Abstract:
:Four full-length cDNAs encoding the precursors of four K(+)-toxin-like peptides (named BmKK(1), BmKK(2), BmKK(3) and BmmKK(4), respectively) were first isolated from a venom gland cDNA library of the Chinese scorpion Buthus martensii Karsch. The deduced precursors of BmKK(1), BmKK(2) and BmKK(3) are all made of 54 amino acid residues including a signal peptide of 23 residues, and a mature toxin of 31 residues with three disulfide bridges. The precursor of BmKK(4) is composed of 55 amino acid residues including a signal peptide of 23 residues, a mature toxin of 30 residues cross-linked by three disulfide bridges, and an extra Gly-Lys tail which should be removed in the processing step. The four peptides displayed 24-97% sequence identity with each other, and less than 27% homology with any other scorpion toxins described. However, they shared a common disulfide bridge pattern, which was consistent with that of most short-chain K(+)-toxins, suggesting they represent a new class of scorpion toxins and their target receptors may be a subfamily of K(+) channels. We classified the BmKK toxin subfamily as alpha-KTx14 according to the classification rules. The genomic sequence of BmKK(2) was also cloned and sequenced. It consisted of two exons, disrupted by an intron of 79 bp inserted in the region encoding the C-terminal part of the signal peptide. This structure was very similar to that of other K(+)-toxins described previously.
journal_name
Biochimiejournal_title
Biochimieauthors
Zeng XC,Peng F,Luo F,Zhu SY,Liu H,Li WXdoi
10.1016/s0300-9084(01)01326-8keywords:
subject
Has Abstractpub_date
2001-09-01 00:00:00pages
883-9issue
9eissn
0300-9084issn
1638-6183pii
S0300-9084(01)01326-8journal_volume
83pub_type
杂志文章相关文献
BIOCHIMIE文献大全abstract::Glycolipids constitute a class of molecules with various biological activities. Among them, sugar-based bolaamphiphiles characterized by their biocompatibility, biodegradability and lower toxicity, became interesting for the development of efficient and low cost lipid-based drug delivery systems. Their activity seems ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2016.04.001
更新日期:2016-11-01 00:00:00
abstract::The human ribosomal protein SA, known also as a precursor of the cell-surface laminin receptor, LAMR, is a protein of the 40S ribosomal subunit. It is homologous to eubacterial ribosomal protein S2p, but has a eukaryote-specific C-terminal domain (CTD) that is responsible in LAMR for the binding of laminin as well as ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.12.005
更新日期:2011-03-01 00:00:00
abstract::Alteration of the epigenome is associated with a wide range of human diseases. Therefore, deciphering the pathways that regulate the epigenetic modulation of gene expression is a major milestone for the understanding of diverse biological mechanisms and subsequently human pathologies. Although often evoked, little is ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2007.07.022
更新日期:2008-01-01 00:00:00
abstract::Chronic hypobaric hypoxia induced muscle atrophy results in decreased physical performance at high altitude. Curcumin has been shown to have muscle sparing effects under cachectic conditions. However, the protective effects of curcumin under chronic hypobaric hypoxia have not been studied till now. Therefore, the pres...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2018.10.012
更新日期:2019-01-01 00:00:00
abstract::Pseudouridine [Ψ] is a frequent base modification in the ribosomal RNA [rRNA] and may be involved in the modulation of the conformational flexibility of rRNA helix-loop structures during protein synthesis. Helix 69 of 23S rRNA contains pseudouridines at the positions 1911, 1915 and 1917 which are formed by the helix 6...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2010.12.018
更新日期:2011-05-01 00:00:00
abstract::Determination of the physicochemical parameters of unilamellar and multilamellar liposomes from concentration data in light of a recent quantitative biodynamic concept which strictly adheres to the CGS unit system (cm, g, s) and the mass/volume chemical unit, mol.cm-3. ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(87)90093-9
更新日期:1987-05-01 00:00:00
abstract::In order to confirm the presence of a phosphoenolpyruvate (PEP)-dependent sugar-phosphotransferase system (PTS) in the mollicute Acholeplasma florum we studied the ability of cell free extracts of this organism to phosphorylate glucose and/or fructose in the presence of PEP. We also cloned and sequenced a DNA fragment...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(93)90098-d
更新日期:1993-01-01 00:00:00
abstract::The study of modified nucleoside contributions to RNA chemistry, structure and function has been thwarted by the lack of a site-selected method of incorporation which is both versatile and adaptable to present synthetic technologies. A reproducible and versatile site-selected incorporation of nine differently modified...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(96)88115-6
更新日期:1995-01-01 00:00:00
abstract::The effect of alpha-tocopherol on the structure and thermotropic phase behaviour of 1-palmitoyl-2-oleoyl-phosphatidylethanolamine dispersed in excess water was examined by synchrotron X-ray diffraction and differential scanning calorimetry. Small- and wide-angle X-ray scattering intensity profiles were recorded from m...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2006.07.017
更新日期:2006-12-01 00:00:00
abstract::Coenzymes are small organic molecules that supply a varied set of reactive groups to protein enzymes, thereby diversifying catalysis beyond the chemistries of amino acid sidechains. As RNA structures begin with a more limited chemical diversity than proteins, it seems likely that RNA enzymes would also use functional ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(02)01404-9
更新日期:2002-09-01 00:00:00
abstract::The hepatitis E virus (HEV) is the most common cause of acute hepatitis worldwide. Although HEV is a small, naked RNA virus, HEV particles become associated with lipids in the blood of infected patients and in the supernatant of culture systems. The egress of these particles from cells implies the exocytosis pathway b...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2017.05.003
更新日期:2017-10-01 00:00:00
abstract::Cytochromes P450 constitute a superfamily of hemoproteins which have evolved from a common ancestor. Recent advances in molecular biology have offered a powerful approach to the research on the multiplicity of P450. Now, every mammalian P450 species can be characterized by heterologous expression of its cDNA. In addit...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/0300-9084(96)88175-2
更新日期:1995-01-01 00:00:00
abstract::Glycosphingolipids and sphingolipids of cellular plasma membranes (PMs) reach luminal intra-lysosomal vesicles (LVs) for degradation mainly by pathways of endocytosis. After a sorting and maturation process (e.g. degradation of sphingomyelin (SM) and secretion of cholesterol), sphingolipids of the LVs are digested by ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/j.biochi.2016.05.004
更新日期:2016-11-01 00:00:00
abstract::Classic studies suggested that the common mammalian sialic acid N-glycolylneuraminic acid (Neu5Gc) is an oncofetal antigen in humans, being immunogenic in adult humans and yet apparently expressed in human fetuses and tumors. We and others have recently found that the human deficiency of Neu5Gc can be explained by an ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(01)01309-8
更新日期:2001-07-01 00:00:00
abstract::We have proposed an allosteric ATP inhibition mechanism of Pfk-2 determining the structure of different forms of the enzyme together with a kinetic enzyme analysis. Here we complement the mechanism by using hybrid oligomers of the homodimeric enzyme to get insights about the allosteric communication pathways between t...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2016.08.013
更新日期:2016-09-01 00:00:00
abstract::Rhodospirillum salexigens is a moderately halophilic purple phototrophic bacterium which grows optimally in 8% NaCl. The amino acid sequences of the two principal soluble cytochromes c have been determined. One of these is a cytochrome c2, similar in size to mitochondrial cytochrome c. While clearly of the same sequen...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(94)90135-x
更新日期:1994-01-01 00:00:00
abstract::Interleukin-17 (IL-17) is produced exclusively by activated T cells and neutrophils, and stimulates osteoclastic bone resorption via osteoblasts by inducing the expression of "receptor activator of NF-kappaB (RANK) ligand" (RANKL). However, the direct effects of IL-17 on the differentiation of osteoclast precursors in...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2009.12.011
更新日期:2010-04-01 00:00:00
abstract::Sequence analysis of the Lutzomyia (Lu.) ayacuchensis salivary gland cDNA library identified a short peptide containing an RGD (Arg-Gly-Asp) sequence flanked by two cysteine residues in the C-terminal end as the most abundant transcript. In the present study, a recombinant protein of the RGD-containing peptide, design...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.02.011
更新日期:2015-05-01 00:00:00
abstract::Sialate 9(4)-O-acetylesterases (EC 3.1.1.53) have been isolated from equine liver, bovine brain and influenza C virus. In this latter case, the esterase represents the receptor-destroying enzyme of the virus. The kinetic properties of these enzymes were determined with Neu5,9Ac2 and in part with 4-methylumbelliferyl a...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90288-x
更新日期:1988-11-01 00:00:00
abstract::Cell-free extracts of fungal mycelia of two aflatoxin non-producing isolates of Aspergillus parasiticus (SRRC 163 and SRRC 2043) were utilized for the study of enzyme activities involved in the latter stages of aflatoxin biosynthesis. The post-microsomal fractions (105,000 x g supernatant) of both SRRC 163 and SRRC 20...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(88)90103-4
更新日期:1988-06-01 00:00:00
abstract::The effects of thermal injury (72 h post-injury), 72 h-partial (20% less food) or full starvation on the regulation of phosphofructokinase in the mucosa of rat small intestine were studied. Thermal injury and 72 h-partial or full starvation decreased the activity ratio v0.5/V, but the ratios obtained for thermally inj...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(87)90273-2
更新日期:1987-01-01 00:00:00
abstract::Three different hydrogenases (isolated from Clostridium pasteurianum, Desulfovibrio desulfuricans strain Norway 4 and D. baculatus 9974) added to a suspension of TiO2 (anatase) powder are able to catalyze H2 evolution under band gap illumination of the semiconducting particles, and in the presence of EDTA or methanol ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(86)81086-0
更新日期:1986-01-01 00:00:00
abstract::The functions of residues 57-RY-58, G60, L77, 80-GSGR-83, I101, T104, 134-GY-135, N155, V157 and 160-LV-161 in human arsenic (III) methyltransferase (hAS3MT) 5 Å around S-adenosylmethionine (SAM) have not been studied. Herein, sixteen mutants were designed by substituting these residues with Ala. Mutants G60A, G80A, I...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2014.10.014
更新日期:2014-12-01 00:00:00
abstract::We previously reported that the rRNA methyltransferases RsmI and RsmH, which are responsible for cytidine dimethylation at position 1402 of 16S rRNA in the decoding center of the ribosome, contribute to Staphylococcus aureus virulence. Here we evaluated other 16S rRNA methyltransferases, including KsgA (RsmA), RsmB/F,...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2015.10.027
更新日期:2015-12-01 00:00:00
abstract::We are here reporting that calpain-1 is a constitutive component of a distinct lipid raft/caveolin-1 microdomain isolated from bEnd5 cells in association with endothelial nitric oxide synthase (eNOS) and heat shock protein 90 (HSP90). Perturbations in intracellular calcium concentration by Ca2+-ionophore A23187 or pro...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2016.12.002
更新日期:2017-02-01 00:00:00
abstract::In order to identify conserved potential secondary structures within translational start sites, mRNA sequences derived from different species were studied with programs able to depict such features. The potential secondary structure of 71 bases around the initiator AUG or AUGs in the coding sequences of 290 eukaryotic...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(94)90120-1
更新日期:1994-01-01 00:00:00
abstract::Epidemiological studies have demonstrated that the diabetes mellitus is a serious health burden for both governments and healthcare providers. The present study was hypothesized to evaluate the antihyperglycemic potential of fraxetin by determining the activities of key enzymes of carbohydrate metabolism in streptozot...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2013.06.013
更新日期:2013-10-01 00:00:00
abstract::Multiple forms of acetylcholinesterase have been studied in Goldfish adapted to 15 degrees C and 25 degrees C. Three forms of enzyme (sedimenting at 18S, 12S and 5S) could be distinguished by sucrose gradient centrifugation. Their molecular and enzymatic properties did not vary with environmental temperature. It was n...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80257-x
更新日期:1976-01-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) and αvβ3 integrin are key molecules that actively participate in tumor angiogenesis and metastasis. Some integrin-blocking molecules are currently under clinical trials for cancer and metastasis treatment. However, the mechanism of action of such inhibitors is not completely u...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/j.biochi.2012.04.020
更新日期:2012-08-01 00:00:00
abstract::The dissociation of bound polyribosomes from ewe lactating mammary gland, by EDTA or Puromycin-KC1 treatment releases Messenger Ribonucleoprotein particles (mRNP'S) which sediment in sucrose gradients as a single component having a sedimentation coefficient of about 22S. RNAs derived from mRNPs sediment between 9S and...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(76)80439-7
更新日期:1976-01-01 00:00:00