H] cholesterol (10 nM) the cytosol was ultracentrifuged on a linear sucrose density gradient. Bound 25-hydroxy [3H] cholesterol was located in a single peak with a...

journal_title：Biochimie

authors： Defay RE,Astruc ME,Roussillon S,Descomps B,Crastes De Paulet A

更新日期：1982-05-01 00:00:00

abstract：:Four full-length cDNAs encoding the precursors of four K(+)-toxin-like peptides (named BmKK(1), BmKK(2), BmKK(3) and BmmKK(4), respectively) were first isolated from a venom gland cDNA library of the Chinese scorpion Buthus martensii Karsch. The deduced precursors of BmKK(1), BmKK(2) and BmKK(3) are all made of 54 ami...

journal_title：Biochimie

authors： Zeng XC,Peng F,Luo F,Zhu SY,Liu H,Li WX

更新日期：2001-09-01 00:00:00